##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934179.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1560972
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01006039826467	33.0	31.0	34.0	30.0	34.0
2	32.24911209169671	33.0	31.0	34.0	30.0	34.0
3	32.29192708133137	34.0	31.0	34.0	30.0	34.0
4	35.72146009025146	37.0	35.0	37.0	35.0	37.0
5	29.437713809088184	37.0	35.0	37.0	0.0	37.0
6	23.598708368888104	35.0	10.0	37.0	0.0	37.0
7	30.342615370423044	35.0	19.0	37.0	17.0	37.0
8	34.240542431254376	35.0	32.0	37.0	32.0	37.0
9	36.905823422841664	38.0	37.0	39.0	34.0	39.0
10	37.093058043321726	39.0	37.0	39.0	34.0	39.0
11	37.23293114802828	39.0	37.0	39.0	34.0	39.0
12	37.14801226415336	39.0	37.0	39.0	34.0	39.0
13	37.07405385874955	39.0	37.0	39.0	33.0	39.0
14	38.094522515458316	40.0	38.0	41.0	33.0	41.0
15	38.013241749371545	40.0	37.0	41.0	33.0	41.0
16	38.25972919437376	40.0	37.0	41.0	33.0	41.0
17	38.26110141629702	40.0	37.0	41.0	34.0	41.0
18	38.29093603216457	40.0	37.0	41.0	34.0	41.0
19	38.23909589665926	40.0	37.0	41.0	34.0	41.0
20	38.13039952029889	40.0	37.0	41.0	34.0	41.0
21	37.972568374064366	40.0	36.0	41.0	33.0	41.0
22	38.019242497623274	40.0	36.0	41.0	34.0	41.0
23	37.959152374289864	40.0	36.0	41.0	34.0	41.0
24	37.943669713486216	40.0	36.0	41.0	34.0	41.0
25	37.71550995149176	40.0	35.0	41.0	33.0	41.0
26	37.59240011992528	40.0	35.0	41.0	33.0	41.0
27	36.709488703192626	39.0	35.0	41.0	32.0	41.0
28	37.040645828368476	39.0	35.0	41.0	32.0	41.0
29	37.306371927235084	39.0	35.0	41.0	32.0	41.0
30	37.259353787255634	39.0	35.0	41.0	33.0	41.0
31	37.22132171493146	39.0	35.0	41.0	32.0	41.0
32	37.11429352992879	39.0	35.0	41.0	32.0	41.0
33	35.76227504401104	39.0	35.0	41.0	29.0	41.0
34	36.07556573724577	39.0	35.0	41.0	28.0	41.0
35	36.29442872774143	39.0	35.0	41.0	30.0	41.0
36	36.36503281288838	39.0	35.0	41.0	30.0	41.0
37	36.42605056336693	39.0	35.0	41.0	30.0	41.0
38	36.372851659094465	39.0	35.0	41.0	30.0	41.0
39	36.35511463370259	39.0	35.0	41.0	30.0	41.0
40	36.27026301560822	39.0	35.0	41.0	30.0	41.0
41	36.12005212137053	39.0	35.0	41.0	30.0	41.0
42	36.11281304213016	39.0	35.0	41.0	30.0	41.0
43	35.991919778189484	39.0	35.0	41.0	30.0	41.0
44	36.038200557088786	39.0	35.0	40.0	30.0	41.0
45	35.96584948352693	39.0	35.0	40.0	30.0	41.0
46	35.90992983858775	38.0	35.0	40.0	30.0	41.0
47	35.84137127379607	38.0	35.0	40.0	30.0	41.0
48	35.7560398264671	38.0	35.0	40.0	29.0	41.0
49	35.752968663115034	38.0	35.0	40.0	30.0	41.0
50	35.63783334998962	38.0	35.0	40.0	29.0	41.0
51	34.900374253990464	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	14.0
10	25.0
11	19.0
12	30.0
13	37.0
14	54.0
15	74.0
16	132.0
17	206.0
18	398.0
19	758.0
20	1342.0
21	2426.0
22	4034.0
23	6580.0
24	10112.0
25	14679.0
26	18817.0
27	20410.0
28	21431.0
29	23258.0
30	28686.0
31	37476.0
32	52143.0
33	83045.0
34	111968.0
35	134374.0
36	172094.0
37	254302.0
38	297061.0
39	264811.0
40	172.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.387479083545383	4.360680396573417	51.672291367173784	20.579549152707415
2	23.960391345904988	4.936091102210674	49.97360618896431	21.129911362920026
3	20.32592512870186	4.978244324690001	52.68922184382552	22.006608702782625
4	17.78520050327616	5.67684750270985	51.95025919747439	24.587692796539592
5	15.21833831740736	23.31463985260466	41.20323746998665	20.26378436000133
6	16.31143928270334	29.867031567510498	40.270741563589866	13.550787586196291
7	80.28843566700749	2.100229856781544	12.818487455252239	4.792847020958736
8	81.42497110774569	3.207424604669398	11.086489700007432	4.281114587577484
9	76.24057318132547	4.559402731118816	13.29389636713535	5.906127720420353
10	40.27977439697829	26.26549355145384	18.994254861714367	14.460477189853501
11	30.974738816583514	21.77931442716461	27.789223637579664	19.456723118672212
12	27.39722429358118	19.770373843989514	31.848617399927736	20.98378446250157
13	25.367335224462707	19.31719467101268	34.91081198125271	20.40465812327191
14	21.066425278608456	20.98115789392763	36.39174821841776	21.560668609046157
15	20.035913520550018	21.272835130931238	37.52418364967469	21.167067698844054
16	24.22778883926169	22.018588417985715	33.29822700214994	20.45539574060265
17	24.460272189379438	22.073105731557003	32.091350773748665	21.375271305314893
18	24.448420599472637	21.484754370994484	31.86834869555636	22.198476333976522
19	23.98057108007062	24.171221520949768	30.118285273534696	21.729922125444915
20	24.56962712976274	26.452108045499855	29.376824183905924	19.601440640831484
21	24.698649303126512	25.201092652526757	30.781910245667444	19.318347798679284
22	23.690815722511356	21.857791171142082	32.462914132988935	21.988478973357626
23	22.527373969552304	23.54090912585235	32.05195224513957	21.879764659455773
24	22.24229518530762	23.74776741671215	31.332464643824487	22.677472754155744
25	23.984286713663025	24.309468715646403	28.908782476559473	22.79746209413109
26	22.688555592284807	23.834828555541034	29.217372252673336	24.259243599500824
27	21.616082799691476	24.999551561462987	30.45640793044334	22.9279577084022
28	21.142147328715698	24.585514666502668	29.81219394069849	24.46014406408315
29	23.192600507888674	24.48384724389675	28.73395550977212	23.58959673844246
30	25.32127418044654	23.147372278298395	30.067803906796538	21.463549634458527
31	25.363235214981433	24.18127935670851	28.584881727538995	21.870603700771056
32	25.7460736002952	24.70953995331114	27.5044651665757	22.03992127981796
33	24.71940560112545	26.20597935132725	27.01457809621185	22.060036951335448
34	23.662756282623903	24.483462868007884	30.2313558475104	21.622425001857817
35	23.7765315457292	25.935699038804028	28.420304784454814	21.86746463101196
36	25.763434577942462	26.757430626558325	27.24084736945954	20.238287426039673
37	24.25245295879747	27.017589040674654	27.47083227629964	21.259125724228237
38	24.404281434900817	25.07892518251448	28.603972396686167	21.912820985898527
39	24.417670528363097	22.96959842969637	28.645677180628482	23.967053861312053
40	25.911995858990423	22.77215734811387	29.43614619608808	21.87970059680763
41	23.711572020510296	23.63078902119961	28.50883936419103	24.14879959409906
42	22.688555592284807	23.82175977531948	30.53033622640252	22.95934840599319
43	22.42532217105752	23.435333881709603	30.860515114941204	23.278828832291676
44	23.65096875536525	23.010534461860942	29.220767573025014	24.1177292097488
45	22.862357556701852	22.307318773174664	28.42837667812107	26.40194699200242
46	23.228219340257226	23.07844086889451	28.707561698736427	24.985778092111836
47	21.238497551525587	24.22567477187291	31.12778448300162	23.408043193599884
48	22.010644649615752	24.482822241526435	29.79002826444036	23.71650484441745
49	22.925779578365272	22.880935724663864	30.972048185361427	23.221236511609433
50	21.881045912418674	23.15063947335378	30.05665700601933	24.91165760820822
51	21.442601148515156	22.665621164248943	29.895155070046098	25.9966226171898
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	399.0
1	991.0
2	1583.0
3	12540.5
4	23498.0
5	15847.0
6	8196.0
7	7986.0
8	7776.0
9	7770.5
10	7765.0
11	7520.5
12	7276.0
13	7156.0
14	7036.0
15	6666.5
16	6297.0
17	5920.0
18	5543.0
19	5476.5
20	5410.0
21	5712.5
22	6015.0
23	6375.5
24	6736.0
25	7777.0
26	10847.0
27	12876.0
28	15199.0
29	17522.0
30	20546.0
31	23570.0
32	26753.5
33	29937.0
34	33765.5
35	37594.0
36	39686.0
37	41778.0
38	46099.0
39	50420.0
40	54083.5
41	57747.0
42	65648.5
43	73550.0
44	83954.5
45	94359.0
46	111308.5
47	128258.0
48	135331.5
49	142405.0
50	139613.0
51	136821.0
52	125377.5
53	113934.0
54	105684.5
55	97435.0
56	92209.5
57	86984.0
58	82839.5
59	78695.0
60	72509.5
61	66324.0
62	59575.5
63	52827.0
64	46006.5
65	39186.0
66	33267.5
67	27349.0
68	23390.0
69	19431.0
70	15596.0
71	11761.0
72	9598.0
73	7435.0
74	6262.0
75	4033.5
76	2978.0
77	2204.5
78	1431.0
79	1000.0
80	569.0
81	388.0
82	207.0
83	160.5
84	114.0
85	70.5
86	27.0
87	19.0
88	11.0
89	5.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1560972.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.3698097033367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.73569889202909	27.524851154230173
2	8.413482359013287	6.456474341154404
3	4.005673945351552	4.61090841050259
4	2.7544054002098948	4.227440442075865
5	2.152022360099145	4.128634421716486
6	1.7855352282345638	4.110638815557845
7	1.4626222539449942	3.9284376286214386
8	1.203409227226352	3.6939666435131677
9	1.0472650368123098	3.616502415430161
>10	5.3390208691558	30.778350785490023
>50	0.06670142102332093	1.7334380389079684
>100	0.027673960731308175	1.8509760021378205
>500	0.0040079402805197235	1.0775857975316283
>1k	0.0022902515888684138	1.6976278598965826
>5k	1.9085429907236777E-4	0.5641672432336844
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7704	0.49353864130810804	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3423	0.21928644459990312	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTT	2553	0.1635519407138629	No Hit
TTGAATCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCT	2354	0.15080347373303302	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTC	2071	0.13267374430803372	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCT	1770	0.11339088721642669	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTG	1744	0.11172525836465996	No Hit
TTGAATGATACCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGC	1691	0.10832993801298166	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTC	1657	0.10615180797605595	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGC	1629	0.10435805382799947	No Hit
CCTGTCTCTTATACACATCTGACGCGAACTGGTTCGTATGCCGTCTTCTGC	1574	0.10083460818003141	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.406264814487383E-5	0.0	0.0	0.08712520147702842	0.0
2	6.406264814487383E-5	0.0	0.0	0.32550231522410394	0.0
3	6.406264814487383E-5	0.0	0.0	0.5458778248424699	0.0
4	6.406264814487383E-5	0.0	0.0	0.8197456456618056	0.0
5	6.406264814487383E-5	0.0	0.0	1.4597315006290952	0.0
6	6.406264814487383E-5	0.0	0.0	2.4049758740067086	0.0
7	6.406264814487383E-5	0.0	0.0	3.614798984222651	0.0
8	6.406264814487383E-5	0.0	0.0	4.893233190601753	0.0
9	6.406264814487383E-5	0.0	0.0	5.810097810851188	0.0
10	6.406264814487383E-5	0.0	0.0	6.9669411110513195	0.0
11	6.406264814487383E-5	0.0	0.0	8.194381449507102	0.0
12	1.2812529628974766E-4	0.0	0.0	9.347957554651845	0.0
13	1.2812529628974766E-4	0.0	0.0	9.693255228152715	0.0
14	1.2812529628974766E-4	0.0	0.0	9.973337125842104	0.0
15	1.2812529628974766E-4	0.0	0.0	10.180515729942625	0.0
16	1.2812529628974766E-4	0.0	0.0	10.496280522648709	0.0
17	1.2812529628974766E-4	0.0	0.0	10.908779914053552	0.0
18	1.2812529628974766E-4	0.0	0.0	11.318716799532599	0.0
19	1.2812529628974766E-4	0.0	0.0	11.662092593589122	0.0
20	1.2812529628974766E-4	0.0	0.0	11.995602739831336	0.0
21	1.2812529628974766E-4	0.0	0.0	12.471844466140327	0.0
22	1.2812529628974766E-4	0.0	0.0	12.969034678392694	0.0
23	1.2812529628974766E-4	0.0	0.0	13.422406039313966	0.0
24	1.2812529628974766E-4	0.0	0.0	13.815110072442042	0.0
25	1.2812529628974766E-4	0.0	0.0	14.15092647401747	0.0
26	1.921879444346215E-4	0.0	0.0	14.459067811594315	0.0
27	1.921879444346215E-4	0.0	0.0	14.812373316113293	0.0
28	1.921879444346215E-4	0.0	0.0	15.131661554467344	0.0
29	1.921879444346215E-4	0.0	0.0	15.497779588615298	0.0
30	1.921879444346215E-4	0.0	0.0	15.916941495427208	0.0
31	1.921879444346215E-4	0.0	0.0	16.266787616946363	0.0
32	2.562505925794953E-4	0.0	0.0	16.62951033074264	0.0
33	2.562505925794953E-4	0.0	0.0	16.98883772418724	0.0
34	2.562505925794953E-4	0.0	0.0	17.385513641500296	0.0
35	2.562505925794953E-4	0.0	0.0	17.81101775047855	0.0
36	2.562505925794953E-4	0.0	0.0	18.192126444292402	0.0
37	3.2031324072436917E-4	0.0	0.0	18.595721127605106	0.0
38	3.2031324072436917E-4	0.0	0.0	19.101751985301465	0.0
39	3.2031324072436917E-4	0.0	0.0	19.96076803427608	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAT	2205	0.0	43.367348	1
CGTTATT	1675	0.0	42.44776	1
CGTTTTT	4815	0.0	42.383175	1
CTCGTAG	115	0.0	41.08696	1
TTAGGGA	2780	0.0	40.953236	4
TCGTTAG	210	0.0	40.714287	1
CGTTAGG	395	0.0	40.443035	2
TTATGGG	3770	0.0	39.38992	3
TAGGGAC	1170	0.0	39.03846	5
TTACGGG	1385	0.0	38.501804	3
GGGCGAT	3175	0.0	38.40945	7
TATGGGA	2360	0.0	38.135593	4
TAGGGAA	1275	0.0	38.11765	5
AGGGCGA	1010	0.0	38.09406	6
GTTTATT	2485	0.0	37.756542	2
TAGGGAT	1540	0.0	37.694805	5
TAGGGCG	695	0.0	37.553955	5
ACACGAC	540	0.0	37.5	26
TATCGCG	30	1.1401093E-4	37.499996	1
CTAGGGA	1850	0.0	37.459457	4
>>END_MODULE