FastQCFastQC Report
Sat 14 Jan 2017
SRR2934139.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934139.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859776
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC129390.6957289480023401No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG114830.6174399497573901No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC100420.5399575002580955RNA PCR Primer, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46930.25234221755738323No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCAAAGCT41030.2206179668949379No Hit
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC36230.1948084070339654RNA PCR Primer, Index 13 (95% over 24bp)
GAATCAGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC35820.1926038404625073RNA PCR Primer, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC34970.1880333975704601Illumina Single End Adapter 1 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTC34830.18728061874118174No Hit
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT33280.17894628170274268RNA PCR Primer, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT30620.16464348394645376No Hit
GAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT24390.13114482604356653Illumina Single End Adapter 1 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21860.11754103720017894No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTA21660.11646563887263842No Hit
GAATATGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC21190.1139384528029182RNA PCR Primer, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG20980.11280928455900065RNA PCR Primer, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC20540.1104434082384115Illumina Single End Adapter 1 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT12400.043.911291
TCGTTAG2400.043.1249961
CGTTATT10750.041.8604621
CGTAAGG700.041.7857132
TTAGGGA37400.041.2098924
CTCGAAT1600.040.78125443
CCGATGA11700.039.99999618
TTTACGG9250.039.891892
TTATGGG47950.039.8852963
CGTTTTT30550.039.7708661
TAATACG3800.039.671054
TACGGGA9050.039.033154
CGTTAGG4500.039.02
TTTTACG6000.039.01
ATGGGCG6550.038.8167955
TTACGGG16500.038.590913
CGATGAA12150.038.51851719
TATGGGA31350.038.325364
TAGGGTA7050.038.2978745
TATGGGC13250.038.2075464
Produced by FastQC (version 0.11.5)