FastQCFastQC Report
Sat 14 Jan 2017
SRR2934134.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934134.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1190828
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGC164411.38063599445092No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCG138221.1607049884618097No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC101240.8501647593103286No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTGGACT52860.44389282079359904No Hit
GCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC41050.3447181288985479TruSeq Adapter, Index 14 (95% over 21bp)
GAATCAGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC38770.3255717870254982No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCT34290.28795090474862867No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTC33930.2849277981370945No Hit
CCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC31420.26385002704000915TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGCT26920.22606119439583214TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTA25520.21430466868431042No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCT21870.18365372665070018No Hit
GAATATGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTC20890.17542415865263497No Hit
TCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC19770.16601893808341758TruSeq Adapter, Index 14 (95% over 21bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGC19130.16064452632957907No Hit
GCTGTATCTTATACACATCTGACGCCTTTGGACTCGTATGCCGTCTTCTGC16720.14040650706903096TruSeq Adapter, Index 21 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCCTTTGGACTCGTATGCCG14450.12134414037963501No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA551.8189894E-1245.041
TCGGTAA207.03339E-445.020
TAATACG5450.042.9357764
AATACGG5350.042.8971945
TTTACGG6300.042.52
TTTTACG3550.042.4647871
CGTTAGG3550.041.8309822
CGATGAA14500.041.7413819
TTACGGG11000.041.5227283
CCGATGA14450.041.26297418
GGCGACT3800.040.85526310
TTATGGG27900.040.5645143
TACGGCT22650.040.231797
CCCTCGA1400.040.17857441
TAGGGCG4150.040.1204835
TCGTTAG2300.040.1086921
TTAGGGA19450.039.9100274
TAGGGAG10950.039.8630145
TCGAATG2800.039.37500444
CCTGTCG403.4583536E-739.3759
Produced by FastQC (version 0.11.5)