##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934169.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1627815 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05852630673633 33.0 31.0 34.0 30.0 34.0 2 32.298863200056516 34.0 31.0 34.0 30.0 34.0 3 32.354590662943885 34.0 31.0 34.0 30.0 34.0 4 35.75023144521951 37.0 35.0 37.0 35.0 37.0 5 29.53116109631623 37.0 35.0 37.0 0.0 37.0 6 23.644825118333472 35.0 10.0 37.0 0.0 37.0 7 30.38648126476289 35.0 19.0 37.0 17.0 37.0 8 34.34799900480091 35.0 32.0 37.0 32.0 37.0 9 36.995585493437524 38.0 37.0 39.0 34.0 39.0 10 37.110432696590216 39.0 37.0 39.0 34.0 39.0 11 37.267795787604854 39.0 37.0 39.0 34.0 39.0 12 37.230743051268114 39.0 37.0 39.0 34.0 39.0 13 37.195789447818086 39.0 37.0 39.0 34.0 39.0 14 38.29352229829557 40.0 38.0 41.0 33.0 41.0 15 38.192050079400914 40.0 38.0 41.0 33.0 41.0 16 38.41571861667327 40.0 38.0 41.0 34.0 41.0 17 38.410545424387905 40.0 38.0 41.0 34.0 41.0 18 38.4649471838016 40.0 38.0 41.0 34.0 41.0 19 38.43704475017124 40.0 37.0 41.0 34.0 41.0 20 38.37174371780577 40.0 37.0 41.0 34.0 41.0 21 38.21377122093113 40.0 37.0 41.0 34.0 41.0 22 38.27882959672936 40.0 37.0 41.0 34.0 41.0 23 38.226069915807386 40.0 37.0 41.0 34.0 41.0 24 38.205941707135025 40.0 37.0 41.0 34.0 41.0 25 37.972576121979465 40.0 36.0 41.0 34.0 41.0 26 37.86381867718383 40.0 36.0 41.0 34.0 41.0 27 37.04272168520379 40.0 36.0 41.0 33.0 41.0 28 37.38202129848908 40.0 36.0 41.0 33.0 41.0 29 37.65744633143201 40.0 36.0 41.0 33.0 41.0 30 37.65467943224507 40.0 36.0 41.0 33.0 41.0 31 37.66814717888703 40.0 36.0 41.0 33.0 41.0 32 37.61386889787845 40.0 36.0 41.0 33.0 41.0 33 36.32604872175278 40.0 35.0 41.0 31.0 41.0 34 36.69820956312603 40.0 35.0 41.0 30.0 41.0 35 36.95015834108913 40.0 35.0 41.0 30.0 41.0 36 37.05356444067661 40.0 35.0 41.0 32.0 41.0 37 37.163606429477554 40.0 35.0 41.0 32.0 41.0 38 37.08083596723215 40.0 35.0 41.0 32.0 41.0 39 37.0782029898975 40.0 35.0 41.0 32.0 41.0 40 36.997032218034605 40.0 35.0 41.0 32.0 41.0 41 36.88341734165123 39.0 35.0 41.0 32.0 41.0 42 36.89269972324865 39.0 35.0 41.0 32.0 41.0 43 36.78954365207348 39.0 35.0 41.0 32.0 41.0 44 36.803649063314936 39.0 35.0 41.0 32.0 41.0 45 36.69927540906061 39.0 35.0 41.0 32.0 41.0 46 36.655775994200816 39.0 35.0 41.0 32.0 41.0 47 36.6073700021194 39.0 35.0 41.0 31.0 41.0 48 36.55340502452674 39.0 35.0 41.0 31.0 41.0 49 36.551946013521196 39.0 35.0 41.0 31.0 41.0 50 36.470345831682344 39.0 35.0 41.0 31.0 41.0 51 35.7844564646474 38.0 35.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 3.0 9 8.0 10 26.0 11 27.0 12 27.0 13 26.0 14 46.0 15 57.0 16 99.0 17 179.0 18 348.0 19 592.0 20 1015.0 21 1750.0 22 2889.0 23 4549.0 24 7387.0 25 10970.0 26 14266.0 27 15802.0 28 17037.0 29 19693.0 30 24705.0 31 33423.0 32 47748.0 33 77867.0 34 108723.0 35 134190.0 36 179807.0 37 276557.0 38 333456.0 39 314367.0 40 175.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.389380857161285 4.607710335633963 52.125517948906975 18.87739085829778 2 22.588561968036906 4.242128251674791 50.651578957068224 22.517730823220084 3 21.052945205689834 4.502047222810946 52.32504922242393 22.119958349075294 4 18.372726630483193 4.815166342612643 51.448229682119894 25.363877344784264 5 14.918587185890289 22.651591243476684 41.631635044522874 20.798186526110154 6 16.683775490458068 29.829003910149492 39.841013874426764 13.646206724965676 7 82.50108273974622 2.11191075152889 10.83102195274033 4.555984555984556 8 84.47858018263746 2.402484311792188 9.001882892097688 4.117052613472661 9 79.21962876616814 4.790102069338347 10.866959697508625 5.123309466984885 10 38.99822768557852 28.371405841572905 17.69046236826666 14.939904104581908 11 30.312289787230124 24.120984264182354 26.243645623120564 19.32308032546696 12 25.622321946904286 22.347072609602442 30.913586617643894 21.117018825849375 13 24.658146042394254 21.53100935917165 33.2347349053793 20.5761096930548 14 21.610686718085287 23.211605741438674 33.79364362657919 21.38406391389685 15 20.13029736180094 22.85572992016906 34.98431947119298 22.029653246837018 16 23.793182886261647 23.251475136916664 31.867933395379694 21.087408581441995 17 23.788084026747512 23.459914056572767 30.627313300344326 22.124688616335394 18 24.570052493680176 21.879943359656963 31.370641012645784 22.179363134017073 19 24.17952900053139 24.22314575059205 29.68046123177388 21.91686401710268 20 25.468188952675824 26.27749467844933 28.971781191351596 19.282535177523243 21 25.504986746036867 25.419780503312722 29.718794826193395 19.35643792445702 22 24.694268083289565 22.136483568464477 30.608515095388604 22.560733252857357 23 22.89676652445149 24.279233205247525 30.24182723466733 22.58217303563366 24 22.483206015425587 24.28119903060237 30.757917822356962 22.477677131615078 25 24.00635207317785 24.81215617253803 27.984383974837435 23.197107779446682 26 22.50667305559907 25.016417713315086 28.846336960895435 23.63057227019041 27 21.941006809741893 25.20126672871303 29.818621894994212 23.039104566550865 28 20.351145554009516 25.255572654140675 29.73151125895756 24.661770532892252 29 22.011469362304684 23.848287428239693 29.864695926748432 24.275547282707187 30 24.37629583214309 22.29927848066273 30.831329112952027 22.49309657424216 31 23.802029100358457 23.665711398408295 29.18458178601377 23.34767771521948 32 24.157106305077665 22.99290767071197 29.156691638791877 23.69329438541849 33 23.707239459029434 25.226945322410714 27.69559194380197 23.37022327475788 34 21.902796079407057 23.293371789791838 31.63959049400577 23.164241636795335 35 22.089672352202186 24.306017575707315 30.60396912425552 23.00034094783498 36 23.87697619201199 25.168093425849992 28.938177864192184 22.016752517945832 37 22.948553736143236 24.915239139582816 28.760946422044277 23.375260702229674 38 22.348362682491558 24.670862475158415 29.008763280839656 23.972011561510367 39 22.810454504965243 22.71154891679951 29.750063735743925 24.727932842491317 40 23.861925341638944 22.81119168947331 31.248636976560608 22.07824599232714 41 22.353154381794 23.288150066193026 30.058636884412543 24.30005866760043 42 22.882268562459494 23.515202894677838 30.421393094424122 23.18113544843855 43 22.22390136471282 22.5771970402042 30.589348298178848 24.60955329690413 44 22.996470729167626 22.80179258699545 29.51649911077119 24.685237573065734 45 22.43670195937499 21.92687744000393 29.12646707396111 26.50995352665997 46 22.772366638715088 22.73851758338632 29.63795025847532 24.851165519423276 47 20.792104753918597 24.110356520857714 31.3889477612628 23.708590963960894 48 21.25130927040235 23.91444973783876 30.436075352543135 24.398165639215758 49 22.895292155435353 22.39523533079619 30.723577310689482 23.985895203078975 50 21.751181798914494 22.412866326947473 30.18623123635057 25.649720637787464 51 21.124022078676017 22.416122225191437 29.414767648657865 27.04508804747468 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 347.0 1 997.0 2 1647.0 3 9245.5 4 16844.0 5 11481.5 6 6119.0 7 5930.5 8 5742.0 9 5779.5 10 5817.0 11 5732.5 12 5648.0 13 5596.0 14 5544.0 15 5218.0 16 4892.0 17 4910.5 18 4929.0 19 4867.5 20 4806.0 21 5353.0 22 5900.0 23 6534.5 24 7169.0 25 8356.0 26 11875.0 27 14207.0 28 16129.5 29 18052.0 30 21455.5 31 24859.0 32 28627.5 33 32396.0 34 36756.5 35 41117.0 36 45346.5 37 49576.0 38 52660.5 39 55745.0 40 61089.0 41 66433.0 42 73032.5 43 79632.0 44 89346.0 45 99060.0 46 115580.5 47 132101.0 48 143863.0 49 155625.0 50 154496.5 51 153368.0 52 139831.0 53 126294.0 54 115820.0 55 105346.0 56 98376.0 57 91406.0 58 83890.5 59 76375.0 60 68619.5 61 60864.0 62 56082.0 63 51300.0 64 44699.0 65 38098.0 66 31897.0 67 25696.0 68 21432.5 69 17169.0 70 14136.0 71 11103.0 72 9196.5 73 7290.0 74 6224.0 75 4030.5 76 2903.0 77 1954.0 78 1005.0 79 740.5 80 476.0 81 319.5 82 163.0 83 93.0 84 23.0 85 23.0 86 23.0 87 12.5 88 2.0 89 2.5 90 3.0 91 1.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1627815.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.18674824328572 #Duplication Level Percentage of deduplicated Percentage of total 1 72.83105409473207 27.811811270086572 2 8.89464989925423 6.793155128299759 3 3.659228264121116 4.192020854601254 4 2.301989003811759 3.5162189818948697 5 1.8140100520465448 3.463557258414552 6 1.4577362470726842 3.3399724243246047 7 1.24084722005919 3.316874428054687 8 1.0481126048758864 3.2019209738407897 9 0.8838811096328423 3.0377290869490836 >10 5.767168406403254 35.366722022980525 >50 0.06483571059351281 1.6881321293879723 >100 0.03237087241799415 2.2823516385717126 >500 0.0022453718066280035 0.592415772062378 >1k 0.0016840288549710026 1.0007689426493387 >5k 1.8711431721900028E-4 0.3963490878820421 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5547 0.340763538854231 No Hit CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2496 0.15333437767805308 No Hit CTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGCT 2258 0.13871355160137977 Illumina Single End Adapter 1 (95% over 21bp) CCTGTCTCTTATACACATCTGACGCTGCCTTGTTCGTATGCCGTCTTCTGC 2047 0.12575139066785845 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.14786692590988534 0.0 2 0.0 0.0 0.0 0.44826961294741724 0.0 3 0.0 0.0 0.0 0.6904347238476117 0.0 4 0.0 0.0 0.0 0.9635001520443048 0.0 5 0.0 0.0 0.0 1.512825474639317 0.0 6 0.0 0.0 0.0 2.22629721436404 0.0 7 0.0 0.0 0.0 3.005439807349115 0.0 8 0.0 0.0 0.0 4.097640088093549 0.0 9 0.0 0.0 0.0 4.757113062602323 0.0 10 0.0 0.0 0.0 5.708203942094157 0.0 11 0.0 0.0 0.0 7.06597494186993 0.0 12 0.0 0.0 0.0 8.182318015253577 0.0 13 0.0 0.0 0.0 8.592622626035514 0.0 14 0.0 0.0 0.0 8.793443972441585 0.0 15 0.0 0.0 0.0 9.023568403043344 0.0 16 0.0 0.0 0.0 9.445606533912024 0.0 17 0.0 0.0 0.0 10.012931444912352 0.0 18 0.0 0.0 0.0 10.603354803832131 0.0 19 0.0 0.0 0.0 11.045542644587991 0.0 20 0.0 0.0 0.0 11.494426577958798 0.0 21 0.0 0.0 0.0 12.119866201011785 0.0 22 0.0 0.0 0.0 12.781489297002423 0.0 23 0.0 0.0 0.0 13.388437875311384 0.0 24 0.0 0.0 0.0 13.902685501730847 0.0 25 0.0 0.0 0.0 14.3571597509545 0.0 26 0.0 0.0 0.0 14.76328698285739 0.0 27 0.0 0.0 0.0 15.20479907114752 0.0 28 0.0 0.0 0.0 15.626652905889182 0.0 29 6.143204233896358E-5 0.0 0.0 16.11767922030452 0.0 30 6.143204233896358E-5 0.0 0.0 16.652383716822857 0.0 31 6.143204233896358E-5 0.0 0.0 17.110298160417493 0.0 32 6.143204233896358E-5 0.0 0.0 17.573127167399242 0.0 33 6.143204233896358E-5 0.0 0.0 18.02262542119344 0.0 34 6.143204233896358E-5 0.0 0.0 18.482137097888888 0.0 35 6.143204233896358E-5 0.0 0.0 18.991347296836558 0.0 36 6.143204233896358E-5 0.0 0.0 19.445452953806175 0.0 37 6.143204233896358E-5 0.0 0.0 19.914302300937145 0.0 38 6.143204233896358E-5 0.0 0.0 20.471982381290257 0.0 39 6.143204233896358E-5 0.0 0.0 21.268325946130243 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTAT 1640 0.0 43.90244 1 CGTTATT 1405 0.0 41.797153 1 CGTTTTT 3425 0.0 41.51825 1 TTTACGG 950 0.0 40.5 2 TAGGGCG 785 0.0 40.127388 5 TTACGGG 1605 0.0 39.672894 3 TTATGGG 4405 0.0 39.585697 3 TATGGGA 2780 0.0 39.57734 4 TTAGGGA 3470 0.0 39.488472 4 GTTTACG 155 0.0 39.19355 1 TAGGGAT 1695 0.0 38.893806 5 CGTTAGG 570 0.0 38.68421 2 TAGGGAG 2210 0.0 38.178734 5 CGTAAGG 65 9.094947E-12 38.076927 2 CTAGGGA 2060 0.0 37.900486 4 GTACGGG 600 0.0 37.875 3 CACGACC 1060 0.0 37.78302 27 TACGGGT 245 0.0 37.65306 4 TATGGGG 3840 0.0 37.617184 4 AACACGT 1035 0.0 37.608696 41 >>END_MODULE