##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934163.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1445999
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.015261421342615	33.0	31.0	34.0	30.0	34.0
2	32.20926639644979	33.0	31.0	34.0	30.0	34.0
3	32.28457211934448	34.0	31.0	34.0	30.0	34.0
4	35.75088571983798	37.0	35.0	37.0	35.0	37.0
5	29.64492921502712	37.0	35.0	37.0	0.0	37.0
6	23.848217045793255	35.0	15.0	37.0	0.0	37.0
7	30.461157995268323	35.0	19.0	37.0	17.0	37.0
8	34.1952795264727	35.0	32.0	37.0	32.0	37.0
9	36.92523369656549	38.0	37.0	39.0	34.0	39.0
10	37.05535550162898	39.0	37.0	39.0	34.0	39.0
11	37.20560525975468	39.0	37.0	39.0	34.0	39.0
12	37.160168852122304	39.0	37.0	39.0	34.0	39.0
13	37.08543781842173	39.0	37.0	39.0	33.0	39.0
14	38.11601529461639	40.0	38.0	41.0	33.0	41.0
15	37.99930912815292	40.0	37.0	41.0	33.0	41.0
16	38.302865354678666	40.0	38.0	41.0	33.0	41.0
17	38.2936613372485	40.0	37.0	41.0	34.0	41.0
18	38.36851408610933	40.0	38.0	41.0	34.0	41.0
19	38.352826661705855	40.0	37.0	41.0	34.0	41.0
20	38.18182516032169	40.0	37.0	41.0	34.0	41.0
21	38.123690265345964	40.0	37.0	41.0	34.0	41.0
22	38.218758795822126	40.0	37.0	41.0	34.0	41.0
23	38.102136308531335	40.0	37.0	41.0	34.0	41.0
24	38.097231740824164	40.0	37.0	41.0	34.0	41.0
25	37.88175994589208	40.0	36.0	41.0	33.0	41.0
26	37.75844519947801	40.0	36.0	41.0	33.0	41.0
27	36.90003174276054	40.0	35.0	41.0	32.0	41.0
28	37.24204719367026	40.0	35.0	41.0	32.0	41.0
29	37.500892462581234	40.0	35.0	41.0	32.0	41.0
30	37.49488346810752	40.0	36.0	41.0	33.0	41.0
31	37.50631916066332	40.0	36.0	41.0	33.0	41.0
32	37.40476791477726	40.0	36.0	41.0	33.0	41.0
33	36.16851048997959	39.0	35.0	41.0	30.0	41.0
34	36.50573271489123	39.0	35.0	41.0	30.0	41.0
35	36.77042446087445	39.0	35.0	41.0	30.0	41.0
36	36.88521153887382	39.0	35.0	41.0	31.0	41.0
37	36.99024273184145	39.0	35.0	41.0	32.0	41.0
38	36.88050752455569	39.0	35.0	41.0	31.0	41.0
39	36.849431431142065	39.0	35.0	41.0	32.0	41.0
40	36.82067276671699	39.0	35.0	41.0	31.0	41.0
41	36.69416922141716	39.0	35.0	41.0	31.0	41.0
42	36.7230703479048	39.0	35.0	41.0	31.0	41.0
43	36.60236417867509	39.0	35.0	41.0	31.0	41.0
44	36.672004614111074	39.0	35.0	41.0	31.0	41.0
45	36.58388007183961	39.0	35.0	41.0	31.0	41.0
46	36.53919885145149	39.0	35.0	41.0	31.0	41.0
47	36.45369187668871	39.0	35.0	41.0	31.0	41.0
48	36.44083225507072	39.0	35.0	41.0	31.0	41.0
49	36.44154871476398	39.0	35.0	40.0	31.0	41.0
50	36.335797604286036	39.0	35.0	40.0	31.0	41.0
51	35.67544998302212	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	15.0
10	12.0
11	17.0
12	21.0
13	21.0
14	31.0
15	38.0
16	86.0
17	135.0
18	269.0
19	562.0
20	1348.0
21	2543.0
22	4311.0
23	6407.0
24	8414.0
25	10648.0
26	12516.0
27	13505.0
28	14656.0
29	17698.0
30	22863.0
31	31601.0
32	45611.0
33	71628.0
34	98001.0
35	119105.0
36	158350.0
37	239811.0
38	291877.0
39	273675.0
40	222.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.0495339208395	4.015977881035878	52.49436548711306	17.440122711011558
2	20.204370819066956	8.330780311742954	51.11033963370652	20.354509235483565
3	19.68452260340429	8.301527179479377	51.295747784057944	20.71820243305839
4	17.670275014021446	4.39011368610905	53.98371644793668	23.955894851932815
5	17.155682680278478	22.021799461825353	40.74975155584478	20.072766302051384
6	15.994409401389628	31.50064419131687	39.24594691974199	13.258999487551511
7	81.19971037324369	1.7676360772033728	12.777602197511891	4.255051352041046
8	82.76077645973476	4.965632756315876	8.26155481435326	4.012035969596106
9	78.1216999458506	4.160168852122304	9.52905223309283	8.189078968934279
10	41.08170199287828	26.177403995438446	17.41425823945936	15.32663577222391
11	31.44199961410762	23.952437034880383	25.3911655540564	19.2143977969556
12	25.79503858578049	20.82601716875323	30.41260747759853	22.96633676786775
13	26.17512183618384	20.423873045555357	33.3520977538712	20.0489073643896
14	20.95651518431202	24.146904665909176	33.246150239384676	21.650429910394127
15	18.87954279359806	22.13901945990281	36.52070298803803	22.460734758461108
16	23.13079054688143	22.53058266292024	30.5931055277355	23.745521262462837
17	23.103404635826166	23.743584884913474	30.1998825725329	22.95312790672746
18	23.1169592786717	22.59026458524522	30.651888417626843	23.640887718456234
19	23.79289335608116	23.711219717302708	28.626368344653073	23.86951858196306
20	24.894484712645028	26.30153962762077	29.428858526181557	19.375117133552653
21	23.78777578684356	26.263365327361914	30.289854972237183	19.659003913557342
22	22.94434505141428	21.294966317404093	32.48626036394216	23.274428267239465
23	22.78922737844217	24.272907519299807	30.751266079713744	22.186599022544275
24	21.97449652454808	25.621663638771537	29.18660386348815	23.217235973192235
25	22.625741788203175	27.492550133160538	26.7590779799986	23.12263009863769
26	21.07767709382925	24.68819134729692	28.9567973421835	25.277334216690328
27	21.910803534442277	26.11461003776628	28.548498304632297	23.426088123159143
28	19.37670772939677	25.905273793412032	29.67692232152304	25.041096155668157
29	21.777124327195246	25.11502428424916	29.000365837044146	24.107485551511445
30	22.43203487692592	25.677403649656743	29.187917833968076	22.702643639449267
31	22.880375435944288	25.697044050514556	27.598635960329155	23.823944553212
32	25.843448024514537	25.76626954790425	26.06696131878376	22.323321108797447
33	24.354650314419306	26.050847891319428	26.302023722008105	23.29247807225316
34	21.360526528718207	25.192133604518396	29.741860125767722	23.70547974099567
35	22.41903348480877	25.26184319629543	27.523324704927184	24.79579861396861
36	23.26198012585071	27.789991556010758	26.094139760815878	22.853888557322655
37	22.10402635133219	26.16869029646632	27.877958421824633	23.849324930376852
38	23.255963524179478	25.15893856081505	27.37892626481761	24.206171650187862
39	24.073460631715513	23.624151883922465	28.525676712086245	23.776710772275774
40	22.51121888742662	22.090402552145612	31.136743524718895	24.261635035708874
41	21.168271900602974	24.000431535568143	28.31468071554683	26.516615848282054
42	21.71260146099686	23.438328795524754	29.345041040830594	25.50402870264779
43	23.10063838218422	23.65762355299001	28.917793165832066	24.32394489899371
44	22.861219129473813	23.004096130080313	28.987226132244903	25.147458608200974
45	21.902089835470147	22.898148615593787	27.340475339194565	27.8592862097415
46	22.88576963054608	24.061634897396196	28.50250933783495	24.550086134222777
47	19.458173899152072	25.070418444272786	31.826093932291794	23.64531372428335
48	20.615297797578005	25.552645610404984	29.21412808722551	24.617928504791497
49	22.25582451993397	23.085078205448276	30.94995224754651	23.709145027071248
50	21.706377390302485	23.08231195180633	29.440545947818777	25.770764710072413
51	20.399391700824136	22.869102952353355	28.90783465272106	27.823670694101448
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	297.0
1	910.0
2	1523.0
3	6467.5
4	11412.0
5	7740.5
6	4069.0
7	3930.5
8	3792.0
9	3864.5
10	3937.0
11	3921.0
12	3905.0
13	3911.0
14	3917.0
15	3756.0
16	3595.0
17	3695.5
18	3796.0
19	3867.0
20	3938.0
21	4165.5
22	4393.0
23	5391.5
24	6390.0
25	7776.5
26	10942.5
27	12722.0
28	15326.5
29	17931.0
30	20712.0
31	23493.0
32	28012.5
33	32532.0
34	36558.5
35	40585.0
36	42877.0
37	45169.0
38	48573.0
39	51977.0
40	56418.0
41	60859.0
42	66568.5
43	72278.0
44	81467.5
45	90657.0
46	116703.5
47	142750.0
48	140666.5
49	138583.0
50	133566.0
51	128549.0
52	116202.0
53	103855.0
54	95789.5
55	87724.0
56	82082.0
57	76440.0
58	71015.5
59	65591.0
60	59008.5
61	52426.0
62	47058.0
63	41690.0
64	36722.5
65	31755.0
66	27177.5
67	22600.0
68	19041.5
69	15483.0
70	13031.0
71	10579.0
72	8889.0
73	7199.0
74	5779.5
75	3304.0
76	2248.0
77	1640.5
78	1033.0
79	746.0
80	459.0
81	329.5
82	200.0
83	146.0
84	92.0
85	66.5
86	41.0
87	26.5
88	12.0
89	6.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1445999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.443748997627864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.87524768257788	26.194034856862025
2	9.197293703320648	6.70367726362562
3	3.700965615230573	4.046311857909432
4	2.3765875328198707	3.464470380679162
5	1.8147531205848826	3.3068203609628677
6	1.506358127070268	3.2938402490091403
7	1.266971450863523	3.2321232679701453
8	1.079385944562398	3.14694963481596
9	0.9105814796895021	2.9866502598923517
>10	6.172836211355288	36.00324743345042
>50	0.06503384605303376	1.6210175791200099
>100	0.028037862450508048	1.950330977370183
>500	0.002761303731498347	0.6801966688244636
>1k	0.0025488957521523203	1.7871515298907923
>5k	6.372239380380801E-4	1.5831776796173813
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGC	7716	0.5336103275313469	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCG	6382	0.44135576857245407	No Hit
GAATCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTC	6354	0.43941939102309197	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3685	0.2548411167642578	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAGTTCGT	2729	0.1887276547217529	No Hit
GAATCAGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTC	2228	0.15408032785638165	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	1936	0.13388667627017722	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTC	1869	0.129253201419918	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1717	0.11874143758052391	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGC	1706	0.11798071782898882	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCT	1700	0.11756577978269694	No Hit
CTGTCTCTTATACACATCTGACGCACAGTTCGTCGTATGCCGTCTTCTGCT	1643	0.11362386834292418	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12206094195085888	0.0
2	0.0	0.0	0.0	0.4634166413669719	0.0
3	0.0	0.0	0.0	0.6710931335360536	0.0
4	0.0	0.0	0.0	1.0105124554028047	0.0
5	0.0	0.0	0.0	1.944053903218467	0.0
6	0.0	0.0	0.0	2.5381760291673783	0.0
7	0.0	0.0	0.0	3.2885223295451795	0.0
8	0.0	0.0	0.0	4.307679327579065	0.0
9	0.0	0.0	0.0	4.797582847567668	0.0
10	0.0	0.0	0.0	6.093918460524523	0.0
11	0.0	0.0	0.0	7.392812858100179	0.0
12	0.0	0.0	0.0	9.13133411572207	0.0
13	0.0	0.0	0.0	9.561486557044645	0.0
14	0.0	0.0	0.0	9.774764712838667	0.0
15	0.0	0.0	0.0	10.11100284301718	0.0
16	0.0	0.0	0.0	10.570131791239136	0.0
17	0.0	0.0	0.0	11.13299525103406	0.0
18	0.0	0.0	0.0	11.717850427282453	0.0
19	0.0	0.0	0.0	12.407269991196397	0.0
20	0.0	0.0	0.0	12.92891627172633	0.0
21	0.0	0.0	0.0	13.58991257946928	0.0
22	6.915634104864526E-5	0.0	0.0	14.313426219520208	0.0
23	1.3831268209729051E-4	0.0	0.0	14.963495825377473	0.0
24	1.3831268209729051E-4	0.0	0.0	15.495377244382603	0.0
25	1.3831268209729051E-4	0.0	0.0	15.952362346032052	0.0
26	1.3831268209729051E-4	0.0	0.0	16.384589477586083	0.0
27	1.3831268209729051E-4	0.0	0.0	16.859347758885033	0.0
28	1.3831268209729051E-4	0.0	0.0	17.298974618931272	0.0
29	1.3831268209729051E-4	0.0	0.0	17.798214244961443	0.0
30	1.3831268209729051E-4	0.0	0.0	18.37318006443988	0.0
31	1.3831268209729051E-4	0.0	0.0	18.838394770674114	0.0
32	1.3831268209729051E-4	0.0	0.0	19.291230491860645	0.0
33	2.0746902314593577E-4	0.0	0.0	19.743927900365076	0.0
34	2.7662536419458103E-4	0.0	0.0	20.247731844904457	0.0
35	2.7662536419458103E-4	0.0	0.0	20.797732225264333	0.0
36	2.7662536419458103E-4	0.0	0.0	21.2691018458519	0.0
37	2.7662536419458103E-4	0.0	0.0	21.76986291138514	0.0
38	2.7662536419458103E-4	6.915634104864526E-5	0.0	22.350845332534806	0.0
39	2.7662536419458103E-4	6.915634104864526E-5	0.0	23.335700785408566	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTATC	20	7.0338923E-4	45.0	38
CGTTAGG	370	0.0	43.175674	2
CGTTTAT	920	0.0	42.554344	1
AATACGG	365	0.0	41.91781	5
CGTTATT	795	0.0	41.88679	1
TTAGGGA	3130	0.0	41.765175	4
CGAACGA	65	0.0	41.53846	22
CCGATGA	1590	0.0	41.462265	18
CGATGAA	1615	0.0	41.099075	19
TTATGGG	4115	0.0	40.407047	3
CGTTTTT	2235	0.0	40.369125	1
TCGTTAG	230	0.0	40.108692	1
TAGGGAC	1270	0.0	40.03937	5
TATGGGA	2505	0.0	39.97006	4
TAGGGAG	1735	0.0	39.293945	5
CTACGGG	570	0.0	39.07895	3
TAATACG	400	0.0	38.812504	4
TTTATGG	2610	0.0	38.706894	2
TATGGGC	1065	0.0	38.661972	4
TTTACGG	810	0.0	38.61111	2
>>END_MODULE