##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934150.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1882927
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.849970816712492	31.0	31.0	34.0	30.0	34.0
2	32.071653866559885	33.0	31.0	34.0	30.0	34.0
3	32.15300115192995	33.0	31.0	34.0	30.0	34.0
4	35.61437432253083	37.0	35.0	37.0	33.0	37.0
5	29.168041033985915	37.0	33.0	37.0	0.0	37.0
6	23.385105211195125	35.0	10.0	37.0	0.0	37.0
7	30.23153951268424	35.0	19.0	37.0	17.0	37.0
8	34.175890515139464	35.0	32.0	37.0	32.0	37.0
9	36.78559179405256	37.0	37.0	39.0	34.0	39.0
10	36.882557847436466	39.0	37.0	39.0	33.0	39.0
11	37.036434232447675	39.0	37.0	39.0	34.0	39.0
12	36.94503557493201	39.0	35.0	39.0	33.0	39.0
13	36.80225415005467	39.0	35.0	39.0	33.0	39.0
14	37.53697514561106	40.0	37.0	41.0	32.0	41.0
15	37.510133956335004	40.0	36.0	41.0	32.0	41.0
16	37.847033899880344	40.0	36.0	41.0	33.0	41.0
17	37.937232298437486	40.0	36.0	41.0	33.0	41.0
18	38.00868063392792	40.0	37.0	41.0	33.0	41.0
19	38.03654416767087	40.0	37.0	41.0	33.0	41.0
20	37.94226170212653	40.0	37.0	41.0	33.0	41.0
21	37.83238808514616	40.0	36.0	41.0	33.0	41.0
22	37.9816519705756	40.0	36.0	41.0	34.0	41.0
23	37.96450420010972	40.0	36.0	41.0	34.0	41.0
24	37.93312592575283	40.0	36.0	41.0	34.0	41.0
25	37.695560688226365	39.0	36.0	41.0	33.0	41.0
26	37.60951539810094	39.0	36.0	41.0	33.0	41.0
27	36.73703760156395	39.0	35.0	41.0	32.0	41.0
28	37.130606762768814	39.0	35.0	41.0	32.0	41.0
29	37.41065213893051	39.0	35.0	41.0	32.0	41.0
30	37.432785763866576	39.0	35.0	41.0	33.0	41.0
31	37.40974928927144	39.0	35.0	41.0	33.0	41.0
32	37.47030925787351	39.0	35.0	41.0	33.0	41.0
33	36.287270828874405	39.0	35.0	41.0	31.0	41.0
34	36.762574969714706	39.0	35.0	41.0	31.0	41.0
35	37.09510777635033	39.0	35.0	41.0	31.0	41.0
36	37.25134803420419	39.0	35.0	41.0	32.0	41.0
37	37.31288148717396	39.0	35.0	41.0	33.0	41.0
38	37.23974110520482	39.0	35.0	41.0	33.0	41.0
39	37.25618571511269	39.0	35.0	41.0	33.0	41.0
40	37.19564964547218	39.0	35.0	41.0	33.0	41.0
41	37.1026901202224	39.0	35.0	40.0	32.0	41.0
42	37.19216570796425	39.0	35.0	41.0	33.0	41.0
43	37.09190159788457	39.0	35.0	40.0	32.0	41.0
44	37.127801024681254	39.0	35.0	40.0	33.0	41.0
45	37.0795702648058	39.0	35.0	40.0	33.0	41.0
46	37.060096859835774	39.0	35.0	40.0	33.0	41.0
47	37.01566125505662	39.0	35.0	40.0	33.0	41.0
48	36.96238037905877	39.0	35.0	40.0	32.0	41.0
49	37.02759958298967	39.0	35.0	40.0	33.0	41.0
50	36.94688057476471	39.0	35.0	40.0	32.0	41.0
51	36.239246662244476	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	19.0
10	23.0
11	20.0
12	20.0
13	20.0
14	35.0
15	56.0
16	90.0
17	147.0
18	281.0
19	478.0
20	766.0
21	1302.0
22	2122.0
23	3274.0
24	5154.0
25	7504.0
26	10106.0
27	13273.0
28	17290.0
29	23489.0
30	32618.0
31	47421.0
32	69387.0
33	111720.0
34	146921.0
35	165670.0
36	224257.0
37	334197.0
38	366199.0
39	298925.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.841710273420052	4.4082431236048985	54.2446945633049	17.505352039670154
2	20.369881572679134	4.102495742001682	52.87082292622072	22.65679975909847
3	22.01885681176169	4.024532018500983	51.2612544193163	22.69535675042102
4	20.557302540140963	4.57452678728384	49.1297857006671	25.738384971908097
5	14.872429998613859	23.127503084293764	40.598440619312385	21.40162629778
6	16.65295574390298	29.488344476445448	39.50115963072387	14.357540148927706
7	81.1683618111589	2.2088482453116876	10.787088400134472	5.835701543394937
8	81.19103927024256	2.553205727040931	9.388627387041558	6.867127615674957
9	75.34322891965542	5.136842798472803	12.511584357757895	7.008343924113893
10	41.34222941197402	22.587864532188448	19.540268953602556	16.52963710223498
11	29.915339256381156	22.24541896738429	26.375956157620557	21.463285618613998
12	27.456242329097197	19.07243350379489	31.880418093744474	21.59090607336344
13	26.554879716526454	20.30514194124361	33.664289693652485	19.475688648577457
14	20.274232617621397	22.01216510252389	36.36540343837015	21.34819884148456
15	17.949394745521204	22.97624921199813	37.056136536360675	22.018219506119994
16	22.98368444448457	22.801043269335455	30.6549324535683	23.560339832611675
17	23.718763393376378	22.435495375019848	28.49855570608951	25.347185525514266
18	23.266435714183288	21.830957865068587	31.861245815690147	23.041360605057974
19	24.016650672065353	23.720356657480615	29.62186000838057	22.641132662073463
20	22.77321425631477	27.96927337066174	29.63657114694303	19.62094122608046
21	22.566621010798613	24.770742572601062	32.72776905318156	19.93486736341876
22	20.694748123533202	22.56380624421446	32.451762601524116	24.289683030728224
23	20.333714477512935	23.07184505825239	31.547001025530996	25.047439438703677
24	22.36756921537585	24.461065139540725	28.400888616499735	24.77047702858369
25	22.672413747319997	24.170931746159038	25.847948433476176	27.308706073044785
26	19.674315573572425	23.621680500624826	28.008202123608616	28.695801802194136
27	19.593643301094517	24.966767166225775	31.411732903081212	24.027856629598492
28	19.12102805897414	22.465979828214262	29.92994417733667	28.483047935474925
29	20.09106035443753	24.082186935553	28.80010749221823	27.026645217791234
30	23.10248883785723	22.266768706381075	28.98922794139125	25.641514514370446
31	21.670941040199647	24.481087158450645	28.18967490508129	25.658296896268418
32	22.545855468640045	24.65746149478976	27.10375920043634	25.692923836133847
33	22.46247464718494	23.732837226297143	28.228709875635115	25.5759782508828
34	19.532143306670942	21.525900897910542	30.770815862749856	28.17113993266866
35	18.525572154417034	23.609518584629143	29.03006861126321	28.834840649690612
36	22.434539416557307	21.738601655826276	29.583993431503185	26.242865496113232
37	21.05036467159906	25.04749254750715	30.27493896470761	23.62720381618618
38	22.32433864934753	25.257378538838733	28.005334248220986	24.412948563592746
39	20.119314237886016	22.43746040074841	29.07855694883551	28.364668412530065
40	21.343259722761424	20.037048701303874	30.148593121241557	28.471098454693145
41	19.02314853417047	20.577324559050883	30.285985595830322	30.113541310948328
42	19.605061693841556	20.001306476565475	34.296549999017486	26.09708183057548
43	21.15674160495866	20.067692480908715	33.28551770727171	25.490048206860916
44	21.092426843950935	20.24146448587757	29.93711386580574	28.728994804365755
45	20.53552793071638	19.35465368545886	27.560335583907396	32.549482799917364
46	20.87117556867579	19.410895908338453	31.504620200358275	28.213308322627483
47	17.109372800963605	21.360413866283718	34.905176886836294	26.625036445916383
48	17.79978724613328	22.95558988744651	32.51459031603456	26.73003255038565
49	21.49892162574545	19.314715865245972	33.29528972711103	25.891072781897545
50	19.920687312891047	19.697099250263022	29.92558925545175	30.456624181394183
51	18.10834939432065	19.499906262961865	28.35903887936176	34.032705463355725
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	420.0
1	1142.5
2	1865.0
3	3561.5
4	5258.0
5	3787.5
6	2317.0
7	2328.0
8	2339.0
9	2553.0
10	2767.0
11	2837.0
12	2907.0
13	2847.0
14	2787.0
15	2909.0
16	3031.0
17	2911.5
18	2792.0
19	3244.5
20	3697.0
21	4118.5
22	4540.0
23	5311.5
24	6083.0
25	7552.5
26	10733.0
27	12444.0
28	13995.0
29	15546.0
30	18831.0
31	22116.0
32	26115.5
33	30115.0
34	34150.0
35	38185.0
36	41006.5
37	43828.0
38	49180.5
39	54533.0
40	62245.5
41	69958.0
42	81590.5
43	93223.0
44	109762.0
45	126301.0
46	157284.0
47	188267.0
48	221675.0
49	255083.0
50	245175.5
51	235268.0
52	200268.0
53	165268.0
54	143835.5
55	122403.0
56	109843.5
57	97284.0
58	86563.0
59	75842.0
60	64866.0
61	53890.0
62	47381.5
63	40873.0
64	35824.5
65	30776.0
66	26272.5
67	21769.0
68	18532.5
69	15296.0
70	12704.0
71	10112.0
72	8619.5
73	7127.0
74	5609.0
75	2921.5
76	1752.0
77	1333.5
78	915.0
79	698.5
80	482.0
81	349.0
82	216.0
83	150.0
84	84.0
85	61.0
86	38.0
87	26.5
88	15.0
89	8.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1882927.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.03774729362539
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.76719210326918	25.46981883213075
2	13.47658079233735	9.98284387537433
3	5.373916837694391	5.9711332143444995
4	2.719436827359886	4.028872559709352
5	1.6355164301411762	3.02879221170706
6	1.0883817148927204	2.4186724149119527
7	0.8148584115270416	2.1126363948356497
8	0.6616459101058734	1.9604699213089691
9	0.5497700466818747	1.8326019652747116
>10	4.725316466530641	31.088281532142346
>50	0.11290517641929868	2.9317666132686897
>100	0.06646897679929492	4.6514515430795464
>500	0.004942529382972494	1.207187031175748
>1k	0.0025564807153306	1.9149354621286074
>5k	5.112961430661201E-4	1.4005364286078392
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	8848	0.46990669314317546	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	7592	0.40320203597909	TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	5747	0.3052162935684708	TruSeq Adapter, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	4879	0.25911785215252636	TruSeq Adapter, Index 13 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	3015	0.16012304247588993	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCACTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGCT	2803	0.14886397613927677	TruSeq Adapter, Index 16 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2507	0.13314377031079802	TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTGC	2255	0.11976035183520126	TruSeq Adapter, Index 16 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCCTGACCTTTCGTATGCCGTCTTCTG	2229	0.1183795229448619	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5932641042377109E-4	0.0	0.0	0.4336864891735049	0.0
2	1.5932641042377109E-4	0.0	0.0	1.5584247291583795	0.0
3	1.5932641042377109E-4	0.0	0.0	2.160306798935912	0.0
4	1.5932641042377109E-4	0.0	0.0	2.809349486198881	0.0
5	1.5932641042377109E-4	0.0	0.0	3.9036032729893404	0.0
6	1.5932641042377109E-4	0.0	0.0	4.761310449103974	0.0
7	1.5932641042377109E-4	0.0	0.0	5.89114713422241	0.0
8	1.5932641042377109E-4	0.0	0.0	7.606508377648204	0.0
9	2.1243521389836145E-4	0.0	0.0	8.359697428524845	0.0
10	2.1243521389836145E-4	0.0	0.0	9.832404548875235	0.0
11	2.1243521389836145E-4	0.0	0.0	12.794282518653139	0.0
12	2.1243521389836145E-4	0.0	0.0	15.351152753133817	0.0
13	2.655440173729518E-4	0.0	0.0	16.170727808353696	0.0
14	2.655440173729518E-4	0.0	0.0	16.4848663809059	0.0
15	2.655440173729518E-4	0.0	0.0	16.917915564437706	0.0
16	2.655440173729518E-4	0.0	0.0	17.943499668335523	0.0
17	2.655440173729518E-4	0.0	0.0	19.25746457510036	0.0
18	2.655440173729518E-4	0.0	0.0	20.67796574163523	0.0
19	2.655440173729518E-4	0.0	0.0	21.71470269426271	0.0
20	2.655440173729518E-4	0.0	0.0	22.833067877830633	0.0
21	2.655440173729518E-4	0.0	0.0	24.363344941147478	0.0
22	2.655440173729518E-4	0.0	0.0	25.87168806862932	0.0
23	2.655440173729518E-4	0.0	0.0	27.220970329704762	0.0
24	2.655440173729518E-4	0.0	0.0	28.268116607813262	0.0
25	2.655440173729518E-4	0.0	0.0	29.178348390564267	0.0
26	2.655440173729518E-4	0.0	0.0	30.020707122474743	0.0
27	2.655440173729518E-4	0.0	0.0	30.93747128805312	0.0
28	2.655440173729518E-4	0.0	0.0	31.81950229615912	0.0
29	2.655440173729518E-4	0.0	0.0	32.82761360371379	0.0
30	2.655440173729518E-4	0.0	0.0	33.90731557835221	0.0
31	2.655440173729518E-4	0.0	0.0	34.79359529073618	0.0
32	2.655440173729518E-4	0.0	0.0	35.635582261022336	0.0
33	2.655440173729518E-4	0.0	0.0	36.437153431864324	0.0
34	2.655440173729518E-4	0.0	0.0	37.366132622241864	0.0
35	2.655440173729518E-4	0.0	0.0	38.34269730053263	0.0
36	2.655440173729518E-4	0.0	0.0	39.216177791279215	0.0
37	2.655440173729518E-4	0.0	0.0	40.09820879938521	0.0
38	2.655440173729518E-4	0.0	0.0	41.13579549286828	0.0
39	2.655440173729518E-4	0.0	0.0	42.99885231875692	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATCG	20	7.0344337E-4	45.000004	2
TCGTCAA	20	7.0344337E-4	45.000004	10
TATTCGA	25	3.8917573E-5	45.0	15
GCGTTAG	195	0.0	43.846153	1
CGTTAGG	510	0.0	42.794117	2
TATGGGA	2240	0.0	42.287945	4
ACGGGTA	160	0.0	42.187504	5
CTCGTAG	150	0.0	42.0	1
CCGTTAG	65	0.0	41.538464	1
CGTTTAT	475	0.0	41.210526	1
TTATGGG	4645	0.0	41.02799	3
TTAGGGA	2960	0.0	40.971283	4
TAGGGAC	1265	0.0	40.55336	5
TAGGGCG	785	0.0	40.414013	5
TACGGGT	245	0.0	40.408165	4
CGGGTAC	225	0.0	40.0	6
TTACGGG	1780	0.0	39.69101	3
TAGGGAG	1425	0.0	39.63158	5
AGGGCGA	885	0.0	39.40678	6
GTATGGG	1835	0.0	39.359673	3
>>END_MODULE