##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934131.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1419255
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98259720768995	33.0	31.0	34.0	30.0	34.0
2	32.19578229423183	33.0	31.0	34.0	30.0	34.0
3	32.26923350631141	34.0	31.0	34.0	30.0	34.0
4	35.69771816903939	37.0	35.0	37.0	35.0	37.0
5	29.54015381309208	37.0	35.0	37.0	0.0	37.0
6	23.629113866077624	35.0	10.0	37.0	0.0	37.0
7	30.34350063941998	35.0	19.0	37.0	17.0	37.0
8	34.24259417793138	35.0	32.0	37.0	32.0	37.0
9	36.89432836241549	38.0	37.0	39.0	34.0	39.0
10	37.008646790041254	39.0	37.0	39.0	34.0	39.0
11	37.16875050642767	39.0	37.0	39.0	34.0	39.0
12	37.03998788096572	39.0	37.0	39.0	34.0	39.0
13	36.92068092062385	39.0	37.0	39.0	33.0	39.0
14	37.72026732334922	40.0	37.0	41.0	33.0	41.0
15	37.68844288024351	40.0	37.0	41.0	33.0	41.0
16	38.02810488601414	40.0	37.0	41.0	33.0	41.0
17	38.088204727127966	40.0	37.0	41.0	33.0	41.0
18	38.17772352396152	40.0	37.0	41.0	34.0	41.0
19	38.19002575294785	40.0	37.0	41.0	34.0	41.0
20	38.10386505596246	40.0	37.0	41.0	33.0	41.0
21	37.98071171142607	40.0	37.0	41.0	33.0	41.0
22	38.13064882632085	40.0	37.0	41.0	34.0	41.0
23	38.110648896780354	40.0	36.0	41.0	34.0	41.0
24	38.07336666067761	40.0	36.0	41.0	34.0	41.0
25	37.84505744210871	40.0	36.0	41.0	34.0	41.0
26	37.74392480561985	40.0	36.0	41.0	33.0	41.0
27	36.913728681597036	39.0	35.0	41.0	33.0	41.0
28	37.26290765225418	39.0	35.0	41.0	33.0	41.0
29	37.53035782857908	40.0	35.0	41.0	33.0	41.0
30	37.51382521111428	39.0	35.0	41.0	33.0	41.0
31	37.483858785066815	39.0	35.0	41.0	33.0	41.0
32	37.52362049103227	39.0	35.0	41.0	33.0	41.0
33	36.35382436560026	39.0	35.0	41.0	31.0	41.0
34	36.749060598694385	39.0	35.0	41.0	31.0	41.0
35	37.06229042701981	39.0	35.0	41.0	31.0	41.0
36	37.203037509115696	39.0	35.0	41.0	32.0	41.0
37	37.27503514167644	39.0	35.0	41.0	33.0	41.0
38	37.2328806310353	39.0	35.0	41.0	33.0	41.0
39	37.2611729393238	39.0	35.0	41.0	33.0	41.0
40	37.129477789403595	39.0	35.0	41.0	33.0	41.0
41	37.0024576274172	39.0	35.0	41.0	32.0	41.0
42	37.11993686828653	39.0	35.0	41.0	33.0	41.0
43	37.074557426255325	39.0	35.0	41.0	33.0	41.0
44	37.11050163642192	39.0	35.0	41.0	33.0	41.0
45	37.03270377768618	39.0	35.0	41.0	33.0	41.0
46	36.980050801300685	39.0	35.0	40.0	33.0	41.0
47	36.91742005488795	39.0	35.0	40.0	32.0	41.0
48	36.86989793941188	39.0	35.0	40.0	32.0	41.0
49	36.90250659677084	39.0	35.0	40.0	33.0	41.0
50	36.802991710439635	39.0	35.0	40.0	32.0	41.0
51	36.04437116656274	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	7.0
10	10.0
11	25.0
12	11.0
13	23.0
14	35.0
15	52.0
16	70.0
17	123.0
18	212.0
19	381.0
20	663.0
21	1058.0
22	1764.0
23	2689.0
24	4087.0
25	5827.0
26	7819.0
27	9935.0
28	12747.0
29	16778.0
30	23235.0
31	33435.0
32	49591.0
33	82044.0
34	109343.0
35	123991.0
36	163506.0
37	245988.0
38	282394.0
39	241278.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.715322475524133	4.676890340354623	52.64656457084879	16.961222613272454
2	20.84234334210554	4.998397046337692	51.52280597919331	22.63645363236346
3	22.110896209631107	5.23056110424131	49.75187686497494	22.906665821152647
4	19.724503348587817	4.680765612944819	49.60863269814092	25.986098340326443
5	16.009948881631562	22.462700501319354	40.00620043614431	21.521150180904772
6	16.793035782857906	30.10220150712874	39.21620850375726	13.8885542062561
7	82.6000260700156	2.3765989903153413	9.894486896294183	5.128888043374869
8	83.78311156205193	3.054137558085052	7.824386738112602	5.338364141750426
9	78.31890675037255	4.799137575699927	10.365649583760494	6.516306090167025
10	41.79093961268412	25.168133985788323	17.663915223127628	15.377011178399936
11	32.70194573913779	22.46396877234887	24.958235130402922	19.875850358110416
12	28.59457955053884	20.230332110860978	29.903505712504096	21.271582626096087
13	27.823188926584724	20.274157920881024	31.685074211470106	20.217578941064147
14	21.565610126439573	22.602562612074646	34.42362366170984	21.408203599775938
15	19.20951485110146	22.22363141225502	36.556996452364096	22.009857284279427
16	25.22485388460847	22.808445275866564	29.33257237071562	22.63412846880934
17	25.223162856569115	22.698810291314807	28.424278935075094	23.65374791704098
18	26.094535513350316	21.438360266477837	30.014690806091927	22.45241341407992
19	25.676992506632	23.14531215320714	28.294281154549395	22.883414185611464
20	24.314728501925305	27.97967947972704	28.06556961222613	19.64002240612152
21	23.8091815776587	25.21428495936248	31.47369570655027	19.502837756428548
22	22.816618578056797	21.238396200823672	32.641139189222514	23.303846031897017
23	21.682713818165166	23.56968973158453	31.243786352699125	23.50381009755118
24	22.083346544489892	25.52409538807332	28.732327876244927	23.660230191191857
25	24.299156952062877	24.727409803030465	25.9839140957756	24.98951914913106
26	21.153210663340978	24.28908124332837	27.596661628812296	26.961046464518358
27	20.644774899507134	25.119657848660037	30.033151195521597	24.202416056311236
28	18.47899073809851	24.7043695459942	29.19116015092425	27.625479564983035
29	20.888071558669864	24.82309380625751	28.49734543827572	25.791489196796913
30	23.498102877918345	23.29968892130026	28.7984188887832	24.403789311998196
31	22.85191878837841	25.67600607360904	27.994475975071424	23.47759916294112
32	24.10377275401531	25.430313791390553	25.810724640744613	24.65518881384952
33	23.00784566550761	25.749002117308024	24.522302193756584	26.720850023427783
34	19.658341876547908	24.920257459018995	29.634279956737863	25.787120707695234
35	19.42378219558853	26.667441721184705	27.36252470486276	26.546251378364
36	22.577197191484263	26.13885453988184	25.642960567339905	25.640987701293987
37	20.048123839620082	28.7447992080352	25.56312995198185	25.643947000362864
38	21.195979580836426	26.96985390222335	25.320044671324037	26.514121845616184
39	20.51167690090928	23.232752394742313	25.96214210976886	30.293428594579552
40	22.488206840913012	24.0141482679293	27.792750421876267	25.70489446928142
41	19.560050871760186	25.248175979651293	26.502707406350517	28.689065742238007
42	19.53954715678296	23.997167528034076	29.10653828945468	27.35674702572829
43	20.52295042117167	22.2745736319407	29.20708399829488	27.995391948592747
44	21.429059612261362	22.059883530443788	28.34825313280559	28.162803724489255
45	20.646536387048133	21.62331645828269	26.876354143547143	30.853793011122033
46	20.633994595756224	22.47038058699811	28.908617549348076	27.987007267897596
47	17.116515354886893	25.30426174295669	31.386819141028216	26.192403761128197
48	18.14571729534157	26.48347196240281	28.47641896628865	26.89439177596697
49	21.644172470768115	23.067524863396642	28.981754512050333	26.30654815378491
50	19.51946619881558	23.522411405984126	27.325603926003435	29.632518469196867
51	18.507738214767606	24.085664662093844	25.649090543982584	31.757506579155965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	850.5
2	1452.0
3	2726.5
4	4001.0
5	2889.0
6	1777.0
7	1804.5
8	1832.0
9	1833.5
10	1835.0
11	1893.0
12	1951.0
13	2089.5
14	2228.0
15	2161.0
16	2094.0
17	2097.5
18	2101.0
19	2153.5
20	2206.0
21	2669.5
22	3133.0
23	3810.0
24	4487.0
25	5579.0
26	8466.0
27	10261.0
28	12454.0
29	14647.0
30	17294.0
31	19941.0
32	22773.5
33	25606.0
34	28590.0
35	31574.0
36	33879.5
37	36185.0
38	40306.5
39	44428.0
40	49679.0
41	54930.0
42	63049.5
43	71169.0
44	82171.5
45	93174.0
46	111775.0
47	130376.0
48	145677.5
49	160979.0
50	156779.5
51	152580.0
52	136032.0
53	119484.0
54	107873.5
55	96263.0
56	88754.0
57	81245.0
58	72624.0
59	64003.0
60	56959.0
61	49915.0
62	44463.5
63	39012.0
64	34203.0
65	29394.0
66	24876.5
67	20359.0
68	17478.0
69	14597.0
70	12025.5
71	9454.0
72	7847.0
73	6240.0
74	5150.5
75	2937.0
76	1813.0
77	1396.5
78	980.0
79	657.0
80	334.0
81	239.0
82	144.0
83	114.5
84	85.0
85	45.0
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1419255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.60083418761355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.86769586820317	25.938167857303302
2	11.091835964457427	8.119408979426298
3	4.23197012563717	4.646809105661403
4	2.3312876921231696	3.4130829705209753
5	1.5951952916031495	2.9192739182414362
6	1.2628621130519075	2.773308408097929
7	1.0555628445194998	2.704413645280474
8	0.9033286200951359	2.645006483282226
9	0.773795366798262	2.548940032379411
>10	5.7429527201486685	36.3701054445755
>50	0.08976380152946888	2.2746853847330137
>100	0.04818190651032865	3.121234292210201
>500	0.0029979844045312874	0.7860495957468153
>1k	0.0025697009181696747	1.739513882541113
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	3659	0.2578113165005584	RNA PCR Primer, Index 16 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	3549	0.25006077132016447	RNA PCR Primer, Index 16 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2478	0.17459864506378348	RNA PCR Primer, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	2374	0.16727085689322918	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	1614	0.11372163564687106	No Hit
CTGTCACTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	1461	0.1029413318959595	RNA PCR Primer, Index 16 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.409190032798898E-4	0.0	0.0	0.2729601093531465	0.0
2	1.409190032798898E-4	0.0	0.0	0.939859292375225	0.0
3	1.409190032798898E-4	0.0	0.0	1.30427583485702	0.0
4	1.409190032798898E-4	0.0	0.0	1.7292875487491677	0.0
5	1.409190032798898E-4	0.0	0.0	2.525620836283825	0.0
6	1.409190032798898E-4	0.0	0.0	3.1715230878172	0.0
7	1.409190032798898E-4	0.0	0.0	4.008441048296465	0.0
8	2.113785049198347E-4	0.0	0.0	5.241130029487302	0.0
9	2.113785049198347E-4	0.0	0.0	5.826014352600484	0.0
10	2.113785049198347E-4	0.0	0.0	6.96985390222335	0.0
11	2.818380065597796E-4	0.0	0.0	8.94004248707949	0.0
12	2.818380065597796E-4	0.0	0.0	10.75367005929167	0.0
13	2.818380065597796E-4	0.0	0.0	11.322982832542426	0.0
14	2.818380065597796E-4	0.0	0.0	11.563813409147757	0.0
15	2.818380065597796E-4	0.0	0.0	11.920690784954077	0.0
16	2.818380065597796E-4	0.0	0.0	12.621410528763331	0.0
17	2.818380065597796E-4	0.0	0.0	13.535622562541615	0.0
18	2.818380065597796E-4	0.0	0.0	14.469281418772525	0.0
19	2.818380065597796E-4	0.0	0.0	15.183529386896646	0.0
20	2.818380065597796E-4	0.0	0.0	15.930047806771862	0.0
21	2.818380065597796E-4	0.0	0.0	16.97010051047909	0.0
22	2.818380065597796E-4	0.0	0.0	18.07032562858683	0.0
23	2.818380065597796E-4	0.0	0.0	19.02765887736876	0.0
24	2.818380065597796E-4	0.0	0.0	19.79566744524416	0.0
25	2.818380065597796E-4	0.0	0.0	20.47376968902699	0.0
26	2.818380065597796E-4	0.0	0.0	21.083032999707594	0.0
27	2.818380065597796E-4	0.0	0.0	21.79093961268412	0.0
28	2.818380065597796E-4	0.0	0.0	22.44367643587657	0.0
29	2.818380065597796E-4	0.0	0.0	23.219365089430724	0.0
30	2.818380065597796E-4	0.0	0.0	24.10370229451367	0.0
31	2.818380065597796E-4	0.0	0.0	24.75841198375204	0.0
32	2.818380065597796E-4	0.0	0.0	25.386628900373786	0.0
33	3.522975081997245E-4	0.0	0.0	26.032601611408804	0.0
34	3.522975081997245E-4	0.0	0.0	26.807726588949837	0.0
35	3.522975081997245E-4	0.0	0.0	27.638162275278226	0.0
36	3.522975081997245E-4	0.0	0.0	28.304427322785546	0.0
37	3.522975081997245E-4	0.0	0.0	29.022761942004784	0.0
38	3.522975081997245E-4	0.0	0.0	29.90829695861561	0.0
39	3.522975081997245E-4	0.0	0.0	31.56099502908216	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGGG	45	3.8562575E-10	45.0	3
ATCGTAC	20	7.033847E-4	45.0	11
CGTACTC	20	7.033847E-4	45.0	13
CCCGATA	30	2.1658652E-6	44.999996	25
CCGATGA	115	0.0	43.04348	18
ACGGGTA	110	0.0	40.909092	5
CGTTAGG	325	0.0	40.846153	2
CGTTATT	265	0.0	40.75472	1
GCGTTAG	105	0.0	40.714287	1
TTAGGGA	2465	0.0	40.61866	4
TATGGGA	2145	0.0	40.069927	4
TAATACG	45	1.9288564E-8	40.0	4
AATACGG	45	1.9288564E-8	40.0	5
TAGGGAT	1375	0.0	39.927273	5
TCGTTAG	175	0.0	39.857143	1
TCAAGCG	260	0.0	39.80769	17
TTATGGG	3565	0.0	39.57223	3
CCGTTAG	40	3.4589902E-7	39.375	1
AGGCACG	425	0.0	39.17647	10
TTTCGCG	305	0.0	39.098362	1
>>END_MODULE