##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934138.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1752634
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04890638889808	33.0	31.0	34.0	30.0	34.0
2	32.27481265341195	34.0	31.0	34.0	30.0	34.0
3	32.34792090076993	34.0	31.0	34.0	30.0	34.0
4	35.75293871966423	37.0	35.0	37.0	35.0	37.0
5	29.219464531670617	37.0	35.0	37.0	0.0	37.0
6	23.33078611963479	35.0	0.0	37.0	0.0	37.0
7	30.263346483064918	35.0	19.0	37.0	17.0	37.0
8	34.307925670733304	35.0	32.0	37.0	32.0	37.0
9	36.976383546136844	38.0	37.0	39.0	34.0	39.0
10	37.08628156249394	39.0	37.0	39.0	34.0	39.0
11	37.23475922525753	39.0	37.0	39.0	34.0	39.0
12	37.107824565767864	39.0	37.0	39.0	34.0	39.0
13	36.98920481971707	39.0	37.0	39.0	33.0	39.0
14	37.77952327753541	40.0	37.0	41.0	33.0	41.0
15	37.778258894897625	40.0	37.0	41.0	33.0	41.0
16	38.11521800900815	40.0	37.0	41.0	33.0	41.0
17	38.16346995436583	40.0	37.0	41.0	33.0	41.0
18	38.276052501549096	40.0	37.0	41.0	34.0	41.0
19	38.270301158142544	40.0	37.0	41.0	34.0	41.0
20	38.178809722965546	40.0	37.0	41.0	33.0	41.0
21	38.08550216417118	40.0	37.0	41.0	33.0	41.0
22	38.238154686032566	40.0	37.0	41.0	34.0	41.0
23	38.21419589029997	40.0	37.0	41.0	34.0	41.0
24	38.1815758452706	40.0	37.0	41.0	34.0	41.0
25	37.953183037645054	40.0	36.0	41.0	34.0	41.0
26	37.81571166598388	40.0	36.0	41.0	34.0	41.0
27	36.93038249857072	40.0	35.0	41.0	33.0	41.0
28	37.29806051919568	40.0	35.0	41.0	33.0	41.0
29	37.602113732815866	40.0	35.0	41.0	33.0	41.0
30	37.647775861931244	40.0	36.0	41.0	33.0	41.0
31	37.643236408742496	40.0	36.0	41.0	33.0	41.0
32	37.653617355363416	40.0	36.0	41.0	33.0	41.0
33	36.41478882641784	40.0	35.0	41.0	31.0	41.0
34	36.87600719830837	40.0	35.0	41.0	31.0	41.0
35	37.179709511512385	40.0	35.0	41.0	31.0	41.0
36	37.32248604101027	39.0	35.0	41.0	32.0	41.0
37	37.42989123798808	40.0	35.0	41.0	33.0	41.0
38	37.348239849278286	39.0	35.0	41.0	33.0	41.0
39	37.383343584570426	39.0	35.0	41.0	33.0	41.0
40	37.28149687841272	39.0	35.0	41.0	33.0	41.0
41	37.12845237511083	39.0	35.0	41.0	32.0	41.0
42	37.203976414927475	39.0	35.0	41.0	33.0	41.0
43	37.13895599423496	39.0	35.0	41.0	33.0	41.0
44	37.17588897624946	39.0	35.0	41.0	33.0	41.0
45	37.073123652742105	39.0	35.0	41.0	33.0	41.0
46	37.00508092391224	39.0	35.0	41.0	33.0	41.0
47	36.93657660412841	39.0	35.0	41.0	32.0	41.0
48	36.88421028006989	39.0	35.0	40.0	32.0	41.0
49	36.90313322690305	39.0	35.0	40.0	32.0	41.0
50	36.811889989581395	39.0	35.0	40.0	32.0	41.0
51	36.012652384924635	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	11.0
11	22.0
12	17.0
13	38.0
14	38.0
15	68.0
16	77.0
17	159.0
18	268.0
19	477.0
20	841.0
21	1464.0
22	2337.0
23	3609.0
24	5277.0
25	7425.0
26	9920.0
27	12137.0
28	15782.0
29	20649.0
30	28352.0
31	40618.0
32	59851.0
33	98505.0
34	129474.0
35	145382.0
36	193506.0
37	297138.0
38	357495.0
39	321464.0
40	226.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.032645720669574	4.103594931970965	54.2744805817986	15.589278765560866
2	20.11235660154944	4.744858310405937	53.10053325451862	22.042251833526
3	22.01977138409959	5.1064283815103435	51.04591146811028	21.82788876627978
4	19.385507755755054	4.699041556879531	50.56783104744059	25.347619639924822
5	15.428435143903405	23.315021847116967	40.57544244833776	20.68110056064187
6	16.66274875416088	30.433678680203624	39.20727316713016	13.696299398505335
7	85.23981618523891	2.035222413806876	8.666726766683745	4.058234634270475
8	87.02706897161644	2.6168612499814565	6.307477773454127	4.048592004947981
9	81.66222953565891	5.02808915038736	8.207303977898409	5.102377336055332
10	40.795853555277375	30.763810356298006	14.86374223026599	13.576593858158633
11	32.52544455944595	22.779028593534075	25.576132837774455	19.119394009245514
12	28.516278926461542	21.428832260471953	29.91862533763467	20.136263475431836
13	27.542259250933164	21.76392789367318	31.45762321169166	19.23618964370199
14	21.45251090644139	23.341724512933105	35.340179409962374	19.865585170663127
15	20.17671687300372	22.306996212557785	35.96934670901055	21.546940205427944
16	26.26794869892973	23.406255955322102	29.309427980970355	21.016367364777814
17	25.886865141267375	22.919445816981753	28.66268713262438	22.53100190912649
18	27.610727624820697	20.60658414706094	29.980760386937604	21.80192784118076
19	26.26880455360332	23.812102241540448	28.075057313734643	21.844035891121592
20	25.15739167447396	28.69931771265421	27.583511446200404	18.559779166671422
21	24.645134123838748	25.560156883867368	30.65232102081781	19.142387971476076
22	23.182820828535792	21.12500385134603	33.06697233991809	22.625202980200086
23	22.042993574243113	23.956741681377856	31.882355357707315	22.11790938667172
24	21.736768772031127	26.94841022141531	28.87191507182903	22.442905934724536
25	24.87290558097127	26.325233905082296	25.720030536894754	23.081829977051683
26	22.216503845069763	25.220325521472255	26.507131551710167	26.056039081747816
27	21.876273083826973	25.502072880019444	30.105201656478194	22.516452379675393
28	18.87861356107436	26.535260641982294	28.787698971947368	25.79842682499598
29	21.42489532897342	26.944701517829735	28.132856032691368	23.49754712050548
30	25.32787792545392	23.714363637815996	29.25476739581681	21.70299104091328
31	24.589104171207453	25.598727401157344	28.319090009665455	21.493078417969752
32	25.683171728951965	25.64756817453045	26.649773997309197	22.01948609920839
33	24.538836973378356	26.271143889711148	25.141701005458074	24.048318131452433
34	21.36327379247464	25.217016216734358	30.359789893383333	23.059920097407673
35	20.937343449915954	28.774176468104578	27.38951772018573	22.898962361793735
36	24.98433785947323	27.85727082779405	24.518581746103294	22.639809566629427
37	23.33961340473824	27.969216619100166	25.875739030510648	22.815430945650945
38	22.57659043473994	27.13692647751898	27.115758338592084	23.170724749148995
39	21.96128798140399	22.811950469978328	27.259998379581816	27.966763169035865
40	25.075172568830684	22.879848274083464	27.933270722809212	24.11170843427664
41	21.858585420572695	25.37580578717519	25.740000479278617	27.025608312973503
42	21.07102794993136	25.138334643741935	29.170494238956906	24.620143167369797
43	21.114048911524026	23.627579973913548	29.52966791697525	25.728703197587176
44	22.920529899568308	23.387655380415993	28.625086583964475	25.066728136051225
45	22.31629650001084	22.45038039887392	26.41641095630919	28.816912144806047
46	22.57465049747979	23.560823309373205	28.070207470584275	25.794318722562725
47	19.11129191833549	26.550209570281076	31.197386333940802	23.14111217744264
48	20.304524504260442	28.165207339353227	27.9728682657075	23.55739989067883
49	23.279475349673692	24.19307168524632	29.363004483537352	23.16444848154264
50	21.50517449735655	24.76335618275122	26.809248251488903	26.922221068403328
51	20.363635533716682	24.589047114229214	25.76607551833412	29.28124183371999
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	223.0
1	933.0
2	1643.0
3	3536.5
4	5430.0
5	3860.5
6	2291.0
7	2195.0
8	2099.0
9	2277.0
10	2455.0
11	2460.5
12	2466.0
13	2433.0
14	2400.0
15	2392.5
16	2385.0
17	2414.5
18	2444.0
19	2863.5
20	3283.0
21	3720.5
22	4158.0
23	5575.0
24	6992.0
25	8471.5
26	11571.0
27	13191.0
28	16317.0
29	19443.0
30	23228.5
31	27014.0
32	31969.5
33	36925.0
34	41539.0
35	46153.0
36	50474.0
37	54795.0
38	59361.5
39	63928.0
40	70477.5
41	77027.0
42	86083.0
43	95139.0
44	106404.0
45	117669.0
46	136433.5
47	155198.0
48	170788.5
49	186379.0
50	182518.5
51	178658.0
52	161362.5
53	144067.0
54	132326.0
55	120585.0
56	110640.5
57	100696.0
58	89399.5
59	78103.0
60	66935.5
61	55768.0
62	49609.0
63	43450.0
64	37505.0
65	31560.0
66	26369.0
67	21178.0
68	17176.5
69	13175.0
70	11380.0
71	9585.0
72	7945.5
73	6306.0
74	5147.5
75	3332.0
76	2675.0
77	1934.0
78	1193.0
79	747.5
80	302.0
81	247.5
82	193.0
83	113.0
84	33.0
85	23.0
86	13.0
87	9.0
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	7.5
94	13.0
95	7.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1752634.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.144038595379406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97690388991079	24.234381457976486
2	11.053084243865293	7.547938700410331
3	4.231175741868798	4.33408283502604
4	2.183888246773027	2.9826705834325478
5	1.4770989760078659	2.5217062223003985
6	1.1390726387805683	2.333552408487863
7	0.9040582249902112	2.160773924857518
8	0.7428932732342524	2.029230127484644
9	0.6806581040025502	2.0916374915988762
>10	6.422188820699515	41.01546501078436
>50	0.12026445074321172	2.7600972347197974
>100	0.062484426783219124	3.7786322812151525
>500	0.004087756536047587	0.9693643616674711
>1k	0.002141205804596355	1.240467360038511
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	2666	0.1521139039867993	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGCT	2268	0.12940522664743467	TruSeq Adapter, Index 14 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGC	2058	0.11742326121711663	No Hit
GCTGTCTCTTATACACATCTGACGCAGATCGACTCGTATGCCGTCTTCTGC	1908	0.10886471448117518	TruSeq Adapter, Index 21 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.705697823960964E-5	0.0	0.0	0.1408736792735962	0.0
2	5.705697823960964E-5	0.0	0.0	0.5359361966046533	0.0
3	5.705697823960964E-5	0.0	0.0	0.749386352199033	0.0
4	1.1411395647921928E-4	0.0	0.0	1.0302207990943917	0.0
5	1.1411395647921928E-4	0.0	0.0	1.593487288275818	0.0
6	1.1411395647921928E-4	0.0	0.0	2.099354457348197	0.0
7	1.1411395647921928E-4	0.0	0.0	2.738050271762387	0.0
8	1.1411395647921928E-4	0.0	0.0	3.7218837475479765	0.0
9	1.1411395647921928E-4	0.0	0.0	4.233970127248472	0.0
10	1.1411395647921928E-4	0.0	0.0	5.18162947883015	0.0
11	1.1411395647921928E-4	0.0	0.0	6.775230881062447	0.0
12	1.1411395647921928E-4	0.0	0.0	8.350060537453912	0.0
13	1.1411395647921928E-4	0.0	0.0	8.812107947238271	0.0
14	1.1411395647921928E-4	0.0	0.0	9.006272844187663	0.0
15	1.1411395647921928E-4	0.0	0.0	9.261032252027519	0.0
16	1.1411395647921928E-4	0.0	0.0	9.782247748246354	0.0
17	1.1411395647921928E-4	0.0	0.0	10.529009479446366	0.0
18	1.1411395647921928E-4	0.0	0.0	11.322786160715815	0.0
19	1.1411395647921928E-4	0.0	0.0	11.910473036583793	0.0
20	1.1411395647921928E-4	0.0	0.0	12.550538218475735	0.0
21	1.1411395647921928E-4	0.0	0.0	13.419573054043228	0.0
22	1.7117093471882892E-4	0.0	0.0	14.38714529103053	0.0
23	1.7117093471882892E-4	0.0	0.0	15.252471423012448	0.0
24	2.2822791295843857E-4	0.0	0.0	15.933845857149867	0.0
25	2.2822791295843857E-4	0.0	0.0	16.511148362978236	0.0
26	2.2822791295843857E-4	0.0	0.0	17.045030508366263	0.0
27	2.2822791295843857E-4	0.0	0.0	17.681272872716153	0.0
28	2.2822791295843857E-4	0.0	0.0	18.271470255626674	0.0
29	2.2822791295843857E-4	0.0	0.0	18.98833413022913	0.0
30	2.852848911980482E-4	0.0	0.0	19.871918495247723	0.0
31	2.852848911980482E-4	0.0	0.0	20.483341074063382	0.0
32	2.852848911980482E-4	0.0	0.0	21.09721710294334	0.0
33	2.852848911980482E-4	0.0	0.0	21.706015060759977	0.0
34	3.4234186943765785E-4	0.0	0.0	22.41449155956121	0.0
35	3.4234186943765785E-4	0.0	0.0	23.240904832383716	0.0
36	3.4234186943765785E-4	0.0	0.0	23.898258278682256	0.0
37	3.4234186943765785E-4	0.0	0.0	24.5849960687742	0.0
38	3.4234186943765785E-4	0.0	0.0	25.488093920350742	0.0
39	3.4234186943765785E-4	0.0	0.0	27.375310532604068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	30	2.1661563E-6	45.000004	9
GTCGATC	45	3.8562575E-10	45.0	30
TCGTTAG	210	0.0	41.785713	1
AATACGG	65	0.0	41.53846	5
TATGGGA	3565	0.0	40.771385	4
TAGGGTA	455	0.0	40.54945	5
TTAGGGA	4290	0.0	40.54196	4
TCTATCG	50	1.0822987E-9	40.5	2
TTATGGG	4995	0.0	40.225224	3
TAGGGAT	2500	0.0	40.14	5
CTAGGGA	2365	0.0	39.76744	4
TACGGGT	210	0.0	39.642857	4
ATGGGCG	655	0.0	39.50382	5
TAGGGAG	2565	0.0	39.473686	5
CTACGGG	850	0.0	39.441177	3
TACGGGA	1165	0.0	39.399143	4
TAGGGCG	675	0.0	39.333332	5
TTACGGG	1535	0.0	38.990227	3
TGGGCGA	1795	0.0	38.983288	6
CGTTAGG	475	0.0	38.842106	2
>>END_MODULE