FastQCFastQC Report
Sat 14 Jan 2017
SRR2934136.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934136.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847239
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC36540.19780872967710186TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT31110.1684135079434767TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC23190.12553870939277484TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC22990.1244560124596763TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACCG207.0343993E-445.00000444
TTAGGGA51300.041.7543874
TCGTTAG3050.041.3114781
TATGGGA41500.040.8795174
TTATGGG54300.040.773483
TAGGGAT30250.040.314055
ATCGTCG451.929402E-840.010
TAGGGAC19400.039.8969085
TTCGTAG2200.039.8863641
CGCGAGG1750.039.8571432
CGTTAGG5650.039.8230062
TACGAAT2150.039.7674412
CTAGGGT4600.039.6195644
TTTATGG31450.039.4912572
CGGTCTA403.459736E-739.37500431
GCTACGA2250.039.010
TAGGGAG25450.038.8113945
TGGGCGA18300.038.7295076
CTAGGGA28700.038.571434
CGATGTA356.2503514E-638.57142610
Produced by FastQC (version 0.11.5)