FastQCFastQC Report
Sat 14 Jan 2017
SRR2934150.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934150.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1882927
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC88480.46990669314317546TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT75920.40320203597909TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC57470.3052162935684708TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC48790.25911785215252636TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC30150.16012304247588993TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCACTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT28030.14886397613927677TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC25070.13314377031079802TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC22550.11976035183520126TruSeq Adapter, Index 16 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG22290.1183795229448619TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATCG207.0344337E-445.0000042
TCGTCAA207.0344337E-445.00000410
TATTCGA253.8917573E-545.015
GCGTTAG1950.043.8461531
CGTTAGG5100.042.7941172
TATGGGA22400.042.2879454
ACGGGTA1600.042.1875045
CTCGTAG1500.042.01
CCGTTAG650.041.5384641
CGTTTAT4750.041.2105261
TTATGGG46450.041.027993
TTAGGGA29600.040.9712834
TAGGGAC12650.040.553365
TAGGGCG7850.040.4140135
TACGGGT2450.040.4081654
CGGGTAC2250.040.06
TTACGGG17800.039.691013
TAGGGAG14250.039.631585
AGGGCGA8850.039.406786
GTATGGG18350.039.3596733
Produced by FastQC (version 0.11.5)