##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934139.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1859776
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.92916835145738	33.0	31.0	34.0	30.0	34.0
2	32.09786985099281	33.0	31.0	34.0	30.0	34.0
3	32.16792291114629	34.0	31.0	34.0	30.0	34.0
4	35.6966957310988	37.0	35.0	37.0	35.0	37.0
5	29.648299580164494	37.0	35.0	37.0	0.0	37.0
6	23.81873354640559	35.0	11.0	37.0	0.0	37.0
7	30.454138025224545	35.0	19.0	37.0	17.0	37.0
8	34.14122883616091	35.0	32.0	37.0	32.0	37.0
9	36.88864089008569	38.0	37.0	39.0	34.0	39.0
10	36.98139722203104	39.0	37.0	39.0	34.0	39.0
11	37.17087810575037	39.0	37.0	39.0	34.0	39.0
12	37.095043166488864	39.0	37.0	39.0	34.0	39.0
13	37.00273366254861	39.0	37.0	39.0	33.0	39.0
14	37.940681565952026	40.0	37.0	41.0	33.0	41.0
15	37.82201673749957	40.0	37.0	41.0	33.0	41.0
16	38.135002817543615	40.0	37.0	41.0	33.0	41.0
17	38.124041282391	40.0	37.0	41.0	33.0	41.0
18	38.205020927251454	40.0	37.0	41.0	34.0	41.0
19	38.18904480969751	40.0	37.0	41.0	34.0	41.0
20	38.05908883650504	40.0	37.0	41.0	33.0	41.0
21	37.987089843077875	40.0	37.0	41.0	33.0	41.0
22	38.10037284060016	40.0	37.0	41.0	34.0	41.0
23	38.001559865274096	40.0	36.0	41.0	34.0	41.0
24	37.98875563508724	40.0	36.0	41.0	34.0	41.0
25	37.78808899566399	40.0	36.0	41.0	33.0	41.0
26	37.697060828831	40.0	36.0	41.0	33.0	41.0
27	36.84528997040504	39.0	35.0	41.0	32.0	41.0
28	37.19688392580612	39.0	35.0	41.0	32.0	41.0
29	37.45443644826044	40.0	35.0	41.0	32.0	41.0
30	37.427427281565095	39.0	35.0	41.0	33.0	41.0
31	37.4258405313328	39.0	35.0	41.0	33.0	41.0
32	37.31564876630304	39.0	35.0	41.0	33.0	41.0
33	36.093571483877625	39.0	35.0	41.0	30.0	41.0
34	36.42963883822568	39.0	35.0	41.0	30.0	41.0
35	36.710188216215286	39.0	35.0	41.0	30.0	41.0
36	36.839020398155476	39.0	35.0	41.0	31.0	41.0
37	36.93598046216319	39.0	35.0	41.0	31.0	41.0
38	36.9044422554114	39.0	35.0	41.0	31.0	41.0
39	36.860030455280636	39.0	35.0	41.0	31.0	41.0
40	36.77260756134072	39.0	35.0	41.0	31.0	41.0
41	36.69054068877112	39.0	35.0	41.0	31.0	41.0
42	36.806126114112665	39.0	35.0	41.0	31.0	41.0
43	36.72417699765993	39.0	35.0	41.0	31.0	41.0
44	36.78767389190956	39.0	35.0	41.0	32.0	41.0
45	36.713173521972536	39.0	35.0	41.0	32.0	41.0
46	36.64764036098971	39.0	35.0	41.0	31.0	41.0
47	36.56279519684091	39.0	35.0	40.0	31.0	41.0
48	36.52868463728965	39.0	35.0	40.0	31.0	41.0
49	36.566411761416425	39.0	35.0	40.0	31.0	41.0
50	36.467235839154824	39.0	35.0	40.0	31.0	41.0
51	35.7842137977907	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	13.0
10	19.0
11	16.0
12	22.0
13	20.0
14	29.0
15	43.0
16	73.0
17	160.0
18	320.0
19	608.0
20	1256.0
21	2468.0
22	4305.0
23	6852.0
24	9522.0
25	12645.0
26	15123.0
27	16605.0
28	18740.0
29	22677.0
30	30727.0
31	42959.0
32	62824.0
33	99951.0
34	134926.0
35	159960.0
36	210030.0
37	314943.0
38	369344.0
39	322402.0
40	191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.608365738669605	4.04473441962903	52.79141143879693	16.555488402904437
2	20.95687867786228	8.905588630028562	49.31222899962146	20.825303692487697
3	19.346415912453974	8.891232062355897	51.065719742592655	20.696632282597474
4	17.268638803812934	4.421769073264737	53.820083709005814	24.489508413916514
5	17.264928679582916	21.689977717746654	40.957566932791906	20.08752666987852
6	15.632366478543652	31.66897518840979	39.362159743969166	13.336498589077394
7	79.70379228466223	1.8770540108056024	13.787090488316872	4.632063216215286
8	80.83919783887951	5.1183583399291095	9.207184090987301	4.835259730204068
9	75.69658926666438	4.616362400633194	10.702633005265149	8.984415327437283
10	39.69478044667745	25.724065693933035	19.002664837055647	15.578489022333871
11	32.377608916342616	22.296394834646755	25.42381448088372	19.902181768126916
12	26.543572989435287	20.15753510100141	30.321070924670497	22.977820984892805
13	25.887526239719193	20.780298272480128	34.195139629718845	19.137035858081834
14	21.360421900271863	24.05590780825218	33.648568429746376	20.935101861729585
15	18.84780747788981	22.045181785333288	37.248840720602914	21.858170016173993
16	22.859365859114217	23.146282132902027	30.30456356034275	23.689788447641007
17	23.240702106060084	24.614577239409478	29.434942702777107	22.70977795175333
18	24.196462369661724	21.99313250628033	30.82349702329743	22.986908100760523
19	23.707801369627308	25.202336195326748	28.170435579338587	22.919426855707353
20	23.786144137788636	27.1775740734368	29.827893251660413	19.208388537114146
21	23.02707422829416	27.715434546956192	30.36795829175126	18.88953293299838
22	21.62615282700712	22.219611394060358	32.49230014797481	23.661935630957707
23	21.253365996765204	25.07597689184074	31.103907137203617	22.56674997419044
24	22.074325080009636	25.023013524209368	29.388700574692866	23.51396082108813
25	22.250260246395264	27.757321311813897	26.17965819539557	23.812760246395264
26	20.454882738566365	23.82948269038852	29.300517911834547	26.415116659210568
27	20.6900185828831	25.470755617880865	29.312669912935753	24.526555886300287
28	17.613357737706046	24.757443907223237	30.709666110327266	26.919532244743454
29	20.35261235761726	24.286419439760486	29.872307202587837	25.488661000034412
30	22.3924816752125	24.818795381809423	29.643086049072576	23.145636893905504
31	22.142344024226574	24.786479662066828	27.693012491826973	25.378163821879625
32	23.924547902543104	26.43942066141299	26.406728552255753	23.229302883788154
33	22.089219346846072	27.73065143329089	24.27808510272205	25.90204411714099
34	20.167428765614783	25.95248029870264	29.609694931002444	24.270396004680133
35	20.686308458653084	26.96297833717609	27.09622018995836	25.254493014212464
36	21.15335395230393	27.087885852919925	25.973450566089678	25.785309628686466
37	19.243392752675593	28.444016913864896	26.35457173337004	25.95801860008947
38	21.282993220688944	27.686022402697958	25.050705031143533	25.980279345469565
39	21.000754929625934	28.042248098695755	25.478283406173645	25.478713565504663
40	23.011588492377577	25.985279947692625	27.55245793041743	23.450673629512373
41	20.499511769159295	25.551786881861045	26.006196445163287	27.942504903816374
42	21.899465311951545	22.664880071578512	28.9437007467566	26.491953869713342
43	20.68356619291786	22.702411473209676	28.842882153549677	27.77114018032279
44	20.99537793798823	23.137248786950686	28.37610550948071	27.491267765580368
45	20.666090970095325	22.497870711311467	27.126815272376888	29.70922304621632
46	22.295265666402837	24.406487663030386	27.380447967927317	25.917798702639455
47	17.855860060566435	24.947896951030664	32.04504198355071	25.151201004852197
48	18.99131938470009	26.32058914621976	28.2424872672838	26.445604201796346
49	21.572275370797346	22.942601689665853	30.22213427853677	25.262988661000037
50	20.395950910217145	23.6186508482742	28.2789970405038	27.706401201004855
51	19.36292327678172	23.109772359682026	27.054010805602395	30.473293557933857
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	351.0
1	952.5
2	1554.0
3	7719.0
4	13884.0
5	9288.0
6	4692.0
7	4542.0
8	4392.0
9	4545.5
10	4699.0
11	4737.0
12	4775.0
13	4717.5
14	4660.0
15	4491.5
16	4323.0
17	4319.5
18	4316.0
19	4455.5
20	4595.0
21	4995.0
22	5395.0
23	5805.5
24	6216.0
25	7939.5
26	11343.5
27	13024.0
28	14808.0
29	16592.0
30	20519.5
31	24447.0
32	29284.0
33	34121.0
34	39278.5
35	44436.0
36	48309.5
37	52183.0
38	56953.0
39	61723.0
40	69940.0
41	78157.0
42	89094.0
43	100031.0
44	115896.0
45	131761.0
46	175068.5
47	218376.0
48	211834.0
49	205292.0
50	195300.0
51	185308.0
52	165288.5
53	145269.0
54	129675.5
55	114082.0
56	104069.5
57	94057.0
58	84611.0
59	75165.0
60	66035.0
61	56905.0
62	50189.0
63	43473.0
64	38101.0
65	32729.0
66	27280.5
67	21832.0
68	18145.0
69	14458.0
70	11848.5
71	9239.0
72	7667.5
73	6096.0
74	4977.0
75	2833.0
76	1808.0
77	1407.0
78	1006.0
79	798.5
80	591.0
81	386.0
82	181.0
83	115.0
84	49.0
85	29.0
86	9.0
87	6.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1859776.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.60288243665027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.43297361879198	29.477603036353102
2	10.836134399362047	8.58284313371354
3	3.9243175016352363	4.662428539840481
4	2.0298638724805236	3.215538412169993
5	1.3154414876319307	2.6047637293489845
6	0.9437800107826213	2.2425845287850805
7	0.7464893599453791	2.069419125348901
8	0.6133934949928062	1.9433720375684902
9	0.5043687807484678	1.7977011775828384
>10	4.515059763349165	31.657207281820316
>50	0.08741413797428767	2.3248405738528053
>100	0.043279243470750184	2.954357583483338
>500	0.003579461616678428	0.9551844237732312
>1k	0.0034167588159203176	3.291468614084201
>5k	0.0	0.0
>10k+	4.881084022743311E-4	2.2206878022747327
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGC	12939	0.6957289480023401	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCG	11483	0.6174399497573901	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	10042	0.5399575002580955	RNA PCR Primer, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4693	0.25234221755738323	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCAAAGCT	4103	0.2206179668949379	No Hit
CCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	3623	0.1948084070339654	RNA PCR Primer, Index 13 (95% over 24bp)
GAATCAGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	3582	0.1926038404625073	RNA PCR Primer, Index 13 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	3497	0.1880333975704601	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTC	3483	0.18728061874118174	No Hit
CTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGCT	3328	0.17894628170274268	RNA PCR Primer, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCT	3062	0.16464348394645376	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCT	2439	0.13114482604356653	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2186	0.11754103720017894	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTA	2166	0.11646563887263842	No Hit
GAATATGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTC	2119	0.1139384528029182	RNA PCR Primer, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTG	2098	0.11280928455900065	RNA PCR Primer, Index 13 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCCAAAGCTCGTATGCCGTCTTCTGC	2054	0.1104434082384115	Illumina Single End Adapter 1 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19330284937540865	0.0
2	0.0	0.0	0.0	0.7399278192642554	0.0
3	0.0	0.0	0.0	1.069161017240786	0.0
4	0.0	0.0	0.0	1.5466916445851544	0.0
5	0.0	0.0	0.0	2.7443627619670328	0.0
6	0.0	0.0	0.0	3.564730376131319	0.0
7	0.0	0.0	0.0	4.577540521008982	0.0
8	0.0	0.0	0.0	6.026263377955194	0.0
9	0.0	0.0	0.0	6.6734918613854575	0.0
10	0.0	0.0	0.0	8.454297721876182	0.0
11	0.0	0.0	0.0	10.225586307168175	0.0
12	5.376991637702605E-5	0.0	0.0	12.601732681785334	0.0
13	1.075398327540521E-4	0.0	0.0	13.213956949654152	0.0
14	1.075398327540521E-4	0.0	0.0	13.478289858563612	0.0
15	1.075398327540521E-4	0.0	0.0	13.8979640558863	0.0
16	1.6130974913107816E-4	0.0	0.0	14.534546095873912	0.0
17	1.6130974913107816E-4	0.0	0.0	15.332868044323618	0.0
18	1.6130974913107816E-4	0.0	0.0	16.184529749819333	0.0
19	1.6130974913107816E-4	0.0	0.0	17.079691317664064	0.0
20	1.6130974913107816E-4	0.0	0.0	17.778807770398156	0.0
21	1.6130974913107816E-4	0.0	0.0	18.7586569565367	0.0
22	1.6130974913107816E-4	0.0	0.0	19.828785832272274	0.0
23	1.6130974913107816E-4	0.0	0.0	20.81589395712172	0.0
24	1.6130974913107816E-4	0.0	0.0	21.589051584706976	0.0
25	1.6130974913107816E-4	0.0	0.0	22.259885061426754	0.0
26	1.6130974913107816E-4	0.0	0.0	22.882379383323585	0.0
27	1.6130974913107816E-4	0.0	0.0	23.56498847172993	0.0
28	1.6130974913107816E-4	0.0	0.0	24.223777487181252	0.0
29	1.6130974913107816E-4	0.0	0.0	24.949026119274578	0.0
30	1.6130974913107816E-4	0.0	0.0	25.734927217041193	0.0
31	1.6130974913107816E-4	0.0	0.0	26.393124763412366	0.0
32	1.6130974913107816E-4	0.0	0.0	27.05320425685674	0.0
33	1.6130974913107816E-4	0.0	0.0	27.72000498984824	0.0
34	1.6130974913107816E-4	0.0	0.0	28.422724026979594	0.0
35	2.150796655081042E-4	0.0	0.0	29.195935424481227	0.0
36	2.150796655081042E-4	0.0	0.0	29.8871476995079	0.0
37	2.150796655081042E-4	0.0	0.0	30.603524295399016	0.0
38	2.150796655081042E-4	0.0	0.0	31.424967307890842	0.0
39	2.150796655081042E-4	0.0	0.0	32.74630923293988	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAT	1240	0.0	43.91129	1
TCGTTAG	240	0.0	43.124996	1
CGTTATT	1075	0.0	41.860462	1
CGTAAGG	70	0.0	41.785713	2
TTAGGGA	3740	0.0	41.209892	4
CTCGAAT	160	0.0	40.781254	43
CCGATGA	1170	0.0	39.999996	18
TTTACGG	925	0.0	39.89189	2
TTATGGG	4795	0.0	39.885296	3
CGTTTTT	3055	0.0	39.770866	1
TAATACG	380	0.0	39.67105	4
TACGGGA	905	0.0	39.03315	4
CGTTAGG	450	0.0	39.0	2
TTTTACG	600	0.0	39.0	1
ATGGGCG	655	0.0	38.816795	5
TTACGGG	1650	0.0	38.59091	3
CGATGAA	1215	0.0	38.518517	19
TATGGGA	3135	0.0	38.32536	4
TAGGGTA	705	0.0	38.297874	5
TATGGGC	1325	0.0	38.207546	4
>>END_MODULE