FastQCFastQC Report
Sat 14 Jan 2017
SRR2934146.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934146.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1579131
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC64110.4059827842021973TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT53710.34012377693807544TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC41390.26210618371750033TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC35470.22461721035176943TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC22100.13995039043625893TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCACTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT20570.13026151725221022TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC16610.10518443371702539TruSeq Adapter, Index 22 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGG302.1660198E-645.0000042
GCGATAA207.034089E-445.010
TAAGCGA207.034089E-445.017
TCGTTAG2200.043.9772721
TACGGGT1200.043.1250044
CGTTAGG3800.042.631582
GCGATGT3550.041.8309869
TTAGGGA24900.040.9337354
CATATCG556.184564E-1140.90909232
TTTACGG7500.040.82
TATGGGA20550.040.2919734
TTACGGG12900.040.2906953
TTTTACG4700.040.2127651
TTATGGG41850.039.892473
ACACGCG1700.039.70588336
AACGGGG1250.039.6000024
TAGGGAA11950.039.539755
TAGGGCG6500.039.461545
CGAATAT1200.039.37500414
CTAGGGA13600.039.3754
Produced by FastQC (version 0.11.5)