##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934155.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 296352 Sequences flagged as poor quality 0 Sequence length 51 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69176182377713 31.0 31.0 34.0 30.0 34.0 2 31.755432728647015 31.0 31.0 34.0 30.0 34.0 3 31.833792247057552 33.0 31.0 34.0 30.0 34.0 4 35.5524275186265 37.0 35.0 37.0 33.0 37.0 5 29.75811872367995 35.0 35.0 37.0 0.0 37.0 6 24.046977918151388 35.0 17.0 37.0 0.0 37.0 7 30.453713826800563 35.0 19.0 37.0 17.0 37.0 8 33.65532879818594 35.0 32.0 37.0 28.0 37.0 9 36.58312074829932 37.0 35.0 39.0 32.0 39.0 10 36.794612487852284 38.0 35.0 39.0 33.0 39.0 11 36.9787347478674 39.0 37.0 39.0 33.0 39.0 12 36.86845035633301 39.0 35.0 39.0 33.0 39.0 13 36.63875728862974 39.0 35.0 39.0 33.0 39.0 14 37.6680569053018 39.0 36.0 41.0 33.0 41.0 15 37.4819572670338 39.0 36.0 41.0 32.0 41.0 16 37.89552626606198 40.0 36.0 41.0 33.0 41.0 17 37.83313762012742 40.0 36.0 41.0 33.0 41.0 18 37.916855631141345 40.0 36.0 41.0 33.0 41.0 19 37.94442419825073 40.0 36.0 41.0 34.0 41.0 20 37.71783217255156 39.0 36.0 41.0 33.0 41.0 21 37.73625620883274 39.0 36.0 41.0 33.0 41.0 22 37.828723949897416 39.0 36.0 41.0 33.0 41.0 23 37.66486475542598 39.0 36.0 41.0 33.0 41.0 24 37.65374284634489 39.0 36.0 41.0 33.0 41.0 25 37.47055865997193 39.0 35.0 41.0 33.0 41.0 26 37.36187371774106 39.0 35.0 40.0 33.0 41.0 27 36.47083873231833 39.0 35.0 40.0 31.0 41.0 28 36.85859720872476 39.0 35.0 40.0 31.0 41.0 29 37.11309523809524 39.0 35.0 40.0 31.0 41.0 30 37.10649497894396 39.0 35.0 40.0 32.0 41.0 31 37.215601716877224 39.0 35.0 40.0 32.0 41.0 32 37.033966364323504 39.0 35.0 40.0 32.0 41.0 33 35.85190921606738 39.0 35.0 40.0 30.0 41.0 34 36.3262336680704 39.0 35.0 40.0 30.0 41.0 35 36.62113972573156 39.0 35.0 40.0 30.0 41.0 36 36.727472735125794 39.0 35.0 40.0 31.0 41.0 37 36.85859383435914 39.0 35.0 40.0 31.0 41.0 38 36.77250364431487 39.0 35.0 40.0 31.0 41.0 39 36.75887458157867 39.0 35.0 40.0 31.0 41.0 40 36.66192230860598 39.0 35.0 40.0 31.0 41.0 41 36.53605172227621 39.0 35.0 40.0 31.0 41.0 42 36.73139037360976 39.0 35.0 40.0 31.0 41.0 43 36.611775861138106 39.0 35.0 40.0 31.0 41.0 44 36.717430623042866 39.0 35.0 40.0 32.0 41.0 45 36.6722613648634 39.0 35.0 40.0 32.0 41.0 46 36.56208157866321 39.0 35.0 40.0 31.0 41.0 47 36.37336343267466 38.0 35.0 40.0 31.0 41.0 48 36.41664642047295 38.0 35.0 40.0 31.0 41.0 49 36.50620208400821 38.0 35.0 40.0 31.0 41.0 50 36.40652332361516 38.0 35.0 40.0 31.0 41.0 51 35.72346736313573 38.0 34.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 4.0 12 6.0 13 2.0 14 5.0 15 4.0 16 17.0 17 20.0 18 35.0 19 89.0 20 193.0 21 379.0 22 745.0 23 1061.0 24 1434.0 25 1678.0 26 2066.0 27 2298.0 28 2814.0 29 3743.0 30 5535.0 31 7866.0 32 11308.0 33 17788.0 34 23752.0 35 30898.0 36 36134.0 37 51273.0 38 55158.0 39 40025.0 40 20.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.599422308605984 4.008071482561278 48.973517978620016 16.41898823021272 2 17.32163103336573 16.991955512363678 45.850880034553505 19.835533419717095 3 16.39671741712558 17.475502105604146 47.29443364647447 18.83334683079581 4 17.437034337544542 3.729011445848181 56.87054583738257 21.963408379224706 5 21.604713313896987 20.610962639023864 37.73114404491956 20.053180002159593 6 14.776009610193283 35.42206565165748 36.58790897311305 13.214015765036175 7 71.36850772054854 1.9082037576935538 20.638295000539898 6.084993521218011 8 69.96544649605873 10.798644854767304 10.948804124824534 8.287104524349424 9 65.23762282690853 4.49431756829716 12.681540870316379 17.58651873447792 10 43.547200626282255 19.91550588489364 19.571995464852606 16.965298023971492 11 33.10387647122341 20.23607061872368 25.647878198898606 21.0121747111543 12 25.601649389914694 17.043245869776484 31.65762336680704 25.69748137350178 13 23.19336464744628 22.989890400604686 36.59263308498002 17.22411186696901 14 18.20807688154627 30.513713421876687 29.099179354281397 22.17903034229565 15 13.265643559010906 21.63407029478458 43.963597343699384 21.13668880250513 16 14.491550588489362 21.884448223733937 32.90580120937264 30.71819997840406 17 15.88246409674981 22.989890400604686 32.067271352985635 29.06037414965986 18 16.42708670769895 21.72382842025699 33.91878576827557 27.93029910376849 19 19.473463988770114 24.117265954000647 29.361030126336246 27.048239930892993 20 22.22897095346075 25.16871828096318 33.387660619803476 19.214650145772595 21 20.913980671633734 29.863810603606524 29.14304610733182 20.079162617427922 22 18.547200626282258 22.39026563006155 30.72359896339488 28.33893478026131 23 18.696685023215636 29.06273620559335 27.4349422308606 24.80563654033042 24 20.94738689126444 22.90620613324695 28.02140697548861 28.125 25 16.723356009070294 30.208670769895257 26.196887485152793 26.871085735881657 26 16.215851419933053 26.77964042759961 28.979389374797538 28.0251187776698 27 19.629697116942015 29.724786740092863 25.244304070834684 25.40121207213044 28 15.163724219846669 25.369155598747433 32.26062250296945 27.206497678436453 29 20.39905247813411 23.620559334845048 28.38718820861678 27.59319997840406 30 18.80095292085088 28.638578447251916 26.103080120937268 26.45738851095994 31 22.19657704351582 24.519827772378793 22.2910592808552 30.99253590325019 32 25.193013713421873 29.746720116618075 20.6946469063816 24.36561926357845 33 19.642857142857142 29.21154572940287 20.949074074074073 30.196523053665913 34 20.15339866105172 25.94414750026995 26.702367454918473 27.200086383759853 35 17.819012525645178 25.035768275564195 23.559820753698304 33.58539844509232 36 18.86607817730267 31.211194795378468 22.55965878414858 27.363068243170286 37 19.086761688802508 23.307418205377388 28.86432350718065 28.741496598639454 38 19.61350016196955 27.556419393154087 20.404451463124932 32.42562898175143 39 23.906368102796673 26.567055393586003 27.776090594968146 21.750485908649175 40 19.625310441636973 25.367805852499732 28.549832631465282 26.457051074398013 41 16.910970737501348 26.707091566785447 27.469360760177086 28.912576935536123 42 19.03985800669474 22.053166504697117 27.744708994708994 31.16226649389915 43 21.52406597559659 26.774241442608787 24.192514307310226 27.5091782744844 44 20.82725947521866 22.152710290465393 28.30957779937372 28.71045243494223 45 19.033784148580065 21.560509124284636 26.18406489579959 33.22164183133571 46 26.568067703271787 24.056527372853903 25.657663859194475 23.71774106467984 47 15.880776913940178 23.51021757909513 35.16898823021272 25.44001727675197 48 19.201490119857468 23.804462261094912 27.74234693877551 29.25170068027211 49 20.08962315084764 18.781044163697224 34.67093186480942 26.458400820645718 50 21.516979807796137 20.830296404275998 29.054637728107114 28.598086059820755 51 18.905558255048053 20.513780909189073 25.02497030558255 35.55569053018033 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 62.0 1 166.5 2 271.0 3 467.0 4 663.0 5 478.0 6 293.0 7 344.5 8 396.0 9 447.5 10 499.0 11 618.5 12 738.0 13 704.5 14 671.0 15 698.5 16 726.0 17 670.0 18 614.0 19 598.0 20 582.0 21 689.0 22 796.0 23 858.5 24 921.0 25 980.0 26 1209.5 27 1380.0 28 1697.5 29 2015.0 30 2559.5 31 3104.0 32 3182.0 33 3260.0 34 3763.0 35 4266.0 36 4870.5 37 5475.0 38 6603.5 39 7732.0 40 9405.0 41 11078.0 42 14320.0 43 17562.0 44 21487.5 45 25413.0 46 40564.5 47 55716.0 48 48795.0 49 41874.0 50 37283.5 51 32693.0 52 26536.0 53 20379.0 54 17528.0 55 14677.0 56 12938.5 57 11200.0 58 9898.0 59 8596.0 60 7774.5 61 6953.0 62 5954.0 63 4955.0 64 4071.5 65 3188.0 66 2748.0 67 2308.0 68 1819.5 69 1331.0 70 1161.0 71 991.0 72 969.0 73 947.0 74 771.0 75 409.0 76 223.0 77 155.0 78 87.0 79 61.5 80 36.0 81 27.0 82 18.0 83 23.0 84 28.0 85 14.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 296352.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.13388869655841 #Duplication Level Percentage of deduplicated Percentage of total 1 69.36853442721929 25.065549020365218 2 12.919540926592237 9.3366650770423 3 5.059193510221022 5.484250055780312 4 2.8369488204173328 4.100399716591703 5 1.7661455683655962 3.1908853694621175 6 1.3099585879334723 2.840033868809335 7 1.0411670004235267 2.6334988755483306 8 0.823174404652792 2.379559385246378 9 0.6718768716741447 2.184977168897886 >10 4.033251112543849 24.375348248364332 >50 0.10053266529608051 2.4888952550677605 >100 0.05275476495734918 3.8905173372930593 >500 0.0059722375423414175 1.6041145719078342 >1k 0.008958356313512125 5.8866688785685035 >5k 0.0019907458474471393 4.538637171054924 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC 6720 2.2675736961451247 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG 5899 1.9905382788035848 No Hit GAATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC 4254 1.4354551344347262 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCGATCAT 2289 0.7723922902494331 No Hit CCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC 1669 0.5631816218550912 No Hit GAATGATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTC 1539 0.5193148688046647 No Hit GAATGACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCT 1522 0.5135784472519166 No Hit GCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC 1445 0.4875958319835871 No Hit GAATCAGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC 1429 0.4821968469927653 No Hit TCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC 1113 0.37556689342403626 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCGATCATCGTA 1107 0.37354227405247814 No Hit CTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT 995 0.3357493791167261 No Hit GAACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT 978 0.33001295756397797 No Hit GAATATGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC 804 0.27129899578879174 No Hit GAATAATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC 599 0.20212450059388837 No Hit CCTGTATCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC 562 0.18963934780261313 No Hit GCTGTATCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC 522 0.1761418853255588 No Hit GAATAATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG 490 0.16534391534391535 No Hit TCCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG 479 0.1616321131627254 No Hit TCTGTATCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC 395 0.13328744196091136 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACCGATCATCGT 391 0.1319376957132059 No Hit GAACTATCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT 377 0.1272135838462369 No Hit GAATGATACGGCCTGTCTCTTATACACATCTGACGCACCGATCATCGTATG 374 0.12620127416045784 No Hit CTGTCACTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT 335 0.11304124824532988 No Hit CGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG 315 0.10629251700680273 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.3607196846992765 0.0 2 0.0 0.0 0.0 1.9186642911132707 0.0 3 0.0 0.0 0.0 2.6600124176654787 0.0 4 0.0 0.0 0.0 3.677721088435374 0.0 5 0.0 0.0 0.0 6.319511931756829 0.0 6 0.0 0.0 0.0 7.313937479753807 0.0 7 0.0 0.0 0.0 9.02001673685347 0.0 8 0.0 0.0 0.0 11.470143613000756 0.0 9 0.0 0.0 0.0 12.271218011013929 0.0 10 0.0 0.0 0.0 16.064004967066193 0.0 11 0.0 0.0 0.0 18.76957132059173 0.0 12 0.0 0.0 0.0 24.09195821185617 0.0 13 0.0 0.0 0.0 25.153196199114568 0.0 14 3.3743656192635786E-4 0.0 0.0 25.51965230536659 0.0 15 3.3743656192635786E-4 0.0 0.0 26.332536983047188 0.0 16 3.3743656192635786E-4 0.0 0.0 27.399848828420257 0.0 17 3.3743656192635786E-4 0.0 0.0 28.79211208292841 0.0 18 3.3743656192635786E-4 0.0 0.0 30.37536443148688 0.0 19 3.3743656192635786E-4 0.0 0.0 32.276144584818056 0.0 20 3.3743656192635786E-4 0.0 0.0 33.31308713961775 0.0 21 3.3743656192635786E-4 0.0 0.0 34.51942284850448 0.0 22 3.3743656192635786E-4 0.0 0.0 35.7179975164669 0.0 23 3.3743656192635786E-4 0.0 0.0 36.798469387755105 0.0 24 3.3743656192635786E-4 0.0 0.0 37.69672551560306 0.0 25 3.3743656192635786E-4 0.0 0.0 38.4191771946874 0.0 26 3.3743656192635786E-4 0.0 0.0 39.05996922578555 0.0 27 3.3743656192635786E-4 0.0 0.0 39.72168232372314 0.0 28 3.3743656192635786E-4 0.0 0.0 40.36821077637404 0.0 29 3.3743656192635786E-4 0.0 0.0 41.01507666558687 0.0 30 3.3743656192635786E-4 0.0 0.0 41.774983803045025 0.0 31 3.3743656192635786E-4 0.0 0.0 42.467066191555986 0.0 32 3.3743656192635786E-4 0.0 0.0 43.10718334953029 0.0 33 3.3743656192635786E-4 0.0 0.0 43.71389428787388 0.0 34 3.3743656192635786E-4 0.0 0.0 44.3030585249973 0.0 35 3.3743656192635786E-4 0.0 0.0 44.959372637944064 0.0 36 3.3743656192635786E-4 0.0 0.0 45.55562304286794 0.0 37 3.3743656192635786E-4 0.0 0.0 46.19000377928949 0.0 38 3.3743656192635786E-4 0.0 0.0 46.74711154302991 0.0 39 3.3743656192635786E-4 0.0 0.0 47.400726163481266 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCTCGG 30 2.1600317E-6 45.000004 12 AGGGTAC 30 2.1600317E-6 45.000004 6 CGTTAGG 75 0.0 45.000004 2 CGAATGC 30 2.1600317E-6 45.000004 45 GTCGGGA 30 2.1600317E-6 45.000004 4 CGGGTAC 20 7.024806E-4 45.0 6 TAATAGG 20 7.024806E-4 45.0 18 TAATACG 180 0.0 45.0 4 ATTAGTA 20 7.024806E-4 45.0 15 GTCTGCA 20 7.024806E-4 45.0 10 CTCCGCA 20 7.024806E-4 45.0 28 GCGAATC 20 7.024806E-4 45.0 9 GCGAAGA 20 7.024806E-4 45.0 35 TTTCGTG 35 1.2078453E-7 45.0 1 CCGTGAC 35 1.2078453E-7 45.0 37 GCACGAT 20 7.024806E-4 45.0 41 CACGATG 20 7.024806E-4 45.0 42 CAATTGA 20 7.024806E-4 45.0 14 GGTCACG 20 7.024806E-4 45.0 9 TAAGTAT 20 7.024806E-4 45.0 23 >>END_MODULE