##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934137.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 617335 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.826374658815716 31.0 31.0 34.0 30.0 34.0 2 32.03615864967967 33.0 31.0 34.0 30.0 34.0 3 32.107029408667906 33.0 31.0 34.0 30.0 34.0 4 35.60046652141868 37.0 35.0 37.0 33.0 37.0 5 29.32499858261722 37.0 35.0 37.0 0.0 37.0 6 23.465036001522673 35.0 10.0 37.0 0.0 37.0 7 30.248929673516 35.0 19.0 37.0 17.0 37.0 8 34.10874970639928 35.0 32.0 37.0 32.0 37.0 9 36.703115812322324 37.0 35.0 39.0 33.0 39.0 10 36.8465662889679 38.0 37.0 39.0 33.0 39.0 11 37.09188852081933 39.0 37.0 39.0 34.0 39.0 12 37.10124162731742 39.0 37.0 39.0 34.0 39.0 13 37.02520349567091 39.0 37.0 39.0 33.0 39.0 14 38.108689771355905 40.0 37.0 41.0 33.0 41.0 15 37.94385382328881 40.0 37.0 41.0 33.0 41.0 16 38.173792187386105 40.0 37.0 41.0 34.0 41.0 17 38.176218746709644 40.0 37.0 41.0 34.0 41.0 18 38.256696931163795 40.0 37.0 41.0 34.0 41.0 19 38.285993828310396 40.0 37.0 41.0 34.0 41.0 20 38.24469534369508 40.0 37.0 41.0 34.0 41.0 21 38.11687009484316 40.0 37.0 41.0 34.0 41.0 22 38.1778418524788 40.0 37.0 41.0 34.0 41.0 23 38.12084848583022 40.0 37.0 41.0 34.0 41.0 24 38.094164432601424 40.0 37.0 41.0 34.0 41.0 25 37.86310836093855 40.0 36.0 41.0 33.0 41.0 26 37.756696121230775 40.0 36.0 41.0 33.0 41.0 27 36.90829452404286 39.0 36.0 41.0 32.0 41.0 28 37.22431742894862 39.0 36.0 41.0 32.0 41.0 29 37.56582244648368 39.0 36.0 41.0 32.0 41.0 30 37.60385204143617 39.0 36.0 41.0 33.0 41.0 31 37.568739825216454 39.0 36.0 41.0 33.0 41.0 32 37.60211068544631 39.0 36.0 41.0 33.0 41.0 33 36.39454753091919 39.0 35.0 41.0 31.0 41.0 34 36.90565576226846 39.0 35.0 41.0 31.0 41.0 35 37.247588424437296 39.0 35.0 41.0 31.0 41.0 36 37.350655640778506 39.0 35.0 41.0 33.0 41.0 37 37.38686450630533 39.0 36.0 41.0 33.0 41.0 38 37.289299974892074 39.0 35.0 40.0 33.0 41.0 39 37.36516964047073 39.0 35.0 41.0 33.0 41.0 40 37.34788081025699 39.0 35.0 41.0 33.0 41.0 41 37.24711866328655 39.0 35.0 41.0 33.0 41.0 42 37.25790697109349 39.0 35.0 41.0 33.0 41.0 43 37.08721520730236 39.0 35.0 40.0 32.0 41.0 44 37.04521370082694 39.0 35.0 40.0 32.0 41.0 45 37.01995836944285 39.0 35.0 40.0 32.0 41.0 46 36.92878906914398 39.0 35.0 40.0 32.0 41.0 47 36.85136595203576 39.0 35.0 40.0 31.0 41.0 48 36.80321705394964 39.0 35.0 40.0 31.0 41.0 49 36.829415147367314 39.0 35.0 40.0 32.0 41.0 50 36.731748564393726 39.0 35.0 40.0 31.0 41.0 51 35.84598475706059 38.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 8.0 11 5.0 12 6.0 13 6.0 14 9.0 15 12.0 16 23.0 17 41.0 18 69.0 19 128.0 20 227.0 21 394.0 22 675.0 23 1037.0 24 1565.0 25 2300.0 26 3281.0 27 4051.0 28 5434.0 29 7260.0 30 9977.0 31 14351.0 32 20559.0 33 32644.0 34 45021.0 35 58127.0 36 76405.0 37 113155.0 38 124963.0 39 95553.0 40 45.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 23.984708464609977 5.048150517952165 52.61859444223963 18.34854657519823 2 19.70518438125167 4.95484623421643 51.58204216511294 23.757927219418953 3 21.473754120534235 5.11100132019082 50.79899892278908 22.616245636485864 4 21.028452946941286 4.969101055342723 48.6617476734674 25.340698324248585 5 15.051957203139303 22.83816728356565 40.365765751172376 21.744109762122672 6 16.737751787927138 29.699271870216332 38.90189281346433 14.661083528392203 7 79.40631909741064 2.437898385803494 11.713089327512614 6.442693189273246 8 78.6357488235723 3.2831444839511774 9.961204208411964 8.119902484064568 9 71.72167461750914 6.46002575586999 13.728688637449682 8.089610989171197 10 35.26189184154471 28.627244526877625 20.11371459580293 15.997149035774742 11 28.0938226408676 24.31418921655179 26.501332339815498 21.090655802765113 12 25.412134416483756 20.039200758097305 32.72566758729053 21.822997238128405 13 22.20204589080483 24.4789295925227 32.50423190002187 20.814792616650603 14 18.705079089959263 26.24361165331627 31.99203025909757 23.059278997626897 15 16.737103841512308 24.825904897664962 36.765613483764895 21.671377777057838 16 18.798545360298704 24.09728915418695 32.24894101257826 24.855224472936086 17 18.609506993771614 23.416297472199048 29.56757676140183 28.4066187726275 18 19.128998031862764 22.215328792308874 33.78457401572889 24.87109916009946 19 21.91273781658257 23.615379008156026 30.411526966719855 24.060356208541553 20 23.690864765483894 24.838053892943055 30.261203398478948 21.209877943094106 21 23.04534814970802 26.248633238031218 29.5730843059279 21.132934306332864 22 21.078344820883313 24.22234281224943 28.096900386338046 26.60241198052921 23 20.66090534312812 25.001984335895422 28.095604493508386 26.24150582746807 24 21.944001231098188 22.307337183214948 29.203106903059115 26.54555468262775 25 19.668899382021106 24.96472741704261 27.16547741501778 28.200895785918505 26 19.08283184980602 25.157653462058686 27.84322936493152 27.91628532320377 27 21.430179724136813 27.24744263649396 27.593769995221397 23.72860764414783 28 17.44498529971571 26.245069532749643 30.626321203236493 25.683623964298153 29 18.512639004754305 22.94605036163509 31.777559995788344 26.763750637822252 30 23.256578680943086 23.163598370414768 30.390792681445244 23.1890302671969 31 24.595883920399782 26.517692986789992 25.719746976925006 23.166676115885217 32 23.584925526658946 26.286700089902563 25.969206346635133 24.159168036803354 33 22.618837422145187 27.645605708407917 26.235836296338295 23.499720573108604 34 22.139681048377298 23.981792705743235 28.413584196586942 25.46494204929252 35 22.130609798569658 21.879530562822456 28.038585209003212 27.951274429604673 36 24.7957753893753 25.296475981436334 26.31456178574032 23.59318684344805 37 23.962030340090877 26.53745535244235 28.850786039994492 20.64972826747228 38 20.79697409024274 28.383454688297277 28.257105137405137 22.56246608405485 39 22.577854811407096 23.22709711906825 31.869892359901836 22.325155709622816 40 25.786161484445238 20.85836701304802 30.13355795475714 23.2219135477496 41 23.645184543238273 22.736763669644517 25.692533227502086 27.92551855961512 42 24.063919913823124 23.83827257485806 25.83378554593535 26.26402196538346 43 24.269156940721 23.845561972024914 27.439720735095207 24.445560352158875 44 20.645354629172168 22.660305992694404 29.315363619428673 27.378975758704755 45 22.4626823361708 20.031749374326743 28.362720403022667 29.14284788647979 46 24.381575643694266 20.968679890173085 30.30283395563187 24.346910510500784 47 20.457288182267327 22.691407420606318 32.951476912859306 23.89982748426705 48 20.667384807276438 22.19589039986393 30.953858115933812 26.18286667692582 49 22.239788769468767 20.109502944106524 34.21027481027319 23.440433476151522 50 23.60484987891501 19.20918140069816 30.56557622684604 26.620392493540784 51 21.139575757084888 19.163339191848834 27.910777778677705 31.786307272388576 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 123.0 1 323.5 2 524.0 3 1012.0 4 1500.0 5 1058.0 6 616.0 7 717.5 8 819.0 9 903.0 10 987.0 11 1006.5 12 1026.0 13 1034.0 14 1042.0 15 1053.0 16 1064.0 17 1000.0 18 936.0 19 1004.0 20 1072.0 21 1230.0 22 1388.0 23 1631.0 24 1874.0 25 2547.5 26 3567.0 27 3913.0 28 4543.0 29 5173.0 30 6025.0 31 6877.0 32 7718.5 33 8560.0 34 10097.0 35 11634.0 36 13038.0 37 14442.0 38 16340.0 39 18238.0 40 21475.0 41 24712.0 42 29518.5 43 34325.0 44 41540.0 45 48755.0 46 59776.5 47 70798.0 48 80204.5 49 89611.0 50 84569.0 51 79527.0 52 65528.0 53 51529.0 54 42987.5 55 34446.0 56 29671.0 57 24896.0 58 22866.5 59 20837.0 60 18787.5 61 16738.0 62 14792.0 63 12846.0 64 10965.0 65 9084.0 66 7095.0 67 5106.0 68 4276.5 69 3447.0 70 2849.0 71 2251.0 72 1807.0 73 1363.0 74 1201.0 75 753.0 76 467.0 77 379.0 78 291.0 79 213.0 80 135.0 81 82.5 82 30.0 83 26.5 84 23.0 85 15.0 86 7.0 87 5.0 88 3.0 89 10.5 90 18.0 91 19.0 92 20.0 93 11.0 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 617335.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.82251002192387 #Duplication Level Percentage of deduplicated Percentage of total 1 71.0517839227063 29.005121612602476 2 12.30561635920249 10.046922942989877 3 4.810184590330574 5.890914259382208 4 2.6485559428426684 4.324828060812834 5 1.6658041409704833 3.4001153119664917 6 1.2010937671882513 2.941899740898761 7 0.9515114714973004 2.7190160606821854 8 0.7341328672258396 2.397531706380043 9 0.589563397623811 2.166071193725176 >10 3.892890005890103 27.127711688787098 >50 0.09436158480653632 2.649298985097866 >100 0.04758752325918018 3.4696917014993702 >500 0.00406730968027181 1.1551249063181854 >1k 0.002847116776190267 2.7057518288574314 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC 4533 0.7342852746077899 No Hit CTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT 3175 0.514307466772498 Illumina Single End Adapter 2 (95% over 21bp) TCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC 2499 0.40480452266597555 No Hit GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC 2189 0.3545886755165348 No Hit CCTGTATCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC 1667 0.2700316683810249 No Hit CTGTCACTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGCT 1173 0.19001028614933546 Illumina Single End Adapter 2 (95% over 21bp) TCTGTATCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC 1060 0.17170579993034576 No Hit TCCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG 996 0.16133865729304186 No Hit GCTGTATCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC 938 0.1519434342779852 No Hit CCTGACTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC 865 0.14011841220731047 No Hit CGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTG 692 0.11209472976584836 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.555128090906882 0.0 2 0.0 0.0 0.0 2.205285622878988 0.0 3 0.0 0.0 0.0 2.961277102383633 0.0 4 0.0 0.0 0.0 3.744482331311201 0.0 5 0.0 0.0 0.0 5.068722816623065 0.0 6 0.0 0.0 0.0 6.019584180388282 0.0 7 0.0 0.0 0.0 7.244850850834636 0.0 8 0.0 0.0 0.0 9.227080920407882 0.0 9 0.0 0.0 0.0 10.194302931147593 0.0 10 0.0 0.0 0.0 11.9473219564742 0.0 11 0.0 0.0 0.0 14.917670308665473 0.0 12 0.0 0.0 0.0 17.748710181667978 0.0 13 0.0 0.0 0.0 18.682562952043867 0.0 14 0.0 0.0 0.0 19.012529663796805 0.0 15 0.0 0.0 0.0 19.52845699660638 0.0 16 0.0 0.0 0.0 20.68082969538419 0.0 17 0.0 0.0 0.0 22.296160107559103 0.0 18 0.0 0.0 0.0 24.15268857265504 0.0 19 0.0 0.0 0.0 25.339240444815214 0.0 20 0.0 0.0 0.0 26.611807203544267 0.0 21 0.0 0.0 0.0 28.183239246114347 0.0 22 0.0 0.0 0.0 29.642090599107455 0.0 23 0.0 0.0 0.0 31.047000413065838 0.0 24 0.0 0.0 0.0 32.128908939230726 0.0 25 0.0 0.0 0.0 33.024532871131555 0.0 26 0.0 0.0 0.0 33.84693885815643 0.0 27 0.0 0.0 0.0 34.59094332898669 0.0 28 0.0 0.0 0.0 35.405249985826174 0.0 29 0.0 0.0 0.0 36.31658661828667 0.0 30 0.0 0.0 0.0 37.296281597511886 0.0 31 1.6198660370787336E-4 0.0 0.0 38.20956206921687 0.0 32 1.6198660370787336E-4 0.0 0.0 39.03553176152332 0.0 33 3.239732074157467E-4 0.0 0.0 39.82618837422145 0.0 34 3.239732074157467E-4 0.0 0.0 40.68909101217329 0.0 35 3.239732074157467E-4 0.0 0.0 41.569326216721876 0.0 36 3.239732074157467E-4 0.0 0.0 42.41003668996574 0.0 37 3.239732074157467E-4 0.0 0.0 43.183036762859714 0.0 38 4.859598111236201E-4 0.0 0.0 43.930118979160426 0.0 39 4.859598111236201E-4 0.0 0.0 44.724987243554956 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTGAAT 25 3.8886992E-5 45.000004 21 CGTTGAT 35 1.2107012E-7 45.000004 25 ATAGCCG 25 3.8886992E-5 45.000004 31 GTCTACG 25 3.8886992E-5 45.000004 1 CGTTTAT 115 0.0 45.000004 1 CTCCCGT 20 7.030747E-4 45.0 25 GATCCAC 20 7.030747E-4 45.0 9 GAATCGG 20 7.030747E-4 45.0 21 CGCGCAA 20 7.030747E-4 45.0 16 CGTTACC 20 7.030747E-4 45.0 13 GTTCCGA 20 7.030747E-4 45.0 15 TCCAATC 20 7.030747E-4 45.0 28 TGAATCG 40 6.8066583E-9 45.0 31 CACCGGT 20 7.030747E-4 45.0 10 CGTCCGA 20 7.030747E-4 45.0 40 TCGACGT 45 3.8380676E-10 45.0 26 TAATGCG 20 7.030747E-4 45.0 24 CGATAAC 40 6.8066583E-9 45.0 16 AATCGAT 20 7.030747E-4 45.0 39 TGTCCGA 20 7.030747E-4 45.0 26 >>END_MODULE