FastQCFastQC Report
Sat 14 Jan 2017
SRR2934167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569162
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC79741.4010070946408932No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG67901.1929819629560654No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC45510.7995965999135571No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCATGTTT26680.468759333897906No Hit
GCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC21720.3816136706245322TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC21510.3779240356875547TruSeq Adapter, Index 14 (95% over 21bp)
GAATCAGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC16760.2944680073511584No Hit
CTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGCT15840.27830389238916164Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTC15320.26916765349759825No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCT15020.2638967464447732No Hit
TCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC13820.24281311823347307TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTA12050.2117147666218054No Hit
GAATATGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTC9630.16919611639568347No Hit
GCTGTATCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC9070.15935708989707675No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGC9000.15812721158475093No Hit
GAACTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCT8220.14442285324740584No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCCATGTTTCGTATGCCG7490.13159697941886492No Hit
CCTGTATCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC6610.11613565206391151No Hit
GCTGACTCTTATACACATCTGACGCCCCATGTTTCGTATGCCGTCTTCTGC5970.10489105035121811TruSeq Adapter, Index 14 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG1250.045.0000041
CGAGATA551.8189894E-1245.00000419
CCGGGTA502.1827873E-1145.05
CGAACCC207.030283E-445.034
CAACCCG253.8883147E-545.023
CGCGCAT253.8883147E-545.036
ATCCGGC700.045.06
TACGCGC253.8883147E-545.034
TCTCGGC207.030283E-445.015
GTCGTTA207.030283E-445.09
TCGTCCC207.030283E-445.038
GACGGCT207.030283E-445.015
AGCGAAA351.2104829E-745.022
TCATCGG253.8883147E-545.044
TTAGTCG253.8883147E-545.013
CTCGAAT207.030283E-445.043
AGTACGG207.030283E-445.03
ACGCGCA253.8883147E-545.035
CGACGTT302.1635642E-644.99999627
TCCGATG302.1635642E-644.9999961
Produced by FastQC (version 0.11.5)