##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934159.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	943089
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90365066287487	33.0	31.0	34.0	30.0	34.0
2	32.0953971470349	33.0	31.0	34.0	30.0	34.0
3	32.180845074006804	34.0	31.0	34.0	30.0	34.0
4	35.65386299702361	37.0	35.0	37.0	33.0	37.0
5	29.882871075794544	37.0	35.0	37.0	0.0	37.0
6	24.034581041662026	35.0	17.0	37.0	0.0	37.0
7	30.50450275636764	35.0	19.0	37.0	17.0	37.0
8	34.18403565305077	35.0	32.0	37.0	32.0	37.0
9	36.84462336004343	38.0	37.0	39.0	34.0	39.0
10	36.95429169463328	39.0	37.0	39.0	34.0	39.0
11	37.111877033874855	39.0	37.0	39.0	34.0	39.0
12	36.964805018402295	39.0	35.0	39.0	33.0	39.0
13	36.81460286356855	39.0	35.0	39.0	33.0	39.0
14	37.583148568162706	40.0	37.0	41.0	32.0	41.0
15	37.54547768026136	40.0	36.0	41.0	32.0	41.0
16	37.906382112398724	40.0	36.0	41.0	33.0	41.0
17	37.9327422968564	40.0	36.0	41.0	33.0	41.0
18	38.05467140428952	40.0	37.0	41.0	33.0	41.0
19	38.052402265321724	40.0	37.0	41.0	34.0	41.0
20	37.94249641338198	40.0	36.0	41.0	33.0	41.0
21	37.850559173100315	40.0	36.0	41.0	33.0	41.0
22	37.99883362015674	40.0	36.0	41.0	34.0	41.0
23	37.94633910479287	40.0	36.0	41.0	34.0	41.0
24	37.89952485926567	40.0	36.0	41.0	34.0	41.0
25	37.67167785861144	40.0	35.0	41.0	33.0	41.0
26	37.59380609889416	39.0	35.0	41.0	33.0	41.0
27	36.79263144835747	39.0	35.0	41.0	32.0	41.0
28	37.124054039438484	39.0	35.0	41.0	32.0	41.0
29	37.33753124042376	39.0	35.0	41.0	32.0	41.0
30	37.37984325975597	39.0	35.0	41.0	33.0	41.0
31	37.37978494076381	39.0	35.0	41.0	33.0	41.0
32	37.305556527538755	39.0	35.0	41.0	33.0	41.0
33	36.23864873834813	39.0	35.0	41.0	31.0	41.0
34	36.61672970419547	39.0	35.0	41.0	31.0	41.0
35	36.84003100449692	39.0	35.0	41.0	31.0	41.0
36	36.958116360173854	39.0	35.0	41.0	32.0	41.0
37	37.021052095825524	39.0	35.0	41.0	32.0	41.0
38	36.955572591770235	39.0	35.0	41.0	32.0	41.0
39	36.96556316530041	39.0	35.0	41.0	32.0	41.0
40	36.900220445790374	39.0	35.0	41.0	32.0	41.0
41	36.76505292713625	39.0	35.0	41.0	31.0	41.0
42	36.798405028581605	39.0	35.0	41.0	32.0	41.0
43	36.71943157008511	39.0	35.0	40.0	32.0	41.0
44	36.782393814369584	39.0	35.0	40.0	32.0	41.0
45	36.71675843955342	39.0	35.0	40.0	32.0	41.0
46	36.669405538607705	39.0	35.0	40.0	32.0	41.0
47	36.56111459257822	38.0	35.0	40.0	32.0	41.0
48	36.50786617169747	38.0	35.0	40.0	31.0	41.0
49	36.54543632679418	38.0	35.0	40.0	32.0	41.0
50	36.462649866555545	38.0	35.0	40.0	32.0	41.0
51	35.714349335004435	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	13.0
10	10.0
11	6.0
12	16.0
13	21.0
14	17.0
15	18.0
16	51.0
17	99.0
18	149.0
19	297.0
20	573.0
21	1043.0
22	1756.0
23	2649.0
24	3663.0
25	4856.0
26	6087.0
27	7472.0
28	9296.0
29	12438.0
30	16904.0
31	23789.0
32	34555.0
33	55587.0
34	74230.0
35	84011.0
36	107900.0
37	159657.0
38	181776.0
39	154056.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.113866241680267	4.466492557966427	51.522284747250794	17.897356453102518
2	19.498795977898162	7.397923207671811	50.03037889319035	23.072901921239673
3	20.988687175865692	7.405875797512218	50.409134238656165	21.19630278796593
4	19.10466562540757	4.557258116678278	51.303111371249166	25.03496488666499
5	16.91091720929838	21.297141627142295	40.10639504861153	21.685546114947794
6	16.62674466566782	30.77238733565973	38.64439093235103	13.956477066321419
7	80.46069883118136	2.1921578981411085	12.120383123968152	5.226760146709378
8	81.42402254718272	4.337024395364594	8.289567580578291	5.949385476874399
9	76.20351843781445	4.677183171471622	10.812129077955527	8.307169312758393
10	41.84249842803808	24.63203366808435	17.85229177734021	15.673176126537369
11	33.722374028326065	22.299592085158455	24.51412326938391	19.463910617131575
12	28.265731017963308	19.944141008960976	29.755198077806018	22.034929895269695
13	27.27653487634783	21.146784661892994	32.33703287812709	19.239647583632085
14	20.682353415213196	24.071747205194843	33.86467236920375	21.381227010388205
15	19.03383455856234	21.399570984286743	37.079533320821255	22.487061136329658
16	24.389638729748732	21.751181489763958	29.497216063383203	24.361963717104114
17	24.22454296466187	22.723200037324155	28.947214950020623	24.10504204799335
18	25.433654723997417	20.483008496546987	30.205208628241877	23.878128151213723
19	25.21193651924686	23.483785729660724	27.589230708872652	23.71504704221977
20	24.552931907805096	27.511613432030273	28.84171059147122	19.09374406869341
21	24.246492112621397	25.595357384085705	30.73230628286408	19.425844220428825
22	22.19822307332606	21.606550389199747	32.05890430277524	24.13632223469895
23	21.36086838039676	23.80135915062099	30.783839065029916	24.053933403952332
24	22.01531350699669	26.300487016601824	28.049314539773025	23.63488493662846
25	24.10896532564795	26.279174075829538	24.908359656405704	24.70350094211681
26	22.212219631445176	23.531607303234374	26.74721049657031	27.50896256875014
27	21.7071771593137	23.962849741646867	28.903528723163983	25.426444375875445
28	19.144534609140813	24.16283086750031	28.442278512420355	28.25035601093852
29	22.174153234742427	24.724495779295484	27.371541816307897	25.72980916965419
30	24.042693743644556	23.26005286881726	28.83121317288188	23.866040214656305
31	23.242026998512337	23.88215746339953	26.616681988656428	26.259133549431706
32	25.471403017106553	26.475550027621995	24.275121436046863	23.777925519224592
33	23.00832689173556	26.06286363217045	24.776982872242172	26.15182660385181
34	21.11317171550087	24.554946563898	28.298071549980968	26.033810170620164
35	21.251864882317577	27.56579707747625	25.874758373812018	25.307579666394158
36	24.703925080241632	26.432924146077415	24.308310244314164	24.554840529366796
37	20.15048420668675	27.509704810468577	25.96054030955721	26.379270673287465
38	21.10299240050515	27.357333189126372	25.79183937040937	25.74783503995911
39	21.596477108735233	25.030193332760746	26.8758303829225	26.497499175581517
40	23.159638167765713	22.969306184251963	28.978813240319845	24.89224240766248
41	20.316852386148074	24.868278603610054	25.09042094648543	29.72444806375644
42	20.80598967859873	25.09423818960883	27.92355758576338	26.17621454602906
43	21.688515081821546	23.777077242974947	28.275910332959032	26.258497342244475
44	22.486106825548806	22.36119814778881	28.014005040881614	27.13868998578077
45	21.30954766729333	20.82274313452919	26.573631968987023	31.29407722919046
46	22.329069684833563	22.53042925959268	28.026199011970238	27.114302043603523
47	17.84571763640547	24.950561399825467	32.43787171730346	24.765849246465603
48	19.31959762016098	26.589537148667837	27.57512811622233	26.515737114948855
49	22.069179048849048	23.376160680487207	29.86886709525824	24.6857931754055
50	21.294278694799747	23.203430429153556	27.077826164868853	28.424464711177844
51	19.829835784321524	23.50212970355926	25.59981083439633	31.06822367772289
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	655.0
2	1134.0
3	2117.0
4	3100.0
5	2158.5
6	1217.0
7	1230.0
8	1243.0
9	1328.0
10	1413.0
11	1463.5
12	1514.0
13	1493.5
14	1473.0
15	1457.5
16	1442.0
17	1515.5
18	1589.0
19	1753.0
20	1917.0
21	2085.0
22	2253.0
23	2614.0
24	2975.0
25	3948.5
26	5800.0
27	6678.0
28	8251.5
29	9825.0
30	11259.5
31	12694.0
32	14277.5
33	15861.0
34	17719.0
35	19577.0
36	21317.5
37	23058.0
38	25467.0
39	27876.0
40	31054.5
41	34233.0
42	39108.5
43	43984.0
44	51025.5
45	58067.0
46	76240.0
47	94413.0
48	100699.5
49	106986.0
50	104302.0
51	101618.0
52	90023.0
53	78428.0
54	72096.0
55	65764.0
56	60070.5
57	54377.0
58	49004.0
59	43631.0
60	38303.0
61	32975.0
62	29168.0
63	25361.0
64	22268.5
65	19176.0
66	16321.5
67	13467.0
68	11592.5
69	9718.0
70	8363.0
71	7008.0
72	5966.0
73	4924.0
74	4170.0
75	2644.5
76	1873.0
77	1417.5
78	962.0
79	707.5
80	453.0
81	343.5
82	234.0
83	149.5
84	65.0
85	41.0
86	17.0
87	8.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	943089.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.48338541945365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.53449412926786	28.639073879109812
2	10.58447509581206	8.358218193411124
3	4.053220454460521	4.801045961804334
4	2.402572324281192	3.794467563108275
5	1.7246938827605547	3.40483766518045
6	1.3072471826024041	3.0968726609511372
7	1.0306007345324686	2.848412421058225
8	0.8681165284535455	2.7420943585543553
9	0.7181810366629771	2.552059680435639
>10	4.647406326892243	30.444863467279326
>50	0.08034246829394981	2.21109084568872
>100	0.04197785847536967	3.1238675422537607
>500	0.0036139878151597404	1.064595618042813
>1k	0.0030579896897505498	2.9185001431220083
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGC	4405	0.46708210995992955	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCG	3945	0.4183062256054307	No Hit
CCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	3492	0.37027258296936977	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC	2995	0.3175734209602699	No Hit
CTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGCT	2466	0.2614811539525962	No Hit
GCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	2393	0.25374063317459966	No Hit
TCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	1851	0.1962699172612553	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCCATCAAT	1686	0.17877421961235895	No Hit
CCTGTATCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTCTGC	1323	0.14028368478478703	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTC	1029	0.10910953261038989	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1004	0.1064586693302541	No Hit
GAATCAGTCTCTTATACACATCTGACGCGCCATCAATCGTATGCCGTCTTC	991	0.10508022042458347	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0603453120543237E-4	0.0	0.0	0.28236995660006636	0.0
2	1.0603453120543237E-4	0.0	0.0	1.1858901970015556	0.0
3	1.0603453120543237E-4	0.0	0.0	1.6398240250920115	0.0
4	1.0603453120543237E-4	0.0	0.0	2.1440182209738423	0.0
5	1.0603453120543237E-4	0.0	0.0	3.255896315194006	0.0
6	1.0603453120543237E-4	0.0	0.0	3.9331388660031026	0.0
7	1.0603453120543237E-4	0.0	0.0	4.833478070468429	0.0
8	1.0603453120543237E-4	0.0	0.0	6.1630450572533455	0.0
9	1.0603453120543237E-4	0.0	0.0	6.744114288259114	0.0
10	1.0603453120543237E-4	0.0	0.0	8.224992551074182	0.0
11	1.0603453120543237E-4	0.0	0.0	10.119617554652848	0.0
12	2.1206906241086473E-4	0.0	0.0	12.261515085002582	0.0
13	2.1206906241086473E-4	0.0	0.0	12.877469676774938	0.0
14	2.1206906241086473E-4	0.0	0.0	13.140753417758027	0.0
15	2.1206906241086473E-4	0.0	0.0	13.557999298051403	0.0
16	2.1206906241086473E-4	0.0	0.0	14.260478067287393	0.0
17	2.1206906241086473E-4	0.0	0.0	15.19538452892569	0.0
18	2.1206906241086473E-4	0.0	0.0	16.121065986349116	0.0
19	2.1206906241086473E-4	0.0	0.0	17.019920707377565	0.0
20	2.1206906241086473E-4	0.0	0.0	17.757390871911348	0.0
21	2.1206906241086473E-4	0.0	0.0	18.703430959326216	0.0
22	2.1206906241086473E-4	0.0	0.0	19.694747791565803	0.0
23	2.1206906241086473E-4	0.0	0.0	20.596041306811976	0.0
24	2.1206906241086473E-4	0.0	0.0	21.32863388291031	0.0
25	2.1206906241086473E-4	0.0	0.0	21.963144517643617	0.0
26	2.1206906241086473E-4	0.0	0.0	22.545168059430235	0.0
27	2.1206906241086473E-4	0.0	0.0	23.277866670059773	0.0
28	2.1206906241086473E-4	0.0	0.0	23.921284205414334	0.0
29	2.1206906241086473E-4	0.0	0.0	24.682718174000545	0.0
30	2.1206906241086473E-4	0.0	0.0	25.4470150749293	0.0
31	2.1206906241086473E-4	0.0	0.0	26.07177053279171	0.0
32	2.1206906241086473E-4	0.0	0.0	26.67065356503999	0.0
33	2.1206906241086473E-4	0.0	0.0	27.266461595883314	0.0
34	2.1206906241086473E-4	0.0	0.0	27.977105024022123	0.0
35	2.1206906241086473E-4	0.0	0.0	28.717650189960864	0.0
36	2.1206906241086473E-4	0.0	0.0	29.346434960009077	0.0
37	2.1206906241086473E-4	0.0	0.0	30.014558541134505	0.0
38	2.1206906241086473E-4	0.0	0.0	30.81215028486177	0.0
39	2.1206906241086473E-4	0.0	0.0	32.27807767877687	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGCGG	20	7.032641E-4	45.000004	1
ATTGCGA	20	7.032641E-4	45.000004	11
GGTACGT	25	3.8902712E-5	45.0	25
TCGTTAG	100	0.0	45.0	1
AACGTAG	30	2.1650867E-6	44.999996	36
ATTACGG	30	2.1650867E-6	44.999996	2
CATACGA	110	0.0	42.954544	18
ACATACG	110	0.0	42.954544	17
TAATACG	175	0.0	42.42857	4
AATACGG	160	0.0	42.187504	5
CGTTTAT	325	0.0	40.846153	1
CGTTAGG	215	0.0	40.813953	2
CGATGAA	425	0.0	40.764706	19
TAGGGCG	345	0.0	39.782608	5
AGGGCGA	510	0.0	39.705883	6
ACGGGAG	410	0.0	39.512196	5
CTAGGGA	1065	0.0	39.507042	4
TATGGGA	1220	0.0	39.467213	4
CGCACGG	120	0.0	39.374996	2
CACGGGA	315	0.0	39.285713	4
>>END_MODULE