##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934160.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1931784
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85422593830366	33.0	31.0	34.0	30.0	34.0
2	31.986162013972578	33.0	31.0	34.0	30.0	34.0
3	32.07509172868188	33.0	31.0	34.0	30.0	34.0
4	35.63916255647629	37.0	35.0	37.0	33.0	37.0
5	29.58917974266274	37.0	35.0	37.0	0.0	37.0
6	23.825062222277438	35.0	17.0	37.0	0.0	37.0
7	30.397202792858828	35.0	19.0	37.0	17.0	37.0
8	33.9536350854961	35.0	32.0	37.0	32.0	37.0
9	36.739048465045784	37.0	35.0	39.0	33.0	39.0
10	36.938990591080575	39.0	37.0	39.0	34.0	39.0
11	37.065460217084315	39.0	37.0	39.0	34.0	39.0
12	36.94947054121993	39.0	35.0	39.0	33.0	39.0
13	36.81505126867186	39.0	35.0	39.0	33.0	39.0
14	37.68643751061195	40.0	37.0	41.0	32.0	41.0
15	37.5769910093468	40.0	36.0	41.0	32.0	41.0
16	37.94977699370116	40.0	37.0	41.0	33.0	41.0
17	37.93358677781781	40.0	36.0	41.0	33.0	41.0
18	38.012411843146026	40.0	37.0	41.0	33.0	41.0
19	38.000937993067545	40.0	37.0	41.0	33.0	41.0
20	37.75761782890841	40.0	36.0	41.0	33.0	41.0
21	37.756275028678154	40.0	36.0	41.0	33.0	41.0
22	37.880429178417465	40.0	36.0	41.0	33.0	41.0
23	37.7488539091327	40.0	36.0	41.0	33.0	41.0
24	37.747265222198756	40.0	36.0	41.0	33.0	41.0
25	37.57304439833853	39.0	35.0	41.0	33.0	41.0
26	37.440975802677734	39.0	35.0	41.0	33.0	41.0
27	36.53615362794184	39.0	35.0	41.0	31.0	41.0
28	36.89984128660347	39.0	35.0	41.0	31.0	41.0
29	37.11182254330712	39.0	35.0	41.0	31.0	41.0
30	37.10881289005396	39.0	35.0	41.0	32.0	41.0
31	37.13508083719505	39.0	35.0	41.0	32.0	41.0
32	37.010470632327426	39.0	35.0	41.0	32.0	41.0
33	35.801598936527064	39.0	35.0	41.0	30.0	41.0
34	36.05965004369018	39.0	35.0	41.0	29.0	41.0
35	36.274273417732005	39.0	35.0	40.0	30.0	41.0
36	36.26850258621047	39.0	35.0	40.0	30.0	41.0
37	36.32812105287134	39.0	35.0	40.0	30.0	41.0
38	36.37625376336071	39.0	35.0	40.0	30.0	41.0
39	36.32992146119856	39.0	35.0	40.0	31.0	41.0
40	36.31998556774463	39.0	35.0	40.0	30.0	41.0
41	36.1059134975753	38.0	35.0	40.0	30.0	41.0
42	36.154399767261765	38.0	35.0	40.0	30.0	41.0
43	35.94040016896299	38.0	35.0	40.0	30.0	41.0
44	36.13428882318106	38.0	35.0	40.0	31.0	41.0
45	36.123081566055006	38.0	35.0	40.0	31.0	41.0
46	36.05632099655034	38.0	35.0	40.0	31.0	41.0
47	35.95501826291138	38.0	35.0	40.0	30.0	41.0
48	35.94644018171804	38.0	35.0	40.0	31.0	41.0
49	36.00192619878827	38.0	35.0	40.0	31.0	41.0
50	35.790944018585925	38.0	35.0	40.0	30.0	41.0
51	35.155746708741766	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	14.0
10	16.0
11	20.0
12	35.0
13	31.0
14	46.0
15	56.0
16	124.0
17	179.0
18	378.0
19	833.0
20	1944.0
21	4188.0
22	7249.0
23	10323.0
24	13214.0
25	16242.0
26	17938.0
27	19471.0
28	22141.0
29	27216.0
30	36085.0
31	50183.0
32	72648.0
33	113678.0
34	151255.0
35	174397.0
36	227034.0
37	323109.0
38	356030.0
39	285536.0
40	171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.94547216458983	4.019962894402273	48.77817602796172	18.25638891304618
2	19.54659527152104	11.545286636601194	48.757210951120825	20.15090714075694
3	19.245474649339677	11.552274995548157	48.75798743544827	20.444262919663895
4	17.76135427149205	4.424718291486005	54.318702297979485	23.49522513904246
5	19.38674303131199	21.66903753214645	38.42096217796607	20.52325725857549
6	16.473011475403045	32.98904018254628	37.36732471125136	13.170623630799302
7	77.1897893346254	2.0679330608391	15.738146707913515	5.004130896621983
8	77.59847891896815	7.211831136400343	9.856743818149441	5.33294612648205
9	72.92093733046758	4.217138147950288	11.108850678957896	11.753073842624227
10	45.77597702434641	19.578431128946093	18.554765957270586	16.090825889436914
11	34.164948048021934	20.178394685948327	24.917589130047666	20.739068135982077
12	27.29906656230717	17.800282019107726	29.905155027684255	24.995496390900847
13	28.282561611443104	17.64891934087869	34.401827533513064	19.666691514165144
14	21.495053277177988	22.987766748249285	33.61007234763307	21.90710762693966
15	18.711356963304386	19.973661651613224	38.11357791554335	23.201403469539038
16	23.204975297445262	20.721778418291073	29.739815631561296	26.333430652702372
17	24.433373503455876	22.824912101974135	29.75353352134607	22.98818087322392
18	23.910281894870234	20.753355447606978	30.608132172126908	24.728230485395883
19	24.026702778364456	22.80964124353447	28.162258306311678	25.001397671789395
20	24.93896833186319	26.35941699486071	29.62096176384109	19.08065290943501
21	23.914940800834874	26.167625365982943	30.498130225739523	19.419303607442654
22	21.676647078555366	20.540598741888328	33.75035718279062	24.032396996765684
23	23.28629908933918	23.037875870180102	30.669474433994694	23.006350606486027
24	22.960796859276194	24.702037080750227	27.56255357741859	24.774612482554986
25	23.10801828775888	27.31433742074683	25.11600675851959	24.4616375329747
26	20.71789599665387	22.937347032587493	29.200521383343066	27.144235587415572
27	22.555782634083315	25.1943281443474	28.831018374725126	23.41887084684416
28	19.24288636824821	24.911170192940826	29.483886397236958	26.362057041574005
29	22.980001904974884	24.51076310809076	28.480720411805876	24.028514575128483
30	24.538250653282148	26.50384307976461	28.11308096557379	20.844825301379448
31	24.99264928170023	22.995013935305398	27.78131509526945	24.231021687724922
32	30.085713516625045	24.707368939798652	24.264721107535834	20.94219643604047
33	28.68835232096342	24.266843498030834	24.440258331159175	22.604545849846566
34	26.30004182662244	23.038289995154738	27.97673031767527	22.684937860547556
35	26.60970377640564	25.773171327643258	25.567454746493397	22.049670149457704
36	25.8732342746394	27.07207431058545	26.943022615364864	20.11166879941029
37	25.216483830490365	25.76840889043496	27.877340323762905	21.137766955311772
38	27.176744397924406	22.14222708128859	27.314130358259515	23.366898162527487
39	28.599677810769737	21.834066334538438	26.626320541012866	22.939935313678962
40	28.552053438686727	20.1700604208338	27.673332008133418	23.60455413234606
41	25.11403966489007	22.09160030313948	27.274012001341763	25.520348030628682
42	24.710060752133778	21.238916980366334	31.137642717819386	22.913379549680503
43	22.940090610544452	21.883191909654496	30.892428967213725	24.28428851258733
44	23.974419500316806	21.038842851995877	29.516964629585917	25.469773018101407
45	23.606624757219233	19.97718171389762	27.792289407097275	28.62390412178587
46	26.260803485275787	22.322164382767433	27.809009702948156	23.608022429008628
47	20.912431203488588	23.203732922521358	33.22203724640022	22.661798627589835
48	23.062775134280024	24.08333436864577	28.902972589067925	23.95091790800628
49	25.306659543717103	20.296679131828405	31.7724963039346	22.624165020519893
50	24.02390743478567	21.04738417959772	29.011110973069453	25.91759741254716
51	22.891896816621323	21.139268158344827	27.506232580868257	28.4626024441656
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	426.0
1	1295.0
2	2164.0
3	8418.0
4	14672.0
5	9740.5
6	4809.0
7	4633.0
8	4457.0
9	4590.5
10	4724.0
11	4635.5
12	4547.0
13	4445.0
14	4343.0
15	4321.0
16	4299.0
17	4065.0
18	3831.0
19	3844.5
20	3858.0
21	4362.0
22	4866.0
23	5457.5
24	6049.0
25	6844.0
26	9823.0
27	12007.0
28	14150.5
29	16294.0
30	18626.5
31	20959.0
32	25070.5
33	29182.0
34	33190.5
35	37199.0
36	39180.0
37	41161.0
38	46316.5
39	51472.0
40	57684.0
41	63896.0
42	72848.0
43	81800.0
44	99568.5
45	117337.0
46	171260.5
47	225184.0
48	214140.0
49	203096.0
50	195099.0
51	187102.0
52	168106.0
53	149110.0
54	138202.0
55	127294.0
56	118836.5
57	110379.0
58	102200.5
59	94022.0
60	85161.5
61	76301.0
62	69648.0
63	62995.0
64	57031.0
65	51067.0
66	42950.0
67	34833.0
68	30038.5
69	25244.0
70	20933.5
71	16623.0
72	14133.0
73	11643.0
74	9612.0
75	5820.0
76	4059.0
77	2960.0
78	1861.0
79	1378.0
80	895.0
81	612.5
82	330.0
83	215.5
84	101.0
85	75.0
86	49.0
87	32.5
88	16.0
89	12.0
90	8.0
91	4.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1931784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.94467158773402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.49944511408184	27.52357533208169
2	10.104132946879092	7.465877468025023
3	3.5394210971935736	3.922882501395413
4	1.8398342397760132	2.7188828705757238
5	1.2544024997924685	2.317174419683267
6	0.9656500378628271	2.1405374110515036
7	0.8355961115478705	2.1609576744786545
8	0.7029741006975996	2.077691782796438
9	0.6361741488830011	2.115292050277981
>10	5.491515899638397	34.42368179714158
>50	0.0812525051942603	2.0764802026723443
>100	0.04062908522415461	2.767703094417908
>500	0.004062889009262243	1.0245719185704005
>1k	0.003893601967209649	3.1533324113295707
>5k	5.078611261577804E-4	1.0955577034304547
>10k+	5.078611261577804E-4	3.0158013620720743
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC	18474	0.9563180976755166	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCG	15318	0.7929457951820701	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	14428	0.7468743917539435	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGGGTT	6859	0.3550604001275505	No Hit
GAATCAGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTC	5522	0.28584976374170196	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5136	0.26586823371557067	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTC	4856	0.25137385960335107	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCT	4308	0.2230062988408642	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	4185	0.21663912735585344	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCT	3822	0.19784820663179734	No Hit
CTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGCT	3456	0.17890198904225316	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGCCGTCTTCTGC	3240	0.16772061472711236	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGGGTTCGTA	3120	0.16150874010758967	No Hit
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2248	0.11636911787239153	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAATGGGGTTCGTATGC	2085	0.10793132151420655	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.176562182935566E-5	0.0	0.0	0.19117044141581047	0.0
2	5.176562182935566E-5	0.0	0.0	0.7118290657754697	0.0
3	5.176562182935566E-5	0.0	0.0	0.9902763455955739	0.0
4	5.176562182935566E-5	0.0	0.0	1.5175092039275613	0.0
5	5.176562182935566E-5	0.0	0.0	2.9354213514554424	0.0
6	5.176562182935566E-5	0.0	0.0	3.716823412969566	0.0
7	5.176562182935566E-5	0.0	0.0	4.843088047110857	0.0
8	5.176562182935566E-5	0.0	0.0	6.316027050643343	0.0
9	5.176562182935566E-5	0.0	0.0	6.992189602978387	0.0
10	5.176562182935566E-5	0.0	0.0	9.000643964335557	0.0
11	5.176562182935566E-5	0.0	0.0	10.742608904515205	0.0
12	5.176562182935566E-5	0.0	0.0	13.338913667366539	0.0
13	5.176562182935566E-5	0.0	0.0	13.9220016316524	0.0
14	5.176562182935566E-5	0.0	0.0	14.245588533707702	0.0
15	5.176562182935566E-5	0.0	0.0	14.726801754233392	0.0
16	5.176562182935566E-5	0.0	0.0	15.336497248139544	0.0
17	5.176562182935566E-5	0.0	0.0	16.036057861541455	0.0
18	5.176562182935566E-5	0.0	0.0	16.76046597342146	0.0
19	5.176562182935566E-5	0.0	0.0	17.790860675934784	0.0
20	5.176562182935566E-5	0.0	0.0	18.441606307951613	0.0
21	5.176562182935566E-5	0.0	0.0	19.236208603032225	0.0
22	5.176562182935566E-5	0.0	0.0	20.111617033788455	0.0
23	5.176562182935566E-5	0.0	0.0	20.873037565276448	0.0
24	5.176562182935566E-5	0.0	0.0	21.5118253386507	0.0
25	1.5529686548806698E-4	0.0	0.0	22.055933789699054	0.0
26	1.5529686548806698E-4	0.0	0.0	22.563185118004913	0.0
27	2.0706248731742265E-4	0.0	0.0	23.14891312900407	0.0
28	2.0706248731742265E-4	0.0	0.0	23.66574109734836	0.0
29	2.0706248731742265E-4	0.0	0.0	24.270932982155355	0.0
30	2.0706248731742265E-4	0.0	0.0	24.90925486493314	0.0
31	2.0706248731742265E-4	0.0	0.0	25.45486451901455	0.0
32	2.588281091467783E-4	0.0	0.0	25.97474665904677	0.0
33	2.588281091467783E-4	0.0	0.0	26.500374783102046	0.0
34	2.588281091467783E-4	0.0	0.0	27.083618044253395	0.0
35	2.588281091467783E-4	0.0	0.0	27.701181912677608	0.0
36	2.588281091467783E-4	0.0	0.0	28.24233972328169	0.0
37	2.588281091467783E-4	0.0	0.0	28.814039250765095	0.0
38	2.588281091467783E-4	0.0	0.0	29.505369130296142	0.0
39	2.588281091467783E-4	0.0	0.0	30.73951332032981	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	25	3.8917948E-5	45.0	16
CGATCGA	370	0.0	45.0	41
CGTTATT	875	0.0	43.45714	1
CCGATCG	410	0.0	42.80488	40
CGTTTAT	1140	0.0	42.23684	1
CCGATGA	3115	0.0	41.5329	18
TACGGCT	2860	0.0	41.066433	7
AATACGG	1005	0.0	40.97015	5
TAGGGCG	815	0.0	40.58282	5
CCGCGTA	50	1.0822987E-9	40.5	40
TTATGGG	4585	0.0	40.485275	3
TATGGGC	1340	0.0	40.466415	4
GATGAAT	3565	0.0	40.203365	20
TCGTTAG	280	0.0	40.178574	1
TAGGGAT	1340	0.0	40.130596	5
TAATACG	1040	0.0	40.02404	4
CGATGAA	3310	0.0	39.969788	19
GTTTACG	255	0.0	39.705883	1
TTACGGG	1655	0.0	39.561935	3
TATGGGA	2205	0.0	39.183674	4
>>END_MODULE