FastQCFastQC Report
Sat 14 Jan 2017
SRR2934160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931784
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC184740.9563180976755166No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG153180.7929457951820701No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC144280.7468743917539435No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGGGTT68590.3550604001275505No Hit
GAATCAGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC55220.28584976374170196No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.26586823371557067No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTC48560.25137385960335107No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT43080.2230062988408642No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC41850.21663912735585344No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT38220.19784820663179734No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT34560.17890198904225316Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC32400.16772061472711236No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTA31200.16150874010758967No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22480.11636911787239153No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC20850.10793132151420655No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG253.8917948E-545.016
CGATCGA3700.045.041
CGTTATT8750.043.457141
CCGATCG4100.042.8048840
CGTTTAT11400.042.236841
CCGATGA31150.041.532918
TACGGCT28600.041.0664337
AATACGG10050.040.970155
TAGGGCG8150.040.582825
CCGCGTA501.0822987E-940.540
TTATGGG45850.040.4852753
TATGGGC13400.040.4664154
GATGAAT35650.040.20336520
TCGTTAG2800.040.1785741
TAGGGAT13400.040.1305965
TAATACG10400.040.024044
CGATGAA33100.039.96978819
GTTTACG2550.039.7058831
TTACGGG16550.039.5619353
TATGGGA22050.039.1836744
Produced by FastQC (version 0.11.5)