FastQCFastQC Report
Sat 14 Jan 2017
SRR2934135.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934135.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128016
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC87160.40958338659107824TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGCT86200.40507214231471944TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC66690.31339050082330205TruSeq Adapter, Index 22 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56220.2641897429342636No Hit
GCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTGC51230.2407406711227735TruSeq Adapter, Index 22 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTG39910.18754558236404237No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCC35270.16574123502830806No Hit
CGTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTC30000.14097638363621326No Hit
TCCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTTCTG27880.13101405252592085No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCTT23260.10930368944594403No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCATGGTTGTCGTATGCCGTCT21990.10333568920534433No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGG1400.045.0000042
GCGAGTC302.1663764E-645.0000049
CGTATCG207.034643E-445.02
CCCGTAG207.034643E-445.01
TAACCCG207.034643E-445.037
ACCCGTT253.8919297E-545.011
TCGCGCG406.8193913E-945.01
CGTTAGG5750.043.4347842
CGTTTAT16900.042.8698231
CGCTAGG3150.042.8571432
CTCGTAG1050.042.857141
TCCGTAG1050.042.857141
TCGTTAG3100.042.822581
CGTTTTT37600.042.7260631
CGTTATT15300.042.51
CAACGAG850.042.35294314
TCGCTAG1500.042.0000041
CACGGGA8750.041.6571434
TAGGGCG8650.041.6184965
ATGGGCG8350.041.4970055
Produced by FastQC (version 0.11.5)