##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934149.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1272990
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.016184730437786	33.0	31.0	34.0	30.0	34.0
2	32.22022403946614	33.0	31.0	34.0	30.0	34.0
3	32.30473138045075	34.0	31.0	34.0	30.0	34.0
4	35.72931444866024	37.0	35.0	37.0	35.0	37.0
5	29.59319633304268	37.0	35.0	37.0	0.0	37.0
6	23.674298305564065	35.0	10.0	37.0	0.0	37.0
7	30.411396790234015	35.0	19.0	37.0	17.0	37.0
8	34.323763737342794	35.0	32.0	37.0	32.0	37.0
9	37.00267637609094	38.0	37.0	39.0	34.0	39.0
10	37.00528362359484	39.0	37.0	39.0	34.0	39.0
11	37.19258046017644	39.0	37.0	39.0	34.0	39.0
12	37.22065530758294	39.0	37.0	39.0	34.0	39.0
13	37.19805968625048	39.0	37.0	39.0	34.0	39.0
14	38.347390788615776	40.0	38.0	41.0	34.0	41.0
15	38.21358141069451	40.0	38.0	41.0	33.0	41.0
16	38.431866707515375	40.0	38.0	41.0	34.0	41.0
17	38.420488770532366	40.0	38.0	41.0	34.0	41.0
18	38.47392516830454	40.0	38.0	41.0	34.0	41.0
19	38.495840501496474	40.0	38.0	41.0	34.0	41.0
20	38.45685904838216	40.0	38.0	41.0	34.0	41.0
21	38.35018342642126	40.0	37.0	41.0	34.0	41.0
22	38.412484779927574	40.0	38.0	41.0	34.0	41.0
23	38.363273081485325	40.0	37.0	41.0	34.0	41.0
24	38.345000353498456	40.0	37.0	41.0	34.0	41.0
25	38.11964901531041	40.0	37.0	41.0	34.0	41.0
26	37.99325760610845	40.0	37.0	41.0	34.0	41.0
27	37.13201360576281	40.0	36.0	41.0	33.0	41.0
28	37.50112255398707	40.0	36.0	41.0	33.0	41.0
29	37.73969866220473	40.0	36.0	41.0	33.0	41.0
30	37.77074211109278	40.0	36.0	41.0	33.0	41.0
31	37.80038963385415	40.0	37.0	41.0	33.0	41.0
32	37.76809401487836	40.0	36.0	41.0	33.0	41.0
33	36.58751364896818	40.0	36.0	41.0	31.0	41.0
34	37.08138948459925	40.0	36.0	41.0	31.0	41.0
35	37.356372791616586	40.0	36.0	41.0	31.0	41.0
36	37.49427646721498	40.0	36.0	41.0	33.0	41.0
37	37.56968554348424	40.0	36.0	41.0	33.0	41.0
38	37.474531614545285	40.0	36.0	41.0	33.0	41.0
39	37.45093205759668	40.0	36.0	41.0	33.0	41.0
40	37.4613233411103	40.0	36.0	41.0	33.0	41.0
41	37.40096230135351	40.0	36.0	41.0	33.0	41.0
42	37.471890588299985	40.0	36.0	41.0	33.0	41.0
43	37.335494387230064	39.0	35.0	41.0	33.0	41.0
44	37.3279381613367	39.0	35.0	41.0	33.0	41.0
45	37.21203073079914	39.0	35.0	41.0	32.0	41.0
46	37.118869747602105	39.0	35.0	41.0	32.0	41.0
47	37.03091618944375	39.0	35.0	40.0	32.0	41.0
48	36.90209428196608	39.0	35.0	40.0	32.0	41.0
49	36.85176081508889	39.0	35.0	40.0	32.0	41.0
50	36.71587129513979	39.0	35.0	40.0	32.0	41.0
51	35.94139938255603	37.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	7.0
11	9.0
12	12.0
13	13.0
14	21.0
15	29.0
16	44.0
17	86.0
18	125.0
19	265.0
20	456.0
21	809.0
22	1460.0
23	2220.0
24	3228.0
25	4636.0
26	6060.0
27	8000.0
28	9978.0
29	13445.0
30	18657.0
31	26450.0
32	38221.0
33	61071.0
34	85225.0
35	113033.0
36	151536.0
37	228178.0
38	267816.0
39	231753.0
40	142.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.490365203183057	4.2062388549792225	54.58432509289154	17.71907084894618
2	18.925914579061896	5.248980746117408	54.04009458047588	21.785010094344813
3	20.532761451386104	5.150393954390844	52.519894107573506	21.796950486649543
4	18.866683948813424	4.38235964147401	52.617852457599824	24.13310395211274
5	15.549690099686565	22.11376365878758	41.14737743422965	21.18916880729621
6	15.96045530601183	30.28287732032459	40.02953675991171	13.727130613751875
7	83.55996512148563	1.7829676588190009	9.840611473774343	4.81645574592102
8	85.41732456657162	2.745504677962906	6.900054203096646	4.937116552368832
9	80.79309342571428	4.256042859723957	9.020966386224558	5.929897328337222
10	37.3350144148815	28.505565636807827	17.397858584906402	16.76156136340427
11	27.592204180708414	26.558574694223836	25.371919653728625	20.47730147133913
12	24.589038405643407	22.415572785332174	31.262303710162687	21.733085098861736
13	23.484866338305878	22.541261125381975	32.87464944736408	21.099223088948065
14	19.720264888176654	25.370976991178253	32.19396853078186	22.714789589863233
15	17.763297433601206	25.114886998326774	34.792496406099026	22.329319161972993
16	20.617522525707194	24.497600138257177	31.654372775905543	23.23050456013009
17	20.989560012254614	24.61378329759071	29.730084289743047	24.666572400411628
18	21.315799809896387	23.987305477654967	31.435046622518637	23.261848089930005
19	22.86632259483578	24.906794240331816	29.496853863738124	22.730029301094273
20	24.443868372885884	26.377662039764648	29.350662613217697	19.827806974131768
21	24.079057965891327	25.920549258046016	29.463625008837464	20.536767767225193
22	22.530027729990024	23.66562188234	29.478550499218375	24.3257998884516
23	21.90472823824225	25.00443836950801	29.148225830524982	23.942607561724756
24	22.3752739613037	24.018727562667422	29.70816738544686	23.897831090582017
25	21.828529682087055	25.59666611678018	27.92362862237724	24.651175578755527
26	22.4560287197857	26.143017619934174	27.717813965545684	23.683139694734447
27	22.44754475683234	26.700681073692646	28.092050998043977	22.75972317143104
28	21.05617483248101	24.6937525039474	30.569211070000552	23.68086159357104
29	22.13348101713289	23.01204251408102	30.243599714059027	24.61087675472706
30	23.474968381526956	24.182515180794823	29.126151815803738	23.216364621874487
31	23.659494575762572	26.441684537977515	26.94852276922835	22.950298117031558
32	23.335454324071677	26.223693823203636	28.06196435164455	22.378887501080133
33	22.612275037510113	27.593225398471315	26.01599384127134	23.778505722747234
34	22.25382760273058	25.281502604105295	28.332822724451884	24.131847068712243
35	22.357834704121792	24.632165217322996	28.628033213143862	24.381966865411353
36	25.301769848938328	25.67679243356193	26.541292547466988	22.48014517003276
37	23.08855529108634	26.311282885175846	28.86377740594977	21.73638441778804
38	22.022168280976285	26.146238383647948	28.387497152373548	23.444096183002223
39	21.411558613971827	25.24536720634098	28.963856746714427	24.379217432972766
40	22.302139058437223	24.158477285760295	29.387583563107327	24.15180009269515
41	20.590106756533828	26.190386413090437	28.236749699526314	24.982757130849418
42	22.39656242389964	25.111901900250594	28.746965804915988	23.744569870933784
43	23.360513436869105	24.361071178878074	28.620806133590992	23.657609250661828
44	22.985019520970315	22.966244825175373	28.219624663194526	25.829110990659785
45	23.17638001869614	22.230261038971243	27.684506555432485	26.908852386900133
46	24.080236294079292	23.049749016095962	29.102113920769213	23.76790076905553
47	20.993959104156357	24.602707012623824	31.007392045499177	23.395941837720642
48	21.41077306184652	23.966252680696627	30.340379735897375	24.282594521559478
49	22.410231030880055	22.219184754004353	31.46049851137872	23.910085703736872
50	22.017297857799353	22.47684585110645	29.637781915018973	25.868074376075224
51	20.959709031492785	22.592164903102148	27.907524803808357	28.540601261596716
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	291.0
1	696.5
2	1102.0
3	2162.5
4	3223.0
5	2360.0
6	1497.0
7	1540.0
8	1583.0
9	1714.5
10	1846.0
11	1852.5
12	1859.0
13	1808.0
14	1757.0
15	1709.0
16	1661.0
17	1854.5
18	2048.0
19	2381.5
20	2715.0
21	3170.5
22	3626.0
23	4529.0
24	5432.0
25	6690.5
26	10165.5
27	12382.0
28	14386.5
29	16391.0
30	19276.0
31	22161.0
32	25736.0
33	29311.0
34	32539.5
35	35768.0
36	38609.0
37	41450.0
38	45275.0
39	49100.0
40	54677.0
41	60254.0
42	67102.5
43	73951.0
44	82790.5
45	91630.0
46	108215.0
47	124800.0
48	133196.5
49	141593.0
50	135136.5
51	128680.0
52	111876.0
53	95072.0
54	82864.0
55	70656.0
56	64821.0
57	58986.0
58	54425.0
59	49864.0
60	45349.0
61	40834.0
62	35454.0
63	30074.0
64	26582.0
65	23090.0
66	19107.5
67	15125.0
68	12619.5
69	10114.0
70	8158.5
71	6203.0
72	5151.5
73	4100.0
74	3134.0
75	1749.5
76	1331.0
77	1044.5
78	758.0
79	558.5
80	359.0
81	257.0
82	155.0
83	94.5
84	34.0
85	20.0
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1272990.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.88059614505861
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.39516267212402	25.25820402404187
2	11.451844628917218	8.217980244922748
3	4.403518717070325	4.740026301132208
4	2.3550056373770216	3.37996024776245
5	1.578871130228583	2.832541869441201
6	1.2394419411797137	2.668314944203008
7	1.0174527370163104	2.5554767527496733
8	0.8475710212075278	2.4329082812961778
9	0.7339185038101149	2.370009009473675
>10	5.844962254128738	38.05574523531333
>50	0.08497381601733268	2.068991525663669
>100	0.040723701995074336	2.4861235418611023
>500	0.004446840701215192	1.1126882436908834
>1k	0.0021063982268914065	1.8210297784480605
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	4381	0.3441503860988696	Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGCT	3084	0.24226427544599724	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGC	2548	0.20015868152931288	No Hit
GCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2498	0.1962309209027565	Illumina PCR Primer Index 7 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	2491	0.1956810344150386	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCG	2167	0.1702291455549533	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTC	1763	0.13849283969237777	No Hit
CCTGTATCTTATACACATCTGACGCGGGTCATATCGTATGCCGTCTTCTGC	1542	0.12113213772299862	Illumina PCR Primer Index 7 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26166741294118573	0.0
2	0.0	0.0	0.0	1.0722000958373592	0.0
3	0.0	0.0	0.0	1.4665472627436194	0.0
4	0.0	0.0	0.0	1.9220103849990966	0.0
5	0.0	0.0	0.0	2.767028806196435	0.0
6	0.0	0.0	0.0	3.3293270174942458	0.0
7	0.0	0.0	0.0	4.066174911036222	0.0
8	0.0	0.0	0.0	5.18079482164039	0.0
9	0.0	0.0	0.0	5.612534269711467	0.0
10	0.0	0.0	0.0	6.76446790626792	0.0
11	0.0	0.0	0.0	8.663225948357804	0.0
12	0.0	0.0	0.0	10.494740728521041	0.0
13	0.0	0.0	0.0	11.03260826872167	0.0
14	0.0	0.0	0.0	11.220041005820942	0.0
15	0.0	0.0	0.0	11.531198202656737	0.0
16	0.0	0.0	0.0	12.265610884610249	0.0
17	0.0	0.0	0.0	13.20693799637075	0.0
18	2.356656375933825E-4	0.0	0.0	14.253607648135493	0.0
19	2.356656375933825E-4	0.0	0.0	15.071917297072247	0.0
20	2.356656375933825E-4	0.0	0.0	15.838930392226176	0.0
21	2.356656375933825E-4	0.0	0.0	16.952921861130097	0.0
22	2.356656375933825E-4	0.0	0.0	18.15026041052954	0.0
23	2.356656375933825E-4	0.0	0.0	19.2355792268596	0.0
24	2.356656375933825E-4	0.0	0.0	20.077848215618346	0.0
25	2.356656375933825E-4	0.0	0.0	20.837084344731693	0.0
26	2.356656375933825E-4	0.0	0.0	21.505981979434246	0.0
27	2.356656375933825E-4	0.0	0.0	22.15830446429273	0.0
28	2.356656375933825E-4	0.0	0.0	22.842363255013787	0.0
29	2.356656375933825E-4	0.0	0.0	23.549438723006464	0.0
30	2.356656375933825E-4	0.0	0.0	24.3376617255438	0.0
31	2.356656375933825E-4	0.0	0.0	25.054163819040213	0.0
32	2.356656375933825E-4	0.0	0.0	25.736337284660525	0.0
33	2.356656375933825E-4	0.0	0.0	26.419060636768553	0.0
34	2.356656375933825E-4	0.0	0.0	27.108068405879074	0.0
35	2.356656375933825E-4	0.0	0.0	27.86384810564105	0.0
36	2.356656375933825E-4	0.0	0.0	28.551127660075885	0.0
37	2.356656375933825E-4	0.0	0.0	29.257810352005908	0.0
38	2.356656375933825E-4	0.0	0.0	29.964021712660745	0.0
39	2.356656375933825E-4	0.0	0.0	30.755544034124384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAGG	335	0.0	42.313435	2
TATGGGT	725	0.0	40.96552	4
TTAGGGA	2990	0.0	40.7107	4
TTATGGG	3805	0.0	40.505913	3
TAGGGAT	1570	0.0	40.414013	5
CGAACGA	45	1.9286745E-8	40.000004	22
CAAATCG	45	1.9286745E-8	40.000004	31
CGTTATT	210	0.0	39.642857	1
CTAGGGA	1540	0.0	39.44805	4
TAGGGAG	1510	0.0	39.33775	5
CGTTCAT	115	0.0	39.130436	17
ACGGGAG	460	0.0	39.130436	5
TTGCGAG	115	0.0	39.130436	1
TGGGCGA	1115	0.0	38.744396	6
TATGGGA	2405	0.0	38.638252	4
TTTGTCG	140	0.0	38.571426	1
ACGTAGG	35	6.248818E-6	38.571426	2
TAGGGAC	1110	0.0	38.51351	5
ACGGGTA	135	0.0	38.333332	5
TTTATGG	2345	0.0	38.187634	2
>>END_MODULE