##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934156.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	916252
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03603812051706	33.0	31.0	34.0	30.0	34.0
2	32.23102705369265	33.0	31.0	34.0	30.0	34.0
3	32.30427546133596	34.0	31.0	34.0	30.0	34.0
4	35.74253589623815	37.0	35.0	37.0	35.0	37.0
5	29.967063646245794	37.0	35.0	37.0	0.0	37.0
6	24.149583302410253	35.0	17.0	37.0	0.0	37.0
7	30.59600524746467	35.0	19.0	37.0	17.0	37.0
8	34.308860444506536	35.0	32.0	37.0	32.0	37.0
9	36.978926103299095	38.0	37.0	39.0	34.0	39.0
10	37.103579582909504	39.0	37.0	39.0	34.0	39.0
11	37.23025106630054	39.0	37.0	39.0	34.0	39.0
12	36.98131845824074	39.0	35.0	39.0	33.0	39.0
13	36.80478405504163	39.0	35.0	39.0	33.0	39.0
14	37.55463562426058	40.0	36.0	41.0	32.0	41.0
15	37.512575143082906	40.0	36.0	41.0	32.0	41.0
16	37.89680895648795	40.0	36.0	41.0	33.0	41.0
17	37.943176113121716	40.0	36.0	41.0	33.0	41.0
18	38.04230495540528	40.0	36.0	41.0	33.0	41.0
19	38.04565665340976	40.0	36.0	41.0	34.0	41.0
20	37.94882303121849	40.0	36.0	41.0	33.0	41.0
21	37.8601869354719	40.0	36.0	41.0	33.0	41.0
22	37.999883219900205	40.0	36.0	41.0	34.0	41.0
23	37.94708006094393	40.0	36.0	41.0	34.0	41.0
24	37.92679852267717	40.0	35.0	41.0	34.0	41.0
25	37.71478043158432	40.0	35.0	41.0	33.0	41.0
26	37.617450221118204	40.0	35.0	41.0	33.0	41.0
27	36.81379140236529	39.0	35.0	41.0	32.0	41.0
28	37.13984144100095	39.0	35.0	41.0	33.0	41.0
29	37.38618851582316	39.0	35.0	41.0	32.0	41.0
30	37.36980983397581	39.0	35.0	41.0	33.0	41.0
31	37.373174628813906	39.0	35.0	41.0	33.0	41.0
32	37.36486687068623	39.0	35.0	41.0	33.0	41.0
33	36.25818442961107	39.0	35.0	41.0	31.0	41.0
34	36.61815745013381	39.0	35.0	41.0	31.0	41.0
35	36.85075939806953	39.0	35.0	41.0	31.0	41.0
36	36.94991552542314	39.0	35.0	41.0	32.0	41.0
37	37.03125668484216	39.0	35.0	41.0	32.0	41.0
38	37.001485399213315	39.0	35.0	41.0	32.0	41.0
39	37.007935589772245	39.0	35.0	41.0	32.0	41.0
40	36.93022225326657	39.0	35.0	41.0	32.0	41.0
41	36.819584568437506	39.0	35.0	41.0	32.0	41.0
42	36.89056067544737	39.0	35.0	41.0	32.0	41.0
43	36.787425293478215	39.0	35.0	41.0	32.0	41.0
44	36.8146830784544	39.0	35.0	41.0	32.0	41.0
45	36.75878797536049	39.0	35.0	40.0	32.0	41.0
46	36.713957513871726	39.0	35.0	40.0	32.0	41.0
47	36.654548093755864	38.0	35.0	40.0	32.0	41.0
48	36.60648707997363	38.0	35.0	40.0	32.0	41.0
49	36.62172088028184	38.0	35.0	40.0	32.0	41.0
50	36.518373766169134	38.0	35.0	40.0	32.0	41.0
51	35.77515356037422	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	3.0
9	5.0
10	8.0
11	9.0
12	10.0
13	16.0
14	23.0
15	24.0
16	43.0
17	85.0
18	182.0
19	331.0
20	670.0
21	1130.0
22	1746.0
23	2540.0
24	3388.0
25	4588.0
26	5872.0
27	6969.0
28	8591.0
29	11335.0
30	15836.0
31	22322.0
32	33238.0
33	56751.0
34	75289.0
35	80535.0
36	97398.0
37	146124.0
38	176271.0
39	164806.0
40	112.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.72037823655501	4.398244151172385	52.90367715431999	15.977700457952615
2	20.442847600878363	6.802604523646333	51.43290273854792	21.321645136927394
3	21.101509191794396	6.8961377437648155	50.54908474961037	21.453268314830417
4	18.9516639527117	4.471040718055732	51.56561731925278	25.01167800997979
5	16.81906287789822	21.184019243614202	41.00640435164125	20.99051352684633
6	17.360289527335272	30.36075228212326	38.80460833919053	13.474349851350938
7	82.48669579984545	1.9228334562980491	10.922322679786783	4.66814806406971
8	84.37591404984654	3.8614922532229126	7.163422289937703	4.599171406992836
9	79.60495584184264	4.381763968864461	8.910649035418205	7.1026311538746985
10	44.440175846819436	25.1665480675622	15.718383152233228	14.67489293338514
11	36.816072434221155	21.76824716344412	23.192855240697973	18.222825161636756
12	30.951310338203903	20.35116976552302	27.98487752277758	20.712642373495502
13	28.82940501084854	21.128685121560444	31.097994874772443	18.94391499281857
14	22.205353985584754	23.472690919092127	33.6145514552765	20.707403640046625
15	20.06478567031777	20.860854873986632	37.727066352924744	21.347293102770852
16	26.40059721561317	21.53566922637004	29.80904816578845	22.25468539222834
17	27.300131404897343	21.624072853319827	28.343730764025615	22.73206497775721
18	28.197810209418368	19.888305837258745	29.44397392856987	22.469910024753016
19	27.20135945132998	23.014629163155988	27.756010355229783	22.028001030284244
20	26.59912338526955	27.92736059512012	27.35437412414925	18.119141895461073
21	25.08392887546221	25.690203131889476	30.227164579176907	18.9987034134714
22	23.40567878705858	21.672749418282304	32.26219424350506	22.65937755115405
23	22.327263678551315	24.09075232577937	31.220122848299376	22.36186114736994
24	23.3338644826969	25.83514142397506	28.184495095235807	22.646498998092227
25	24.874925238908073	25.705155350274815	25.310394956845933	24.10952445397118
26	21.966882473380686	23.61315445969013	27.87224475362673	26.547718313302454
27	21.81583232560475	25.19994499329879	29.638789328699964	23.3454333523965
28	19.89921986527724	25.191432051444362	28.850469084924235	26.058878998354164
29	22.80999113780925	25.909902515901738	27.768234066610496	23.511872279678517
30	25.90302667825009	25.265210880849377	28.360756647734465	20.47100579316607
31	25.891894369671224	23.725568948280603	28.57947376922506	21.80306291282311
32	28.600428703020565	24.226850255169975	25.874868485962377	21.297852555847083
33	27.480540288043027	25.782099247805192	24.436945294525962	22.300415169625822
34	23.381777065698085	23.365406023670342	31.07758564237786	22.17523126825371
35	22.413047938776668	26.823515801329766	28.07950214569791	22.683934114195658
36	26.144554118299336	28.007687841336228	25.829356989125262	20.01840105123918
37	24.35247071766283	28.074809113649955	26.155686426878194	21.41703374180902
38	26.70848194601485	25.78166268668445	24.236127178985694	23.273728188315005
39	24.97806280368283	24.148814954837754	26.144008416898405	24.729113824581013
40	25.119399466522314	23.634218533765818	27.922449282511796	23.323932717200073
41	22.088683026067066	23.202677865914616	26.947171738779286	27.76146736923903
42	22.606990216665285	23.0427873554437	29.399226413694052	24.950996014196967
43	24.293753246923337	22.095886284559267	29.028586022186037	24.58177444633136
44	24.30783234306719	22.528409214932136	28.246705054941216	24.917053387059454
45	22.822105708909778	21.64841113580107	26.724962128322776	28.804521026966377
46	23.34270484539188	22.82614389927662	28.177728397864342	25.653422857467163
47	19.75089822450592	25.039290500866574	32.34088438551839	22.868926889109108
48	21.75766055626618	26.184281180286646	27.942640234346012	24.115418029101164
49	24.587231460340604	22.742433304374778	29.88151731183124	22.788817923453372
50	23.013646900634324	23.47149037601009	27.439721823253866	26.07514090010172
51	20.665057211334872	23.881530408664865	26.577077048672198	28.87633533132806
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	609.0
2	1047.0
3	2071.5
4	3096.0
5	2209.0
6	1322.0
7	1344.0
8	1366.0
9	1424.5
10	1483.0
11	1520.5
12	1558.0
13	1598.5
14	1639.0
15	1617.0
16	1595.0
17	1625.0
18	1655.0
19	1577.0
20	1499.0
21	1849.0
22	2199.0
23	2945.0
24	3691.0
25	4251.5
26	5714.5
27	6617.0
28	8183.0
29	9749.0
30	11054.0
31	12359.0
32	13889.5
33	15420.0
34	17420.5
35	19421.0
36	21332.0
37	23243.0
38	25644.5
39	28046.0
40	31244.0
41	34442.0
42	39686.5
43	44931.0
44	50648.5
45	56366.0
46	70936.0
47	85506.0
48	91051.0
49	96596.0
50	94619.5
51	92643.0
52	83721.5
53	74800.0
54	69565.5
55	64331.0
56	59532.5
57	54734.0
58	50347.5
59	45961.0
60	40637.5
61	35314.0
62	31540.0
63	27766.0
64	24328.0
65	20890.0
66	17308.0
67	13726.0
68	11179.0
69	8632.0
70	7704.5
71	6777.0
72	5639.0
73	4501.0
74	3737.5
75	2368.5
76	1763.0
77	1391.0
78	1019.0
79	705.0
80	391.0
81	253.5
82	116.0
83	80.0
84	44.0
85	29.0
86	14.0
87	13.0
88	12.0
89	11.0
90	10.0
91	7.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	916252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.83809216889716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.16506622374534	28.027534933723892
2	10.006803779810967	7.772903350327337
3	3.99707341438719	4.657161170214544
4	2.4948171659600207	3.875757561444083
5	1.7611096124545196	3.41990687240197
6	1.3939914156760456	3.2483980250806614
7	1.1469894198252437	3.1182816562745885
8	0.9408220281974498	2.9231786116529057
9	0.7899653844702673	2.761267357106507
>10	5.189238277794658	32.88217012067318
>50	0.07275741487504232	1.9431627930535822
>100	0.03579173944706858	2.607772686170676
>500	0.00322712404828146	0.838493432812777
>1k	0.002346999307841062	1.9240114290632628
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGC	3536	0.3859200307339029	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCG	2980	0.32523803495108333	No Hit
CCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	2230	0.24338282481238785	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTC	2207	0.24087259836813452	No Hit
CTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGCT	1904	0.20780309347210157	No Hit
GCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	1584	0.17287820381292485	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGGATCGT	1315	0.1435194684431794	No Hit
TCTGTCTCTTATACACATCTGACGCTAGGATCGTCGTATGCCGTCTTCTGC	1130	0.12332851660896785	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	937	0.10226444253327686	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22024508541318327	0.0
2	0.0	0.0	0.0	0.8062192497260579	0.0
3	0.0	0.0	0.0	1.115304523209772	0.0
4	0.0	0.0	0.0	1.5006788525427504	0.0
5	0.0	0.0	0.0	2.360922540960347	0.0
6	0.0	0.0	0.0	2.8971287375088948	0.0
7	0.0	0.0	0.0	3.623020741018846	0.0
8	0.0	0.0	0.0	4.669348607151744	0.0
9	0.0	0.0	0.0	5.173140140485368	0.0
10	0.0	0.0	0.0	6.426288837568704	0.0
11	0.0	0.0	0.0	7.948686605868255	0.0
12	0.0	0.0	0.0	9.70595425712577	0.0
13	0.0	0.0	0.0	10.193483888711839	0.0
14	0.0	0.0	0.0	10.41634834084946	0.0
15	0.0	0.0	0.0	10.759048820630133	0.0
16	0.0	0.0	0.0	11.315445969012892	0.0
17	0.0	0.0	0.0	12.035662677953226	0.0
18	0.0	0.0	0.0	12.800626901769382	0.0
19	0.0	0.0	0.0	13.531102797047101	0.0
20	0.0	0.0	0.0	14.146981398130645	0.0
21	0.0	0.0	0.0	14.977102369217203	0.0
22	0.0	0.0	0.0	15.818137368322253	0.0
23	0.0	0.0	0.0	16.57873598093101	0.0
24	0.0	0.0	0.0	17.1805354858707	0.0
25	0.0	0.0	0.0	17.705391093280014	0.0
26	0.0	0.0	0.0	18.209400907174008	0.0
27	0.0	0.0	0.0	18.842741953087142	0.0
28	0.0	0.0	0.0	19.381021814959205	0.0
29	0.0	0.0	0.0	20.027241413934156	0.0
30	0.0	0.0	0.0	20.69703531342906	0.0
31	0.0	0.0	0.0	21.210867752539695	0.0
32	0.0	0.0	0.0	21.718260915119423	0.0
33	0.0	0.0	0.0	22.241261137765594	0.0
34	0.0	0.0	0.0	22.880823179649266	0.0
35	0.0	0.0	0.0	23.528243321706256	0.0
36	0.0	0.0	0.0	24.08518617148994	0.0
37	0.0	0.0	0.0	24.683274906903343	0.0
38	0.0	0.0	0.0	25.462863928264277	0.0
39	0.0	0.0	0.0	26.967035269772946	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	30	2.1650176E-6	45.000004	1
AACCCGA	30	2.1650176E-6	45.000004	24
AACGGTT	20	7.032535E-4	45.000004	40
ACCCGTC	30	2.1650176E-6	45.000004	39
TACCGAA	20	7.032535E-4	45.000004	23
ACGGTAT	20	7.032535E-4	45.000004	19
TATTACG	25	3.8901835E-5	45.0	1
CGATCGA	25	3.8901835E-5	45.0	41
TCGTTAG	110	0.0	42.954544	1
TACGGGT	95	0.0	42.63158	4
TAATACG	170	0.0	42.35294	4
AATACGG	175	0.0	41.142857	5
GTCGAAT	55	6.002665E-11	40.909092	43
ATACGGA	55	6.002665E-11	40.909092	4
CGTTTAT	195	0.0	40.384617	1
TATGGGA	1250	0.0	40.32	4
AGACACG	330	0.0	40.227276	24
ACACGTG	325	0.0	40.153847	42
TTATGGG	2250	0.0	40.1	3
CGATGAA	485	0.0	39.896908	19
>>END_MODULE