##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934178.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1165926
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09382842478854	33.0	31.0	34.0	30.0	34.0
2	32.312942673891826	34.0	31.0	34.0	30.0	34.0
3	32.3963913661759	34.0	31.0	34.0	30.0	34.0
4	35.769030796122564	37.0	35.0	37.0	35.0	37.0
5	30.431618301676092	37.0	35.0	37.0	0.0	37.0
6	24.510758830320277	35.0	17.0	37.0	0.0	37.0
7	30.761742168885505	35.0	23.0	37.0	17.0	37.0
8	34.517189770191244	35.0	32.0	37.0	32.0	37.0
9	37.11657000530051	39.0	37.0	39.0	34.0	39.0
10	37.11361012620012	39.0	37.0	39.0	34.0	39.0
11	37.22539680905992	39.0	37.0	39.0	34.0	39.0
12	37.055687925305726	39.0	35.0	39.0	34.0	39.0
13	36.90303844326312	39.0	35.0	39.0	33.0	39.0
14	37.597380965858896	40.0	37.0	41.0	32.0	41.0
15	37.58083274581749	40.0	36.0	41.0	32.0	41.0
16	37.95387957726305	40.0	37.0	41.0	33.0	41.0
17	38.057055078967274	40.0	36.0	41.0	33.0	41.0
18	38.15448579069341	40.0	37.0	41.0	34.0	41.0
19	38.157427658359104	40.0	37.0	41.0	34.0	41.0
20	38.05348109571276	40.0	36.0	41.0	33.0	41.0
21	37.92931283803603	40.0	36.0	41.0	33.0	41.0
22	38.09134713523843	40.0	36.0	41.0	34.0	41.0
23	38.093176582390306	40.0	36.0	41.0	34.0	41.0
24	38.045513180081755	40.0	36.0	41.0	34.0	41.0
25	37.810006810037684	40.0	36.0	41.0	34.0	41.0
26	37.70695824606364	40.0	35.0	41.0	33.0	41.0
27	37.00494971378973	39.0	35.0	41.0	33.0	41.0
28	37.28602415590698	39.0	35.0	41.0	33.0	41.0
29	37.50682290299727	40.0	35.0	41.0	33.0	41.0
30	37.495627509807655	39.0	35.0	41.0	33.0	41.0
31	37.48296461353465	39.0	35.0	41.0	33.0	41.0
32	37.52967769824157	39.0	35.0	41.0	33.0	41.0
33	36.503764389849785	39.0	35.0	41.0	31.0	41.0
34	36.815658969780245	39.0	35.0	41.0	31.0	41.0
35	37.06478112676105	39.0	35.0	41.0	31.0	41.0
36	37.15622174992238	39.0	35.0	41.0	32.0	41.0
37	37.192179435058485	39.0	35.0	41.0	32.0	41.0
38	37.14991345934476	39.0	35.0	41.0	32.0	41.0
39	37.183750941311885	39.0	35.0	41.0	33.0	41.0
40	37.09544345009889	39.0	35.0	41.0	32.0	41.0
41	36.955297334479205	39.0	35.0	41.0	32.0	41.0
42	37.03559145263078	39.0	35.0	41.0	32.0	41.0
43	36.93805438767126	39.0	35.0	41.0	32.0	41.0
44	36.96177716253004	39.0	35.0	41.0	32.0	41.0
45	36.91152268668852	39.0	35.0	41.0	32.0	41.0
46	36.88415388283647	39.0	35.0	41.0	32.0	41.0
47	36.813717165583405	39.0	35.0	40.0	32.0	41.0
48	36.722506402636185	39.0	35.0	40.0	32.0	41.0
49	36.733252367645974	39.0	35.0	40.0	32.0	41.0
50	36.634579724613744	38.0	35.0	40.0	32.0	41.0
51	35.84208603290432	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	5.0
10	11.0
11	11.0
12	15.0
13	20.0
14	20.0
15	30.0
16	49.0
17	103.0
18	199.0
19	333.0
20	571.0
21	1033.0
22	1570.0
23	2322.0
24	3750.0
25	5285.0
26	7275.0
27	8908.0
28	11243.0
29	14492.0
30	19320.0
31	27450.0
32	40459.0
33	67135.0
34	91338.0
35	95078.0
36	121755.0
37	189210.0
38	233992.0
39	222799.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.288035432780468	4.059863147403866	55.36423409375896	18.287867326056713
2	19.507241454431927	3.988846633491319	55.646842080886785	20.85706983118997
3	21.01565622518067	3.9045359654043224	53.27533651363808	21.80447129577692
4	19.104471467314394	4.084564543547361	52.243109768544485	24.56785422059376
5	16.08678423845081	19.834792259543057	43.50370435173416	20.57471915027197
6	16.588359810142325	28.48379742796713	40.804476441901116	14.123366319989433
7	84.63881927326435	1.8398251690072955	8.9884778279239	4.532877729804465
8	87.7213476670046	1.8150380041271916	6.471680020858956	3.991934308009256
9	83.42999469949208	3.5615467877035076	8.418973416837776	4.58948509596664
10	44.559603268131944	21.12638366414335	17.235828002806354	17.078185064918358
11	32.991373380471835	24.176148400498832	23.01732700016982	19.815151218859516
12	29.457529894693145	20.510049522868517	28.606875565001555	21.425545017436782
13	28.5926379547244	19.611107394465858	31.05076994594854	20.7454847048612
14	22.18519871758585	21.28531313308049	35.25944185136964	21.270046297964022
15	20.224353861222756	21.896329612685538	36.412002133926165	21.46731439216554
16	26.612923976307247	22.058518293613833	29.561481603463687	21.76707612661524
17	28.04783493978177	21.61217778829874	27.475843235333976	22.86414403658551
18	27.34187246875016	20.870363985364424	30.06837483682498	21.71938870906044
19	27.991227573619593	22.343098961683676	27.904858455853976	21.76081500884276
20	24.83819727838645	28.053838751344422	27.800735209610217	19.30722876065891
21	25.003902477515727	23.801682096462383	31.77740268250301	19.417012743518885
22	23.335443244253923	20.623864636349136	33.11127807425171	22.929414045145233
23	22.910373385617955	22.41969044347583	31.961376622530075	22.70855954837614
24	23.244785689657835	25.719042203364534	27.9802491753336	23.055922931644034
25	26.282199727941567	23.75528120995672	25.876342066306098	24.086176995795615
26	23.2930734883689	23.762743090041734	26.587965273953923	26.356218147635442
27	22.146774323584857	24.63844189082326	30.616179757548935	22.598604028042946
28	20.79986208387153	23.338445150035252	29.08795240864343	26.77374035744979
29	23.70364843051789	25.065141355454806	28.192355260968533	23.03885495305877
30	27.288524314579142	22.115640272195662	28.875760554271885	21.72007485895331
31	25.168063839386036	22.618588143672927	30.109114986714424	22.10423303022662
32	28.031281573616162	22.54782893597021	26.97589726963804	22.44499222077559
33	27.59471870427454	23.77003343265353	27.172050370263634	21.46319749280829
34	23.483480083641673	22.02026543708606	32.188921080754696	22.307333398517574
35	23.913781835210813	25.137787475362934	28.058041419438283	22.890389269987978
36	28.475735166725848	23.135687856690733	27.097688875623327	21.290888100960096
37	23.748162404818142	25.4244265931114	29.27166904246067	21.555741959609787
38	24.883654708789408	23.49831807507509	28.56073198470572	23.05729523142978
39	23.89937268746044	21.585417942476624	28.96616080265815	25.549048567404792
40	26.106545355365608	20.80946818237178	29.522113753359992	23.561872708902623
41	22.516694884581014	21.6603798182732	28.457037582145006	27.36588771500078
42	21.968032276490963	21.615951612709555	31.6810843912907	24.734931719508786
43	22.295583081602093	21.499306130920832	31.953228592552186	24.251882194924892
44	24.130004820202995	21.4275176983788	29.69931196319492	24.743165518223282
45	23.221113518353654	20.74934429800862	28.09989656290365	27.929645620734078
46	23.608873976564553	20.742225492870045	29.81312707667553	25.83577345388987
47	20.955703878290734	22.8631148117462	32.526678365522336	23.65450294444073
48	21.537644756185212	25.129982520331478	30.11940723510755	23.212965488375765
49	25.102364987143268	21.291145407169925	30.554940879609855	23.051548726076955
50	22.81233971967346	22.063235574127347	28.71605916670526	26.40836553949393
51	22.085792751855607	23.049061432715284	27.311681873463666	27.553463941965443
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	236.0
1	854.5
2	1473.0
3	2916.5
4	4360.0
5	3082.0
6	1804.0
7	1800.5
8	1797.0
9	1845.5
10	1894.0
11	1885.0
12	1876.0
13	1835.5
14	1795.0
15	1755.5
16	1716.0
17	1807.0
18	1898.0
19	1982.0
20	2066.0
21	2339.5
22	2613.0
23	3010.0
24	3407.0
25	4460.5
26	6676.0
27	7838.0
28	8930.5
29	10023.0
30	12351.0
31	14679.0
32	16838.0
33	18997.0
34	21484.0
35	23971.0
36	25580.0
37	27189.0
38	31722.0
39	36255.0
40	41029.0
41	45803.0
42	51032.5
43	56262.0
44	63708.0
45	71154.0
46	83206.5
47	95259.0
48	107824.0
49	120389.0
50	120763.5
51	121138.0
52	109795.0
53	98452.0
54	93192.5
55	87933.0
56	82353.5
57	76774.0
58	70480.5
59	64187.0
60	55405.5
61	46624.0
62	41345.0
63	36066.0
64	31441.5
65	26817.0
66	22849.0
67	18881.0
68	15155.5
69	11430.0
70	9623.5
71	7817.0
72	6291.0
73	4765.0
74	3831.5
75	1980.5
76	1063.0
77	801.5
78	540.0
79	366.0
80	192.0
81	122.5
82	53.0
83	38.0
84	23.0
85	13.5
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1165926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.07604749541958
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.21292526317194	25.33004826586536
2	9.932147972964273	7.16625284008389
3	4.131677226829606	4.471637516125449
4	2.739974369484042	3.9538978195895456
5	1.9255323451787383	3.473279816931741
6	1.5716738857166592	3.401986904905491
7	1.286182406513835	3.248026430961631
8	1.0374586400519472	2.994192573843796
9	0.8745816576540173	2.8396304478134202
>10	6.162642085481311	36.95423729087457
>50	0.0819734731669611	1.9976515390384315
>100	0.039232461263134205	2.6031661078440353
>500	0.0024988828272600157	0.6357364993412543
>1k	0.0014993296963560095	0.9302559467813958
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	2721	0.23337673231405767	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	2166	0.18577508349586508	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	1424	0.12213468093172294	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	1399	0.11999046251648905	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1356	0.11630240684228674	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1253	0.10746822697152306	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.2884368304678E-4	0.0	0.0	0.198040012831003	0.0
2	4.2884368304678E-4	0.0	0.0	0.7175412504738723	0.0
3	4.2884368304678E-4	0.0	0.0	0.9944885009854828	0.0
4	4.2884368304678E-4	0.0	0.0	1.3027413403595083	0.0
5	4.2884368304678E-4	0.0	0.0	1.8984052161114857	0.0
6	4.2884368304678E-4	0.0	0.0	2.4092438113568098	0.0
7	4.2884368304678E-4	0.0	0.0	3.033725982609531	0.0
8	4.2884368304678E-4	0.0	0.0	3.9664609932362773	0.0
9	4.2884368304678E-4	0.0	0.0	4.441791331525328	0.0
10	4.2884368304678E-4	0.0	0.0	5.295619104471467	0.0
11	4.2884368304678E-4	0.0	0.0	6.8477759308909825	0.0
12	4.2884368304678E-4	0.0	0.0	8.136536967183167	0.0
13	4.2884368304678E-4	0.0	0.0	8.561092213399478	0.0
14	4.2884368304678E-4	0.0	0.0	8.767880637364636	0.0
15	4.2884368304678E-4	0.0	0.0	9.010520393232504	0.0
16	5.14612419656136E-4	0.0	0.0	9.516727476700924	0.0
17	5.14612419656136E-4	0.0	0.0	10.211968855656362	0.0
18	5.14612419656136E-4	0.0	0.0	10.928137806344486	0.0
19	5.14612419656136E-4	0.0	0.0	11.459046285956399	0.0
20	5.14612419656136E-4	0.0	0.0	12.064058953998796	0.0
21	5.14612419656136E-4	0.0	0.0	12.880234251573427	0.0
22	5.14612419656136E-4	0.0	0.0	13.767082988114169	0.0
23	5.14612419656136E-4	0.0	0.0	14.573137574768896	0.0
24	5.14612419656136E-4	0.0	0.0	15.17317565608795	0.0
25	5.14612419656136E-4	0.0	0.0	15.733331274883655	0.0
26	5.14612419656136E-4	0.0	0.0	16.25334712494618	0.0
27	5.14612419656136E-4	0.0	0.0	16.885634251230353	0.0
28	5.14612419656136E-4	0.0	0.0	17.447848319704683	0.0
29	5.14612419656136E-4	0.0	0.0	18.103979154766254	0.0
30	5.14612419656136E-4	0.0	0.0	18.842876820655857	0.0
31	5.14612419656136E-4	0.0	0.0	19.40440473923731	0.0
32	5.14612419656136E-4	0.0	0.0	19.948607373023673	0.0
33	6.00381156265492E-4	0.0	0.0	20.502587642783503	0.0
34	6.00381156265492E-4	0.0	0.0	21.137362062429347	0.0
35	6.00381156265492E-4	0.0	0.0	21.824798486353338	0.0
36	6.00381156265492E-4	0.0	0.0	22.403994764676316	0.0
37	6.00381156265492E-4	0.0	0.0	23.03671073464354	0.0
38	6.00381156265492E-4	0.0	0.0	23.812917800958207	0.0
39	6.00381156265492E-4	0.0	0.0	25.423225830798867	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCAT	35	1.2119563E-7	45.000004	36
TATTTCG	20	7.0333283E-4	45.0	1
TCGTTAG	160	0.0	42.1875	1
CGTTAGG	300	0.0	42.0	2
CGTTATT	205	0.0	41.707317	1
CGAGGGT	130	0.0	41.53846	4
TAACGAA	55	6.184564E-11	40.909092	19
CGATAAC	55	6.184564E-11	40.909092	16
TAGGGAC	820	0.0	40.609756	5
TACGGGC	330	0.0	40.227272	4
GCGTTAG	90	0.0	40.0	1
TTAGGGA	1950	0.0	39.461536	4
TTATGGG	2910	0.0	39.43299	3
CTCGTAG	115	0.0	39.130432	1
TCGTTCA	75	0.0	39.0	16
TATGGGC	910	0.0	38.571426	4
TAGGGCA	850	0.0	38.38235	5
CTAGGGA	1000	0.0	38.250004	4
TAGGGCG	490	0.0	38.112244	5
TTGTTAG	615	0.0	38.04878	1
>>END_MODULE