##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934165.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	801582
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.845320877963825	31.0	31.0	34.0	30.0	34.0
2	31.934250020584294	33.0	31.0	34.0	30.0	34.0
3	32.044089063876186	33.0	31.0	34.0	30.0	34.0
4	35.653769670476635	37.0	35.0	37.0	35.0	37.0
5	29.63567669932708	37.0	35.0	37.0	0.0	37.0
6	23.81574062291818	35.0	17.0	37.0	0.0	37.0
7	30.41712887764446	35.0	19.0	37.0	17.0	37.0
8	33.76176361245637	35.0	32.0	37.0	29.0	37.0
9	36.68167823129761	37.0	35.0	39.0	33.0	39.0
10	36.91769525762804	39.0	37.0	39.0	34.0	39.0
11	37.05739649842437	39.0	37.0	39.0	34.0	39.0
12	37.083362650358914	39.0	37.0	39.0	34.0	39.0
13	37.00531948072686	39.0	37.0	39.0	33.0	39.0
14	38.2029698770681	40.0	37.0	41.0	33.0	41.0
15	37.973613429443276	40.0	37.0	41.0	33.0	41.0
16	38.28306024835887	40.0	37.0	41.0	34.0	41.0
17	38.12131260432495	40.0	37.0	41.0	33.0	41.0
18	38.24132278419425	40.0	37.0	41.0	34.0	41.0
19	38.23655970318695	40.0	37.0	41.0	34.0	41.0
20	37.91260407544082	40.0	37.0	41.0	33.0	41.0
21	38.04264442065815	40.0	37.0	41.0	33.0	41.0
22	38.10763839507374	40.0	37.0	41.0	34.0	41.0
23	37.84562777108268	40.0	37.0	41.0	33.0	41.0
24	37.91909249459194	40.0	37.0	41.0	33.0	41.0
25	37.85791846623302	40.0	36.0	41.0	33.0	41.0
26	37.743379966117004	40.0	36.0	41.0	33.0	41.0
27	36.6970291249055	40.0	36.0	41.0	31.0	41.0
28	37.10927890097332	40.0	36.0	41.0	31.0	41.0
29	37.452723489299906	40.0	36.0	41.0	32.0	41.0
30	37.44572233408435	40.0	36.0	41.0	32.0	41.0
31	37.5353975014409	40.0	36.0	41.0	33.0	41.0
32	37.23328742411881	40.0	36.0	41.0	32.0	41.0
33	36.04662779353828	39.0	35.0	41.0	29.0	41.0
34	36.38115252088994	40.0	35.0	41.0	29.0	41.0
35	36.61251624911737	40.0	35.0	41.0	30.0	41.0
36	36.7138496123915	39.0	35.0	41.0	31.0	41.0
37	36.681915262568275	39.0	35.0	41.0	31.0	41.0
38	36.82045505013835	40.0	35.0	41.0	31.0	41.0
39	36.73861563757669	39.0	35.0	41.0	31.0	41.0
40	36.956108046338365	39.0	35.0	41.0	31.0	41.0
41	36.73728202479597	39.0	35.0	41.0	30.0	41.0
42	36.72885244429141	39.0	35.0	41.0	31.0	41.0
43	36.571563483211946	39.0	35.0	41.0	31.0	41.0
44	36.66221297384422	39.0	35.0	41.0	31.0	41.0
45	36.6598439086706	39.0	35.0	41.0	31.0	41.0
46	36.52305316237141	39.0	35.0	41.0	31.0	41.0
47	36.41286231477254	39.0	35.0	41.0	31.0	41.0
48	36.40038199460567	39.0	35.0	41.0	31.0	41.0
49	36.48540136879321	39.0	35.0	41.0	31.0	41.0
50	36.359156018972485	39.0	35.0	41.0	31.0	41.0
51	35.774841500931906	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	11.0
11	9.0
12	7.0
13	13.0
14	13.0
15	12.0
16	16.0
17	64.0
18	120.0
19	375.0
20	1061.0
21	2499.0
22	4470.0
23	6226.0
24	7405.0
25	7394.0
26	6666.0
27	6061.0
28	6588.0
29	8231.0
30	11393.0
31	16278.0
32	23678.0
33	37544.0
34	52064.0
35	68810.0
36	88979.0
37	133565.0
38	162703.0
39	149201.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.109877716815	3.8553759939719203	47.224862833746265	16.809883455466814
2	17.164557088357775	16.627494130357217	47.2832473783094	18.924701402975614
3	18.348341155365265	16.602044457086112	45.6832863013391	19.36632808620952
4	16.58407998183592	3.841653130933579	57.31740483194483	22.256862055285673
5	22.534812408462265	21.150674541094986	36.99858529757405	19.315927752868703
6	14.533260477405928	36.51641379172686	35.80569423964111	13.1446314912261
7	74.68967616538296	1.5001584366914427	19.112704626600895	4.697460771324705
8	74.92620842284383	11.387980269018016	8.147762799064848	5.538048509073308
9	70.8118445773483	4.1592750336210145	9.229249159786521	15.79963122924417
10	42.53875461275328	26.7051904858143	16.133096801075872	14.622958100356545
11	31.66188861526332	22.14009296615942	26.46815921515204	19.729859203425228
12	22.56575122694871	19.82130337258072	30.08849999126727	27.524445409203302
13	25.024389270218144	21.572964462774863	34.37876599025427	19.02388027675272
14	19.923102065665148	29.287708556329857	28.659450935774505	22.12973844223049
15	16.635478341579528	22.356165682363127	36.12905479414458	24.879301181912766
16	18.765141931829806	23.29817785329511	27.88860029292075	30.048079921954336
17	18.643881723891006	27.36251562535087	30.504801754530416	23.48880089622771
18	18.767387491236082	22.378746029726216	31.380445169676964	27.47342130936074
19	20.056962356939152	24.50304522806151	27.654313594866153	27.785678820133185
20	25.63343488251982	24.651501655476295	31.24122048648797	18.473842975515918
21	22.771469419223486	31.29374162593471	27.206823506515864	18.727965448325936
22	21.152296333999516	21.475781641803334	32.52293589426908	24.848986129928065
23	23.506765371477904	26.07356452614954	27.694733664179083	22.72493643819347
24	21.488381725138538	25.914504068205126	27.91529749919534	24.681816707460996
25	18.525865101761266	32.30399385215736	25.398898677864523	23.771242368216853
26	19.07078751768378	24.062042311329346	32.18435543712309	24.682814733863783
27	23.399727039778835	27.958212634515245	25.386548101130014	23.2555122245759
28	17.872407314535508	27.22965336047965	31.32567847082395	23.5722608541609
29	19.644403192686465	21.294764602997574	34.62203492593397	24.438797278381998
30	19.163104959941716	31.016040779358818	28.446621805379863	21.374232455319607
31	23.800434640498413	24.461252872444742	24.934067880765788	26.80424460629106
32	24.94579469099855	26.172743399926645	26.490739562515124	22.390722346559677
33	22.72431267169173	25.155005975683085	23.107679563662856	29.013001788962328
34	21.10863267887752	24.43779925197921	27.942743225272025	26.510824843871244
35	20.794379115299495	24.419335763527624	27.12560910798895	27.660676013183927
36	21.153793373603698	27.460197459523794	26.616865149167523	24.76914401770499
37	19.10896202759044	26.923883021325327	29.96786354983021	23.99929140125402
38	21.840061278821132	23.967479309665137	24.78873028585971	29.40372912565402
39	20.253573558288483	27.29103198425114	28.790067641239446	23.665326816220922
40	18.862449506101683	22.908947556207597	27.254604020549362	30.973998917141355
41	18.882909047358847	26.797882187973283	23.598459047234094	30.720749717433776
42	19.763542594519336	21.75086266907191	28.1440950520346	30.341499684374153
43	21.198579808428832	22.905204957197142	26.35513272503624	29.541082509337784
44	20.70043988013703	22.553774910115248	30.093614876581558	26.652170333166165
45	21.134331858749324	21.191344117008615	27.559501086601244	30.114822937640817
46	24.23083352670095	24.967876025160248	27.923780723619046	22.87750972451976
47	16.79965368483823	24.632539153823316	33.3620265924135	25.20578056892495
48	19.919109960053994	25.62295560529054	28.14883567744785	26.30909875720762
49	20.35562175797361	20.235359576437595	33.763357959634824	25.645660705953976
50	21.44671412282212	21.604402294462698	28.94950235908491	27.99938122363027
51	19.49993887088283	20.427978672175772	28.394599679134515	31.677482777806887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	393.0
2	684.0
3	1642.0
4	2600.0
5	1824.5
6	1049.0
7	1067.0
8	1085.0
9	1124.0
10	1163.0
11	1161.5
12	1160.0
13	1187.5
14	1215.0
15	1306.5
16	1398.0
17	1383.5
18	1369.0
19	1472.5
20	1576.0
21	1661.5
22	1747.0
23	2379.0
24	3011.0
25	3903.0
26	5727.5
27	6660.0
28	7355.5
29	8051.0
30	10172.5
31	12294.0
32	14156.0
33	16018.0
34	18224.0
35	20430.0
36	22254.0
37	24078.0
38	25896.5
39	27715.0
40	31621.0
41	35527.0
42	39208.0
43	42889.0
44	50500.0
45	58111.0
46	86986.5
47	115862.0
48	102534.5
49	89207.0
50	82845.5
51	76484.0
52	65203.5
53	53923.0
54	49341.5
55	44760.0
56	41392.0
57	38024.0
58	34344.0
59	30664.0
60	26781.5
61	22899.0
62	20525.5
63	18152.0
64	15749.5
65	13347.0
66	10851.5
67	8356.0
68	7106.5
69	5857.0
70	4987.5
71	4118.0
72	3190.5
73	2263.0
74	1808.0
75	1139.5
76	926.0
77	677.5
78	429.0
79	314.5
80	200.0
81	110.5
82	21.0
83	13.0
84	5.0
85	4.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	801582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.487945220433524
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.30599528459656	26.747851402737176
2	9.779278920577422	7.1365158709873855
3	3.7684747727612073	4.1251170321928985
4	2.1781120113385994	3.178993270147644
5	1.6769399138945869	3.0594045858072105
6	1.2916836106369738	2.8278528496232207
7	1.063736613043126	2.7169494285980953
8	0.9199912678150455	2.6854872786650055
9	0.7880937964263326	2.5880330945310766
>10	5.110552941358867	30.353313221245653
>50	0.07087206230207184	1.7498883556045368
>100	0.03378357208671995	2.3714603985099467
>500	0.0055081911010956	1.2999552961378273
>1k	0.0055081911010956	3.8655648622527643
>5k	7.344254801460799E-4	1.8624385298202224
>10k+	7.344254801460799E-4	3.4311745231392994
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	13348	1.6652070530525884	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	12260	1.5294754622733544	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	8291	1.034329613189917	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT	5609	0.6997412616550771	No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2988	0.37276286144149945	Illumina PCR Primer Index 3 (95% over 22bp)
GAATCAGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	2980	0.37176483503871094	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC	2913	0.36340636391535736	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT	2877	0.3589152451028092	No Hit
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2503	0.3122575107724475	RNA PCR Primer, Index 7 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTA	2229	0.27807510647694184	No Hit
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	1940	0.2420214026762078	TruSeq Adapter, Index 7 (95% over 24bp)
GAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT	1666	0.20783899838070213	TruSeq Adapter, Index 7 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	1643	0.20496967247268524	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	1276	0.15918521124476348	No Hit
GCTGTATCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1268	0.158187184841975	TruSeq Adapter, Index 7 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1201	0.14982871371862141	Illumina PCR Primer Index 3 (95% over 22bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCCTCGAATGC	1181	0.14733364771165022	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	1136	0.14171974919596497	No Hit
CCTGTATCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1049	0.1308662120656402	TruSeq Adapter, Index 7 (95% over 23bp)
CTGTCACTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	847	0.10566604539523095	TruSeq Adapter, Index 7 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	808	0.10080066668163706	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2588630982232635	0.0
2	0.0	0.0	0.0	1.1594571734395234	0.0
3	0.0	0.0	0.0	1.524485330259412	0.0
4	0.0	0.0	0.0	2.092487106746409	0.0
5	0.0	0.0	0.0	3.7823953132680126	0.0
6	0.0	0.0	0.0	4.321828583975189	0.0
7	0.0	0.0	0.0	5.333577849802016	0.0
8	0.0	0.0	0.0	6.605188240254896	0.0
9	0.0	0.0	0.0	6.9861848195194005	0.0
10	0.0	0.0	0.0	9.549740388381975	0.0
11	0.0	0.0	0.0	10.954587303607116	0.0
12	0.0	0.0	0.0	14.305710457570155	0.0
13	0.0	0.0	0.0	14.836161490652236	0.0
14	0.0	0.0	0.0	15.089909703561208	0.0
15	0.0	0.0	0.0	15.6668937176733	0.0
16	0.0	0.0	0.0	16.323969350609172	0.0
17	0.0	0.0	0.0	17.09107739445247	0.0
18	0.0	0.0	0.0	17.84683288796405	0.0
19	0.0	0.0	0.0	19.31867232547637	0.0
20	0.0	0.0	0.0	19.990718354454067	0.0
21	0.0	0.0	0.0	20.767432402424205	0.0
22	0.0	0.0	0.0	21.59292499083063	0.0
23	0.0	0.0	0.0	22.35154981025023	0.0
24	0.0	0.0	0.0	22.999643205561004	0.0
25	1.247533003485607E-4	0.0	0.0	23.529844732042385	0.0
26	1.247533003485607E-4	0.0	0.0	24.010020185083995	0.0
27	2.495066006971214E-4	0.0	0.0	24.493189717333973	0.0
28	2.495066006971214E-4	0.0	0.0	24.9762344962836	0.0
29	2.495066006971214E-4	0.0	0.0	25.51791332639705	0.0
30	2.495066006971214E-4	0.0	0.0	26.099637965922387	0.0
31	2.495066006971214E-4	0.0	0.0	26.602643272927786	0.0
32	2.495066006971214E-4	0.0	0.0	27.070093889333844	0.0
33	3.742599010456822E-4	0.0	0.0	27.541536611351052	0.0
34	3.742599010456822E-4	0.0	0.0	28.053399402681197	0.0
35	3.742599010456822E-4	0.0	0.0	28.570127572724935	0.0
36	3.742599010456822E-4	0.0	0.0	29.070637813723362	0.0
37	3.742599010456822E-4	0.0	0.0	29.539211209832555	0.0
38	3.742599010456822E-4	0.0	0.0	30.048828441756427	0.0
39	3.742599010456822E-4	0.0	0.0	30.54434855074091	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAC	35	1.2113196E-7	45.000004	30
ACGACCG	25	3.8897448E-5	45.000004	38
TACGATA	20	7.032009E-4	45.0	25
CTACGAT	20	7.032009E-4	45.0	24
CGTACAG	20	7.032009E-4	45.0	30
CGTAAGC	55	1.8189894E-12	45.0	43
CTTTACG	150	0.0	43.500004	1
GCGAACC	100	0.0	42.750004	33
CTCGAAT	390	0.0	42.692307	43
ATCAGTC	380	0.0	42.63158	3
ACGGGCG	95	0.0	42.63158	5
CGATGAA	2755	0.0	42.63158	19
CCGATGA	2720	0.0	42.6011	18
ATGAATG	2890	0.0	42.430794	21
TCTACGG	175	0.0	42.428574	2
TCGTTAG	85	0.0	42.35294	1
TACGGCT	1865	0.0	42.345844	7
TAATACG	630	0.0	42.142857	4
AATACGG	625	0.0	42.120003	5
GATGAAT	2960	0.0	42.035473	20
>>END_MODULE