FastQCFastQC Report
Sat 14 Jan 2017
SRR2934173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences671254
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC23090.3439830526149565TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT21780.3244673402318645TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC17120.2550450351133848No Hit
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC15710.2340395736934156TruSeq Adapter, Index 14 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC14900.2219726064947099TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG14160.21094846362181827No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC10140.15106055233935292No Hit
CTGTCACTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT9060.13497126274107865TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTATCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC8470.12618174342350288TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC7430.1106883534399795TruSeq Adapter, Index 15 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT700.045.06
TCGTTGA207.031188E-445.024
CTCCGTA253.8890652E-545.014
CGTCTAC207.031188E-445.035
CTACGCG207.031188E-445.014
TGCGACG253.8890652E-545.01
ACGTCTA207.031188E-445.034
ACGTATA207.031188E-445.040
CTTTACG1000.045.01
CGGTATT351.2109194E-745.043
CCGTTAG253.8890652E-545.01
AATCGGA207.031188E-445.032
TAGGGCG2400.043.1249965
TTAGGGA12450.042.1084334
GACCGAT2600.041.5384649
CGTATGG1900.041.4473722
ATGGGAC4250.041.2941175
TAGGGAG8050.041.086965
CGTTAGG1700.041.029412
CGTAAGG556.002665E-1140.909092
Produced by FastQC (version 0.11.5)