FastQCFastQC Report
Sat 14 Jan 2017
SRR2934175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347127
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC54501.5700305651821955No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG51181.4743883362573353No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC32620.9397137070870315No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGCTTTT19610.5649229244628047No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC18690.5384196562065181TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC16920.4874296727134449TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT15350.4422012692760863TruSeq Adapter, Index 23 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT13350.3845854687189415No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC12080.34799943536515454No Hit
GAATCAGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC12030.3465590403512259No Hit
TCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC11770.3390689862787971TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTA9780.281741264724438No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT7360.21202614605029282No Hit
CCTGTATCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC6600.19013214183857782TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC6370.18350632477450615TruSeq Adapter, Index 23 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC6240.17976129773829175No Hit
CTGTCACTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT5230.15066531845693362TruSeq Adapter, Index 23 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC5040.14519181740400489No Hit
TCCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG5010.14432758039564772TruSeq Adapter, Index 23 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC4940.14231102737614765TruSeq Adapter, Index 23 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG4080.11753623313657538No Hit
GCTGACTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC3770.10860578405021792TruSeq Adapter, Index 20 (95% over 22bp)
CCTGACTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC3550.102268045988932TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGT3530.10169188798336057No Hit
CGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTG3490.10053957197221766TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATCG253.88516E-545.00000443
TCGTTAG600.045.0000041
CCCTACA253.88516E-545.00000427
CGCATGT253.88516E-545.00000438
TCTTACG253.88516E-545.0000041
ATATGTT253.88516E-545.00000440
CAGGGCG502.1827873E-1145.0000045
TGCGCGG253.88516E-545.0000042
TTCCACG253.88516E-545.0000041
GCCCTAC253.88516E-545.00000426
TCATCGG253.88516E-545.00000444
ATACGCG253.88516E-545.00000433
CAATATG253.88516E-545.00000428
TTCGAAT253.88516E-545.00000443
GCGTAGG302.1611067E-645.0000042
TGCGTAG253.88516E-545.0000041
TCCGGGT302.1611067E-645.0000044
TTCTACG406.7921064E-945.01
ATTAGGA207.0264767E-445.027
CTCGTAG207.0264767E-445.01
Produced by FastQC (version 0.11.5)