##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934143.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720771
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896449773922647	33.0	31.0	34.0	30.0	34.0
2	32.107494613407034	33.0	31.0	34.0	30.0	34.0
3	32.18420274955568	34.0	31.0	34.0	30.0	34.0
4	35.64310023571981	37.0	35.0	37.0	33.0	37.0
5	29.218771287968025	37.0	35.0	37.0	0.0	37.0
6	23.343107588956826	35.0	0.0	37.0	0.0	37.0
7	30.24451871676302	35.0	19.0	37.0	17.0	37.0
8	34.2255071305588	35.0	32.0	37.0	32.0	37.0
9	36.884137957825715	38.0	37.0	39.0	34.0	39.0
10	36.89165490842445	39.0	37.0	39.0	33.0	39.0
11	37.06840452792912	39.0	37.0	39.0	34.0	39.0
12	37.09364416714879	39.0	37.0	39.0	34.0	39.0
13	37.023637188510634	39.0	37.0	39.0	33.0	39.0
14	37.96660936691404	40.0	37.0	41.0	33.0	41.0
15	37.88521597012088	40.0	37.0	41.0	33.0	41.0
16	38.16339863840249	40.0	37.0	41.0	33.0	41.0
17	38.204118922653656	40.0	37.0	41.0	33.0	41.0
18	38.28389876951209	40.0	37.0	41.0	34.0	41.0
19	38.287325655443965	40.0	37.0	41.0	34.0	41.0
20	38.190669435923475	40.0	37.0	41.0	34.0	41.0
21	38.09708076490314	40.0	37.0	41.0	34.0	41.0
22	38.219025182755686	40.0	37.0	41.0	34.0	41.0
23	38.16260365636242	40.0	37.0	41.0	34.0	41.0
24	38.143837640526606	40.0	37.0	41.0	34.0	41.0
25	37.90477835539998	40.0	36.0	41.0	34.0	41.0
26	37.78183639463852	40.0	36.0	41.0	33.0	41.0
27	36.91197481585691	40.0	36.0	41.0	32.0	41.0
28	37.30609860829584	40.0	36.0	41.0	32.0	41.0
29	37.62595193202834	40.0	36.0	41.0	33.0	41.0
30	37.61313232635608	40.0	36.0	41.0	33.0	41.0
31	37.624357805738576	40.0	36.0	41.0	33.0	41.0
32	37.64199447536041	40.0	36.0	41.0	33.0	41.0
33	36.36579579367094	39.0	35.0	41.0	31.0	41.0
34	36.87768792029646	40.0	35.0	41.0	31.0	41.0
35	37.1861839613414	40.0	35.0	41.0	31.0	41.0
36	37.27598640899814	39.0	35.0	41.0	32.0	41.0
37	37.386075466410276	39.0	36.0	41.0	33.0	41.0
38	37.36753837210431	39.0	36.0	41.0	33.0	41.0
39	37.408937096525804	40.0	36.0	41.0	33.0	41.0
40	37.37105405184171	40.0	36.0	41.0	33.0	41.0
41	37.251880278202094	39.0	35.0	41.0	32.0	41.0
42	37.273945538874344	39.0	35.0	41.0	33.0	41.0
43	37.17024547325017	39.0	35.0	41.0	32.0	41.0
44	37.23111501433881	39.0	35.0	41.0	32.0	41.0
45	37.18365195048081	39.0	35.0	41.0	32.0	41.0
46	37.137462522770754	39.0	35.0	41.0	32.0	41.0
47	37.07782915794337	39.0	35.0	41.0	32.0	41.0
48	37.01910038000974	39.0	35.0	41.0	32.0	41.0
49	37.05676560238966	39.0	35.0	41.0	32.0	41.0
50	36.96618204672497	39.0	35.0	40.0	32.0	41.0
51	36.21161783701064	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	5.0
11	4.0
12	7.0
13	5.0
14	10.0
15	15.0
16	35.0
17	31.0
18	90.0
19	162.0
20	290.0
21	536.0
22	945.0
23	1440.0
24	2153.0
25	3295.0
26	4185.0
27	5111.0
28	6443.0
29	8374.0
30	11577.0
31	16320.0
32	23720.0
33	37257.0
34	50499.0
35	61461.0
36	85020.0
37	128639.0
38	147942.0
39	125118.0
40	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.470879100296766	4.304695943649231	55.46588306133293	15.75854189472107
2	20.006909267992192	4.951087099786202	53.024330890116275	22.01767274210533
3	20.368605285173793	5.01671127167991	52.91098004775442	21.70370339539188
4	18.603967140742345	4.46938070482858	52.25890053845119	24.66775161597789
5	14.951628187038601	22.93516248572709	41.670239229935724	20.442970097298588
6	15.207604079520404	30.299221250577506	40.9482345987838	13.544940071118289
7	81.39409049476185	1.873271815874945	11.488947252317312	5.243690437045886
8	82.7221128486024	2.629545306345566	8.620629853309858	6.027711991742176
9	77.35383360318325	4.637117753072751	11.322320126642166	6.686728517101825
10	38.37446290153183	23.491649913772893	21.10406772747516	17.029819457220118
11	28.318148205185835	24.166760316383428	25.94582745421223	21.56926402421851
12	23.946024465468227	21.25182616947685	32.036527551746666	22.765621813308247
13	24.045362535396126	22.483562740454317	34.4676741988787	19.003400525270855
14	20.34432572897633	24.363355351422296	34.84116314335621	20.45115577624516
15	18.583849794178732	24.063121296500555	36.42807493642225	20.924953972898468
16	21.1688594574421	23.613880136686966	31.865183255153163	23.352077150717772
17	21.953574713744032	24.8640691703745	29.180974262283026	24.00138185359844
18	22.551129276843824	23.12828901273775	31.886826745249188	22.433754965169243
19	22.72025372829928	25.769072285094712	29.474687522111736	22.03598646449427
20	22.021279990454666	27.93841594625755	30.103597397786537	19.936706665501248
21	22.110212536297936	26.956273212989977	31.088931158440058	19.844583092272025
22	20.62832716632606	24.09947126063618	30.966284714562605	24.30591685847516
23	19.47997352834673	26.056126009509263	30.24053409474022	24.223366367403795
24	21.076319663249492	26.174610243752873	29.16141187700393	23.587658215993706
25	21.741024541775406	26.54088469153171	26.510778041846855	25.20731272484603
26	20.373322456092158	26.12938089906503	28.397923889834637	25.099372755008183
27	20.12039885067518	27.509847094292084	29.96763188308076	22.40212217195198
28	18.62713677437078	24.45561766497265	31.41497091309168	25.5022746475649
29	20.509704191761323	24.199503032169716	30.19752459519043	25.093268180878532
30	22.57818363946385	25.28819833206386	29.2243999827962	22.909218045676088
31	21.436212056256426	25.369500160245074	29.051113321706897	24.14317446179161
32	23.213475569910553	25.971078192657586	27.375546463439843	23.439899773992018
33	21.5834155369736	27.984338992551034	25.86743917277471	24.564806297700656
34	20.14065493755992	23.898297795000076	29.755359191754383	26.205688075685618
35	21.50738584099527	25.53612728592022	27.77955827856559	25.176928594518927
36	22.906165758611266	27.57047661462517	26.19223026453617	23.33112736222739
37	19.223442674580415	27.85031584234105	30.181291977618415	22.744949505460124
38	20.452404439135314	25.626724715617026	30.139808621601034	23.781062223646625
39	19.906461275495268	23.59473397237125	29.34954375245397	27.14926099967951
40	22.563615905745376	21.939284460667814	28.558307701059004	26.938791932527806
41	21.911397656121014	24.07477548347533	25.915582064206244	28.09824479619741
42	22.095367321937204	25.132254211115594	28.69788046411412	24.074498002833078
43	20.89776086995731	24.697442044699354	30.499978495250225	23.904818590093107
44	20.589202395767867	22.805301545150957	29.72386513885825	26.881630920222925
45	21.14125013353756	21.713415217870864	28.286654152289703	28.858680496301876
46	22.313467106750963	22.38602829470109	29.230782037568105	26.06972256097984
47	19.032952213671194	23.879012890363235	32.32288757455558	24.765147321409987
48	18.841768051156333	24.88279911372683	30.91841930377332	25.35701353134352
49	21.560662124308553	21.742550685307815	32.18955812595124	24.507229064432394
50	20.76346023910507	20.948678567811413	29.7911541946055	28.496706998478018
51	19.655063813610703	21.34838943298218	27.22986912625508	31.766677627152035
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	298.5
2	511.0
3	1717.0
4	2923.0
5	1994.5
6	1066.0
7	1052.0
8	1038.0
9	1112.0
10	1186.0
11	1208.5
12	1231.0
13	1249.5
14	1268.0
15	1334.5
16	1401.0
17	1361.0
18	1321.0
19	1408.0
20	1495.0
21	1699.0
22	1903.0
23	2055.0
24	2207.0
25	2723.5
26	4196.0
27	5152.0
28	5942.5
29	6733.0
30	8105.0
31	9477.0
32	11553.5
33	13630.0
34	15920.5
35	18211.0
36	20214.5
37	22218.0
38	24708.5
39	27199.0
40	30179.5
41	33160.0
42	39039.0
43	44918.0
44	51655.0
45	58392.0
46	70987.0
47	83582.0
48	90131.5
49	96681.0
50	89967.5
51	83254.0
52	69579.5
53	55905.0
54	47737.5
55	39570.0
56	34789.0
57	30008.0
58	26072.0
59	22136.0
60	18906.0
61	15676.0
62	13430.5
63	11185.0
64	9600.0
65	8015.0
66	6761.0
67	5507.0
68	4473.5
69	3440.0
70	2869.0
71	2298.0
72	1909.0
73	1520.0
74	1279.5
75	761.0
76	483.0
77	371.5
78	260.0
79	208.5
80	157.0
81	104.5
82	52.0
83	39.5
84	27.0
85	17.5
86	8.0
87	5.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	720771.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.349483424301155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.5924985647422	38.816357520517386
2	10.719986066914524	11.009314937035333
3	4.286635834572706	6.603496072002195
4	2.4857961039487453	5.105773833436339
5	1.6565878782416457	4.25324658973338
6	1.1885337367243765	3.6618356047890654
7	0.8937351527275255	3.2124986887488127
8	0.6801696490061752	2.7941088093884265
9	0.5136170818551362	2.3736574648042423
>10	1.894949521009696	14.682072289198143
>50	0.05355988444515008	1.8652885683545382
>100	0.028883009575621107	2.780216321820386
>500	0.0030845932556111725	1.1833342517383405
>1k	0.0019629229808434735	1.6587990484334003
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	2607	0.3616960171816014	No Hit
CTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGCT	2205	0.30592240808800575	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	1669	0.23155759596321163	No Hit
GCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	1530	0.21227269132637136	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGC	1310	0.18174982067813494	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTC	1117	0.1549729387003639	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCG	1082	0.15011702746087174	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	984	0.13652047599029374	No Hit
CCTGTATCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGC	915	0.12694739383243778	No Hit
CTGTCACTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTGCT	914	0.1268086535113094	Illumina Single End Adapter 1 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCCGTCTTCTG	805	0.11168595850831957	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTCCGATCGTATGCC	778	0.10793996983785419	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.387403211283473E-4	0.0	0.0	0.32492983208258935	0.0
2	1.387403211283473E-4	0.0	0.0	1.2034335454672844	0.0
3	1.387403211283473E-4	0.0	0.0	1.7123330433660622	0.0
4	1.387403211283473E-4	0.0	0.0	2.2972622372431744	0.0
5	1.387403211283473E-4	0.0	0.0	3.4270246721913065	0.0
6	1.387403211283473E-4	0.0	0.0	4.388078876647368	0.0
7	1.387403211283473E-4	0.0	0.0	5.547947961280351	0.0
8	1.387403211283473E-4	0.0	0.0	7.439117278580853	0.0
9	1.387403211283473E-4	0.0	0.0	8.281271027829922	0.0
10	1.387403211283473E-4	0.0	0.0	9.886635283606028	0.0
11	1.387403211283473E-4	0.0	0.0	12.53407809137715	0.0
12	1.387403211283473E-4	0.0	0.0	14.928874774373552	0.0
13	1.387403211283473E-4	0.0	0.0	15.715948616134668	0.0
14	1.387403211283473E-4	0.0	0.0	16.012297942064816	0.0
15	1.387403211283473E-4	0.0	0.0	16.398550996086136	0.0
16	1.387403211283473E-4	0.0	0.0	17.24764176139162	0.0
17	1.387403211283473E-4	0.0	0.0	18.461342090622402	0.0
18	1.387403211283473E-4	0.0	0.0	19.74663242555541	0.0
19	1.387403211283473E-4	0.0	0.0	20.69880724945926	0.0
20	1.387403211283473E-4	0.0	0.0	21.626147555881133	0.0
21	1.387403211283473E-4	0.0	0.0	22.973316074037385	0.0
22	1.387403211283473E-4	0.0	0.0	24.393739481749403	0.0
23	1.387403211283473E-4	0.0	0.0	25.77476063826097	0.0
24	1.387403211283473E-4	0.0	0.0	26.816422969292606	0.0
25	1.387403211283473E-4	0.0	0.0	27.711436780891574	0.0
26	1.387403211283473E-4	0.0	0.0	28.513772057976805	0.0
27	1.387403211283473E-4	0.0	0.0	29.40226507448274	0.0
28	1.387403211283473E-4	0.0	0.0	30.268559639608142	0.0
29	1.387403211283473E-4	0.0	0.0	31.175782599466405	0.0
30	1.387403211283473E-4	0.0	0.0	32.11214102676162	0.0
31	1.387403211283473E-4	0.0	0.0	32.93972704229221	0.0
32	1.387403211283473E-4	0.0	0.0	33.76939416263973	0.0
33	1.387403211283473E-4	0.0	0.0	34.57297810261512	0.0
34	1.387403211283473E-4	0.0	0.0	35.41943280181916	0.0
35	1.387403211283473E-4	0.0	0.0	36.320273706905525	0.0
36	1.387403211283473E-4	0.0	0.0	37.14661105954596	0.0
37	1.387403211283473E-4	0.0	0.0	38.013183105313615	0.0
38	1.387403211283473E-4	0.0	0.0	38.93760986499179	0.0
39	1.387403211283473E-4	0.0	0.0	40.29504516691154	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAT	30	2.16437E-6	45.000004	14
TAATACG	35	1.2110831E-7	45.0	4
ATCTCGC	20	7.031535E-4	45.0	12
ATAGGCG	20	7.031535E-4	45.0	37
AACGGGG	35	1.2110831E-7	45.0	4
ACGATCG	45	3.8380676E-10	45.0	33
TTAACGG	20	7.031535E-4	45.0	2
TTTATCG	20	7.031535E-4	45.0	2
CGTTATT	215	0.0	43.953487	1
CGTTTAT	355	0.0	42.464787	1
TAACGGG	80	0.0	42.1875	3
TAGGGCG	305	0.0	42.049183	5
AGGGCGA	455	0.0	42.032967	6
TCGTTTG	120	0.0	41.250004	1
CTTTACG	115	0.0	41.086956	1
TATGGGT	490	0.0	40.867348	4
TGTCGAG	50	1.0804797E-9	40.5	1
TTAGGGT	725	0.0	40.34483	4
CGTTCTG	95	0.0	40.263157	1
CGTTAGG	180	0.0	40.0	2
>>END_MODULE