##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934161.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	571237
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.948882862979815	33.0	31.0	34.0	30.0	34.0
2	32.17372649180638	33.0	31.0	34.0	30.0	34.0
3	32.24889844320308	34.0	31.0	34.0	30.0	34.0
4	35.68418712373323	37.0	35.0	37.0	33.0	37.0
5	29.571920236259206	37.0	35.0	37.0	0.0	37.0
6	23.64071129846281	35.0	10.0	37.0	0.0	37.0
7	30.34781360451091	35.0	19.0	37.0	17.0	37.0
8	34.22214772502481	35.0	32.0	37.0	32.0	37.0
9	36.85194761543808	38.0	37.0	39.0	34.0	39.0
10	36.98163984475796	39.0	37.0	39.0	34.0	39.0
11	37.102873238253125	39.0	37.0	39.0	34.0	39.0
12	37.1000968074547	39.0	37.0	39.0	34.0	39.0
13	36.998035841515865	39.0	37.0	39.0	33.0	39.0
14	38.09223317117063	40.0	37.0	41.0	33.0	41.0
15	37.98636817993232	40.0	37.0	41.0	33.0	41.0
16	38.2131672143086	40.0	37.0	41.0	34.0	41.0
17	38.22734171631039	40.0	37.0	41.0	34.0	41.0
18	38.30029217295098	40.0	37.0	41.0	34.0	41.0
19	38.3146312301199	40.0	37.0	41.0	34.0	41.0
20	38.28823062931848	40.0	37.0	41.0	34.0	41.0
21	38.13619040783423	40.0	37.0	41.0	34.0	41.0
22	38.204753893742875	40.0	37.0	41.0	34.0	41.0
23	38.15858041408382	40.0	37.0	41.0	34.0	41.0
24	38.12901475219567	40.0	37.0	41.0	34.0	41.0
25	37.83840682588838	40.0	36.0	41.0	33.0	41.0
26	37.65790206166617	40.0	36.0	41.0	33.0	41.0
27	36.74731678795316	39.0	35.0	41.0	32.0	41.0
28	37.03344846359742	39.0	35.0	41.0	31.0	41.0
29	37.33559975981948	39.0	35.0	41.0	32.0	41.0
30	37.35922568040936	39.0	35.0	41.0	33.0	41.0
31	37.36701229087051	39.0	35.0	41.0	33.0	41.0
32	37.41002946237726	39.0	35.0	41.0	33.0	41.0
33	36.274905161955544	39.0	35.0	41.0	31.0	41.0
34	36.69890255708226	39.0	35.0	41.0	31.0	41.0
35	37.00602201888183	39.0	35.0	41.0	31.0	41.0
36	37.07088476411717	39.0	35.0	41.0	32.0	41.0
37	37.139141897321075	39.0	35.0	41.0	32.0	41.0
38	37.119115183365224	39.0	35.0	41.0	32.0	41.0
39	37.23565000166306	39.0	35.0	41.0	33.0	41.0
40	37.212743572282605	39.0	35.0	40.0	33.0	41.0
41	37.14881739103034	39.0	35.0	40.0	33.0	41.0
42	37.16680292067916	39.0	35.0	40.0	33.0	41.0
43	36.99988796243941	39.0	35.0	40.0	32.0	41.0
44	36.99833344128619	39.0	35.0	40.0	32.0	41.0
45	36.93904456469031	39.0	35.0	40.0	32.0	41.0
46	36.851263135966335	39.0	35.0	40.0	32.0	41.0
47	36.79499927350644	39.0	35.0	40.0	32.0	41.0
48	36.76916936402929	39.0	35.0	40.0	32.0	41.0
49	36.81777440887058	39.0	35.0	40.0	32.0	41.0
50	36.75464299406376	39.0	35.0	40.0	32.0	41.0
51	36.116112926858726	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	9.0
12	8.0
13	8.0
14	15.0
15	17.0
16	16.0
17	36.0
18	66.0
19	126.0
20	220.0
21	372.0
22	611.0
23	944.0
24	1341.0
25	2118.0
26	2885.0
27	3707.0
28	4824.0
29	6534.0
30	9283.0
31	12976.0
32	19068.0
33	31710.0
34	43113.0
35	57030.0
36	71156.0
37	103585.0
38	113512.0
39	85882.0
40	58.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.115449454429598	5.105761706612142	53.56533277781376	18.213456061144498
2	19.558256905627612	4.487454419094001	52.68583792716508	23.268450748113306
3	20.03966829879717	4.5025094662985765	52.38648756995783	23.071334664946423
4	19.413483370299893	4.611746087875961	51.04431260580109	24.93045793602305
5	15.294527490341137	22.0468912202816	40.93362299710978	21.72495829226748
6	16.672239368248203	29.64128724154773	38.65033252397866	15.036140866225404
7	81.01225935995042	2.404956261586697	10.573019604822518	6.009764773640363
8	81.6281158258306	2.9656692406129155	8.687287413105244	6.718927520451231
9	75.88759131498834	5.035213055176748	12.314503437277349	6.762692192557555
10	41.97504713455186	23.71520052097466	18.620642570421733	15.689109774051751
11	29.664570047108292	24.47407993529831	24.853782230492772	21.007567787100626
12	27.00805445025445	20.709092723335498	30.635795650491826	21.647057175918228
13	23.512482559778167	23.71327487540198	31.61927536206513	21.154967202754722
14	19.65681494721105	25.708943923450338	31.483954995912377	23.15028613342623
15	17.85055940003886	23.816209384196053	35.519057764115416	22.814173451649665
16	19.333831667066384	22.75150244119341	33.26430185719762	24.65036403454258
17	18.590532476012584	23.168492237022463	30.121123106521463	28.119852180443495
18	19.826621874983587	23.50688068174856	31.823918968834302	24.842578474433555
19	22.932513125025164	24.94026122257487	29.181233008366053	22.945992644033915
20	25.892055311543196	24.24737893378755	28.565726659862715	21.294839094806534
21	23.72920521604868	25.6734070097	29.23287532145152	21.3645124527998
22	22.016255949807174	23.681064076731726	27.64404266530354	26.658637308157562
23	22.29057291456961	25.085209816591014	26.508261894800235	26.115955374039146
24	23.431430387037256	22.331536647661128	28.238366912507416	25.9986660527942
25	21.164245313241263	25.102015450679843	26.634129091778018	27.09961014430088
26	19.883515948721808	28.122478060769872	27.192041131789434	24.801964858718886
27	19.539525625966107	30.559995238403676	26.63185332882849	23.26862580680173
28	18.675085822522	27.415766135596957	30.295306501504633	23.613841540376413
29	19.443243347332192	26.21696423726054	29.368020628915843	24.97177178649142
30	20.739377876433075	26.94083191389913	27.406312966421993	24.9134772432458
31	20.90253257404545	28.597937458532975	23.638874932821228	26.86065503460035
32	21.660536694926975	27.65262054103638	24.038533918496178	26.64830884554047
33	20.98516027498219	28.326946608850616	23.023018466941043	27.66487464922615
34	18.84786874799777	26.286287477876957	26.557453386247737	28.308390387877537
35	18.481120795746776	25.955951732818427	25.55856851009301	30.004358961341786
36	20.14540374660605	29.35821734236403	23.67336149444101	26.82301741658891
37	19.413658428988317	29.626582311720007	26.463446870563356	24.496312388728324
38	19.243501383838932	27.478787263430064	26.619074044573445	26.658637308157562
39	20.22960697573862	23.48744916733335	28.696495500116413	27.586448356811623
40	20.689836267608715	22.336263232248612	28.077663036532996	28.896237463609676
41	18.027893851413687	24.5279980113333	25.55699298189718	31.887115155355833
42	19.944261313605384	24.03100639489389	25.240311814535822	30.784420476964904
43	21.081967729681374	24.223921069538562	27.475461148349982	27.21865005243008
44	19.807365419256804	24.41053363139993	27.42539436346035	28.356706585882918
45	20.24203614261681	23.701721001965907	26.49863366693684	29.557609188480438
46	20.266194241619502	25.23768593420944	27.778662796702598	26.717457027468463
47	17.321707102306046	25.447581301631374	29.27926587388422	27.95144572217836
48	17.79489073711962	23.43073015228355	29.92995901876104	28.844420091835786
49	19.29566887298967	22.461430194472698	30.722974877327623	27.519926055210007
50	19.625479441982925	22.079802253705555	28.389967736683726	29.904750567627797
51	17.858437041018004	21.853976545636925	26.3412559060425	33.94633050730258
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	105.0
1	406.5
2	708.0
3	1165.0
4	1622.0
5	1196.5
6	771.0
7	829.0
8	887.0
9	1015.0
10	1143.0
11	1152.0
12	1161.0
13	1158.0
14	1155.0
15	1216.0
16	1277.0
17	1230.5
18	1184.0
19	1228.0
20	1272.0
21	1263.5
22	1255.0
23	1504.0
24	1753.0
25	2335.5
26	3593.5
27	4269.0
28	4856.0
29	5443.0
30	6585.5
31	7728.0
32	8672.5
33	9617.0
34	10952.0
35	12287.0
36	13096.5
37	13906.0
38	15624.0
39	17342.0
40	21284.5
41	25227.0
42	29930.0
43	34633.0
44	38921.0
45	43209.0
46	49788.5
47	56368.0
48	63840.5
49	71313.0
50	66374.5
51	61436.0
52	50783.0
53	40130.0
54	34475.5
55	28821.0
56	27296.5
57	25772.0
58	24913.0
59	24054.0
60	22733.0
61	21412.0
62	18755.0
63	16098.0
64	13799.0
65	11500.0
66	10018.5
67	8537.0
68	7239.5
69	5942.0
70	4767.0
71	3592.0
72	2887.5
73	2183.0
74	1874.0
75	1208.5
76	852.0
77	626.0
78	400.0
79	282.5
80	165.0
81	159.0
82	153.0
83	100.0
84	47.0
85	26.0
86	5.0
87	9.5
88	14.0
89	10.0
90	6.0
91	3.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	571237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.983688697940906
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.1231952741631	29.968582717150444
2	10.907913629871793	8.940930731013843
3	4.137962308154409	5.0876687724364
4	2.2517757889654164	3.691443118100756
5	1.502491975142931	3.078883169020613
6	1.1884607873971873	2.922450416423763
7	0.9220521707920901	2.6452369391702564
8	0.7728339146904408	2.5338867659907236
9	0.6390906966462458	2.3573064744989933
>10	4.4387407591866195	31.59375539328072
>50	0.0783007666834162	2.241856053152059
>100	0.0328075837997361	2.6181580004471234
>500	0.002624606703978888	0.8042356059987534
>1k	0.001749737802652592	1.5156058433155288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	2645	0.4630302308849042	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	2544	0.44534930335394945	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	1761	0.30827835031694373	TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	1504	0.26328826739164307	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	914	0.16000364122071925	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCACTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	870	0.15230105893000628	TruSeq Adapter, Index 19 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTG	786	0.13759612910228153	No Hit
TCTGTATCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	783	0.13707095303700567	TruSeq Adapter, Index 19 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	605	0.10591050649730323	TruSeq Adapter, Index 19 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4817615105464107	0.0
2	0.0	0.0	0.0	1.6332975630079984	0.0
3	0.0	0.0	0.0	2.2680603672381165	0.0
4	0.0	0.0	0.0	2.8993219976997286	0.0
5	0.0	0.0	0.0	3.9528251846431517	0.0
6	0.0	0.0	0.0	4.747591630093989	0.0
7	0.0	0.0	0.0	5.759430849192191	0.0
8	0.0	0.0	0.0	7.362443259102614	0.0
9	0.0	0.0	0.0	8.094538694097196	0.0
10	0.0	0.0	0.0	9.404677918272101	0.0
11	0.0	0.0	0.0	11.916419979798228	0.0
12	0.0	0.0	0.0	14.133013092639308	0.0
13	1.7505868842529457E-4	0.0	0.0	14.863708058126488	0.0
14	1.7505868842529457E-4	0.0	0.0	15.120694212734819	0.0
15	1.7505868842529457E-4	0.0	0.0	15.491818632196443	0.0
16	1.7505868842529457E-4	0.0	0.0	16.337001979913765	0.0
17	1.7505868842529457E-4	0.0	0.0	17.51952342022663	0.0
18	1.7505868842529457E-4	0.0	0.0	18.94099997024002	0.0
19	1.7505868842529457E-4	0.0	0.0	19.87528819036582	0.0
20	1.7505868842529457E-4	0.0	0.0	20.759859742978833	0.0
21	1.7505868842529457E-4	0.0	0.0	22.04794157241215	0.0
22	1.7505868842529457E-4	0.0	0.0	23.22258537174588	0.0
23	1.7505868842529457E-4	0.0	0.0	24.355390144545957	0.0
24	1.7505868842529457E-4	0.0	0.0	25.330992215140125	0.0
25	1.7505868842529457E-4	0.0	0.0	26.117881019611826	0.0
26	1.7505868842529457E-4	0.0	0.0	26.79185697004921	0.0
27	1.7505868842529457E-4	0.0	0.0	27.4534037536084	0.0
28	1.7505868842529457E-4	0.0	0.0	28.14751145321469	0.0
29	1.7505868842529457E-4	0.0	0.0	28.88888499869581	0.0
30	1.7505868842529457E-4	0.0	0.0	29.660543697274512	0.0
31	3.5011737685058914E-4	0.0	0.0	30.404017947016737	0.0
32	3.5011737685058914E-4	0.0	0.0	31.11825739579194	0.0
33	3.5011737685058914E-4	0.0	0.0	31.809739215071854	0.0
34	3.5011737685058914E-4	0.0	0.0	32.46866011830466	0.0
35	3.5011737685058914E-4	0.0	0.0	33.163468052664655	0.0
36	3.5011737685058914E-4	0.0	0.0	33.84217058768952	0.0
37	3.5011737685058914E-4	0.0	0.0	34.56778885121237	0.0
38	3.5011737685058914E-4	0.0	0.0	35.250692794759445	0.0
39	3.5011737685058914E-4	0.0	0.0	35.95810495468606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	40	6.8048394E-9	45.000004	1
CAAACGG	20	7.0303027E-4	45.000004	11
TGGACGT	20	7.0303027E-4	45.000004	39
GAATCGC	20	7.0303027E-4	45.000004	16
AACGGGC	20	7.0303027E-4	45.000004	4
ACGGATG	20	7.0303027E-4	45.000004	38
CGAATGT	20	7.0303027E-4	45.000004	29
TCCGTTC	20	7.0303027E-4	45.000004	14
ACTCGTA	20	7.0303027E-4	45.000004	33
GCGATAT	20	7.0303027E-4	45.000004	9
TTACGAG	20	7.0303027E-4	45.000004	1
TTACGAA	20	7.0303027E-4	45.000004	26
TGAATCG	20	7.0303027E-4	45.000004	15
CCACGCG	20	7.0303027E-4	45.000004	14
AATCGCG	20	7.0303027E-4	45.000004	17
ATCTCGC	25	3.888333E-5	45.0	31
CGTTATT	85	0.0	45.0	1
GTAGCGT	25	3.888333E-5	45.0	16
CCGTACG	25	3.888333E-5	45.0	1
CGTAGCA	25	3.888333E-5	45.0	20
>>END_MODULE