SRR2934174.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934174.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425838
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC	4666	1.0957218472752548	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG	3980	0.9346277222793644	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	2553	0.5995237625575923	No Hit
CCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1885	0.44265659710969896	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACTAGGGT	1882	0.4419521038516994	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1654	0.38841061624373585	No Hit
CTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	1403	0.32946801365777595	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	1196	0.28085797885580904	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	1046	0.245633315955833	No Hit
GAATCAGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	1028	0.24140635640783584	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTC	1017	0.2388232144618376	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTA	754	0.1770626388438796	No Hit
GCTGTATCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	704	0.16532108454388758	No Hit
GAATATGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	617	0.14489078006190148	No Hit
CCTGTATCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	611	0.14348179354590243	No Hit
CTGTCACTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGCT	528	0.12399081340791569	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	515	0.12093800928991777	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC	497	0.11671104974192063	No Hit
TCTGTATCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	477	0.11201442802192382	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG	461	0.10825713064592639	No Hit
TCCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTG	452	0.10614365087192783	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTAG	60	0.0	45.000004	1
ATTAGTC	60	0.0	45.000004	31
CGTATGG	65	0.0	45.000004	2
GGTCGAA	70	0.0	45.000004	42
CGCGGAA	35	1.2095188E-7	45.000004	40
GATCCGA	30	2.162271E-6	45.000004	32
CCGTGTG	30	2.162271E-6	45.000004	11
CGGTCGA	65	0.0	45.000004	41
GATCTCG	35	1.2095188E-7	45.000004	11
AATAGAG	60	0.0	45.000004	19
TATCGGG	30	2.162271E-6	45.000004	4
CTCTACG	30	2.162271E-6	45.000004	1
ATCTCGT	20	7.028279E-4	45.0	12
ACGCCGG	20	7.028279E-4	45.0	27
TGCTTAG	100	0.0	45.0	1
CAACGAG	20	7.028279E-4	45.0	14
ATCAGTC	160	0.0	45.0	3
CAACCGC	20	7.028279E-4	45.0	16
AGGCGTA	20	7.028279E-4	45.0	13
CTAAGAC	20	7.028279E-4	45.0	31

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content