FastQCFastQC Report
Sat 14 Jan 2017
SRR2934138.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934138.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752634
Sequences flagged as poor quality0
Sequence length51
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC26660.1521139039867993TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT22680.12940522664743467TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC20580.11742326121711663No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC19080.10886471448117518TruSeq Adapter, Index 21 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG302.1661563E-645.0000049
GTCGATC453.8562575E-1045.030
TCGTTAG2100.041.7857131
AATACGG650.041.538465
TATGGGA35650.040.7713854
TAGGGTA4550.040.549455
TTAGGGA42900.040.541964
TCTATCG501.0822987E-940.52
TTATGGG49950.040.2252243
TAGGGAT25000.040.145
CTAGGGA23650.039.767444
TACGGGT2100.039.6428574
ATGGGCG6550.039.503825
TAGGGAG25650.039.4736865
CTACGGG8500.039.4411773
TACGGGA11650.039.3991434
TAGGGCG6750.039.3333325
TTACGGG15350.038.9902273
TGGGCGA17950.038.9832886
CGTTAGG4750.038.8421062
Produced by FastQC (version 0.11.5)