SRR2934132.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934132.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793453
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3702	0.46656827814627966	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	2873	0.36208823963108083	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	2125	0.267816745289261	Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1621	0.2042969148771257	Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTATCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1310	0.16510114650773267	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCACTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	1075	0.1354837652639791	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTATCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	844	0.10637050965841706	Illumina PCR Primer Index 5 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	824	0.10384988146745933	Illumina PCR Primer Index 5 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	7.0319657E-4	45.0	45
TTTAGCG	35	1.2112832E-7	45.0	2
AAGTACG	20	7.0319657E-4	45.0	16
CGCATTA	25	3.8897095E-5	45.0	14
AACGCCC	20	7.0319657E-4	45.0	39
CGAATGC	35	1.2112832E-7	45.0	15
CGTAAGG	50	2.1827873E-11	45.0	2
GTGCGTT	20	7.0319657E-4	45.0	21
CCGTTAG	40	6.8102963E-9	45.0	1
GCGTAAG	20	7.0319657E-4	45.0	1
TCTATCG	25	3.8897095E-5	45.0	2
CCGTATG	30	2.1646483E-6	44.999996	1
TCTACGG	200	0.0	43.875	2
CGTTTAT	165	0.0	43.636364	1
TGTTACG	110	0.0	42.954544	1
CGTTAGG	235	0.0	42.12766	2
CGAGGGT	70	0.0	41.785713	4
TACGGGC	245	0.0	41.32653	4
TTCACGG	235	0.0	41.17021	2
TAGGCGT	50	1.0804797E-9	40.5	4