##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934146.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1579131
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.908020930499116	33.0	31.0	34.0	30.0	34.0
2	32.13034320775161	33.0	31.0	34.0	30.0	34.0
3	32.22042693101459	34.0	31.0	34.0	30.0	34.0
4	35.65722159846143	37.0	35.0	37.0	33.0	37.0
5	29.41283275421735	37.0	35.0	37.0	0.0	37.0
6	23.656702325519543	35.0	10.0	37.0	0.0	37.0
7	30.36120055904165	35.0	19.0	37.0	17.0	37.0
8	34.24986084118417	35.0	32.0	37.0	32.0	37.0
9	36.85745577789303	38.0	37.0	39.0	34.0	39.0
10	36.9816728314497	39.0	37.0	39.0	34.0	39.0
11	37.113307255699496	39.0	37.0	39.0	34.0	39.0
12	36.892566227881034	39.0	35.0	39.0	33.0	39.0
13	36.693124256315656	39.0	35.0	39.0	33.0	39.0
14	37.253083499722315	40.0	36.0	41.0	31.0	41.0
15	37.283521759752674	40.0	36.0	41.0	31.0	41.0
16	37.69319201510198	40.0	36.0	41.0	33.0	41.0
17	37.809806152877755	40.0	36.0	41.0	33.0	41.0
18	37.91901558515411	40.0	36.0	41.0	33.0	41.0
19	37.928304238217095	40.0	36.0	41.0	33.0	41.0
20	37.826235442151415	40.0	36.0	41.0	33.0	41.0
21	37.72744693125523	40.0	36.0	41.0	33.0	41.0
22	37.90425366863167	40.0	36.0	41.0	34.0	41.0
23	37.89629676068673	40.0	36.0	41.0	34.0	41.0
24	37.84916894165209	40.0	35.0	41.0	34.0	41.0
25	37.57324946442062	39.0	35.0	41.0	33.0	41.0
26	37.46503298333071	39.0	35.0	41.0	33.0	41.0
27	36.64356725312846	39.0	35.0	41.0	32.0	41.0
28	37.01164374583236	39.0	35.0	41.0	32.0	41.0
29	37.27719739527626	39.0	35.0	41.0	32.0	41.0
30	37.3183225457546	39.0	35.0	41.0	33.0	41.0
31	37.28259276779444	39.0	35.0	41.0	32.0	41.0
32	37.331888234731636	39.0	35.0	41.0	33.0	41.0
33	36.15905900143813	39.0	35.0	41.0	31.0	41.0
34	36.578245883337104	39.0	35.0	41.0	31.0	41.0
35	36.84566574907338	39.0	35.0	41.0	31.0	41.0
36	36.99472241378328	39.0	35.0	41.0	32.0	41.0
37	37.124329140520956	39.0	35.0	41.0	32.0	41.0
38	37.08923198898635	39.0	35.0	41.0	32.0	41.0
39	37.10862240054815	39.0	35.0	41.0	32.0	41.0
40	37.0304749890921	39.0	35.0	41.0	32.0	41.0
41	36.887990293395546	39.0	35.0	40.0	32.0	41.0
42	36.94619319106521	39.0	35.0	40.0	32.0	41.0
43	36.85396968332583	39.0	35.0	40.0	32.0	41.0
44	36.89080639921577	39.0	35.0	40.0	32.0	41.0
45	36.845117979445654	39.0	35.0	40.0	32.0	41.0
46	36.82101548256605	39.0	35.0	40.0	32.0	41.0
47	36.772132267683936	39.0	35.0	40.0	32.0	41.0
48	36.71596340012323	38.0	35.0	40.0	32.0	41.0
49	36.757461540556164	39.0	35.0	40.0	32.0	41.0
50	36.66747217298628	38.0	35.0	40.0	32.0	41.0
51	35.855647821491694	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	13.0
11	10.0
12	17.0
13	19.0
14	28.0
15	36.0
16	61.0
17	116.0
18	259.0
19	420.0
20	771.0
21	1138.0
22	1927.0
23	3083.0
24	4616.0
25	6928.0
26	9222.0
27	11971.0
28	16160.0
29	21109.0
30	29738.0
31	42160.0
32	62491.0
33	102558.0
34	132839.0
35	138764.0
36	178099.0
37	264925.0
38	299673.0
39	249828.0
40	143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.5905697500714	4.4945606159337	55.05888998442815	16.855979649566756
2	20.727792691043366	4.290650997289015	52.63692499229007	22.344631319377555
3	22.226591714050322	3.8464193280988086	51.17650150620816	22.750487451642705
4	20.83310377669744	4.680675637423367	49.14861401618992	25.33760656968928
5	15.16631615743089	22.72021763868862	40.809723829118674	21.303742374761818
6	17.002642592666472	29.48007480063402	39.17344412844786	14.343838478251648
7	80.97725901144364	2.2367365342077385	10.731091974003423	6.054912480345203
8	82.01282857470343	2.4605305069687065	9.157188352327958	6.369452565999907
9	76.3409115519865	5.1292767984416745	12.052768263051007	6.477043386520815
10	43.67294416992637	22.723320611146256	18.741067080565195	14.862668138362176
11	33.748181753128776	21.979050503093156	24.307989647470666	19.964778096307402
12	30.39779473647215	18.731378207381148	30.42483492503155	20.44599213111515
13	29.35316955971354	19.376226544852834	31.873669758873707	19.396934136559917
14	21.22274846102065	21.850245483116982	36.0233571502301	20.903648905632274
15	18.647597951024963	21.729229557269157	38.43810298195653	21.18506950974935
16	25.51289285056148	21.43286402458061	29.889223883262378	23.165019241595537
17	26.34714915988604	21.535198789714087	27.98159240746968	24.136059642930192
18	26.446634256435974	20.16799112929833	31.282711820615262	22.10266279365043
19	26.361714132646373	23.000245071498185	29.17674341140792	21.461297384447523
20	22.86992022827745	29.2255677331393	29.089796856625576	18.814715181957673
21	22.77949074522633	24.76210016775049	33.2292254410812	19.229183645941976
22	20.79707130060774	21.565658580573746	34.296964596350776	23.340305522467737
23	20.496399602059615	22.89822693620732	32.51693494713231	24.088438514600753
24	22.02686160932817	26.063512146870654	28.036496022179286	23.873130221621892
25	24.117695112058467	24.97994149947028	25.14965509511244	25.75270829335882
26	19.309797603872003	24.6941514035251	27.185964938944267	28.810086053658623
27	19.47070888988944	24.325784244625684	30.911494993132298	25.292011872352578
28	19.11342377548158	23.256968547891212	28.675518370546836	28.954089306080373
29	22.001404569981844	25.237994821202292	27.09762521285441	25.662975395961453
30	25.723261717995534	22.139328529425363	28.100518576356237	24.03689117622287
31	23.89611754819581	23.553967340264993	29.65517110360065	22.894744007938545
32	26.75921123706646	24.225095954673804	25.817427433189522	23.19826537507021
33	25.25015340715875	25.258955716783472	24.875326999469962	24.61556387658782
34	21.114651032751556	22.832431254911718	31.630877995555785	24.422039716780937
35	20.20826644527908	27.411722016729456	27.45763334390877	24.922378194082697
36	23.734129720713483	26.653963477380916	25.500670938636503	24.1112358632691
37	22.797475320286917	26.73103118107364	27.460039730712648	23.01145376792679
38	25.962570553044678	23.961596599648793	26.447394168058253	23.62843867924827
39	22.713125130214024	21.810540100852936	27.633996166245865	27.84233860268717
40	23.668840647166068	20.575367084808036	31.013766432297256	24.742025835728636
41	19.695769382020874	22.262054256423312	29.72476634300764	28.317410018548177
42	19.44297211567628	23.193515927430973	31.725043710749773	25.638468246142978
43	21.303362418950673	22.240333449219857	30.341624602392077	26.114679529437396
44	22.60781404455995	20.666176523670295	28.89443624373152	27.831573188038234
45	21.806993846615637	20.012842506416504	26.88959940625572	31.290564240712136
46	21.326539723430166	20.51134453063109	30.878502163531714	27.283613582407035
47	17.835949012463185	23.221252701644133	34.07817337510314	24.864624910789544
48	19.232666574210754	25.097664474954897	30.58536625523785	25.0843026955965
49	23.927590554551838	20.689923761866496	31.138898546099085	24.243587137482578
50	21.12237680091139	21.10401227003966	28.69432618319823	29.079284745850725
51	19.101708471304786	22.118367633844183	27.390887773085325	31.389036121765706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	302.0
1	1020.0
2	1738.0
3	3203.0
4	4668.0
5	3215.5
6	1763.0
7	1825.0
8	1887.0
9	2085.0
10	2283.0
11	2404.5
12	2526.0
13	2452.5
14	2379.0
15	2302.0
16	2225.0
17	2287.0
18	2349.0
19	2555.0
20	2761.0
21	2876.0
22	2991.0
23	3987.0
24	4983.0
25	5944.5
26	8812.0
27	10718.0
28	12366.5
29	14015.0
30	16473.5
31	18932.0
32	22113.0
33	25294.0
34	29317.0
35	33340.0
36	34674.5
37	36009.0
38	39942.0
39	43875.0
40	50663.5
41	57452.0
42	66548.5
43	75645.0
44	88604.0
45	101563.0
46	126070.5
47	150578.0
48	177150.0
49	203722.0
50	196997.0
51	190272.0
52	165359.0
53	140446.0
54	125422.0
55	110398.0
56	100294.0
57	90190.0
58	80050.0
59	69910.0
60	58578.5
61	47247.0
62	41977.0
63	36707.0
64	31936.5
65	27166.0
66	23688.0
67	20210.0
68	16404.0
69	12598.0
70	11018.5
71	9439.0
72	7691.0
73	5943.0
74	4722.5
75	2841.5
76	2181.0
77	1657.0
78	1133.0
79	812.5
80	492.0
81	388.0
82	284.0
83	163.5
84	43.0
85	37.5
86	32.0
87	32.0
88	32.0
89	17.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1579131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.93643905811596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.77682130475603	24.71594047429296
2	12.76945640261437	9.177775836356398
3	5.0640174920681496	5.459482679788209
4	2.7630155071268234	3.9717175355396974
5	1.7059730561108883	3.065329838285839
6	1.2074109947978262	2.603403097959075
7	0.9330834282734549	2.3472187029400846
8	0.7597939574174287	2.1843431397964945
9	0.6457160511408799	2.088426196860437
>10	5.179205721274346	33.48865539874167
>50	0.12064261658351577	2.9613913609886975
>100	0.06831043815388592	4.538015492794992
>500	0.0037729564838334317	0.94668005835379
>1k	0.002382919884526378	1.6108404158917609
>5k	3.971533140877297E-4	0.8407797714099466
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	6411	0.4059827842021973	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	5371	0.34012377693807544	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	4139	0.26210618371750033	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	3547	0.22461721035176943	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTATCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2210	0.13995039043625893	TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCACTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	2057	0.13026151725221022	TruSeq Adapter, Index 22 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	1661	0.10518443371702539	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3860984300859143	0.0
2	0.0	0.0	0.0	1.429077131662921	0.0
3	0.0	0.0	0.0	1.9814062291222196	0.0
4	0.0	0.0	0.0	2.5660948964968706	0.0
5	0.0	0.0	0.0	3.572977796015657	0.0
6	0.0	0.0	0.0	4.363285883185119	0.0
7	0.0	0.0	0.0	5.412597181614445	0.0
8	0.0	0.0	0.0	6.9621836313770045	0.0
9	0.0	0.0	0.0	7.677640423752051	0.0
10	0.0	0.0	0.0	9.002166381383178	0.0
11	6.332596852319408E-5	0.0	0.0	11.655967744284673	0.0
12	6.332596852319408E-5	0.0	0.0	13.961349628371554	0.0
13	6.332596852319408E-5	0.0	0.0	14.7355095935676	0.0
14	6.332596852319408E-5	0.0	0.0	15.056635579948718	0.0
15	6.332596852319408E-5	0.0	0.0	15.51403905059175	0.0
16	6.332596852319408E-5	0.0	0.0	16.427579472507347	0.0
17	6.332596852319408E-5	0.0	0.0	17.641095007317315	0.0
18	6.332596852319408E-5	0.0	0.0	18.936491019427773	0.0
19	6.332596852319408E-5	0.0	0.0	19.854844214951136	0.0
20	6.332596852319408E-5	0.0	0.0	20.85520453971203	0.0
21	6.332596852319408E-5	0.0	0.0	22.188406155030837	0.0
22	6.332596852319408E-5	0.0	0.0	23.59791556242009	0.0
23	6.332596852319408E-5	0.0	0.0	24.81833362779909	0.0
24	6.332596852319408E-5	0.0	0.0	25.788170835731805	0.0
25	6.332596852319408E-5	0.0	0.0	26.636738813942607	0.0
26	6.332596852319408E-5	0.0	0.0	27.394687331196714	0.0
27	6.332596852319408E-5	0.0	0.0	28.33311485874193	0.0
28	6.332596852319408E-5	0.0	0.0	29.15369275886548	0.0
29	6.332596852319408E-5	0.0	0.0	30.133662121761905	0.0
30	6.332596852319408E-5	0.0	0.0	31.159606137806172	0.0
31	6.332596852319408E-5	0.0	0.0	31.94858437963665	0.0
32	6.332596852319408E-5	0.0	0.0	32.713815383270926	0.0
33	6.332596852319408E-5	0.0	0.0	33.46473471801896	0.0
34	1.2665193704638816E-4	0.0	0.0	34.34958847619355	0.0
35	1.2665193704638816E-4	0.0	0.0	35.31328306517952	0.0
36	1.2665193704638816E-4	0.0	0.0	36.09535877644097	0.0
37	1.2665193704638816E-4	0.0	0.0	36.97128357305379	0.0
38	1.2665193704638816E-4	0.0	0.0	38.09449627674968	0.0
39	1.2665193704638816E-4	0.0	0.0	40.39145580702298	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACGG	30	2.1660198E-6	45.000004	2
GCGATAA	20	7.034089E-4	45.0	10
TAAGCGA	20	7.034089E-4	45.0	17
TCGTTAG	220	0.0	43.977272	1
TACGGGT	120	0.0	43.125004	4
CGTTAGG	380	0.0	42.63158	2
GCGATGT	355	0.0	41.830986	9
TTAGGGA	2490	0.0	40.933735	4
CATATCG	55	6.184564E-11	40.909092	32
TTTACGG	750	0.0	40.8	2
TATGGGA	2055	0.0	40.291973	4
TTACGGG	1290	0.0	40.290695	3
TTTTACG	470	0.0	40.212765	1
TTATGGG	4185	0.0	39.89247	3
ACACGCG	170	0.0	39.705883	36
AACGGGG	125	0.0	39.600002	4
TAGGGAA	1195	0.0	39.53975	5
TAGGGCG	650	0.0	39.46154	5
CGAATAT	120	0.0	39.375004	14
CTAGGGA	1360	0.0	39.375	4
>>END_MODULE