SRR2934149.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934149.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1272990
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	4381	0.3441503860988696	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	3084	0.24226427544599724	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	2548	0.20015868152931288	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2498	0.1962309209027565	Illumina PCR Primer Index 7 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2491	0.1956810344150386	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	2167	0.1702291455549533	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	1763	0.13849283969237777	No Hit
CCTGTATCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1542	0.12113213772299862	Illumina PCR Primer Index 7 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	335	0.0	42.313435	2
TATGGGT	725	0.0	40.96552	4
TTAGGGA	2990	0.0	40.7107	4
TTATGGG	3805	0.0	40.505913	3
TAGGGAT	1570	0.0	40.414013	5
CGAACGA	45	1.9286745E-8	40.000004	22
CAAATCG	45	1.9286745E-8	40.000004	31
CGTTATT	210	0.0	39.642857	1
CTAGGGA	1540	0.0	39.44805	4
TAGGGAG	1510	0.0	39.33775	5
CGTTCAT	115	0.0	39.130436	17
ACGGGAG	460	0.0	39.130436	5
TTGCGAG	115	0.0	39.130436	1
TGGGCGA	1115	0.0	38.744396	6
TATGGGA	2405	0.0	38.638252	4
TTTGTCG	140	0.0	38.571426	1
ACGTAGG	35	6.248818E-6	38.571426	2
TAGGGAC	1110	0.0	38.51351	5
ACGGGTA	135	0.0	38.333332	5
TTTATGG	2345	0.0	38.187634	2