SRR2934131.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934131.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1419255
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	3659	0.2578113165005584	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	3549	0.25006077132016447	RNA PCR Primer, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2478	0.17459864506378348	RNA PCR Primer, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2374	0.16727085689322918	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	1614	0.11372163564687106	No Hit
CTGTCACTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	1461	0.1029413318959595	RNA PCR Primer, Index 16 (95% over 24bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGG	45	3.8562575E-10	45.0	3
ATCGTAC	20	7.033847E-4	45.0	11
CGTACTC	20	7.033847E-4	45.0	13
CCCGATA	30	2.1658652E-6	44.999996	25
CCGATGA	115	0.0	43.04348	18
ACGGGTA	110	0.0	40.909092	5
CGTTAGG	325	0.0	40.846153	2
CGTTATT	265	0.0	40.75472	1
GCGTTAG	105	0.0	40.714287	1
TTAGGGA	2465	0.0	40.61866	4
TATGGGA	2145	0.0	40.069927	4
TAATACG	45	1.9288564E-8	40.0	4
AATACGG	45	1.9288564E-8	40.0	5
TAGGGAT	1375	0.0	39.927273	5
TCGTTAG	175	0.0	39.857143	1
TCAAGCG	260	0.0	39.80769	17
TTATGGG	3565	0.0	39.57223	3
CCGTTAG	40	3.4589902E-7	39.375	1
AGGCACG	425	0.0	39.17647	10
TTTCGCG	305	0.0	39.098362	1