##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934130.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2162199
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93973727672615	33.0	31.0	34.0	30.0	34.0
2	32.15101385210149	33.0	31.0	34.0	30.0	34.0
3	32.21457645665362	34.0	31.0	34.0	30.0	34.0
4	35.7142899427851	37.0	35.0	37.0	35.0	37.0
5	29.243830933230473	37.0	35.0	37.0	0.0	37.0
6	23.474647338195975	35.0	10.0	37.0	0.0	37.0
7	30.291894039355306	35.0	19.0	37.0	17.0	37.0
8	34.136206704378274	35.0	32.0	37.0	32.0	37.0
9	36.87570663014829	38.0	37.0	39.0	34.0	39.0
10	37.02555315213817	39.0	37.0	39.0	34.0	39.0
11	37.18593339465979	39.0	37.0	39.0	34.0	39.0
12	37.18158828118966	39.0	37.0	39.0	34.0	39.0
13	37.135177196918505	39.0	37.0	39.0	34.0	39.0
14	38.222826853587485	40.0	38.0	41.0	33.0	41.0
15	38.0955823215162	40.0	38.0	41.0	33.0	41.0
16	38.36568743210037	40.0	38.0	41.0	34.0	41.0
17	38.36071009190181	40.0	38.0	41.0	34.0	41.0
18	38.39476199924244	40.0	38.0	41.0	34.0	41.0
19	38.363855038319784	40.0	37.0	41.0	34.0	41.0
20	38.217832863672584	40.0	37.0	41.0	34.0	41.0
21	38.125563835706146	40.0	37.0	41.0	34.0	41.0
22	38.20887716625528	40.0	37.0	41.0	34.0	41.0
23	38.11002733790923	40.0	37.0	41.0	34.0	41.0
24	38.09776713429245	40.0	37.0	41.0	34.0	41.0
25	37.868030648427826	40.0	36.0	41.0	33.0	41.0
26	37.758203569606685	40.0	36.0	41.0	33.0	41.0
27	36.82357405585702	40.0	35.0	41.0	32.0	41.0
28	37.17039920932347	40.0	35.0	41.0	32.0	41.0
29	37.460130635524294	40.0	35.0	41.0	32.0	41.0
30	37.40837591729531	39.0	35.0	41.0	33.0	41.0
31	37.36546543588263	39.0	35.0	41.0	33.0	41.0
32	37.279395189804454	39.0	35.0	41.0	32.0	41.0
33	35.880827805396265	39.0	35.0	41.0	30.0	41.0
34	36.24670393428172	39.0	35.0	41.0	29.0	41.0
35	36.50657594421235	39.0	35.0	41.0	30.0	41.0
36	36.58518711737449	39.0	35.0	41.0	31.0	41.0
37	36.6485374380434	39.0	35.0	41.0	31.0	41.0
38	36.60717908018642	39.0	35.0	41.0	31.0	41.0
39	36.60047895683977	39.0	35.0	41.0	31.0	41.0
40	36.48642793748402	39.0	35.0	41.0	31.0	41.0
41	36.372943008483496	39.0	35.0	41.0	30.0	41.0
42	36.45672391856624	39.0	35.0	41.0	31.0	41.0
43	36.348565048822984	39.0	35.0	41.0	31.0	41.0
44	36.386914432945346	39.0	35.0	41.0	31.0	41.0
45	36.292490191698356	39.0	35.0	41.0	31.0	41.0
46	36.201580428073456	39.0	35.0	40.0	30.0	41.0
47	36.09147122905893	39.0	35.0	40.0	30.0	41.0
48	36.04541718870465	39.0	35.0	40.0	30.0	41.0
49	36.05735041039238	39.0	35.0	40.0	30.0	41.0
50	35.93122187180736	38.0	35.0	40.0	30.0	41.0
51	35.18836009081495	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	10.0
10	14.0
11	24.0
12	24.0
13	27.0
14	42.0
15	80.0
16	113.0
17	223.0
18	416.0
19	981.0
20	2044.0
21	3517.0
22	5580.0
23	8396.0
24	12814.0
25	17893.0
26	22463.0
27	24142.0
28	25204.0
29	29105.0
30	36815.0
31	49780.0
32	70072.0
33	108082.0
34	148341.0
35	186949.0
36	251945.0
37	370080.0
38	423545.0
39	363198.0
40	276.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.25715764367665	4.297014289619041	50.89730408718162	18.5485239795227
2	22.345399290259593	7.878645767572736	49.16684356990268	20.609111372264994
3	19.146156297362083	7.85889735403633	52.09155124019574	20.90339510840584
4	17.27680939635991	4.357277012892893	54.03596061232106	24.32995297842613
5	16.63302036491553	23.274268464651033	40.22927584371281	19.863435326720623
6	15.58450447900494	31.722935770481808	39.39734501773426	13.29521473277899
7	78.27364641274924	2.0220617991220973	14.54449844810769	5.15979334002097
8	79.71592808987516	4.519288002630655	11.21233521983869	4.552448687655484
9	74.73562794173894	4.544216327914313	12.495334610736569	8.224821119610175
10	39.7158170917663	24.90871561775766	20.165488930482347	15.209978359993691
11	29.735237135897297	23.05375222169652	27.441738711376708	19.769271931029476
12	24.241940727934846	20.651521899695634	32.353081284377616	22.7534560879919
13	24.188985380161583	20.325649951738946	35.64815264459932	19.83721202350015
14	20.770243626974207	23.22131311687777	34.56078742058432	21.4476558355637
15	18.63135631826673	22.398215890396767	37.36159345185157	21.608834339484943
16	22.04713812188425	23.09727273021586	31.887259220821022	22.96832992707887
17	22.280419147358778	23.891094205482474	31.047789773281735	22.780696873877012
18	22.619009628623452	22.84900696004392	31.85456102791649	22.677422383416143
19	22.81482879235445	24.757526943634698	30.082568718235464	22.34507554577539
20	23.540987670422563	26.29272328772699	31.1615628348732	19.00472620697725
21	23.582288216764507	26.341377458781544	31.117487335809518	18.95884698864443
22	23.035067540036785	21.745963253151075	32.4184776701867	22.800491536625444
23	21.749431944053253	24.764556823863114	31.417043482121677	22.06896774996196
24	21.764092944266462	24.6733071285298	30.306276156819976	23.256323770383762
25	22.113043249025644	27.184176849586926	27.573780211719644	23.128999689667786
26	21.497928729039277	24.889938437673866	29.46421675340706	24.14791607987979
27	21.730377268697286	26.31843784961514	29.332730243608474	22.618454638079104
28	20.297530430825283	24.78120654019357	30.62604320878883	24.29521982019231
29	23.04366989347419	24.215439929442205	29.262061447628085	23.478828729455522
30	22.92823186024968	26.866306015311263	28.974021355111162	21.231440769327893
31	23.216179454342548	27.103240728536086	27.53312715434611	22.147452662775258
32	25.590752747550066	27.395304502499542	26.012730558103115	21.001212191847284
33	23.081363001277865	29.31631177333816	24.99224169468213	22.610083530701846
34	23.039692461239692	27.372041148848925	27.25151570230122	22.33675068761016
35	24.6916218164933	27.52475604696885	25.56411320142133	22.21950893511652
36	24.005422257618285	28.929807108411392	24.78527647085213	22.279494163118198
37	22.145510195870038	29.418245036650188	26.588995739985076	21.847249027494694
38	23.11618865793574	27.76210700310193	25.939471806249102	23.182232532713222
39	23.778616121827824	26.550655143213	26.19999361760874	23.47073511735044
40	23.772233730567816	25.802574138643113	28.169377564229748	22.255814566559323
41	21.825743143901185	26.646992251869513	26.633487481957026	24.89377712227228
42	22.906725976656173	23.87046705691752	29.075492126302898	24.147314840123414
43	22.244437260400176	24.14884106412037	28.52040908353024	25.086312591949216
44	22.667571301253954	24.131173865125273	28.125163317529978	25.07609151609079
45	22.606152347679377	23.550607506524607	27.061708936134	26.781531209662013
46	24.05264270309995	24.7326911167751	27.33342305680467	23.881243123320285
47	20.487614692264682	25.63751070091143	31.303131672894125	22.571742933929766
48	20.98816991405509	26.330693890802838	28.415377123012263	24.265759072129807
49	22.838600887337382	23.813950519818018	29.968518161371826	23.378930431472774
50	21.806827216181304	24.24906310658732	28.838141170169813	25.10596850706156
51	21.02072935932354	23.706744846334683	27.418244111665945	27.85428168267583
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	454.0
1	1209.5
2	1965.0
3	13292.0
4	24619.0
5	16922.0
6	9225.0
7	8892.5
8	8560.0
9	8722.5
10	8885.0
11	8842.0
12	8799.0
13	8650.0
14	8501.0
15	8273.5
16	8046.0
17	7578.5
18	7111.0
19	7042.5
20	6974.0
21	7251.0
22	7528.0
23	8711.0
24	9894.0
25	10963.5
26	14916.5
27	17800.0
28	20829.5
29	23859.0
30	29744.0
31	35629.0
32	38875.0
33	42121.0
34	47704.5
35	53288.0
36	58777.5
37	64267.0
38	71103.0
39	77939.0
40	84737.5
41	91536.0
42	100610.5
43	109685.0
44	128067.0
45	146449.0
46	190902.0
47	235355.0
48	227392.0
49	219429.0
50	210514.5
51	201600.0
52	176849.0
53	152098.0
54	135650.5
55	119203.0
56	111596.0
57	103989.0
58	95811.0
59	87633.0
60	80102.0
61	72571.0
62	65918.5
63	59266.0
64	52168.0
65	45070.0
66	38349.5
67	31629.0
68	25882.0
69	20135.0
70	16386.0
71	12637.0
72	10185.0
73	7733.0
74	6092.5
75	3347.0
76	2242.0
77	1750.5
78	1259.0
79	860.0
80	461.0
81	324.5
82	188.0
83	130.0
84	72.0
85	39.0
86	6.0
87	3.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2162199.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.49407680837823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.21285181860078	29.704621467009893
2	9.896890846645956	7.817371345231418
3	3.5214323811084616	4.172271628050235
4	1.9059682058439442	3.010978188637103
5	1.2894562768333402	2.546294261915068
6	0.9494558495134217	2.2498729348108246
7	0.7625567180682331	2.1081531515892133
8	0.6891437072416104	2.177367560464855
9	0.6008258231209723	2.135615508611708
>10	5.069802213119081	34.76901466477084
>50	0.06802380532698658	1.8270149270910092
>100	0.027419599949966362	1.8849763235673853
>500	0.0025839437974237074	0.6968346587865404
>1k	0.0030146010969943253	2.638231552515895
>5k	2.8710486638041196E-4	1.0067846286495308
>10k+	2.8710486638041196E-4	1.2545971982985396
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	11369	0.5258072915582701	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC	10760	0.4976415214325786	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCG	9551	0.44172622408945705	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8207	0.37956728312241383	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAAGAAGCT	3391	0.15683107799050874	No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	3182	0.14716499267643726	Illumina PCR Primer Index 8 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	3078	0.14235507462541608	RNA PCR Primer, Index 40 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	3021	0.1397188695397602	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTC	2784	0.12875780628887537	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCT	2730	0.12626034883930665	No Hit
CGCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTG	2668	0.12339289769350555	Illumina PCR Primer Index 8 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC	2625	0.12140418157625639	RNA PCR Primer, Index 40 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTT	2566	0.11867547806654244	No Hit
GAATATGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTC	2562	0.11849048121842624	No Hit
GAACTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCT	2525	0.11677926037335139	RNA PCR Primer, Index 40 (95% over 22bp)
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	2455	0.11354181553131788	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.249842405810011E-5	0.0	0.0	0.16080851022500703	0.0
2	9.249842405810011E-5	0.0	0.0	0.5601704560958543	0.0
3	9.249842405810011E-5	0.0	0.0	0.8554254256893098	0.0
4	9.249842405810011E-5	0.0	0.0	1.276894494910043	0.0
5	9.249842405810011E-5	0.0	0.0	2.4186487922712017	0.0
6	9.249842405810011E-5	0.0	0.0	3.2943776220412646	0.0
7	9.249842405810011E-5	0.0	0.0	4.302009204518178	0.0
8	9.249842405810011E-5	0.0	0.0	5.603462031015646	0.0
9	9.249842405810011E-5	0.0	0.0	6.23818621690233	0.0
10	0.002636205085655853	0.0	0.0	7.718438497104105	0.0
11	0.002636205085655853	0.0	0.0	9.241055055524491	0.0
12	0.002636205085655853	0.0	0.0	11.190459342548952	0.0
13	0.002636205085655853	0.0	0.0	11.766585776794829	0.0
14	0.002636205085655853	0.0	0.0	12.013556569029955	0.0
15	0.002728703509713953	0.0	0.0	12.395436312753821	0.0
16	0.002728703509713953	0.0	0.0	12.906675102522941	0.0
17	0.002728703509713953	0.0	0.0	13.561702692490377	0.0
18	0.002728703509713953	0.0	0.0	14.256227109530622	0.0
19	0.0027749527217430033	0.0	0.0	14.983865962383666	0.0
20	0.0028212019337720533	0.0	0.0	15.573497166542024	0.0
21	0.0028212019337720533	0.0	0.0	16.380407168812862	0.0
22	0.0028212019337720533	0.0	0.0	17.250632342351466	0.0
23	0.0028212019337720533	0.0	0.0	18.11128392900006	0.0
24	0.0028212019337720533	0.0	0.0	18.766727761875757	0.0
25	0.0028212019337720533	0.0	0.0	19.397705761588085	0.0
26	0.0028212019337720533	0.0	0.0	19.90635459548358	0.0
27	0.0028212019337720533	0.0	0.0	20.462963862253197	0.0
28	0.0028212019337720533	0.0	0.0	21.01134076928164	0.0
29	0.0028674511458011034	0.0	0.0	21.596717045933328	0.0
30	0.0028674511458011034	0.0	0.0	22.266405636113976	0.0
31	0.0029137003578301535	0.0	0.0	22.84220832587565	0.0
32	0.0029137003578301535	0.0	0.0	23.41912099672602	0.0
33	0.0029137003578301535	0.0	0.0	23.977348985916652	0.0
34	0.0029137003578301535	0.0	0.0	24.6074020013884	0.0
35	0.0029137003578301535	0.0	0.0	25.256694689064236	0.0
36	0.0029137003578301535	0.0	0.0	25.844383426317375	0.0
37	0.0029137003578301535	0.0	0.0	26.451404334198656	0.0
38	0.0029137003578301535	0.0	0.0	27.130296517573083	0.0
39	0.0029137003578301535	0.0	0.0	28.13769685398985	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTAT	2525	0.0	43.75248	1
CGTTATT	2060	0.0	43.36165	1
TAATACG	480	0.0	42.656246	4
CGTTTTT	5035	0.0	42.363453	1
AATACGG	490	0.0	41.785713	5
TACGAAT	245	0.0	41.32653	12
CTACGGG	865	0.0	40.578037	3
TTAGGGA	4055	0.0	40.2836	4
TACGGGA	1185	0.0	40.063293	4
CGTTAGG	440	0.0	39.886364	2
CTTGACC	1240	0.0	39.737904	7
TTATGGG	5615	0.0	39.710594	3
TCGTTAG	280	0.0	39.375	1
CCGATGA	1295	0.0	38.91892	18
TTACGGG	2105	0.0	38.907364	3
TTTACGG	1505	0.0	38.87043	2
TACGGCT	1830	0.0	38.729507	7
CGCTAAT	35	6.2508443E-6	38.571426	38
TAGGGCG	975	0.0	38.53846	5
TAGGGAT	2315	0.0	38.48812	5
>>END_MODULE