FastQCFastQC Report
Sat 14 Jan 2017
SRR2934157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401035
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC41161.0263443340356826No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG35240.8787262957098507No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC25120.6263792437069083No Hit
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC21710.5413492587928734TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT20080.5007044272943758TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC18410.45906217661800097TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC15120.3770244492375977TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTAGCACT14640.3650554191030708No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC10320.25733414789232856No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT9900.24686124652461755No Hit
GAATCAGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC9450.23564028077349858No Hit
CCTGTATCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC8030.20023189995885646TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC7630.19025770818008403TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC6780.16906255065019263TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCACTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT6740.1680651314723154TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA6580.1640754547608064No Hit
TCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG5610.13988803969728328No Hit
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT5270.1314099766853267No Hit
GAATATGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC4990.12442804244018602No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC4630.11545126983929083No Hit
CGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG4390.10946675477202737No Hit
GCTGACTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC4090.10198611093794807TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGAT207.027787E-445.021
TCGCAAT253.8862483E-545.022
CATGCGG253.8862483E-545.02
ACCGCAT207.027787E-445.034
CGCATGG253.8862483E-545.02
CGCATAT207.027787E-445.036
TCTTACG551.8189894E-1245.01
CCTACGG207.027787E-445.02
CTAAGAC207.027787E-445.036
ACGGGTA502.1827873E-1145.05
CCGCTAG207.027787E-445.01
ACGGGAC700.045.05
ATCGTAT406.7957444E-945.033
GCTTACG207.027787E-445.01
CCATAGC351.2092823E-745.035
TTAACGG207.027787E-445.02
CATAAGT207.027787E-445.018
CCGGTAC207.027787E-445.011
TTGCCAA207.027787E-445.01
GACGCAT207.027787E-445.029
Produced by FastQC (version 0.11.5)