##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934141.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1867971
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90324314456702	33.0	31.0	34.0	30.0	34.0
2	32.12792703955254	33.0	31.0	34.0	30.0	34.0
3	32.19517273019763	33.0	31.0	34.0	30.0	34.0
4	35.659103380084595	37.0	35.0	37.0	33.0	37.0
5	29.184725030527776	37.0	35.0	37.0	0.0	37.0
6	23.432917855791125	35.0	10.0	37.0	0.0	37.0
7	30.302238632184334	35.0	19.0	37.0	17.0	37.0
8	34.29816897585669	35.0	32.0	37.0	32.0	37.0
9	36.922436697357725	38.0	37.0	39.0	34.0	39.0
10	36.8704813939831	39.0	37.0	39.0	33.0	39.0
11	37.05391304254724	39.0	37.0	39.0	34.0	39.0
12	37.1634693472222	39.0	37.0	39.0	34.0	39.0
13	37.166695307368265	39.0	37.0	39.0	34.0	39.0
14	38.237337731688555	40.0	38.0	41.0	33.0	41.0
15	38.11838620620984	40.0	38.0	41.0	33.0	41.0
16	38.32757949668383	40.0	38.0	41.0	34.0	41.0
17	38.34150476640162	40.0	38.0	41.0	34.0	41.0
18	38.38419225994408	40.0	38.0	41.0	34.0	41.0
19	38.35885246612501	40.0	37.0	41.0	34.0	41.0
20	38.281930501062384	40.0	37.0	41.0	34.0	41.0
21	38.12521607669498	40.0	37.0	41.0	34.0	41.0
22	38.199251487308956	40.0	37.0	41.0	34.0	41.0
23	38.12493716444206	40.0	37.0	41.0	34.0	41.0
24	38.10138968966863	40.0	37.0	41.0	34.0	41.0
25	37.8781640614335	40.0	36.0	41.0	33.0	41.0
26	37.79060167422299	40.0	36.0	41.0	33.0	41.0
27	36.93953814058141	40.0	36.0	41.0	32.0	41.0
28	37.31837325097659	40.0	36.0	41.0	32.0	41.0
29	37.61052607347759	40.0	36.0	41.0	33.0	41.0
30	37.59290213820236	40.0	36.0	41.0	33.0	41.0
31	37.59057876166172	40.0	36.0	41.0	33.0	41.0
32	37.5596339557734	40.0	36.0	41.0	33.0	41.0
33	36.241179868424084	40.0	35.0	41.0	30.0	41.0
34	36.6730934259686	40.0	36.0	41.0	30.0	41.0
35	36.944933834625914	40.0	35.0	41.0	30.0	41.0
36	37.05875465946741	40.0	36.0	41.0	32.0	41.0
37	37.16192167865561	40.0	36.0	41.0	32.0	41.0
38	37.10701504466611	40.0	36.0	41.0	32.0	41.0
39	37.09093074785422	40.0	36.0	41.0	32.0	41.0
40	37.043018333796404	40.0	36.0	41.0	32.0	41.0
41	36.96334097263823	39.0	35.0	41.0	32.0	41.0
42	37.01474755229069	40.0	35.0	41.0	32.0	41.0
43	36.915558646253075	39.0	35.0	41.0	32.0	41.0
44	36.944185429002914	39.0	35.0	41.0	32.0	41.0
45	36.86608250342216	39.0	35.0	41.0	32.0	41.0
46	36.82715577490229	39.0	35.0	41.0	32.0	41.0
47	36.790856496166164	39.0	35.0	41.0	31.0	41.0
48	36.72541597273191	39.0	35.0	41.0	31.0	41.0
49	36.74464325195627	39.0	35.0	41.0	31.0	41.0
50	36.666653818501466	39.0	35.0	41.0	31.0	41.0
51	35.93918749273945	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	7.0
10	18.0
11	14.0
12	26.0
13	21.0
14	29.0
15	59.0
16	93.0
17	168.0
18	303.0
19	599.0
20	1073.0
21	2011.0
22	3427.0
23	5460.0
24	8922.0
25	13454.0
26	16461.0
27	17335.0
28	18451.0
29	21703.0
30	28772.0
31	39320.0
32	55928.0
33	87585.0
34	121652.0
35	155893.0
36	217510.0
37	334849.0
38	383900.0
39	332760.0
40	162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.30400204285827	4.342144497960621	55.61890414786953	16.734949311311578
2	22.315228662543475	4.050062875708456	51.70176624797709	21.932942213770986
3	19.86171091521228	4.070031065792777	53.92995929808332	22.13829872091162
4	17.95145641982665	4.339039524703542	52.7360435467146	24.97346050875522
5	14.130090884708595	23.058120281310575	43.404099956583906	19.407688877396918
6	15.435785673332186	29.737506631526934	41.56563458426282	13.26107311087806
7	81.68472636887832	1.8858965155240632	11.499857331832239	4.92951978376538
8	83.80269286835824	1.915447295487992	9.353999607060281	4.9278602290934925
9	78.49425927918581	4.442788458707335	11.768223382482919	5.294728879623934
10	37.544747750366575	21.602209027870348	22.81170317954615	18.04134004221693
11	26.10072640314009	25.389312789117174	26.49409439439906	22.015866413343677
12	21.65263807628705	21.521372655142933	34.236719949078434	22.589269319491574
13	22.176575546408376	22.108480270839323	35.85505342427693	19.859890758475373
14	20.185163474165286	23.90866881766366	35.61811184434876	20.288055863822297
15	18.516240348485066	24.192666802643082	36.13749892262781	21.15359392624404
16	20.374620376868805	23.819802341685175	33.182956266451676	22.622621014994344
17	21.20862690052469	24.534267394943498	30.514071149926846	23.743034554604968
18	22.178395703145284	23.231838181642004	32.660357146872194	21.929408968340514
19	23.003783249311685	26.274444303471522	29.434611136896667	21.287161310320126
20	22.686380034807822	26.454532752382132	30.05351796146728	20.805569251342767
21	22.598048899046077	26.420645716662627	31.032066343642377	19.949239040648916
22	21.71811018479409	23.888700627579336	30.661075573443057	23.73211361418352
23	20.423015132461906	25.404623519315876	29.948430676921646	24.22393067130057
24	20.860120419428352	25.557195481086165	30.364604161413638	23.218079938071845
25	21.14599209516636	26.00222380326033	27.71354587410618	25.13823822746713
26	19.608387924651936	26.080972349142467	29.217048872814406	25.093590853391195
27	19.510152994880542	25.99617445881119	29.755922334982714	24.73775021132555
28	18.979256101941626	23.851440948494382	31.196683460289265	25.972619489274727
29	20.370819461329965	23.27760977017309	30.420547214062744	25.931023554434198
30	21.530580506870827	23.24318739423685	30.844322529632418	24.381909569259907
31	20.457758712528193	24.67222456879684	30.17059686686785	24.69941985180712
32	20.937851818898686	24.25289257702609	29.43011427907607	25.379141324999154
33	20.823342546538466	25.55023605826857	28.638239030477454	24.98818236471551
34	19.103615634289824	23.01598900625331	32.006920878321985	25.873474481134878
35	19.592970126409888	23.227020119691367	29.598746447348486	27.581263306550262
36	20.288537670017362	23.671298965562098	29.84939273682514	26.190770627595395
37	19.038250593826138	24.793425593866285	32.08267151899039	24.085652293317185
38	21.020294212276312	24.49513402509996	30.918948955845675	23.565622806778048
39	20.905999076002786	22.564215397348246	32.66303384795589	23.866751678693085
40	20.958462417243094	22.087816138473244	32.5445630579918	24.409158386291864
41	18.61897213607706	22.647728471159347	30.584521922449547	28.148777470314045
42	19.288093872977687	22.861222149594397	30.99020273869348	26.860481238734437
43	20.26359081591738	22.379683624638712	31.68989240196984	25.666833157474073
44	20.764669258783996	22.40457694471702	30.138101715711862	26.69265208078712
45	21.09775794163828	21.221046793553004	29.341515473205952	28.339679791602762
46	20.915474597839044	21.546212441199568	30.600367992864985	26.9379449680964
47	18.368379380622077	22.521066975879176	32.62063490279024	26.4899187407085
48	18.60906834206741	22.682472051225634	32.18529623853904	26.523163368167925
49	20.822378934148336	20.878375520819116	33.19473375121991	25.104511793812645
50	20.276974321335825	20.445338819499874	32.17710553322294	27.100581325941352
51	18.63385459410237	20.49566079987323	30.71787517043894	30.152609435585454
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	197.0
1	682.0
2	1167.0
3	7840.0
4	14513.0
5	10284.0
6	6055.0
7	5921.0
8	5787.0
9	6017.5
10	6248.0
11	6352.5
12	6457.0
13	6363.5
14	6270.0
15	6099.5
16	5929.0
17	5759.5
18	5590.0
19	5735.0
20	5880.0
21	6163.0
22	6446.0
23	7264.5
24	8083.0
25	9126.5
26	11348.0
27	12526.0
28	16006.5
29	19487.0
30	22568.5
31	25650.0
32	30059.5
33	34469.0
34	40925.5
35	47382.0
36	52921.5
37	58461.0
38	63997.5
39	69534.0
40	76579.5
41	83625.0
42	94492.0
43	105359.0
44	122687.5
45	140016.0
46	165445.5
47	190875.0
48	211063.5
49	231252.0
50	217854.5
51	204457.0
52	176637.0
53	148817.0
54	128343.0
55	107869.0
56	95412.0
57	82955.0
58	73275.0
59	63595.0
60	55962.5
61	48330.0
62	42046.5
63	35763.0
64	29810.0
65	23857.0
66	19423.5
67	14990.0
68	12604.0
69	10218.0
70	8926.0
71	7634.0
72	6562.5
73	5491.0
74	4619.5
75	2668.5
76	1589.0
77	1121.5
78	654.0
79	494.0
80	334.0
81	232.0
82	130.0
83	109.0
84	88.0
85	50.5
86	13.0
87	8.0
88	3.0
89	3.0
90	3.0
91	3.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1867971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.02187819407641
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.16901829728505	33.84250385748668
2	10.993207721640191	9.8986971801173
3	3.998315755494418	5.400350547759788
4	2.115137371322014	3.809098283815947
5	1.3202458496466782	2.9719973914513833
6	0.9421591790958891	2.5450665480411683
7	0.7284412252566778	2.2957054480534946
8	0.5856226381352672	2.1092664865455744
9	0.49893465804337966	2.0216677861108985
>10	3.5369379497047264	27.81544605227722
>50	0.07470143955790544	2.2840212050548567
>100	0.034267064099630304	2.6784561856378435
>500	0.0012903645935184935	0.3991101600051757
>1k	0.0017204861246913243	1.9286128676426717
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4687	0.25091395958502566	No Hit
CTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGCT	4473	0.2394576789468359	No Hit
CCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	4244	0.22719838798353936	No Hit
TCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	3044	0.16295756197499853	No Hit
GCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTGC	2509	0.1343168603795241	No Hit
CGCTGTCTCTTATACACATCTGACGCCCTGTTCTTCGTATGCCGTCTTCTG	2409	0.1289634582121457	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.353402167378402E-5	0.0	0.0	0.2561602937090565	0.0
2	5.353402167378402E-5	0.0	0.0	0.8314368906155395	0.0
3	5.353402167378402E-5	0.0	0.0	1.243059983265265	0.0
4	5.353402167378402E-5	0.0	0.0	1.6850904002256994	0.0
5	5.353402167378402E-5	0.0	0.0	2.4621367248206742	0.0
6	5.353402167378402E-5	0.0	0.0	3.4376872017820403	0.0
7	5.353402167378402E-5	0.0	0.0	4.494127585492494	0.0
8	5.353402167378402E-5	0.0	0.0	6.132857522948696	0.0
9	5.353402167378402E-5	0.0	0.0	6.966542842474535	0.0
10	5.353402167378402E-5	0.0	0.0	8.258158183397923	0.0
11	5.353402167378402E-5	0.0	0.0	10.499467068814237	0.0
12	5.353402167378402E-5	0.0	0.0	12.357793563176301	0.0
13	1.0706804334756803E-4	0.0	0.0	13.1106960439964	0.0
14	1.0706804334756803E-4	0.0	0.0	13.39421222278076	0.0
15	1.0706804334756803E-4	0.0	0.0	13.714024468259947	0.0
16	1.0706804334756803E-4	0.0	0.0	14.445031534215467	0.0
17	1.6060206502135205E-4	0.0	0.0	15.458644700586893	0.0
18	1.6060206502135205E-4	0.0	0.0	16.632645795892977	0.0
19	1.6060206502135205E-4	0.0	0.0	17.400216598651692	0.0
20	1.6060206502135205E-4	0.0	0.0	18.201567369086565	0.0
21	2.1413608669513607E-4	0.0	0.0	19.421179450858713	0.0
22	2.1413608669513607E-4	0.0	0.0	20.658778963913253	0.0
23	2.1413608669513607E-4	0.0	0.0	21.93369169007442	0.0
24	2.1413608669513607E-4	0.0	0.0	22.86689675589182	0.0
25	2.1413608669513607E-4	0.0	0.0	23.64951061874087	0.0
26	2.1413608669513607E-4	0.0	0.0	24.35985355233031	0.0
27	2.1413608669513607E-4	0.0	0.0	25.097445302951705	0.0
28	2.1413608669513607E-4	0.0	0.0	25.87122605222458	0.0
29	2.1413608669513607E-4	0.0	0.0	26.708230481094194	0.0
30	2.1413608669513607E-4	0.0	0.0	27.582119850897044	0.0
31	2.1413608669513607E-4	0.0	0.0	28.379616171771403	0.0
32	2.1413608669513607E-4	0.0	0.0	29.14552741985823	0.0
33	2.1413608669513607E-4	0.0	0.0	29.913740630877033	0.0
34	2.1413608669513607E-4	0.0	0.0	30.688217322431665	0.0
35	2.1413608669513607E-4	0.0	0.0	31.52805905444999	0.0
36	2.1413608669513607E-4	0.0	0.0	32.306497263608485	0.0
37	2.1413608669513607E-4	0.0	0.0	33.114593320774254	0.0
38	2.1413608669513607E-4	0.0	0.0	33.973225494400076	0.0
39	2.1413608669513607E-4	0.0	0.0	35.06687202317381	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAT	1900	0.0	44.28947	1
CGTTTTT	3800	0.0	42.453945	1
CGTTATT	1370	0.0	41.715332	1
GTTTACG	125	0.0	41.4	1
GCGTTAG	120	0.0	41.249996	1
CCGTTAG	55	6.184564E-11	40.909092	1
ATGGGCG	730	0.0	40.68493	5
GCGTACG	50	1.0822987E-9	40.500004	1
TCTACGG	490	0.0	39.94898	2
TAGGGCG	1170	0.0	39.807693	5
AGGGCGA	1360	0.0	39.54044	6
CGTAGAC	40	3.4597724E-7	39.375	15
CCGTAGG	120	0.0	39.374996	2
TTATGGG	5460	0.0	39.27198	3
TAGGGCA	1430	0.0	39.020977	5
ACGGGAG	515	0.0	38.883495	5
CTAGGGT	1020	0.0	38.82353	4
TAGGGTA	1380	0.0	38.804348	5
TTAGGGA	3275	0.0	38.748093	4
CGCATGG	215	0.0	38.720932	2
>>END_MODULE