FastQCFastQC Report
Sat 14 Jan 2017
SRR2934141.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934141.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1867971
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46870.25091395958502566No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT44730.2394576789468359No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC42440.22719838798353936No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC30440.16295756197499853No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC25090.1343168603795241No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG24090.1289634582121457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT19000.044.289471
CGTTTTT38000.042.4539451
CGTTATT13700.041.7153321
GTTTACG1250.041.41
GCGTTAG1200.041.2499961
CCGTTAG556.184564E-1140.9090921
ATGGGCG7300.040.684935
GCGTACG501.0822987E-940.5000041
TCTACGG4900.039.948982
TAGGGCG11700.039.8076935
AGGGCGA13600.039.540446
CGTAGAC403.4597724E-739.37515
CCGTAGG1200.039.3749962
TTATGGG54600.039.271983
TAGGGCA14300.039.0209775
ACGGGAG5150.038.8834955
CTAGGGT10200.038.823534
TAGGGTA13800.038.8043485
TTAGGGA32750.038.7480934
CGCATGG2150.038.7209322
Produced by FastQC (version 0.11.5)