FastQCFastQC Report
Sat 14 Jan 2017
SRR2934130.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934130.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162199
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC113690.5258072915582701No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC107600.4976415214325786No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG95510.44172622408945705No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82070.37956728312241383No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAGAAGCT33910.15683107799050874No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT31820.14716499267643726Illumina PCR Primer Index 8 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC30780.14235507462541608RNA PCR Primer, Index 40 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC30210.1397188695397602Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTC27840.12875780628887537No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCT27300.12626034883930665No Hit
CGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTG26680.12339289769350555Illumina PCR Primer Index 8 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC26250.12140418157625639RNA PCR Primer, Index 40 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT25660.11867547806654244No Hit
GAATATGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC25620.11849048121842624No Hit
GAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT25250.11677926037335139RNA PCR Primer, Index 40 (95% over 22bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA24550.11354181553131788No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAT25250.043.752481
CGTTATT20600.043.361651
TAATACG4800.042.6562464
CGTTTTT50350.042.3634531
AATACGG4900.041.7857135
TACGAAT2450.041.3265312
CTACGGG8650.040.5780373
TTAGGGA40550.040.28364
TACGGGA11850.040.0632934
CGTTAGG4400.039.8863642
CTTGACC12400.039.7379047
TTATGGG56150.039.7105943
TCGTTAG2800.039.3751
CCGATGA12950.038.9189218
TTACGGG21050.038.9073643
TTTACGG15050.038.870432
TACGGCT18300.038.7295077
CGCTAAT356.2508443E-638.57142638
TAGGGCG9750.038.538465
TAGGGAT23150.038.488125
Produced by FastQC (version 0.11.5)