##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934132.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793453
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.956874572280903	33.0	31.0	34.0	30.0	34.0
2	32.184833884300645	33.0	31.0	34.0	30.0	34.0
3	32.244262735158856	34.0	31.0	34.0	30.0	34.0
4	35.67239017307894	37.0	35.0	37.0	33.0	37.0
5	29.617642128771333	37.0	35.0	37.0	0.0	37.0
6	23.726210626212264	35.0	10.0	37.0	0.0	37.0
7	30.383030878955655	35.0	19.0	37.0	17.0	37.0
8	34.257523760071486	35.0	32.0	37.0	32.0	37.0
9	36.86917183500472	38.0	37.0	39.0	34.0	39.0
10	36.969951591335594	39.0	37.0	39.0	34.0	39.0
11	37.14352204856495	39.0	37.0	39.0	34.0	39.0
12	37.17921918500529	39.0	37.0	39.0	34.0	39.0
13	37.137964063403885	39.0	37.0	39.0	33.0	39.0
14	38.28567161507991	40.0	38.0	41.0	34.0	41.0
15	38.15280048093586	40.0	37.0	41.0	33.0	41.0
16	38.3707793656335	40.0	38.0	41.0	34.0	41.0
17	38.3673500509797	40.0	38.0	41.0	34.0	41.0
18	38.43186552952727	40.0	38.0	41.0	34.0	41.0
19	38.44765096357314	40.0	38.0	41.0	34.0	41.0
20	38.42322859703095	40.0	38.0	41.0	34.0	41.0
21	38.28910219004781	40.0	37.0	41.0	34.0	41.0
22	38.34466565757518	40.0	37.0	41.0	34.0	41.0
23	38.307322550926145	40.0	37.0	41.0	34.0	41.0
24	38.30124405604365	40.0	37.0	41.0	34.0	41.0
25	38.05764550641311	40.0	37.0	41.0	34.0	41.0
26	37.92488275928127	40.0	37.0	41.0	34.0	41.0
27	37.08212836803188	40.0	36.0	41.0	33.0	41.0
28	37.47320887311536	40.0	36.0	41.0	33.0	41.0
29	37.775635103780566	40.0	36.0	41.0	33.0	41.0
30	37.77983068940441	40.0	36.0	41.0	33.0	41.0
31	37.84251745219944	40.0	37.0	41.0	33.0	41.0
32	37.8594762386682	40.0	37.0	41.0	34.0	41.0
33	36.712902969678105	40.0	36.0	41.0	32.0	41.0
34	37.204635939368806	40.0	36.0	41.0	32.0	41.0
35	37.48216718570602	40.0	36.0	41.0	32.0	41.0
36	37.57053158788233	40.0	36.0	41.0	33.0	41.0
37	37.65069890718165	40.0	36.0	41.0	33.0	41.0
38	37.62962393487705	40.0	36.0	41.0	33.0	41.0
39	37.68163961822565	40.0	36.0	41.0	33.0	41.0
40	37.62213388820762	40.0	36.0	41.0	33.0	41.0
41	37.54659948352328	40.0	36.0	41.0	33.0	41.0
42	37.583551892802724	40.0	36.0	41.0	33.0	41.0
43	37.456445435331396	40.0	36.0	41.0	33.0	41.0
44	37.44965990424134	39.0	36.0	41.0	33.0	41.0
45	37.369563162531364	39.0	35.0	41.0	33.0	41.0
46	37.30564381255096	39.0	35.0	41.0	33.0	41.0
47	37.26442145911604	39.0	35.0	41.0	33.0	41.0
48	37.21757054293071	39.0	35.0	41.0	33.0	41.0
49	37.26123916602496	39.0	35.0	41.0	33.0	41.0
50	37.17540295392418	39.0	35.0	41.0	33.0	41.0
51	36.4342550850523	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	12.0
10	14.0
11	10.0
12	4.0
13	10.0
14	23.0
15	24.0
16	22.0
17	53.0
18	96.0
19	158.0
20	282.0
21	461.0
22	754.0
23	1249.0
24	1901.0
25	2665.0
26	3669.0
27	4722.0
28	6110.0
29	8011.0
30	10967.0
31	16043.0
32	23207.0
33	37506.0
34	52506.0
35	70417.0
36	92797.0
37	141906.0
38	167708.0
39	150057.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.862290520043405	4.7091636177568175	54.984731294733265	18.443814567466504
2	20.037985866837733	4.319600530844297	53.49188924863854	22.150524353679423
3	21.078249121246	4.522133005987753	51.85650567834516	22.543112194421095
4	19.446520461829497	4.456470641613302	50.113743347116966	25.983265549440233
5	14.927160146851797	22.182788394523683	41.05271515767159	21.837336300952924
6	16.294222846217735	29.57150581067814	39.42199474953148	14.712276593572652
7	82.20272656351416	2.4455134708672097	9.623884464486238	5.7278755011323925
8	83.18400711825402	2.7173632212620027	7.840792082202727	6.25783757828126
9	77.14117912466145	5.756988756737954	10.70788061800762	6.393951500592977
10	38.687861788915036	26.49407085233782	17.573819747357437	17.24424761138971
11	25.368358302256087	24.268734253950768	26.96328579008461	23.39962165370854
12	23.236537009753572	21.190417075743618	33.555736760715504	22.017309153787306
13	22.614824066453842	23.07723330808504	33.51956574617526	20.788376879285856
14	18.72977983573066	25.146543021451805	33.60463694762009	22.519040195197448
15	16.628962269976924	23.73776392552552	37.11259520097599	22.520678603521567
16	19.279528844178547	23.09815452206999	32.958725973687166	24.6635906600643
17	19.45874550855564	23.370508398102977	30.32101460325942	26.849731490081957
18	20.465610439433714	22.368936786425913	32.83786185193074	24.327590922209634
19	22.32432166744596	23.544683806098156	30.407976275847464	23.723018250608416
20	23.726421098666208	24.586711500240092	29.86238630391466	21.82448109717904
21	23.92819738535238	24.2817154891342	30.157551865075817	21.632535260437606
22	22.47555935890343	23.626478190894733	28.59047731875738	25.30748513144446
23	21.632535260437606	24.43219699213438	28.407479712093846	25.527788035334165
24	22.23131048719962	22.89852076934614	29.229078470936525	25.64109027251772
25	21.19092120138181	24.64834085950901	28.131218862364875	26.029519076744307
26	21.70991854590001	25.590551677289014	28.204191048493104	24.495338728317872
27	20.00282310357387	26.91148688076042	30.512078220134022	22.573611795531683
28	18.959157001107815	23.22821893672341	33.37173090277559	24.440893159393184
29	20.186450867285146	21.765498397510626	32.831056155815155	25.216994579389073
30	22.34524288143091	23.307870787557675	31.021497177526587	23.32538915348483
31	22.31537343736806	24.86183806728313	28.092149125405037	24.730639369943777
32	22.307181395747445	25.016478606798387	28.751419428750037	23.924920568704135
33	21.91282911527211	25.09146729547938	28.10702083173168	24.88868275751683
34	20.746660482725503	22.37977548764703	30.582781840890387	26.290782188737076
35	21.709792514490463	22.00105110195563	30.425242578955526	25.863913804598383
36	23.575561501437388	22.40599002083299	30.005936079389706	24.012512398339915
37	19.873010751739546	23.72037159100791	32.90327215348609	23.503345503766447
38	19.444251896457633	24.222102632418054	32.13385039819624	24.199795072928076
39	22.43396899375262	22.361879027491234	30.47237832612644	24.731773652629705
40	23.726043004437567	21.69492080816381	29.465135301019718	25.113900886378904
41	20.021979877825153	22.19728200662169	29.845876189263887	27.934861926289265
42	21.013973102376575	21.229738875522557	30.930628531242554	26.82565949085831
43	21.94143824523948	20.829463118798465	31.346154088521942	25.88294454744011
44	21.360055352995072	21.511545107271633	30.96604335732551	26.16235618240778
45	22.266221187644387	20.97187861158758	29.474965750964454	27.286934449803578
46	23.311021572796374	20.789133067743144	31.734834955567624	24.165010403892857
47	19.119216891233634	21.449411622364526	34.121869852404615	25.309501633997222
48	19.51482948580445	20.649490265964083	33.8425842488465	25.99309599938497
49	21.201759902602927	19.582886446960313	33.95765092576372	25.25770272467304
50	21.363584232462415	19.415642766490265	31.825199476213463	27.39557352483386
51	19.93073313731248	19.415012609442524	29.6719528440878	30.98230140915719
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	159.0
1	439.0
2	719.0
3	1304.0
4	1889.0
5	1404.0
6	919.0
7	926.5
8	934.0
9	988.5
10	1043.0
11	1049.0
12	1055.0
13	1084.5
14	1114.0
15	1140.5
16	1167.0
17	1109.5
18	1052.0
19	1237.0
20	1422.0
21	1713.0
22	2004.0
23	2379.5
24	2755.0
25	3759.5
26	5743.5
27	6723.0
28	8153.0
29	9583.0
30	10665.0
31	11747.0
32	13779.5
33	15812.0
34	17579.5
35	19347.0
36	22034.0
37	24721.0
38	26851.5
39	28982.0
40	33459.5
41	37937.0
42	42701.5
43	47466.0
44	53649.5
45	59833.0
46	69094.5
47	78356.0
48	88579.5
49	98803.0
50	91641.5
51	84480.0
52	71443.0
53	58406.0
54	51095.5
55	43785.0
56	39417.0
57	35049.0
58	32584.0
59	30119.0
60	27284.5
61	24450.0
62	21725.0
63	19000.0
64	16566.5
65	14133.0
66	11654.5
67	9176.0
68	7591.5
69	6007.0
70	4848.5
71	3690.0
72	3047.5
73	2405.0
74	1756.0
75	839.0
76	571.0
77	452.0
78	333.0
79	318.0
80	303.0
81	199.0
82	95.0
83	61.0
84	27.0
85	17.0
86	7.0
87	4.5
88	2.0
89	1.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	793453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.23162148865105
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.37812308042261	28.002795072614873
2	11.747285584423384	9.217301231343702
3	4.483645530494554	5.277020530249332
4	2.3814427585298676	3.737114435981312
5	1.5564230445410978	3.0530499879825115
6	1.137836071470832	2.6783492443246435
7	0.9402326382120234	2.5820795681526922
8	0.7554641446570065	2.3710466697144965
9	0.6450393596963607	2.2775346004400516
>10	4.855222338806969	33.80571762014587
>50	0.0788380902828649	2.1033377323585283
>100	0.03599129354709143	2.55880716302379
>500	0.002399419569805816	0.6271955333373007
>1k	0.0020566453455478427	1.708650610330991
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3702	0.46656827814627966	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	2873	0.36208823963108083	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	2125	0.267816745289261	Illumina PCR Primer Index 5 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1621	0.2042969148771257	Illumina PCR Primer Index 5 (95% over 23bp)
CCTGTATCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	1310	0.16510114650773267	Illumina PCR Primer Index 5 (95% over 23bp)
CTGTCACTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	1075	0.1354837652639791	Illumina PCR Primer Index 5 (95% over 24bp)
TCTGTATCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	844	0.10637050965841706	Illumina PCR Primer Index 5 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	824	0.10384988146745933	Illumina PCR Primer Index 5 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2603140954788752E-4	0.0	0.0	0.3851519875783443	0.0
2	1.2603140954788752E-4	0.0	0.0	1.4169711375468994	0.0
3	1.2603140954788752E-4	0.0	0.0	1.9616788896128694	0.0
4	1.2603140954788752E-4	0.0	0.0	2.517603437128601	0.0
5	1.2603140954788752E-4	0.0	0.0	3.418728015395997	0.0
6	1.2603140954788752E-4	0.0	0.0	4.078250381560093	0.0
7	1.2603140954788752E-4	0.0	0.0	4.93450777802844	0.0
8	1.2603140954788752E-4	0.0	0.0	6.2781286352184695	0.0
9	1.2603140954788752E-4	0.0	0.0	6.881693055543303	0.0
10	1.2603140954788752E-4	0.0	0.0	8.02668841128586	0.0
11	1.2603140954788752E-4	0.0	0.0	10.36608343531375	0.0
12	1.2603140954788752E-4	0.0	0.0	12.524119261002227	0.0
13	1.2603140954788752E-4	0.0	0.0	13.1909514489201	0.0
14	1.2603140954788752E-4	0.0	0.0	13.417429891877653	0.0
15	1.2603140954788752E-4	0.0	0.0	13.774854969355463	0.0
16	1.2603140954788752E-4	0.0	0.0	14.62166001010772	0.0
17	1.2603140954788752E-4	0.0	0.0	15.874034126785077	0.0
18	1.2603140954788752E-4	0.0	0.0	17.292643672656098	0.0
19	1.2603140954788752E-4	0.0	0.0	18.18393780097876	0.0
20	1.2603140954788752E-4	0.0	0.0	19.125140367482384	0.0
21	1.2603140954788752E-4	0.0	0.0	20.40877027372762	0.0
22	1.2603140954788752E-4	0.0	0.0	21.706011572204023	0.0
23	1.2603140954788752E-4	0.0	0.0	22.888312225172758	0.0
24	1.2603140954788752E-4	0.0	0.0	23.824725598113563	0.0
25	1.2603140954788752E-4	0.0	0.0	24.634351373049192	0.0
26	1.2603140954788752E-4	0.0	0.0	25.361048480502312	0.0
27	1.2603140954788752E-4	0.0	0.0	26.05775011248303	0.0
28	1.2603140954788752E-4	0.0	0.0	26.760501252122054	0.0
29	1.2603140954788752E-4	0.0	0.0	27.546559153472227	0.0
30	2.5206281909577504E-4	0.0	0.0	28.414285408209434	0.0
31	2.5206281909577504E-4	0.0	0.0	29.199587121102322	0.0
32	3.780942286436626E-4	0.0	0.0	29.97594060391731	0.0
33	3.780942286436626E-4	0.0	0.0	30.713980538229738	0.0
34	3.780942286436626E-4	0.0	0.0	31.467018210278365	0.0
35	3.780942286436626E-4	0.0	0.0	32.28269349287229	0.0
36	3.780942286436626E-4	0.0	0.0	33.03421878800635	0.0
37	3.780942286436626E-4	0.0	0.0	33.77856029279617	0.0
38	3.780942286436626E-4	0.0	0.0	34.48937744264626	0.0
39	3.780942286436626E-4	0.0	0.0	35.24354939738082	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	7.0319657E-4	45.0	45
TTTAGCG	35	1.2112832E-7	45.0	2
AAGTACG	20	7.0319657E-4	45.0	16
CGCATTA	25	3.8897095E-5	45.0	14
AACGCCC	20	7.0319657E-4	45.0	39
CGAATGC	35	1.2112832E-7	45.0	15
CGTAAGG	50	2.1827873E-11	45.0	2
GTGCGTT	20	7.0319657E-4	45.0	21
CCGTTAG	40	6.8102963E-9	45.0	1
GCGTAAG	20	7.0319657E-4	45.0	1
TCTATCG	25	3.8897095E-5	45.0	2
CCGTATG	30	2.1646483E-6	44.999996	1
TCTACGG	200	0.0	43.875	2
CGTTTAT	165	0.0	43.636364	1
TGTTACG	110	0.0	42.954544	1
CGTTAGG	235	0.0	42.12766	2
CGAGGGT	70	0.0	41.785713	4
TACGGGC	245	0.0	41.32653	4
TTCACGG	235	0.0	41.17021	2
TAGGCGT	50	1.0804797E-9	40.5	4
>>END_MODULE