##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934152.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	639453
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.833787627863188	31.0	31.0	34.0	30.0	34.0
2	32.057467867067636	33.0	31.0	34.0	30.0	34.0
3	32.1195209030218	33.0	31.0	34.0	30.0	34.0
4	35.61475823868212	37.0	35.0	37.0	33.0	37.0
5	29.762866074598133	37.0	35.0	37.0	0.0	37.0
6	23.900058331104866	35.0	15.0	37.0	0.0	37.0
7	30.434904519956902	35.0	19.0	37.0	17.0	37.0
8	34.19481025188716	35.0	32.0	37.0	32.0	37.0
9	36.75645747224581	37.0	35.0	39.0	34.0	39.0
10	36.88491257371535	39.0	37.0	39.0	33.0	39.0
11	37.1119550615917	39.0	37.0	39.0	34.0	39.0
12	37.07837167078738	39.0	37.0	39.0	34.0	39.0
13	36.94115595673177	39.0	37.0	39.0	33.0	39.0
14	37.90806517445379	40.0	37.0	41.0	33.0	41.0
15	37.825852720997474	40.0	37.0	41.0	33.0	41.0
16	38.091714324586796	40.0	37.0	41.0	33.0	41.0
17	38.14292528145149	40.0	37.0	41.0	34.0	41.0
18	38.21650379308565	40.0	37.0	41.0	34.0	41.0
19	38.23308046095647	40.0	37.0	41.0	34.0	41.0
20	38.18430752533806	40.0	37.0	41.0	34.0	41.0
21	38.064462908141806	40.0	37.0	41.0	34.0	41.0
22	38.13449932989602	40.0	37.0	41.0	34.0	41.0
23	38.11551748134734	40.0	37.0	41.0	34.0	41.0
24	38.09052737261378	40.0	37.0	41.0	34.0	41.0
25	37.84801697701004	40.0	36.0	41.0	33.0	41.0
26	37.660525480371504	39.0	36.0	41.0	33.0	41.0
27	36.89834905771026	39.0	36.0	41.0	32.0	41.0
28	37.24257138523082	39.0	36.0	41.0	32.0	41.0
29	37.52447482457663	39.0	36.0	41.0	32.0	41.0
30	37.575671706912004	39.0	36.0	41.0	33.0	41.0
31	37.556829039819974	39.0	36.0	41.0	33.0	41.0
32	37.58443231949807	39.0	36.0	41.0	33.0	41.0
33	36.51802556247293	39.0	35.0	41.0	31.0	41.0
34	36.98787713874202	39.0	35.0	41.0	31.0	41.0
35	37.274744195429534	39.0	35.0	41.0	32.0	41.0
36	37.36413778651441	39.0	35.0	41.0	33.0	41.0
37	37.43766469154105	39.0	35.0	41.0	33.0	41.0
38	37.364713278380115	39.0	35.0	41.0	33.0	41.0
39	37.45612109099496	39.0	35.0	41.0	33.0	41.0
40	37.39897224659201	39.0	35.0	41.0	33.0	41.0
41	37.30892184413866	39.0	35.0	41.0	33.0	41.0
42	37.37269666418017	39.0	35.0	41.0	33.0	41.0
43	37.26987753595651	39.0	35.0	41.0	33.0	41.0
44	37.25390294517345	39.0	35.0	41.0	33.0	41.0
45	37.207666552506595	39.0	35.0	40.0	33.0	41.0
46	37.15973026946468	39.0	35.0	40.0	33.0	41.0
47	37.12010734174365	39.0	35.0	40.0	33.0	41.0
48	37.10788126727062	39.0	35.0	40.0	32.0	41.0
49	37.18209626039756	39.0	35.0	40.0	33.0	41.0
50	37.1054495013707	39.0	35.0	40.0	32.0	41.0
51	36.353977540178875	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	5.0
12	11.0
13	6.0
14	6.0
15	15.0
16	29.0
17	36.0
18	78.0
19	111.0
20	246.0
21	443.0
22	671.0
23	1031.0
24	1588.0
25	2276.0
26	3089.0
27	4045.0
28	5130.0
29	6982.0
30	10061.0
31	14237.0
32	21023.0
33	33849.0
34	46094.0
35	58875.0
36	77078.0
37	115175.0
38	130458.0
39	106754.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.238768134640075	4.732169526141875	53.20375383335445	18.825308505863607
2	21.17591128667783	4.481173753192182	51.54780726652311	22.79510769360688
3	20.860954597132235	4.666644772954384	51.8824682971227	22.58993233279068
4	21.628329212623914	4.235807791972201	49.269610119899355	24.866252875504532
5	15.008921687755002	21.635366477286055	41.06150100163733	22.294210833321603
6	16.34349983501524	29.38464593957648	39.33909137966356	14.932762845744724
7	78.00276173542075	2.2994653242693364	12.747926743638704	6.949846196671218
8	77.1728336562656	2.858067754784167	11.114186656407899	8.854911932542345
9	70.76688982614829	5.942422664370954	14.89851482438897	8.39217268509179
10	34.94549247560024	27.499910079395985	21.46913064760037	16.085466797403406
11	27.872885106489452	23.49664478859275	27.85927972814265	20.771190376775152
12	25.29896646039662	20.24214445784131	33.90569752585413	20.55319155590794
13	23.813165314729932	24.001764007675312	33.53178419680571	18.65328648078905
14	17.635228859665997	26.801031506615814	32.76190744276749	22.8018321909507
15	15.265703656093569	25.152122204446613	37.4867269369289	22.095447202530913
16	17.309325313979294	25.424073387723574	32.90812616408086	24.358475134216278
17	18.586667041987447	24.31015258353624	29.168836489937494	27.934343884538816
18	19.119466168741095	23.00012667076392	33.5560236639753	24.32438349651968
19	20.86908654740849	24.538785493226243	30.799136136666803	23.79299182269846
20	22.44496468075058	26.663101119237847	30.88702375311399	20.004910446897583
21	22.08387481175317	25.914492542845213	31.882718510977355	20.118914134424266
22	19.158562083530768	25.032332321531058	29.14068743128893	26.668418163649243
23	19.260055078324754	25.18027126309518	29.148819381565183	26.410854277014884
24	21.355439727392007	23.6649136058475	28.85372341673274	26.12592325002776
25	19.631779036144955	25.975482169917104	26.589444415774103	27.803294378163834
26	19.141359881023313	28.578800943931764	27.29817515908128	24.981664015963645
27	21.124304679155465	27.818932744079707	28.6271234946118	22.429639082153027
28	16.771834677450883	24.662484967620763	33.730860594914716	24.834819760013637
29	18.12064373769456	23.960947872634893	32.943781638369046	24.974626751301503
30	23.23814260000344	23.03093425161818	29.330537193507578	24.400385954870803
31	22.73990426192386	25.858038041888925	27.635807479204882	23.766250216982325
32	21.273963840970328	25.396080712734165	27.16477989781892	26.165175548476586
33	21.747337177243676	24.271056668746567	28.008626122639193	25.97298003137056
34	19.554681892179723	22.556153462412407	31.282205259807995	26.606959385599882
35	19.202193124436043	23.204207345966005	28.813845583647275	28.77975394595068
36	23.58375048674414	23.37857512592794	29.365879900477438	23.67179448685048
37	21.569841724098566	23.480537271699404	33.39197720551784	21.557643798684186
38	18.432316370397825	24.385060356273254	32.016426539558026	25.166196733770896
39	21.188578363069684	20.479847619762516	31.91727929965142	26.41429471751638
40	24.397571049005947	20.756803080132553	28.875929896333275	25.96969597452823
41	20.370691825669752	22.435112510223583	27.01934309480134	30.174852569305326
42	21.190298583320434	21.009988224310465	30.509357216245757	27.290355976123344
43	22.862509050704276	19.99224337050573	32.68856350662207	24.45668407216793
44	20.310953267871135	20.524104195304425	31.5479010967186	27.617041440105837
45	21.454430583639457	19.70621765790449	28.394893760761153	30.444457997694908
46	22.653424098409108	20.636856813557838	30.9475442292084	25.76217485882465
47	18.567275468251772	21.4678795783271	34.94330310437202	25.02154184904911
48	18.234647425221244	20.066994759583583	33.91179648856132	27.78656132663386
49	21.287569219317135	17.537958223669293	36.1823308358863	24.992141721127275
50	21.620666413325136	17.837745698276496	31.404653664929246	29.13693422346912
51	19.240976271907396	17.899986394621653	28.338282876145705	34.520754457325246
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	178.0
1	364.0
2	550.0
3	1068.0
4	1586.0
5	1184.5
6	783.0
7	855.5
8	928.0
9	1111.5
10	1295.0
11	1307.0
12	1319.0
13	1321.5
14	1324.0
15	1244.5
16	1165.0
17	1251.0
18	1337.0
19	1391.5
20	1446.0
21	1636.0
22	1826.0
23	2103.0
24	2380.0
25	2704.0
26	3838.5
27	4649.0
28	5377.0
29	6105.0
30	7078.5
31	8052.0
32	9021.5
33	9991.0
34	11519.5
35	13048.0
36	14541.5
37	16035.0
38	18157.5
39	20280.0
40	23902.5
41	27525.0
42	32315.5
43	37106.0
44	44379.5
45	51653.0
46	63242.0
47	74831.0
48	86889.5
49	98948.0
50	91253.0
51	83558.0
52	66080.0
53	48602.0
54	40076.0
55	31550.0
56	27379.0
57	23208.0
58	20998.5
59	18789.0
60	16889.5
61	14990.0
62	12644.0
63	10298.0
64	8553.0
65	6808.0
66	5687.0
67	4566.0
68	3993.0
69	3420.0
70	2933.0
71	2446.0
72	2166.5
73	1887.0
74	1435.5
75	726.0
76	468.0
77	395.0
78	322.0
79	216.5
80	111.0
81	73.5
82	36.0
83	36.0
84	36.0
85	20.5
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	639453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.89045921663929
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.48738133177498	27.718835510862323
2	13.0408338377195	10.404097007090387
3	5.215654436793179	6.241645517969462
4	2.9149987709356355	4.651225583542465
5	1.8701040842764602	3.729965535235035
6	1.2800294016660096	3.0636576385954286
7	0.9748317985305923	2.7220541671657403
8	0.7561398334672564	2.4130212151201613
9	0.5641893906754782	2.025519649128066
>10	3.7339990676984423	25.2415037138854
>50	0.09759078190381813	2.736250977489873
>100	0.0569279559381141	4.218650498498039
>500	0.003659654310307335	1.070396594878059
>1k	0.00325302605360652	2.7727472939605304
>5k	4.06628256700815E-4	0.990429096579092
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	6106	0.9548786228229441	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	4100	0.6411730025506175	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	3218	0.5032426151726553	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	2670	0.4175443699536948	No Hit
CCTGTATCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	2067	0.3232450234810064	No Hit
CTGTCACTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	1536	0.24020530046774355	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	1251	0.19563595760751767	No Hit
TCTGTATCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	1172	0.18328164853398138	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	1080	0.16889435189138216	No Hit
GCTGTATCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	985	0.1540379042713069	No Hit
CCTGACTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	961	0.1502846964514984	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	782	0.12229202146209338	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	723	0.11306538557173083	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	687	0.1074355738420181	No Hit
CTGTATCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	687	0.1074355738420181	No Hit
TGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	646	0.10102384381651193	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.680894451976924	0.0
2	0.0	0.0	0.0	2.7195118327695704	0.0
3	0.0	0.0	0.0	3.731470491185435	0.0
4	0.0	0.0	0.0	4.706678989699008	0.0
5	0.0	0.0	0.0	6.355588291868206	0.0
6	0.0	0.0	0.0	7.481081486833278	0.0
7	0.0	0.0	0.0	8.862261964522803	0.0
8	0.0	0.0	0.0	10.956239160657624	0.0
9	0.0	0.0	0.0	11.856696270093346	0.0
10	0.0	0.0	0.0	13.66558605558188	0.0
11	0.0	0.0	0.0	17.113532972712616	0.0
12	0.0	0.0	0.0	20.01476261742458	0.0
13	0.0	0.0	0.0	20.980118945411157	0.0
14	0.0	0.0	0.0	21.331825794859043	0.0
15	0.0	0.0	0.0	21.842262058352997	0.0
16	0.0	0.0	0.0	23.11647611317798	0.0
17	0.0	0.0	0.0	24.839511269788396	0.0
18	0.0	0.0	0.0	26.880318021809266	0.0
19	0.0	0.0	0.0	28.078998769260604	0.0
20	0.0	0.0	0.0	29.38136188273415	0.0
21	4.6915097747606156E-4	0.0	0.0	30.97147092905968	0.0
22	4.6915097747606156E-4	0.0	0.0	32.55579377999634	0.0
23	4.6915097747606156E-4	0.0	0.0	34.00593945137485	0.0
24	4.6915097747606156E-4	0.0	0.0	35.102501669395565	0.0
25	4.6915097747606156E-4	0.0	0.0	36.09100277893762	0.0
26	4.6915097747606156E-4	0.0	0.0	37.021485550931814	0.0
27	4.6915097747606156E-4	0.0	0.0	37.924601182573234	0.0
28	4.6915097747606156E-4	0.0	0.0	38.851330746747614	0.0
29	4.6915097747606156E-4	0.0	0.0	39.857659593433766	0.0
30	4.6915097747606156E-4	0.0	0.0	40.92794935671582	0.0
31	4.6915097747606156E-4	0.0	0.0	41.837320334723586	0.0
32	4.6915097747606156E-4	0.0	0.0	42.650828129667076	0.0
33	4.6915097747606156E-4	0.0	0.0	43.46527422656552	0.0
34	4.6915097747606156E-4	0.0	0.0	44.295827840357305	0.0
35	4.6915097747606156E-4	0.0	0.0	45.21395630327796	0.0
36	4.6915097747606156E-4	0.0	0.0	46.033875828246956	0.0
37	4.6915097747606156E-4	0.0	0.0	46.849572994418665	0.0
38	4.6915097747606156E-4	0.0	0.0	47.66229887106636	0.0
39	4.6915097747606156E-4	0.0	0.0	48.67863627193867	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTAG	30	2.1639844E-6	45.000004	1
AAACGGG	30	2.1639844E-6	45.000004	22
GTCCATA	65	0.0	45.000004	22
TGCGTAG	35	1.2107921E-7	45.000004	1
GCATAAC	20	7.030937E-4	45.0	30
CCGATTA	25	3.8888575E-5	45.0	12
CCGATAA	20	7.030937E-4	45.0	15
ACCGCTA	20	7.030937E-4	45.0	11
ACCGAAC	25	3.8888575E-5	45.0	11
CAACCCG	20	7.030937E-4	45.0	23
ACACAAT	20	7.030937E-4	45.0	10
CGACCAT	20	7.030937E-4	45.0	13
TCGGCGT	50	2.1827873E-11	45.0	4
CTAACCG	20	7.030937E-4	45.0	15
CACTCGC	25	3.8888575E-5	45.0	22
TGACCAC	40	6.8066583E-9	45.0	24
TCTACGT	20	7.030937E-4	45.0	41
ACGGCAA	20	7.030937E-4	45.0	27
TGATTCG	20	7.030937E-4	45.0	11
GCGGGTA	40	6.8066583E-9	45.0	5
>>END_MODULE