##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934136.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1847239
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.042792513583784	33.0	31.0	34.0	30.0	34.0
2	32.26743696944467	34.0	31.0	34.0	30.0	34.0
3	32.3470476749354	34.0	31.0	34.0	30.0	34.0
4	35.7357889260675	37.0	35.0	37.0	35.0	37.0
5	29.53876406896996	37.0	35.0	37.0	0.0	37.0
6	23.6806065701298	35.0	10.0	37.0	0.0	37.0
7	30.410880238020095	35.0	19.0	37.0	17.0	37.0
8	34.36944867448121	35.0	32.0	37.0	32.0	37.0
9	37.00037677853272	38.0	37.0	39.0	34.0	39.0
10	37.03941882994025	39.0	37.0	39.0	34.0	39.0
11	37.2097779442725	39.0	37.0	39.0	34.0	39.0
12	37.147117400617894	39.0	37.0	39.0	34.0	39.0
13	37.05376618834921	39.0	37.0	39.0	33.0	39.0
14	37.91419680940041	40.0	37.0	41.0	33.0	41.0
15	37.85030848742366	40.0	37.0	41.0	33.0	41.0
16	38.15401363873327	40.0	37.0	41.0	33.0	41.0
17	38.20907635665986	40.0	37.0	41.0	33.0	41.0
18	38.29742226100683	40.0	37.0	41.0	34.0	41.0
19	38.310326384403965	40.0	37.0	41.0	34.0	41.0
20	38.24739245977375	40.0	37.0	41.0	34.0	41.0
21	38.121060133529014	40.0	37.0	41.0	34.0	41.0
22	38.25480135488694	40.0	37.0	41.0	34.0	41.0
23	38.229634606025535	40.0	37.0	41.0	34.0	41.0
24	38.20386858441165	40.0	37.0	41.0	34.0	41.0
25	37.970526282738724	40.0	36.0	41.0	34.0	41.0
26	37.88730857241537	40.0	36.0	41.0	34.0	41.0
27	37.04616240778805	40.0	36.0	41.0	33.0	41.0
28	37.36450453893622	40.0	36.0	41.0	33.0	41.0
29	37.625934705796055	40.0	36.0	41.0	33.0	41.0
30	37.65945121340552	40.0	36.0	41.0	33.0	41.0
31	37.66337815518187	40.0	36.0	41.0	33.0	41.0
32	37.704121123471296	40.0	36.0	41.0	33.0	41.0
33	36.51667542748935	40.0	35.0	41.0	31.0	41.0
34	36.94471153976286	40.0	35.0	41.0	31.0	41.0
35	37.24968182243879	40.0	35.0	41.0	31.0	41.0
36	37.38904169953103	40.0	35.0	41.0	32.0	41.0
37	37.46329846868759	40.0	35.0	41.0	33.0	41.0
38	37.38026156875207	39.0	35.0	41.0	33.0	41.0
39	37.44013254375855	40.0	35.0	41.0	33.0	41.0
40	37.42581495951526	40.0	35.0	41.0	33.0	41.0
41	37.31871024810542	40.0	35.0	41.0	33.0	41.0
42	37.370911398037826	40.0	35.0	41.0	33.0	41.0
43	37.246639985405245	39.0	35.0	41.0	33.0	41.0
44	37.27637571532433	39.0	35.0	41.0	33.0	41.0
45	37.194011711532724	39.0	35.0	41.0	33.0	41.0
46	37.14996705894581	39.0	35.0	41.0	33.0	41.0
47	37.08395015479859	39.0	35.0	41.0	32.0	41.0
48	37.01110359839739	39.0	35.0	41.0	32.0	41.0
49	37.01758299819352	39.0	35.0	41.0	32.0	41.0
50	36.93363771553113	39.0	35.0	41.0	32.0	41.0
51	36.16927208661142	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	21.0
10	42.0
11	32.0
12	29.0
13	29.0
14	34.0
15	52.0
16	88.0
17	143.0
18	267.0
19	424.0
20	748.0
21	1280.0
22	2112.0
23	3345.0
24	5108.0
25	7408.0
26	10163.0
27	12885.0
28	16196.0
29	21329.0
30	28979.0
31	41408.0
32	60186.0
33	98832.0
34	132493.0
35	151301.0
36	201885.0
37	314072.0
38	383830.0
39	352273.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.040852320679672	4.1639982698503015	55.293603047575324	16.501546361894697
2	19.921623569012997	4.093568834352242	53.23046990670942	22.754337689925343
3	21.779423236516767	4.1433728932747735	52.34081783678235	21.736386033426104
4	19.29555406744877	4.731872811260481	50.58798563694249	25.384587484348266
5	15.2895754149842	22.21910646104808	41.570148746318154	20.921169377649562
6	16.628871521227083	29.286573096388718	40.05058360071436	14.033971781669832
7	85.41953694134868	2.0046133716319328	8.31841467184268	4.257435015176704
8	87.48743394872022	2.039422078031051	6.302811926339796	4.170332046908928
9	82.09235513109023	5.041036920506768	8.286908191089513	4.579699757313483
10	38.65195570253768	33.57935816643109	14.708058892216979	13.060627238814252
11	30.217746593700113	23.61194193063269	26.70910477745435	19.461206698212845
12	27.627448316108527	21.186213586872082	30.072665204664904	21.113672892354483
13	26.988657125580396	21.927536176964647	31.72496899426658	19.35883770318838
14	21.414337830675944	23.649024300591314	34.1294223432918	20.80721552544094
15	19.710389397365475	22.989661868334306	35.6298778880264	21.670070846273816
16	24.979604696522756	24.41378727928546	29.678617655863697	20.927990368328082
17	25.409272974422908	23.141726652587998	28.528847647759708	22.920152725229386
18	26.778289111479346	21.58572875518544	30.011492827944842	21.62448930539037
19	25.747182687242958	24.00247071440133	28.506977169711124	21.743369428644588
20	24.782012506232274	28.10139889857241	27.980245111758684	19.136343483436633
21	24.536727516038802	25.755032240007925	30.918522183648133	18.789718060305134
22	22.976452965750507	22.423140698090503	32.03440377774614	22.566002558412855
23	21.591575318624173	24.466839429007294	30.864982820306412	23.07660243206212
24	21.742936349871346	26.109777890137657	29.414168929954382	22.73311683003661
25	24.0394989495133	26.167648041211777	26.086554040922692	23.706298968352225
26	22.16026188273418	25.183097585098622	26.7348729644621	25.9217675677051
27	21.350512846469787	25.91662475727288	29.887199220025128	22.845663176232204
28	19.21391871869314	25.40959778350284	29.931319120048894	25.445164377755127
29	21.85131431287451	25.364178647159356	29.051465457366373	23.73304158259976
30	25.88674232191936	23.24517834454556	29.372809907110014	21.49526942642506
31	24.177975887256604	25.12641840065092	29.11317918255299	21.582426529539493
32	25.295102582827667	25.312371598910588	27.273839497758544	22.118686320503194
33	24.82602413656273	26.404975208946972	26.168081119985015	22.60091953450528
34	22.10569395730601	24.40442195081416	30.859136256867682	22.630747835012144
35	22.30534327176938	26.804598646953643	28.47682406012433	22.41323402115265
36	25.885659624986264	25.966861894968652	26.750842744225302	21.396635735819782
37	22.6730812850963	27.132709952529154	27.843717028494957	22.35049173387959
38	22.017778966338412	27.388009889353786	28.395892464375212	22.19831867993259
39	22.61125929021637	24.627295114492494	27.32575481570062	25.435690779590516
40	25.078400791668	23.080608410714586	28.023228180002697	23.817762617614722
41	21.36123154610746	23.641878500832863	28.194023621198987	26.802866331860685
42	22.032936723401793	24.02212166373707	30.314052485899225	23.630889126961915
43	21.409628099016963	24.459368820168912	29.88806537757161	24.242937703242514
44	23.156938544498033	23.250591829211054	28.529984479539465	25.062485146751452
45	23.191151767583946	22.217753089881707	26.89105199706156	27.700043145472787
46	23.38414249590876	22.32618518773153	28.840068881178883	25.44960343518083
47	20.057177225036934	25.00542701837716	31.48937414162434	23.448021614961572
48	20.578062719550637	26.710133339540796	29.787591102180066	22.924212838728504
49	23.902321248089716	23.54259519206773	29.792300833839043	22.76278272600351
50	21.962020074283835	23.794484633553104	28.269325192895995	25.97417009926707
51	20.938817337659067	23.243662568839223	26.833885598993955	28.983634494507747
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	331.0
1	1005.0
2	1679.0
3	3792.5
4	5906.0
5	4181.0
6	2456.0
7	2385.5
8	2315.0
9	2359.5
10	2404.0
11	2397.5
12	2391.0
13	2336.5
14	2282.0
15	2318.5
16	2355.0
17	2427.0
18	2499.0
19	2979.5
20	3460.0
21	3720.5
22	3981.0
23	5518.5
24	7056.0
25	9255.5
26	14269.5
27	17084.0
28	20308.0
29	23532.0
30	26871.0
31	30210.0
32	34665.0
33	39120.0
34	44726.0
35	50332.0
36	54313.0
37	58294.0
38	63590.5
39	68887.0
40	76171.5
41	83456.0
42	93480.5
43	103505.0
44	115193.0
45	126881.0
46	142688.5
47	158496.0
48	178225.5
49	197955.0
50	193219.5
51	188484.0
52	170117.5
53	151751.0
54	137921.5
55	124092.0
56	112765.5
57	101439.0
58	91677.5
59	81916.0
60	70289.0
61	58662.0
62	51676.5
63	44691.0
64	38593.5
65	32496.0
66	27436.5
67	22377.0
68	17388.0
69	12399.0
70	10020.5
71	7642.0
72	6318.0
73	4994.0
74	4443.5
75	3140.5
76	2388.0
77	1712.0
78	1036.0
79	702.5
80	369.0
81	267.0
82	165.0
83	122.5
84	80.0
85	58.5
86	37.0
87	21.5
88	6.0
89	3.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1847239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.954285503808656
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.62570396789869	25.752510098731097
2	11.525001705318678	8.287464034898187
3	4.334892357375366	4.675738723360561
4	2.1419432115092465	3.08048151038193
5	1.37248660484846	2.467338762043727
6	1.0073302722506738	2.1730704123078013
7	0.7592955151729929	1.9109949414003882
8	0.6493598147391573	1.8677814539065565
9	0.5764874419965326	1.865447467101329
>10	5.838454150363648	39.8262556918761
>50	0.11162577834909794	2.706493183000668
>100	0.052543987952563834	3.3991569984758483
>500	0.0034306908250089103	0.9004406730029706
>1k	0.0014445014000037519	1.0868260495128141
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	3654	0.19780872967710186	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	3111	0.1684135079434767	TruSeq Adapter, Index 16 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	2319	0.12553870939277484	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	2299	0.1244560124596763	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.413484665492662E-5	0.0	0.0	0.1787532636545677	0.0
2	5.413484665492662E-5	0.0	0.0	0.6667247714020763	0.0
3	5.413484665492662E-5	0.0	0.0	0.9589446736453702	0.0
4	5.413484665492662E-5	0.0	0.0	1.2855402035145425	0.0
5	5.413484665492662E-5	0.0	0.0	1.8592071735167999	0.0
6	5.413484665492662E-5	0.0	0.0	2.385776826929271	0.0
7	5.413484665492662E-5	0.0	0.0	3.036098739794905	0.0
8	5.413484665492662E-5	0.0	0.0	4.058272914333229	0.0
9	5.413484665492662E-5	0.0	0.0	4.541426420728449	0.0
10	5.413484665492662E-5	0.0	0.0	5.413863609419247	0.0
11	5.413484665492662E-5	0.0	0.0	7.168373989505419	0.0
12	5.413484665492662E-5	0.0	0.0	8.795992289032442	0.0
13	5.413484665492662E-5	0.0	0.0	9.370850225661108	0.0
14	5.413484665492662E-5	0.0	0.0	9.583546038168315	0.0
15	5.413484665492662E-5	0.0	0.0	9.854599215369532	0.0
16	5.413484665492662E-5	0.0	0.0	10.453005810293092	0.0
17	5.413484665492662E-5	0.0	0.0	11.27358181588847	0.0
18	5.413484665492662E-5	0.0	0.0	12.15527606335726	0.0
19	5.413484665492662E-5	0.0	0.0	12.80733029131585	0.0
20	5.413484665492662E-5	0.0	0.0	13.548977690488345	0.0
21	1.0826969330985324E-4	0.0	0.0	14.543055879612762	0.0
22	1.0826969330985324E-4	0.0	0.0	15.635388815415872	0.0
23	1.0826969330985324E-4	0.0	0.0	16.63488048920578	0.0
24	1.6240453996477986E-4	0.0	0.0	17.384215036603276	0.0
25	1.6240453996477986E-4	0.0	0.0	18.040708321987573	0.0
26	1.6240453996477986E-4	0.0	0.0	18.63933145629775	0.0
27	1.6240453996477986E-4	0.0	0.0	19.327114683048592	0.0
28	2.1653938661970649E-4	0.0	0.0	19.977761404994155	0.0
29	2.1653938661970649E-4	0.0	0.0	20.735162044543234	0.0
30	2.1653938661970649E-4	0.0	0.0	21.629036632509383	0.0
31	2.1653938661970649E-4	0.0	0.0	22.28433895126727	0.0
32	2.1653938661970649E-4	0.0	0.0	22.950522374202798	0.0
33	2.1653938661970649E-4	0.0	0.0	23.60533747934079	0.0
34	2.1653938661970649E-4	0.0	0.0	24.371832773127895	0.0
35	2.1653938661970649E-4	0.0	0.0	25.1910554075569	0.0
36	2.1653938661970649E-4	0.0	0.0	25.8712056209294	0.0
37	2.706742332746331E-4	0.0	0.0	26.593743419232702	0.0
38	2.706742332746331E-4	0.0	0.0	27.473164003141985	0.0
39	2.706742332746331E-4	0.0	0.0	29.08134789271989	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCAACCG	20	7.0343993E-4	45.000004	44
TTAGGGA	5130	0.0	41.754387	4
TCGTTAG	305	0.0	41.311478	1
TATGGGA	4150	0.0	40.879517	4
TTATGGG	5430	0.0	40.77348	3
TAGGGAT	3025	0.0	40.31405	5
ATCGTCG	45	1.929402E-8	40.0	10
TAGGGAC	1940	0.0	39.896908	5
TTCGTAG	220	0.0	39.886364	1
CGCGAGG	175	0.0	39.857143	2
CGTTAGG	565	0.0	39.823006	2
TACGAAT	215	0.0	39.76744	12
CTAGGGT	460	0.0	39.619564	4
TTTATGG	3145	0.0	39.491257	2
CGGTCTA	40	3.459736E-7	39.375004	31
GCTACGA	225	0.0	39.0	10
TAGGGAG	2545	0.0	38.811394	5
TGGGCGA	1830	0.0	38.729507	6
CTAGGGA	2870	0.0	38.57143	4
CGATGTA	35	6.2503514E-6	38.571426	10
>>END_MODULE