FastQCFastQC Report
Sat 14 Jan 2017
SRR2934129.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934129.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2677381
Sequences flagged as poor quality0
Sequence length51
%GC48

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48930.182753220404567No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT48230.18013872511980925No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC45630.17042774263356616No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC30450.1137305448869623No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC29220.10913650317231653No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAG3850.044.4155851
CGTTTAT12450.042.2891581
CGTTAGG8600.042.1220972
ACATACG2900.041.1206917
ACGGGAG18800.041.0505335
CCGTTAG1050.040.7142831
TTATGGG68300.040.4868243
TAGGGCG9950.040.251265
TATGGGA45600.039.7203944
TTAGGGA59100.039.5558364
TTACGGG28150.039.48493
CGTTATT10400.039.3750041
TAGGGAT28000.039.3755
ACGTCAC403.4605182E-739.37516
AGGGCGA15850.039.3217666
CTAGGGA32250.039.20934
ACGGGTA2300.039.1304365
TAGGGAG38900.039.1002585
TTTATGG40000.038.9812472
TTTACGG18050.038.8919642
Produced by FastQC (version 0.11.5)