##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934177.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	397631
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85486795546625	31.0	31.0	34.0	30.0	34.0
2	31.994879674874444	33.0	31.0	34.0	30.0	34.0
3	32.094964929796724	33.0	31.0	34.0	30.0	34.0
4	35.64104408358503	37.0	35.0	37.0	33.0	37.0
5	29.830211930156352	37.0	35.0	37.0	0.0	37.0
6	24.016495192779235	35.0	17.0	37.0	0.0	37.0
7	30.500640040640693	35.0	19.0	37.0	17.0	37.0
8	33.99808113552515	35.0	32.0	37.0	32.0	37.0
9	36.80203756749348	37.0	37.0	39.0	34.0	39.0
10	36.95860232225355	39.0	37.0	39.0	34.0	39.0
11	37.09883032258551	39.0	37.0	39.0	34.0	39.0
12	36.9890954176108	39.0	35.0	39.0	33.0	39.0
13	36.88168679001386	39.0	35.0	39.0	33.0	39.0
14	37.90929278652821	40.0	37.0	41.0	33.0	41.0
15	37.7759203885009	40.0	37.0	41.0	33.0	41.0
16	38.0757360467368	40.0	37.0	41.0	33.0	41.0
17	38.02902188209672	40.0	36.0	41.0	33.0	41.0
18	38.118446499392654	40.0	37.0	41.0	34.0	41.0
19	38.12684876179171	40.0	37.0	41.0	34.0	41.0
20	37.98227502382863	40.0	36.0	41.0	33.0	41.0
21	37.94698099494255	40.0	36.0	41.0	33.0	41.0
22	38.04480535974308	40.0	37.0	41.0	34.0	41.0
23	37.968018087120974	40.0	36.0	41.0	34.0	41.0
24	37.96708003148648	40.0	36.0	41.0	34.0	41.0
25	37.75363339377463	40.0	36.0	41.0	33.0	41.0
26	37.667875492604956	40.0	36.0	41.0	33.0	41.0
27	36.77095347193755	39.0	35.0	41.0	32.0	41.0
28	37.15405237519207	39.0	35.0	41.0	32.0	41.0
29	37.412960760101704	39.0	35.0	41.0	32.0	41.0
30	37.32885765948832	39.0	35.0	41.0	32.0	41.0
31	37.42393072974693	39.0	35.0	41.0	33.0	41.0
32	37.317050229987096	39.0	35.0	41.0	33.0	41.0
33	36.11849428238744	39.0	35.0	41.0	30.0	41.0
34	36.612062439799715	39.0	35.0	41.0	30.0	41.0
35	36.85373373806368	39.0	35.0	41.0	30.0	41.0
36	36.97844483956231	39.0	35.0	41.0	31.0	41.0
37	37.05664296797785	39.0	35.0	41.0	32.0	41.0
38	36.97749672434996	39.0	35.0	41.0	32.0	41.0
39	37.055943827317286	39.0	35.0	41.0	32.0	41.0
40	36.947277752489114	39.0	35.0	41.0	32.0	41.0
41	36.926212996471605	39.0	35.0	40.0	32.0	41.0
42	36.999451753007186	39.0	35.0	41.0	32.0	41.0
43	36.903224346190314	39.0	35.0	40.0	32.0	41.0
44	36.97891009503786	39.0	35.0	40.0	32.0	41.0
45	36.91665136772535	39.0	35.0	40.0	32.0	41.0
46	36.84076945710973	39.0	35.0	40.0	32.0	41.0
47	36.72256187269101	39.0	35.0	40.0	31.0	41.0
48	36.70776926346286	39.0	35.0	40.0	32.0	41.0
49	36.77789206575946	39.0	35.0	40.0	32.0	41.0
50	36.672837379379374	39.0	35.0	40.0	32.0	41.0
51	35.938860400723286	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	5.0
11	8.0
12	5.0
13	4.0
14	7.0
15	7.0
16	12.0
17	26.0
18	42.0
19	103.0
20	246.0
21	518.0
22	834.0
23	1175.0
24	1456.0
25	1887.0
26	2361.0
27	2789.0
28	3590.0
29	4799.0
30	6710.0
31	9607.0
32	13824.0
33	22480.0
34	29988.0
35	38129.0
36	45951.0
37	67581.0
38	78614.0
39	64822.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.985919105904724	4.152342246957606	50.14951047579288	16.71222817134479
2	18.003374988368613	11.237554416028932	49.4780336543177	21.28103694128476
3	19.514826560303398	10.894522811350221	49.051759042931764	20.538891585414618
4	19.297791168193626	4.364599339588715	53.831818947717856	22.505790544499803
5	19.009584262796412	21.29562332916699	39.14785316034213	20.54693924769447
6	16.35108932653641	32.66470672558226	36.808749820813766	14.175454127067558
7	77.47685668370927	1.9784674736124699	14.803423274342292	5.7412525683359705
8	77.14086678352542	6.776886107974479	9.17081414678433	6.911432961715762
9	72.6165716455709	4.45589000857579	11.204860788017031	11.722677557836285
10	41.71556040650754	24.401015011405047	18.44247556151306	15.440949020574351
11	32.47684410923696	22.093096363211117	25.373021721143473	20.057037806408452
12	25.909951688877374	19.726329184595766	31.577769338909693	22.785949787617163
13	24.56548910924953	23.202919289492016	33.855257764107925	18.376333837150526
14	20.020320347256625	28.047863471409425	30.379925106442908	21.551891074891042
15	16.72631158033453	23.018577525394146	38.40570780447198	21.849403089799335
16	19.510048260824732	23.318604434764893	30.520759196340325	26.650588108070046
17	19.061894067615455	23.825104179503107	30.566027296664494	26.546974456216944
18	20.854259351006334	22.038271663929624	32.63528246037155	24.47218652469249
19	21.9467295054963	24.05069021278522	29.108142976779977	24.8944373049385
20	24.434966086648174	25.318448511308222	30.918866989746775	19.32771841229683
21	24.128148962228803	27.89772427200092	28.690419006566394	19.283707759203885
22	20.110353569012478	23.80699693937344	30.080149686518403	26.00249980509568
23	20.812763592375845	25.42709195208623	28.414535084035197	25.345609371502725
24	23.193614179980937	22.99342858076961	28.14669882378386	25.66625841546559
25	20.34851407460686	28.042833682484513	25.444193234430916	26.16445900847771
26	21.005907487092305	24.73122065432524	28.835779906496224	25.42709195208623
27	22.900628975105057	26.054558120468474	28.486712555107623	22.558100349318842
28	19.74745429808038	24.45583971068654	30.7566562969185	25.040049694314575
29	24.5780635815618	22.329747932128026	29.606595059238337	23.485593427071834
30	22.679569751855365	26.226576901700323	29.101101272285106	21.99275207415921
31	25.622750741265143	25.207290176067758	22.985632407936002	26.184326674731096
32	27.67113228093383	28.386871244948203	22.822415757322744	21.11958071679522
33	22.936591965918147	29.140585115345637	23.660630081658624	24.262192837077592
34	24.183979619295275	23.9508489026258	26.45668974501485	25.40848173306407
35	21.37056718414812	26.744645160965817	27.034612492486755	24.85017516239931
36	26.085742811802902	27.31778960895906	26.183320716946113	20.413146862291924
37	22.975824319532432	25.806086547578033	29.78917639721249	21.42891273567705
38	23.979015720605286	29.567865684516548	23.521304928438678	22.93181366643949
39	24.309724342417972	27.115340604731525	25.895113811548896	22.67982124130161
40	22.044055921193266	26.716226853540093	26.132771338250787	25.106945887015854
41	19.67075001697554	26.62367873732179	24.320538388606522	29.38503285709615
42	21.943208653248867	25.24400763521959	27.38921261169275	25.423571099838792
43	23.672701575078403	24.69576064240464	26.64279193523644	24.98874584728052
44	22.970543041161278	21.620044714823543	29.239420467720073	26.16999177629511
45	22.423804985023803	21.822745208497327	26.527358279409807	29.226091527069066
46	25.336304261991643	24.072066815716077	27.993793240466662	22.59783568182561
47	18.8876118813674	23.698604988041676	32.712238230922644	24.701544899668285
48	20.980255563575277	24.15204045962211	28.29583206540737	26.571871911395238
49	22.17960873271953	21.62784088765715	32.908651488440285	23.28389889118303
50	22.528927573554373	22.95696261106403	28.424846151331256	26.08926366405034
51	20.23559531324268	22.343076872779033	26.381745890033727	31.03958192394456
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	191.0
2	314.0
3	680.5
4	1047.0
5	750.0
6	453.0
7	474.0
8	495.0
9	550.0
10	605.0
11	715.0
12	825.0
13	824.0
14	823.0
15	824.5
16	826.0
17	855.5
18	885.0
19	887.0
20	889.0
21	1025.0
22	1161.0
23	1111.0
24	1061.0
25	1362.0
26	2037.5
27	2412.0
28	2694.0
29	2976.0
30	3853.0
31	4730.0
32	5244.5
33	5759.0
34	6600.0
35	7441.0
36	8438.0
37	9435.0
38	10752.0
39	12069.0
40	14340.5
41	16612.0
42	19142.5
43	21673.0
44	25572.5
45	29472.0
46	41523.5
47	53575.0
48	52650.5
49	51726.0
50	48218.0
51	44710.0
52	37939.5
53	31169.0
54	27375.0
55	23581.0
56	21158.0
57	18735.0
58	16915.5
59	15096.0
60	13380.0
61	11664.0
62	9886.0
63	8108.0
64	6967.5
65	5827.0
66	4801.5
67	3776.0
68	3160.5
69	2545.0
70	2029.0
71	1513.0
72	1124.5
73	736.0
74	657.5
75	448.5
76	318.0
77	232.0
78	146.0
79	119.5
80	93.0
81	61.0
82	29.0
83	19.5
84	10.0
85	5.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	397631.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.73226003009082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4782034743628	29.194550869873055
2	10.557824733935181	8.389724753616742
3	4.064106263597591	4.844283805655408
4	2.272054314440956	3.610954112954312
5	1.603262039198834	3.185061211891086
6	1.2276803449551594	2.9267108819753997
7	0.9438754944666174	2.6251614607524734
8	0.749787468367671	2.383260052839024
9	0.6550328827871851	2.3423343144444395
>10	4.328800818451336	28.98476363901722
>50	0.07136379455452745	1.92217383514578
>100	0.03697942081461877	2.9236013997885792
>500	0.004541332380742656	1.2738880371852546
>1k	0.006487617686775222	5.393531624861244
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	4122	1.0366394974234905	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCG	3808	0.9576718113024386	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	2701	0.6792729943087938	No Hit
CCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1964	0.49392527242594264	No Hit
CTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	1757	0.44186695705314727	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGGACAAT	1713	0.43080142141835015	No Hit
GCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1644	0.4134486496274184	No Hit
TCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	1192	0.29977541992450285	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTC	1021	0.2567707246165415	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCT	1002	0.25199242513787906	No Hit
GAATCAGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	972	0.24444774175051745	No Hit
CTGTCACTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGCT	716	0.18006644351169804	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGGACAATCGTA	713	0.17931197517296185	No Hit
CCTGTATCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	712	0.17906048572671646	No Hit
GCTGTATCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	641	0.1612047350432939	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCT	636	0.15994728781206696	No Hit
GAATATGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTC	552	0.13882217432745436	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGC	420	0.10562556742306309	No Hit
TCTGTATCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	419	0.10537407797681769	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTG	417	0.10487109908432692	No Hit
CCTGACTCTTATACACATCTGACGCGTGGACAATCGTATGCCGTCTTCTGC	414	0.10411663074559076	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4758180322962747	0.0
2	0.0	0.0	0.0	1.7961376250845633	0.0
3	0.0	0.0	0.0	2.3773297353576557	0.0
4	0.0	0.0	0.0	3.1408516941586546	0.0
5	0.0	0.0	0.0	4.831866730712646	0.0
6	0.0	0.0	0.0	5.547605694727021	0.0
7	0.0	0.0	0.0	6.662458409932827	0.0
8	0.0	0.0	0.0	8.316755987334991	0.0
9	0.0	0.0	0.0	8.880595325817152	0.0
10	0.0	0.0	0.0	11.154311409321707	0.0
11	0.0	0.0	0.0	13.209483164039021	0.0
12	0.0	0.0	0.0	16.370454013897305	0.0
13	0.0	0.0	0.0	17.068588716674505	0.0
14	0.0	0.0	0.0	17.35352625927053	0.0
15	0.0	0.0	0.0	17.911078361596555	0.0
16	0.0	0.0	0.0	18.782489292836825	0.0
17	0.0	0.0	0.0	19.88929434576278	0.0
18	0.0	0.0	0.0	21.071797722008597	0.0
19	0.0	0.0	0.0	22.448953929648344	0.0
20	0.0	0.0	0.0	23.36839934512148	0.0
21	0.0	0.0	0.0	24.46791120410632	0.0
22	0.0	0.0	0.0	25.543531565697844	0.0
23	0.0	0.0	0.0	26.513274870420062	0.0
24	0.0	0.0	0.0	27.33313046518003	0.0
25	0.0	0.0	0.0	28.02246303733864	0.0
26	0.0	0.0	0.0	28.68061091816282	0.0
27	0.0	0.0	0.0	29.339513267325735	0.0
28	0.0	0.0	0.0	30.00017604261237	0.0
29	0.0	0.0	0.0	30.707112876008157	0.0
30	0.0	0.0	0.0	31.478431007642765	0.0
31	0.0	0.0	0.0	32.184613372699815	0.0
32	0.0	0.0	0.0	32.78089484974763	0.0
33	0.0	0.0	0.0	33.392517183016416	0.0
34	0.0	0.0	0.0	34.041862933222006	0.0
35	0.0	0.0	0.0	34.7266686953482	0.0
36	0.0	0.0	0.0	35.35212294816048	0.0
37	0.0	0.0	0.0	35.96399677087551	0.0
38	0.0	0.0	0.0	36.59297187593523	0.0
39	0.0	0.0	0.0	37.436718968088506	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCC	30	2.161907E-6	45.000004	6
CGAAAAA	30	2.161907E-6	45.000004	22
TACGGGT	60	0.0	45.000004	4
CGAGGGT	30	2.161907E-6	45.000004	4
CCGCAGG	30	2.161907E-6	45.000004	2
GTGCAAC	20	7.027714E-4	45.0	16
ACGTTGG	25	3.886188E-5	45.0	2
TGGACCG	25	3.886188E-5	45.0	1
GTCTATC	20	7.027714E-4	45.0	2
GATCAGT	20	7.027714E-4	45.0	23
TCGATGG	20	7.027714E-4	45.0	2
ACGGGTC	25	3.886188E-5	45.0	5
TCGCTTG	25	3.886188E-5	45.0	1
ACGTGTT	20	7.027714E-4	45.0	40
AAACCCG	20	7.027714E-4	45.0	18
TTGCGGT	20	7.027714E-4	45.0	15
ACTCGGT	20	7.027714E-4	45.0	32
CCTGGTA	20	7.027714E-4	45.0	35
GTCTTAC	50	2.1827873E-11	45.0	31
ATACGGA	20	7.027714E-4	45.0	28
>>END_MODULE