##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934157.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	401035
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.777874749086738	31.0	31.0	34.0	30.0	34.0
2	31.913286870223295	33.0	31.0	34.0	30.0	34.0
3	32.00154101262982	33.0	31.0	34.0	30.0	34.0
4	35.58164499357911	37.0	35.0	37.0	33.0	37.0
5	29.488999713241988	35.0	35.0	37.0	0.0	37.0
6	23.653164935728803	35.0	10.0	37.0	0.0	37.0
7	30.31955315620831	35.0	19.0	37.0	17.0	37.0
8	33.953181143790445	35.0	32.0	37.0	32.0	37.0
9	36.72222125250913	37.0	35.0	39.0	34.0	39.0
10	36.87359956113556	39.0	37.0	39.0	33.0	39.0
11	37.04258730534741	39.0	37.0	39.0	34.0	39.0
12	37.03531611954069	39.0	37.0	39.0	34.0	39.0
13	36.9077187776628	39.0	37.0	39.0	33.0	39.0
14	37.98780655055045	40.0	37.0	41.0	33.0	41.0
15	37.843278018128096	40.0	37.0	41.0	33.0	41.0
16	38.13825476579351	40.0	37.0	41.0	34.0	41.0
17	38.11297018963432	40.0	37.0	41.0	34.0	41.0
18	38.209201191915916	40.0	37.0	41.0	34.0	41.0
19	38.22888276584338	40.0	37.0	41.0	34.0	41.0
20	38.10159961100652	40.0	37.0	41.0	34.0	41.0
21	38.04063735085467	40.0	37.0	41.0	34.0	41.0
22	38.11392771204508	40.0	37.0	41.0	34.0	41.0
23	38.04307604074457	40.0	37.0	41.0	34.0	41.0
24	38.01812061291408	40.0	37.0	41.0	34.0	41.0
25	37.79378857206977	40.0	36.0	41.0	33.0	41.0
26	37.642003815128355	39.0	36.0	41.0	33.0	41.0
27	36.789801388906206	39.0	36.0	41.0	32.0	41.0
28	37.18711085067388	39.0	36.0	41.0	32.0	41.0
29	37.423748550625255	39.0	36.0	41.0	32.0	41.0
30	37.48884760681736	39.0	36.0	41.0	33.0	41.0
31	37.498694627650956	39.0	36.0	41.0	33.0	41.0
32	37.37241886618375	39.0	36.0	41.0	33.0	41.0
33	36.29876195344546	39.0	35.0	41.0	31.0	41.0
34	36.71231438652487	39.0	35.0	41.0	31.0	41.0
35	37.004972134601715	39.0	35.0	41.0	31.0	41.0
36	37.190050743700674	39.0	35.0	41.0	32.0	41.0
37	37.28839378109143	39.0	35.0	41.0	33.0	41.0
38	37.197820639096335	39.0	35.0	41.0	32.0	41.0
39	37.224671163364796	39.0	35.0	41.0	33.0	41.0
40	37.12371987482389	39.0	35.0	40.0	32.0	41.0
41	37.071933871108506	39.0	35.0	40.0	32.0	41.0
42	37.14219706509407	39.0	35.0	40.0	33.0	41.0
43	37.10169685937636	39.0	35.0	40.0	33.0	41.0
44	37.16774346378745	39.0	35.0	40.0	33.0	41.0
45	37.100482501527296	39.0	35.0	40.0	33.0	41.0
46	37.03748799980052	39.0	35.0	40.0	32.0	41.0
47	36.91329933796302	39.0	35.0	40.0	32.0	41.0
48	36.9298963930829	39.0	35.0	40.0	32.0	41.0
49	37.02318002169387	39.0	35.0	40.0	33.0	41.0
50	36.92376725223484	39.0	35.0	40.0	32.0	41.0
51	36.21177952049073	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	5.0
12	7.0
13	7.0
14	4.0
15	15.0
16	22.0
17	39.0
18	66.0
19	116.0
20	200.0
21	404.0
22	689.0
23	991.0
24	1391.0
25	1753.0
26	2135.0
27	2501.0
28	3276.0
29	4536.0
30	6508.0
31	9060.0
32	13373.0
33	21466.0
34	29308.0
35	39505.0
36	48820.0
37	71823.0
38	79345.0
39	63627.0
40	36.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.531013502562118	4.4215592155298165	51.1469073771616	16.90051990474647
2	17.889710374406224	10.746692932038352	50.33201590883589	21.031580784719537
3	19.59429974939843	10.49409652524094	49.47149251312229	20.44011121223833
4	18.46970962634184	3.944543493710025	53.35519343698181	24.230553442966325
5	17.71042427718279	21.7257845325221	39.38808333437231	21.1757078559228
6	15.175732791402247	32.436071664567926	38.168987744211854	14.219207799817971
7	75.99062425972795	1.9377361078210131	15.801613325520217	6.270026306930816
8	75.45924919271386	6.277506950764895	10.198611093794806	8.064632762726445
9	70.60456069919084	4.701335294924383	12.545538419339957	12.148565586544816
10	39.25542658371464	23.813632226613638	19.678581669929056	17.252359519742665
11	28.156644681885624	22.548904709065294	27.94294762302542	21.35150298602366
12	23.514655828044933	18.999089855000186	33.25021506851023	24.236039248444648
13	21.64648970788086	24.397870510055235	34.9006944531026	19.054945328961313
14	17.99294325931652	29.392197688481055	30.526263293727478	22.088595758474945
15	14.609946762751381	23.308190058224344	40.23539092597903	21.846472253045246
16	16.168663582979043	23.47949680202476	32.492425848117996	27.859413766878205
17	16.51576545688032	23.959504781378186	30.826735821063	28.697993940678497
18	17.692470731981	22.22848379817223	34.088795242310525	25.99025022753625
19	19.643173289114415	23.980699938908074	29.865223733589335	26.51090303838817
20	22.443926340593716	25.081102646901144	32.15829042352912	20.316680588976023
21	22.1377186529854	28.070866632588178	28.719438453002855	21.07197626142357
22	19.209295946737818	23.87821511838119	29.33933447205356	27.573154462827432
23	18.891617938583913	26.327128554864288	27.667410575136834	27.113842931414965
24	21.404615557245627	23.043873976086875	29.253556422756116	26.297954043911382
25	18.680414427668406	28.165621454486516	26.548804967147504	26.60515915069757
26	17.71017492238832	27.810789581956683	28.28830401336542	26.190731482289575
27	19.49430847681624	28.560848803720372	26.995150049247574	24.949692670215818
28	15.989377485755607	25.381076464647723	32.88790255214632	25.741643497450344
29	18.16524742229481	25.584300622140212	29.573977333649182	26.67647462191579
30	21.469447803807647	26.471754335656488	27.08641390402334	24.972383956512523
31	20.970987569663496	26.79541685887765	24.737491740122433	27.496103831336416
32	21.202638173725486	31.836622738663706	22.05094318451008	24.909795903100726
33	21.522560375029613	26.849526849277495	22.429962472103433	29.197950303589465
34	18.470457690725247	26.53982819454661	26.458787886344087	28.530926228384057
35	18.211378059271635	27.862156669617367	23.691448377323677	30.235016893787325
36	20.50145249167778	26.715872679441944	25.288067126310672	27.494607702569603
37	16.727467677384766	26.35156532472228	29.257296744673155	27.663670253219795
38	19.81672422606506	27.504083184759438	26.508908200032415	26.170284389143095
39	22.00406448314985	26.869724587629506	25.848367349483215	25.27784357973743
40	22.99874075828793	24.41632276484596	26.29072275487177	26.294213721994343
41	17.733115563479494	26.086251823406936	23.881706085503758	32.29892652760981
42	19.281858191928386	25.58878900844066	26.79815976161682	28.331193038014142
43	21.393643946288975	22.761853703542084	26.795915568466594	29.048586781702348
44	20.046878701360228	22.817210467914272	28.802722954355602	28.33318787636989
45	20.169062550650192	22.83167304599349	26.650791078085454	30.348473325270863
46	23.916615756729463	24.252496664879626	27.28564838480432	24.545239193586596
47	17.12119889785181	24.02259154437892	32.44654456593564	26.40966499183363
48	17.95952971685763	24.349994389517125	28.13544952435573	29.555026369269516
49	20.27503833829965	20.305210268430436	32.886157068585035	26.533594324684877
50	21.089929806625356	20.19200319174137	28.781278442031244	29.93678855960203
51	18.90084406597928	21.086438839502787	25.99349183986435	34.019225254653584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	185.5
2	294.0
3	627.0
4	960.0
5	685.5
6	411.0
7	446.5
8	482.0
9	597.0
10	712.0
11	772.5
12	833.0
13	877.5
14	922.0
15	886.0
16	850.0
17	883.5
18	917.0
19	858.0
20	799.0
21	954.0
22	1109.0
23	1188.5
24	1268.0
25	1479.5
26	2068.0
27	2445.0
28	2824.0
29	3203.0
30	3851.5
31	4500.0
32	5197.5
33	5895.0
34	7075.5
35	8256.0
36	9241.0
37	10226.0
38	11307.5
39	12389.0
40	14987.0
41	17585.0
42	20792.5
43	24000.0
44	28759.5
45	33519.0
46	46268.5
47	59018.0
48	57887.5
49	56757.0
50	51224.0
51	45691.0
52	36744.0
53	27797.0
54	23336.5
55	18876.0
56	16713.5
57	14551.0
58	13465.0
59	12379.0
60	10963.5
61	9548.0
62	8265.0
63	6982.0
64	6280.5
65	5579.0
66	4586.5
67	3594.0
68	2844.5
69	2095.0
70	1914.0
71	1733.0
72	1476.5
73	1220.0
74	1123.0
75	685.5
76	345.0
77	282.0
78	219.0
79	204.0
80	189.0
81	128.5
82	68.0
83	40.0
84	12.0
85	12.0
86	12.0
87	6.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	401035.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.18053327121303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.62035868612074	30.317256303124324
2	11.0015464294735	9.060990975474567
3	4.144144441507567	5.119742341626448
4	2.3471543382921554	3.8662826928284844
5	1.577986662392834	3.249116612609925
6	1.1570053530409579	2.858765846152485
7	0.8949949248035626	2.579945779490794
8	0.7413373292497114	2.4422933241887956
9	0.5968730166835513	2.212159421000365
>10	3.788325513248738	25.851374018065464
>50	0.07568258682277008	2.146145134493422
>100	0.04342443506224513	3.45488237101405
>500	0.005583141650860088	1.6858007073588965
>1k	0.005583141650860088	5.155244472571986
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	4116	1.0263443340356826	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCG	3524	0.8787262957098507	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	2512	0.6263792437069083	No Hit
CCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	2171	0.5413492587928734	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	2008	0.5007044272943758	TruSeq Adapter, Index 13 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	1841	0.45906217661800097	TruSeq Adapter, Index 16 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	1512	0.3770244492375977	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTAGCACT	1464	0.3650554191030708	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTC	1032	0.25733414789232856	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCT	990	0.24686124652461755	No Hit
GAATCAGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	945	0.23564028077349858	No Hit
CCTGTATCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	803	0.20023189995885646	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	763	0.19025770818008403	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	678	0.16906255065019263	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCACTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGCT	674	0.1680651314723154	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTAGCACTCGTA	658	0.1640754547608064	No Hit
TCCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG	561	0.13988803969728328	No Hit
GAACTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCT	527	0.1314099766853267	No Hit
GAATATGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTC	499	0.12442804244018602	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGC	463	0.11545126983929083	No Hit
CGCTGTCTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTG	439	0.10946675477202737	No Hit
GCTGACTCTTATACACATCTGACGCACTAGCACTCGTATGCCGTCTTCTGC	409	0.10198611093794807	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5333699053698555	0.0
2	0.0	0.0	0.0	2.040470283142369	0.0
3	0.0	0.0	0.0	2.7573653172416375	0.0
4	0.0	0.0	0.0	3.6188861321331056	0.0
5	0.0	0.0	0.0	5.49104192900869	0.0
6	0.0	0.0	0.0	6.462528208261125	0.0
7	0.0	0.0	0.0	7.896068921665191	0.0
8	0.0	0.0	0.0	9.984415325345669	0.0
9	0.0	0.0	0.0	10.787836473125788	0.0
10	0.0	0.0	0.0	13.3040757041156	0.0
11	0.0	0.0	0.0	15.965688780281022	0.0
12	0.0	0.0	0.0	19.585821686386474	0.0
13	0.0	0.0	0.0	20.458314112234593	0.0
14	0.0	0.0	0.0	20.783472764222573	0.0
15	0.0	0.0	0.0	21.40287007368434	0.0
16	0.0	0.0	0.0	22.473100851546622	0.0
17	0.0	0.0	0.0	23.874973506053088	0.0
18	0.0	0.0	0.0	25.493784831747853	0.0
19	0.0	0.0	0.0	26.950016831448625	0.0
20	0.0	0.0	0.0	27.971623424389392	0.0
21	0.0	0.0	0.0	29.146333861134316	0.0
22	0.0	0.0	0.0	30.347974615681924	0.0
23	0.0	0.0	0.0	31.56382859351428	0.0
24	0.0	0.0	0.0	32.51636390838705	0.0
25	0.0	0.0	0.0	33.270911516451186	0.0
26	0.0	0.0	0.0	33.961374942336704	0.0
27	0.0	0.0	0.0	34.69896642437693	0.0
28	0.0	0.0	0.0	35.38394404478412	0.0
29	0.0	0.0	0.0	36.13300584736993	0.0
30	0.0	0.0	0.0	36.9538818307629	0.0
31	0.0	0.0	0.0	37.68100041143541	0.0
32	0.0	0.0	0.0	38.373957385265626	0.0
33	0.0	0.0	0.0	39.02078372211902	0.0
34	2.493547944693107E-4	0.0	0.0	39.76211552607628	0.0
35	2.493547944693107E-4	0.0	0.0	40.47851185058661	0.0
36	2.493547944693107E-4	0.0	0.0	41.09865722443178	0.0
37	2.493547944693107E-4	0.0	0.0	41.734511950328525	0.0
38	2.493547944693107E-4	0.0	0.0	42.415749248818685	0.0
39	2.493547944693107E-4	0.0	0.0	43.10346977196504	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGAT	20	7.027787E-4	45.0	21
TCGCAAT	25	3.8862483E-5	45.0	22
CATGCGG	25	3.8862483E-5	45.0	2
ACCGCAT	20	7.027787E-4	45.0	34
CGCATGG	25	3.8862483E-5	45.0	2
CGCATAT	20	7.027787E-4	45.0	36
TCTTACG	55	1.8189894E-12	45.0	1
CCTACGG	20	7.027787E-4	45.0	2
CTAAGAC	20	7.027787E-4	45.0	36
ACGGGTA	50	2.1827873E-11	45.0	5
CCGCTAG	20	7.027787E-4	45.0	1
ACGGGAC	70	0.0	45.0	5
ATCGTAT	40	6.7957444E-9	45.0	33
GCTTACG	20	7.027787E-4	45.0	1
CCATAGC	35	1.2092823E-7	45.0	35
TTAACGG	20	7.027787E-4	45.0	2
CATAAGT	20	7.027787E-4	45.0	18
CCGGTAC	20	7.027787E-4	45.0	11
TTGCCAA	20	7.027787E-4	45.0	1
GACGCAT	20	7.027787E-4	45.0	29
>>END_MODULE