##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934172.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	855242
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0274191398458	33.0	31.0	34.0	30.0	34.0
2	32.22096318936629	33.0	31.0	34.0	30.0	34.0
3	32.29579580984096	34.0	31.0	34.0	30.0	34.0
4	35.7271930050208	37.0	35.0	37.0	35.0	37.0
5	29.90649430219751	37.0	35.0	37.0	0.0	37.0
6	24.083922445342957	35.0	17.0	37.0	0.0	37.0
7	30.551930330830338	35.0	19.0	37.0	17.0	37.0
8	34.304187586671375	35.0	32.0	37.0	32.0	37.0
9	36.95737463782181	38.0	37.0	39.0	34.0	39.0
10	37.07951199777373	39.0	37.0	39.0	34.0	39.0
11	37.18561413026956	39.0	37.0	39.0	34.0	39.0
12	36.95747636341527	39.0	35.0	39.0	33.0	39.0
13	36.74886757198547	39.0	35.0	39.0	33.0	39.0
14	37.35314682861693	40.0	36.0	41.0	31.0	41.0
15	37.374106977908006	40.0	36.0	41.0	31.0	41.0
16	37.80253542272246	40.0	36.0	41.0	33.0	41.0
17	37.91466041190681	40.0	36.0	41.0	33.0	41.0
18	38.02538112019756	40.0	36.0	41.0	33.0	41.0
19	38.025201054204544	40.0	36.0	41.0	33.0	41.0
20	37.874522065099704	40.0	36.0	41.0	33.0	41.0
21	37.79718839813761	40.0	36.0	41.0	33.0	41.0
22	37.96755888976453	40.0	36.0	41.0	34.0	41.0
23	37.95070518052201	40.0	36.0	41.0	34.0	41.0
24	37.91343970478531	40.0	35.0	41.0	34.0	41.0
25	37.68645833576929	39.0	35.0	41.0	33.0	41.0
26	37.57705421389502	39.0	35.0	41.0	33.0	41.0
27	36.77651822525087	39.0	35.0	41.0	32.0	41.0
28	37.1219116928308	39.0	35.0	41.0	32.0	41.0
29	37.35720299049859	39.0	35.0	41.0	32.0	41.0
30	37.338653854698435	39.0	35.0	41.0	33.0	41.0
31	37.30548429567304	39.0	35.0	41.0	33.0	41.0
32	37.296196865916315	39.0	35.0	41.0	33.0	41.0
33	36.192775845900925	39.0	35.0	41.0	31.0	41.0
34	36.51650643911314	39.0	35.0	41.0	31.0	41.0
35	36.790248841848275	39.0	35.0	41.0	31.0	41.0
36	36.88465019257707	39.0	35.0	41.0	32.0	41.0
37	36.92065286784325	39.0	35.0	41.0	32.0	41.0
38	36.92152864335475	39.0	35.0	41.0	32.0	41.0
39	36.9185259844582	39.0	35.0	41.0	32.0	41.0
40	36.764324015892576	39.0	35.0	40.0	32.0	41.0
41	36.58652755594323	39.0	35.0	40.0	31.0	41.0
42	36.71062693366322	39.0	35.0	40.0	32.0	41.0
43	36.649626655379414	39.0	35.0	40.0	32.0	41.0
44	36.722003830494764	39.0	35.0	40.0	32.0	41.0
45	36.70222229497616	38.0	35.0	40.0	32.0	41.0
46	36.67350410760931	38.0	35.0	40.0	32.0	41.0
47	36.6022447447623	38.0	35.0	40.0	32.0	41.0
48	36.53256505176313	38.0	35.0	40.0	32.0	41.0
49	36.56091959936486	38.0	35.0	40.0	32.0	41.0
50	36.43574801050463	38.0	35.0	40.0	32.0	41.0
51	35.614848195013806	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	2.0
10	5.0
11	18.0
12	17.0
13	8.0
14	22.0
15	23.0
16	49.0
17	72.0
18	157.0
19	280.0
20	501.0
21	924.0
22	1441.0
23	2103.0
24	3053.0
25	4152.0
26	5228.0
27	6556.0
28	8211.0
29	11014.0
30	15069.0
31	22228.0
32	32904.0
33	54127.0
34	72214.0
35	75920.0
36	95233.0
37	141450.0
38	162739.0
39	139422.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.487922716611205	4.09509822950697	53.74981584159805	17.667163212283775
2	19.547917431557384	6.301842051723372	53.669955404435235	20.480285112284008
3	21.26193521833586	6.1244653560044995	51.18177077365237	21.43182865200727
4	20.182942371866677	4.285570633808911	51.39410833424925	24.13737866007516
5	16.840496607977627	21.2853204122342	40.18768956622804	21.686493413560136
6	17.50136218754458	29.93246356002161	39.19744353060303	13.36873072183078
7	82.06414090982436	2.0747344026602996	10.996653578753149	4.864471108762198
8	83.52057078581268	3.5401675782994753	7.765053633942205	5.174208001945648
9	78.44387904242308	4.491594192053244	9.817922880307563	7.246603885216114
10	46.82616148411795	20.95360143678631	17.265522507079865	14.954714572015874
11	35.03476209072988	20.607734419029935	24.021972728186874	20.335530762053313
12	30.946094789544947	17.631150013680337	29.707965698597587	21.714789498177122
13	29.527665853641427	19.265307363296003	31.903016923864826	19.30400985919775
14	22.078896967174202	22.130578245689524	35.68183040589681	20.108694381239463
15	19.60766660196763	20.463915476555176	38.41088253383253	21.517535387644667
16	26.094134759518354	20.62609179624013	29.77005338839767	23.509720055843843
17	26.837433147576945	21.1181162758611	28.007511324280145	24.03693925228181
18	27.148807004333275	19.811702418730608	30.387539433283212	22.65195114365291
19	27.103907432048473	22.164135998933634	28.211897918951596	22.520058650066296
20	24.38491093748904	28.083045500571767	28.52046555244013	19.01157800949907
21	24.61735976483849	23.755264591776363	32.47478491467912	19.152590728706027
22	22.19079511997774	20.4930300429586	33.798620741263875	23.517554095799788
23	21.803302457082324	22.60050371707657	32.20433514724487	23.391858678596233
24	23.146197216694222	25.822515732389196	27.304435469726695	23.726851581189887
25	25.51675432216846	24.310429094922842	25.186087680446	24.986728902462694
26	21.081518447410208	23.5215295787625	27.69812520900517	27.698826764822122
27	20.962721662406665	25.153348408988336	30.285930765794944	23.59799916281006
28	19.148849097682294	23.329069432979203	29.185657392878273	28.336424076460233
29	23.249910551633338	24.466525264194228	28.43394033501629	23.849623849156146
30	26.10115031768786	23.755615369684836	28.401434915497603	21.741799397129704
31	24.83343895645911	23.068324521012766	29.023948777071283	23.074287745456843
32	28.654345787508095	24.825604916503167	25.20549739138162	21.314551904607118
33	28.63493607657248	24.22121458020069	24.96042055932707	22.18342878389976
34	22.868848817059966	22.431896469069574	31.31546392716915	23.383790786701308
35	23.64465262463724	26.547339817268096	26.956346858549978	22.85166069954469
36	27.3367070373064	24.978193306689803	26.04128422130812	21.643815434695675
37	23.548773329654065	27.84814122786299	26.183816978118475	22.41926846436447
38	26.417902769040808	24.28739467893298	24.77439134186581	24.5203112101604
39	26.573765086373214	21.730925281966975	26.412874952352666	25.28243467930714
40	26.35990748817294	21.405754160810623	28.925847888667768	23.30849046234867
41	22.63371069241221	22.98203315552791	27.22632892210626	27.15792722995363
42	22.246451881455776	23.220795985229913	30.108203292167595	24.424548841146716
43	23.55204725679983	21.723675871858493	30.056638939621767	24.66763793171991
44	24.388886420451755	20.95348451081682	28.86516331050159	25.79246575822983
45	22.671594706527507	20.461810809104325	26.98347368347205	29.88312080089612
46	23.312699797250367	21.599149714349856	29.27954894638009	25.808601542019687
47	19.783990963961077	23.480605489440414	32.98200977033401	23.753393776264495
48	21.073567481484773	26.45298055988831	28.373021904911123	24.10043005371579
49	24.998889203289828	21.7446056203975	29.86756964695373	23.388935529358942
50	23.174142523402733	22.492814899174736	27.064854158238255	27.268188419184273
51	21.54980695522437	23.84693455185784	25.94786037168427	28.65539812123352
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	168.0
1	581.0
2	994.0
3	1789.0
4	2584.0
5	1821.5
6	1059.0
7	1146.0
8	1233.0
9	1260.5
10	1288.0
11	1354.0
12	1420.0
13	1343.5
14	1267.0
15	1297.5
16	1328.0
17	1364.5
18	1401.0
19	1466.5
20	1532.0
21	1669.0
22	1806.0
23	2172.5
24	2539.0
25	2909.0
26	4379.5
27	5480.0
28	6505.0
29	7530.0
30	8938.5
31	10347.0
32	11881.5
33	13416.0
34	15153.5
35	16891.0
36	17961.5
37	19032.0
38	21977.0
39	24922.0
40	28364.0
41	31806.0
42	35931.5
43	40057.0
44	46084.0
45	52111.0
46	65807.0
47	79503.0
48	86506.0
49	93509.0
50	91963.5
51	90418.0
52	80086.5
53	69755.0
54	65974.0
55	62193.0
56	57974.0
57	53755.0
58	49265.0
59	44775.0
60	38476.5
61	32178.0
62	28902.0
63	25626.0
64	22580.0
65	19534.0
66	16661.5
67	13789.0
68	11949.0
69	10109.0
70	8525.5
71	6942.0
72	5804.0
73	4666.0
74	3629.0
75	1964.0
76	1336.0
77	961.5
78	587.0
79	406.5
80	226.0
81	205.5
82	185.0
83	122.5
84	60.0
85	37.0
86	14.0
87	7.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	855242.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.89425680281319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.18016551283468	27.352137280129167
2	10.351386446430416	7.845161925323881
3	3.986399896915301	4.531849842372494
4	2.4098633675620906	3.652799252403602
5	1.700335418994648	3.2216473509151076
6	1.3502473963918107	3.06999729517207
7	1.0397233689897374	2.757968104386825
8	0.8861553483172429	2.6864158669055977
9	0.7305031889248302	2.491368789275233
>10	5.221633455542935	33.540819768965
>50	0.09300498266357374	2.4529504172683487
>100	0.043075957220162706	3.068164696319785
>500	0.004242329120161998	1.0038838017293108
>1k	0.0032633300924323062	2.3248356088335846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGC	2927	0.3422423127021358	No Hit
CCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	2741	0.32049408237668403	No Hit
CTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	2586	0.3023705571054742	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCG	2376	0.2778161035122223	No Hit
GCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	1751	0.20473737257992475	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTC	1734	0.20274963109856628	No Hit
TCTGTCTCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	1508	0.17632436199344748	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACGGTAGT	1148	0.1342310129764441	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1027	0.12008297066795129	No Hit
CCTGTATCTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGC	1002	0.1171598214306594	No Hit
CTGTCACTTATACACATCTGACGCTACGGTAGTCGTATGCCGTCTTCTGCT	905	0.10581800238996682	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1692596949167604E-4	0.0	0.0	0.3249372692173677	0.0
2	1.1692596949167604E-4	0.0	0.0	1.0826175515234284	0.0
3	1.1692596949167604E-4	0.0	0.0	1.480165847795127	0.0
4	1.1692596949167604E-4	0.0	0.0	1.942491131165214	0.0
5	1.1692596949167604E-4	0.0	0.0	2.8590738060104623	0.0
6	1.1692596949167604E-4	0.0	0.0	3.4156414207908403	0.0
7	1.1692596949167604E-4	0.0	0.0	4.20115008383592	0.0
8	1.1692596949167604E-4	0.0	0.0	5.300371123027166	0.0
9	1.1692596949167604E-4	0.0	0.0	5.8092329422549405	0.0
10	1.1692596949167604E-4	0.0	0.0	7.002696312856478	0.0
11	1.1692596949167604E-4	0.0	0.0	8.764069117279085	0.0
12	1.1692596949167604E-4	0.0	0.0	10.68703361153919	0.0
13	2.3385193898335207E-4	0.0	0.0	11.237287223967018	0.0
14	2.3385193898335207E-4	0.0	0.0	11.476985461424954	0.0
15	2.3385193898335207E-4	0.0	0.0	11.823320183059298	0.0
16	2.3385193898335207E-4	0.0	0.0	12.467465348988942	0.0
17	2.3385193898335207E-4	0.0	0.0	13.2912088040578	0.0
18	2.3385193898335207E-4	0.0	0.0	14.19048643541828	0.0
19	2.3385193898335207E-4	0.0	0.0	15.010137481554928	0.0
20	2.3385193898335207E-4	0.0	0.0	15.73648160403722	0.0
21	2.3385193898335207E-4	0.0	0.0	16.67563099099436	0.0
22	2.3385193898335207E-4	0.0	0.0	17.662135395595634	0.0
23	2.3385193898335207E-4	0.0	0.0	18.581758145647665	0.0
24	2.3385193898335207E-4	0.0	0.0	19.27279062534347	0.0
25	2.3385193898335207E-4	0.0	0.0	19.861980585612027	0.0
26	2.3385193898335207E-4	0.0	0.0	20.434683984182254	0.0
27	2.3385193898335207E-4	0.0	0.0	21.151323251196736	0.0
28	2.3385193898335207E-4	0.0	0.0	21.754427401834803	0.0
29	2.3385193898335207E-4	0.0	0.0	22.478666856866244	0.0
30	2.3385193898335207E-4	0.0	0.0	23.29060078901644	0.0
31	2.3385193898335207E-4	0.0	0.0	23.886455529546023	0.0
32	2.3385193898335207E-4	0.0	0.0	24.47775015726543	0.0
33	2.3385193898335207E-4	0.0	0.0	25.074774157489927	0.0
34	2.3385193898335207E-4	0.0	0.0	25.781123939189143	0.0
35	2.3385193898335207E-4	0.0	0.0	26.53588107225791	0.0
36	2.3385193898335207E-4	0.0	0.0	27.16237041679431	0.0
37	2.3385193898335207E-4	0.0	0.0	27.81318036298498	0.0
38	2.3385193898335207E-4	0.0	0.0	28.66159519761658	0.0
39	2.3385193898335207E-4	0.0	0.0	30.43010048617818	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	30	2.1648466E-6	45.000004	30
GCGATAT	30	2.1648466E-6	45.000004	9
TTAATCG	20	7.032273E-4	45.0	20
ATAATCG	20	7.032273E-4	45.0	17
TAGCGGA	20	7.032273E-4	45.0	25
CACGATT	20	7.032273E-4	45.0	10
TAACGCA	20	7.032273E-4	45.0	19
TCGATAT	20	7.032273E-4	45.0	18
ATCGTGA	20	7.032273E-4	45.0	30
CGGTGTA	20	7.032273E-4	45.0	20
AACGACG	20	7.032273E-4	45.0	13
AATTCGG	20	7.032273E-4	45.0	13
CCGAATA	25	3.8899663E-5	45.0	26
CGATCGG	20	7.032273E-4	45.0	3
ACGGGCG	125	0.0	43.199997	5
TGCTACG	75	0.0	42.000004	1
TAATACG	125	0.0	41.399998	4
TTGTTCG	120	0.0	41.250004	1
ACGGGTA	50	1.0804797E-9	40.5	5
GGCGATA	50	1.0804797E-9	40.5	8
>>END_MODULE