FastQCFastQC Report
Sat 14 Jan 2017
SRR2934145.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934145.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431192
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGC103570.7236625134852627No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCG81130.5668701334272411No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC61360.42873353121034774Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC44210.308903347698981Illumina Single End Adapter 1 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC41540.2902475698578527Illumina Single End Adapter 1 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT41090.2871033376374379Illumina Single End Adapter 1 (96% over 25bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAAGAACT32320.22582574525290808No Hit
TCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC30090.21024432780507438Illumina Single End Adapter 1 (95% over 24bp)
GAATCAGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTC24040.16797187239727443Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCT21110.14749942705101762No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTC20350.1421891681898725No Hit
GCTGTATCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC18950.13240711239302624Illumina Single End Adapter 1 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAGAACTCGTA16450.11493915561294361No Hit
CCTGTATCTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGC16010.11186479521964907Illumina Single End Adapter 1 (95% over 24bp)
CTGTCACTTATACACATCTGACGCGCAAGAACTCGTATGCCGTCTTCTGCT15680.10955902492467817Illumina Single End Adapter 1 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGAA253.891287E-545.018
AATACGG3500.043.0714265
TAATACG3650.042.534254
CGATGAA5750.041.86956419
CGTTAGG2650.041.6037752
AGGGCGA7800.041.538466
TTAGGGA27450.041.147544
TTATGGG31750.040.6771623
TATGGGA20750.040.662654
TACGAAT1000.040.512
CTTACGG2350.040.2127652
CCGATGA5800.039.95689418
TTACGGG11550.039.5454563
TAGGGAT13950.039.516135
ACGCAAA403.4590084E-739.37500427
TATGGGC10000.039.154
TCGTTAG1500.039.0000041
ATAACGA750.039.00000418
TTCGGGC3750.039.04
GGGCGAT27550.038.874777
Produced by FastQC (version 0.11.5)