FastQCFastQC Report
Sat 14 Jan 2017
SRR2934168.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934168.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620491
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC50130.8079085756280108No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG42030.6773667950058906No Hit
GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC28340.45673506948529474No Hit
CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC26300.42385788029157556TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT25490.4108037022293635TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC20960.33779700269625185TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCTCTAT19090.30765957926867593No Hit
TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC18010.2902540085190599TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTC12030.19387871862766745No Hit
GAATCAGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC11590.18678756017412018No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT11410.18388663171585085No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTA9380.15117060521425774No Hit
CCTGTATCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC9240.14891432752449268TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC9170.1477861886796102TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCACTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT8700.14021154214968468TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC8050.12973596716148986TruSeq Adapter, Index 13 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG6950.11200807102762168No Hit
GAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT6930.11168574564336953No Hit
GAATATGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC6880.11087993218273914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAC351.2107193E-745.039
ACTATCG453.8380676E-1045.033
CGAACGG207.0307753E-445.012
GCAAACG253.888723E-545.034
CGGATCT253.888723E-545.010
ACTCGCA207.0307753E-445.012
CGTCGTT207.0307753E-445.035
CGTCGAG207.0307753E-445.026
GCGGATC253.888723E-545.09
ATAACGG207.0307753E-445.018
CCCGGTA207.0307753E-445.017
GATTACC207.0307753E-445.09
TTCGAAC551.8189894E-1245.028
ATACCCG207.0307753E-445.018
TCGAACA551.8189894E-1245.029
ATCGAAT453.8380676E-1045.043
AGGTCCG207.0307753E-445.014
TTAGGCG302.1638807E-644.9999964
GATCGGT302.1638807E-644.9999969
GTTTACG302.1638807E-644.9999961
Produced by FastQC (version 0.11.5)