SRR2934165.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934165.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	801582
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	13348	1.6652070530525884	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	12260	1.5294754622733544	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	8291	1.034329613189917	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT	5609	0.6997412616550771	No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2988	0.37276286144149945	Illumina PCR Primer Index 3 (95% over 22bp)
GAATCAGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	2980	0.37176483503871094	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC	2913	0.36340636391535736	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT	2877	0.3589152451028092	No Hit
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2503	0.3122575107724475	RNA PCR Primer, Index 7 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTA	2229	0.27807510647694184	No Hit
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	1940	0.2420214026762078	TruSeq Adapter, Index 7 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT	1666	0.20783899838070213	TruSeq Adapter, Index 7 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	1643	0.20496967247268524	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	1276	0.15918521124476348	No Hit
GCTGTATCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1268	0.158187184841975	TruSeq Adapter, Index 7 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1201	0.14982871371862141	Illumina PCR Primer Index 3 (95% over 22bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCTCGAATGC	1181	0.14733364771165022	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	1136	0.14171974919596497	No Hit
CCTGTATCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1049	0.1308662120656402	TruSeq Adapter, Index 7 (95% over 23bp)
CTGTCACTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	847	0.10566604539523095	TruSeq Adapter, Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	808	0.10080066668163706	No Hit

Adapter Content

Adapter graph

Kmer Content

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	35	1.2113196E-7	45.000004	30
ACGACCG	25	3.8897448E-5	45.000004	38
TACGATA	20	7.032009E-4	45.0	25
CTACGAT	20	7.032009E-4	45.0	24
CGTACAG	20	7.032009E-4	45.0	30
CGTAAGC	55	1.8189894E-12	45.0	43
CTTTACG	150	0.0	43.500004	1
GCGAACC	100	0.0	42.750004	33
CTCGAAT	390	0.0	42.692307	43
ATCAGTC	380	0.0	42.63158	3
ACGGGCG	95	0.0	42.63158	5
CGATGAA	2755	0.0	42.63158	19
CCGATGA	2720	0.0	42.6011	18
ATGAATG	2890	0.0	42.430794	21
TCTACGG	175	0.0	42.428574	2
TCGTTAG	85	0.0	42.35294	1
TACGGCT	1865	0.0	42.345844	7
TAATACG	630	0.0	42.142857	4
AATACGG	625	0.0	42.120003	5
GATGAAT	2960	0.0	42.035473	20

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content