##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934168.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 620491 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.888356156656585 33.0 31.0 34.0 30.0 34.0 2 32.05889368258363 33.0 31.0 34.0 30.0 34.0 3 32.15478064951788 34.0 31.0 34.0 30.0 34.0 4 35.64522934256903 37.0 35.0 37.0 33.0 37.0 5 29.467553921007717 37.0 35.0 37.0 0.0 37.0 6 23.62058112043527 35.0 10.0 37.0 0.0 37.0 7 30.35865628993813 35.0 19.0 37.0 17.0 37.0 8 34.11625631959207 35.0 32.0 37.0 32.0 37.0 9 36.88540526776376 38.0 37.0 39.0 34.0 39.0 10 36.91845490103805 39.0 37.0 39.0 33.0 39.0 11 37.13953949372352 39.0 37.0 39.0 34.0 39.0 12 37.16143666870269 39.0 37.0 39.0 34.0 39.0 13 37.07708733889774 39.0 37.0 39.0 33.0 39.0 14 38.223769563136294 40.0 38.0 41.0 33.0 41.0 15 38.062545629187206 40.0 37.0 41.0 33.0 41.0 16 38.309201906232325 40.0 37.0 41.0 34.0 41.0 17 38.271775094239885 40.0 37.0 41.0 34.0 41.0 18 38.36028725638244 40.0 37.0 41.0 34.0 41.0 19 38.373238290321694 40.0 37.0 41.0 34.0 41.0 20 38.25811494445528 40.0 37.0 41.0 34.0 41.0 21 38.19563378034492 40.0 37.0 41.0 34.0 41.0 22 38.27155752460551 40.0 37.0 41.0 34.0 41.0 23 38.174542418826384 40.0 37.0 41.0 34.0 41.0 24 38.14506092755576 40.0 37.0 41.0 34.0 41.0 25 37.91199872359148 40.0 37.0 41.0 33.0 41.0 26 37.7796486975637 40.0 37.0 41.0 33.0 41.0 27 36.927421993227945 40.0 36.0 41.0 32.0 41.0 28 37.32102157807285 40.0 36.0 41.0 32.0 41.0 29 37.63043138417802 40.0 36.0 41.0 32.0 41.0 30 37.67338124162961 40.0 36.0 41.0 33.0 41.0 31 37.7615162830726 40.0 36.0 41.0 33.0 41.0 32 37.667516531263146 40.0 36.0 41.0 33.0 41.0 33 36.49001033052856 40.0 36.0 41.0 31.0 41.0 34 36.97909558720433 40.0 36.0 41.0 31.0 41.0 35 37.30288432870098 40.0 36.0 41.0 31.0 41.0 36 37.42520520039775 40.0 36.0 41.0 33.0 41.0 37 37.58504797007531 40.0 36.0 41.0 33.0 41.0 38 37.54022056726044 40.0 36.0 41.0 33.0 41.0 39 37.5836523011615 40.0 36.0 41.0 33.0 41.0 40 37.51834112017741 40.0 36.0 41.0 33.0 41.0 41 37.42659925768464 40.0 36.0 41.0 33.0 41.0 42 37.463511960689196 40.0 36.0 41.0 33.0 41.0 43 37.34528784462627 40.0 36.0 41.0 33.0 41.0 44 37.411786794651334 40.0 36.0 41.0 33.0 41.0 45 37.365052837188614 39.0 36.0 41.0 33.0 41.0 46 37.31973549978968 39.0 35.0 41.0 33.0 41.0 47 37.21475895701952 39.0 35.0 41.0 33.0 41.0 48 37.19809956953445 39.0 35.0 41.0 33.0 41.0 49 37.266785497291664 39.0 35.0 41.0 33.0 41.0 50 37.193927067435304 39.0 35.0 41.0 33.0 41.0 51 36.47713020817385 38.0 35.0 40.0 31.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 10.0 11 4.0 12 8.0 13 9.0 14 7.0 15 10.0 16 24.0 17 37.0 18 68.0 19 147.0 20 246.0 21 519.0 22 854.0 23 1350.0 24 1840.0 25 2528.0 26 3139.0 27 3770.0 28 4833.0 29 6490.0 30 9205.0 31 13018.0 32 19070.0 33 30292.0 34 41878.0 35 55939.0 36 72223.0 37 109683.0 38 129605.0 39 113614.0 40 68.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.399415946403735 4.253566933283481 52.276664770318995 17.070352349993794 2 18.429759658077234 8.727443266703304 51.91727196687784 20.92552510834162 3 19.534046424525094 8.713583275180461 50.61878415641806 21.133586143876382 4 18.02427432468803 4.12012422420309 54.88540526776375 22.970196183345127 5 16.902581987490553 21.858173607675212 41.20043642857028 20.038807976263957 6 15.155900730228158 31.782733351491 39.34271407643302 13.718651841847826 7 79.39792841475541 1.5360416186536145 13.764260883719507 5.301769082871468 8 80.05950126593294 5.035044827402815 8.345165360980255 6.560288545683983 9 75.89715241639283 3.6232596443784035 10.512320081999578 9.967267857229194 10 36.19246693344464 25.264024780375543 19.633644968259006 18.90986331792081 11 28.05358981838576 25.849367678177444 25.25709478461412 20.839947718822675 12 22.46785207198815 22.002253054435922 31.194973013307205 24.334921860268725 13 21.068315253565324 24.491894322399517 34.05190405662613 20.387886367409035 14 19.09729552886343 26.91690935082056 29.89712985361593 24.088665266700083 15 16.475017365280074 24.00453834141027 36.02147331709888 23.498970976210774 16 18.147724946856602 24.539437316576713 30.798835116061312 26.514002620505373 17 18.503894496455224 25.364590300262215 29.90470450014585 26.22681070313671 18 18.816711281871935 23.254809497639773 32.88347453871208 25.045004681776206 19 20.176763240723876 24.644031903766532 30.196892460970425 24.982312394539164 20 22.17034574232342 25.953156451906633 31.211733933288315 20.664763872481632 21 21.94197820758077 27.48436318979647 29.615256305087424 20.95840229753534 22 20.602555073320968 23.995835556035463 29.65441883927406 25.74719053136951 23 20.31133408864915 26.441640571740766 28.397672166074933 24.849353173535153 24 21.55196449263567 24.380692064832527 28.733212891081415 25.334130551450386 25 18.588988397897793 28.13916720790471 27.185245233210477 26.086599160987024 26 18.439590582296923 26.978312336520595 28.366084278418217 26.21601280276426 27 19.663298903610205 27.297575629622344 26.878874955478803 26.16025051128864 28 15.817473581405695 24.828402023558763 31.510207239105803 27.843917155929738 29 17.686477321991777 23.567142794980104 29.910345194370265 28.836034688657854 30 19.03137998778387 25.621322468819045 28.927252772401214 26.420044770995872 31 20.387886367409035 24.607931460730292 25.264347105759793 29.739835066100877 32 20.01463357244505 27.412162303723985 26.534309119713264 26.038895004117705 33 18.704542048152188 26.3194792511092 26.603609077327473 28.372369623411135 34 17.971574124362803 25.18892296584479 27.899357122021108 28.9401457877713 35 17.446022585339673 23.84111937159443 28.66133433039319 30.051523712672708 36 19.148061776883146 26.71158808105194 27.31997724382787 26.820372898237043 37 17.115639066481222 23.901071893065332 29.466664302947184 29.516624737506262 38 17.124986502624534 25.32236567492518 28.095169792954287 29.457478029496 39 19.093105298868153 24.570058228080665 29.28358348469196 27.053252988359215 40 19.11212249653903 22.57824851609451 31.949697900533607 26.359931086832848 41 17.541108573694057 24.922359873068263 26.814893366704755 30.721638186532925 42 18.777387584993175 24.029035070613432 29.42653479260779 27.767042551785604 43 19.999645442077323 25.26418594306767 26.895313550075667 27.840855064779348 44 19.27473565289424 22.683326591360714 28.213785534359083 29.828152221385967 45 18.581574914059996 22.28557706719356 27.559626167019346 31.5732218517271 46 21.752289718948383 23.302835979893345 28.508713260949797 26.43616104020848 47 16.02746856924597 23.326204570251623 33.379210979691884 27.267115880810522 48 17.248437124793107 23.501227253900538 30.066995331116807 29.18334029018954 49 18.86844450604441 20.838980742669918 33.54585320335025 26.746721547935426 50 19.521959222615635 20.55646254337291 29.987542123898653 29.9340361101128 51 18.089222889614838 20.854774686498274 28.325148954618196 32.730853469268695 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 92.0 1 310.5 2 529.0 3 987.0 4 1445.0 5 1076.0 6 707.0 7 739.0 8 771.0 9 865.5 10 960.0 11 1038.5 12 1117.0 13 1189.5 14 1262.0 15 1151.0 16 1040.0 17 1122.5 18 1205.0 19 1252.5 20 1300.0 21 1618.0 22 1936.0 23 2226.5 24 2517.0 25 2662.0 26 3890.5 27 4974.0 28 6110.0 29 7246.0 30 8241.0 31 9236.0 32 10315.5 33 11395.0 34 12119.5 35 12844.0 36 14342.5 37 15841.0 38 18319.0 39 20797.0 40 24470.0 41 28143.0 42 32597.5 43 37052.0 44 42927.5 45 48803.0 46 65407.0 47 82011.0 48 80993.5 49 79976.0 50 74050.5 51 68125.0 52 57434.5 53 46744.0 54 40241.5 55 33739.0 56 30121.5 57 26504.0 58 23419.5 59 20335.0 60 17609.5 61 14884.0 62 13347.0 63 11810.0 64 10051.5 65 8293.0 66 6830.0 67 5367.0 68 4452.5 69 3538.0 70 2856.5 71 2175.0 72 1766.0 73 1357.0 74 1096.0 75 617.5 76 400.0 77 320.5 78 241.0 79 169.0 80 97.0 81 61.0 82 25.0 83 18.5 84 12.0 85 7.0 86 2.0 87 1.5 88 1.0 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 620491.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.222616149599915 #Duplication Level Percentage of deduplicated Percentage of total 1 73.28395264234739 28.743883444162492 2 10.999900047951414 8.62889714529528 3 4.02867935142675 4.740460313724918 4 2.1570749112842047 3.3842448500493054 5 1.487176409803632 2.916547473423398 6 1.1499650981271667 2.7062783777567314 7 0.9018034690942034 2.475976391746167 8 0.7278036305227273 2.2837089945822537 9 0.6092056275706302 2.1505174637741056 >10 4.516951382470893 30.979496192516603 >50 0.08665874341375221 2.2866328805677556 >100 0.04166285736660017 3.027709138577646 >500 0.004582914310326019 1.339003059234954 >1k 0.004166285736660017 3.517456779354697 >5k 4.1662857366600174E-4 0.8191874952336862 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGC 5013 0.8079085756280108 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCG 4203 0.6773667950058906 No Hit GAATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC 2834 0.45673506948529474 No Hit CCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC 2630 0.42385788029157556 TruSeq Adapter, Index 16 (95% over 21bp) CTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT 2549 0.4108037022293635 TruSeq Adapter, Index 13 (95% over 22bp) GCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC 2096 0.33779700269625185 TruSeq Adapter, Index 16 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCCTCTAT 1909 0.30765957926867593 No Hit TCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC 1801 0.2902540085190599 TruSeq Adapter, Index 16 (95% over 21bp) GAATGATCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTC 1203 0.19387871862766745 No Hit GAATCAGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC 1159 0.18678756017412018 No Hit GAATGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCT 1141 0.18388663171585085 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCCTCTATCGTA 938 0.15117060521425774 No Hit CCTGTATCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC 924 0.14891432752449268 TruSeq Adapter, Index 13 (95% over 21bp) GCTGTATCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC 917 0.1477861886796102 TruSeq Adapter, Index 13 (95% over 21bp) CTGTCACTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGCT 870 0.14021154214968468 TruSeq Adapter, Index 13 (95% over 22bp) TCTGTATCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTGC 805 0.12973596716148986 TruSeq Adapter, Index 13 (95% over 21bp) TCCTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCTG 695 0.11200807102762168 No Hit GAACTGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTCT 693 0.11168574564336953 No Hit GAATATGTCTCTTATACACATCTGACGCACCCTCTATCGTATGCCGTCTTC 688 0.11087993218273914 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.6116269212607435E-4 0.0 0.0 0.4349781060482747 0.0 2 1.6116269212607435E-4 0.0 0.0 1.577943918606394 0.0 3 1.6116269212607435E-4 0.0 0.0 2.150877289114588 0.0 4 1.6116269212607435E-4 0.0 0.0 2.8532243014000205 0.0 5 1.6116269212607435E-4 0.0 0.0 4.258562976739388 0.0 6 1.6116269212607435E-4 0.0 0.0 4.97493114323979 0.0 7 1.6116269212607435E-4 0.0 0.0 6.067936521238825 0.0 8 1.6116269212607435E-4 0.0 0.0 7.690522505564142 0.0 9 1.6116269212607435E-4 0.0 0.0 8.288113767967626 0.0 10 1.6116269212607435E-4 0.0 0.0 10.324887871056953 0.0 11 1.6116269212607435E-4 0.0 0.0 12.498327937069192 0.0 12 1.6116269212607435E-4 0.0 0.0 15.319319700043998 0.0 13 1.6116269212607435E-4 0.0 0.0 16.036977168081407 0.0 14 1.6116269212607435E-4 0.0 0.0 16.2990277054784 0.0 15 1.6116269212607435E-4 0.0 0.0 16.82474040719366 0.0 16 1.6116269212607435E-4 0.0 0.0 17.698886849285486 0.0 17 1.6116269212607435E-4 0.0 0.0 18.89455286216883 0.0 18 1.6116269212607435E-4 0.0 0.0 20.096504220045095 0.0 19 1.6116269212607435E-4 0.0 0.0 21.302645807916633 0.0 20 1.6116269212607435E-4 0.0 0.0 22.22127315303526 0.0 21 1.6116269212607435E-4 0.0 0.0 23.469155878167452 0.0 22 1.6116269212607435E-4 0.0 0.0 24.62984958685944 0.0 23 1.6116269212607435E-4 0.0 0.0 25.718825897555323 0.0 24 1.6116269212607435E-4 0.0 0.0 26.59168303811014 0.0 25 1.6116269212607435E-4 0.0 0.0 27.3541437345586 0.0 26 1.6116269212607435E-4 0.0 0.0 28.052300516848753 0.0 27 1.6116269212607435E-4 0.0 0.0 28.73224591492866 0.0 28 1.6116269212607435E-4 0.0 0.0 29.43346478836921 0.0 29 1.6116269212607435E-4 0.0 0.0 30.208012686727123 0.0 30 1.6116269212607435E-4 0.0 0.0 30.99770987814489 0.0 31 1.6116269212607435E-4 0.0 0.0 31.742281515767353 0.0 32 1.6116269212607435E-4 0.0 0.0 32.395957395030706 0.0 33 1.6116269212607435E-4 0.0 0.0 33.060108849282265 0.0 34 1.6116269212607435E-4 0.0 0.0 33.743922151973194 0.0 35 1.6116269212607435E-4 0.0 0.0 34.43821102965232 0.0 36 1.6116269212607435E-4 0.0 0.0 35.09962271813773 0.0 37 1.6116269212607435E-4 0.0 0.0 35.7131690870617 0.0 38 1.6116269212607435E-4 0.0 0.0 36.352501486725835 0.0 39 1.6116269212607435E-4 0.0 0.0 37.07628313706403 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCATAC 35 1.2107193E-7 45.0 39 ACTATCG 45 3.8380676E-10 45.0 33 CGAACGG 20 7.0307753E-4 45.0 12 GCAAACG 25 3.888723E-5 45.0 34 CGGATCT 25 3.888723E-5 45.0 10 ACTCGCA 20 7.0307753E-4 45.0 12 CGTCGTT 20 7.0307753E-4 45.0 35 CGTCGAG 20 7.0307753E-4 45.0 26 GCGGATC 25 3.888723E-5 45.0 9 ATAACGG 20 7.0307753E-4 45.0 18 CCCGGTA 20 7.0307753E-4 45.0 17 GATTACC 20 7.0307753E-4 45.0 9 TTCGAAC 55 1.8189894E-12 45.0 28 ATACCCG 20 7.0307753E-4 45.0 18 TCGAACA 55 1.8189894E-12 45.0 29 ATCGAAT 45 3.8380676E-10 45.0 43 AGGTCCG 20 7.0307753E-4 45.0 14 TTAGGCG 30 2.1638807E-6 44.999996 4 GATCGGT 30 2.1638807E-6 44.999996 9 GTTTACG 30 2.1638807E-6 44.999996 1 >>END_MODULE