##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934129.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2677381
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.994961867586273	33.0	31.0	34.0	30.0	34.0
2	32.22152319748292	33.0	31.0	34.0	30.0	34.0
3	32.290511137563165	34.0	31.0	34.0	30.0	34.0
4	35.71671308640795	37.0	35.0	37.0	35.0	37.0
5	29.22629838637086	37.0	35.0	37.0	0.0	37.0
6	23.394398854701667	35.0	8.0	37.0	0.0	37.0
7	30.29322087517615	35.0	19.0	37.0	17.0	37.0
8	34.29910311606753	35.0	32.0	37.0	32.0	37.0
9	36.94840106805867	38.0	37.0	39.0	34.0	39.0
10	37.029504205789166	39.0	37.0	39.0	34.0	39.0
11	37.199765741222485	39.0	37.0	39.0	34.0	39.0
12	37.116639731140246	39.0	37.0	39.0	34.0	39.0
13	37.03188526399493	39.0	37.0	39.0	33.0	39.0
14	37.95794808434063	40.0	37.0	41.0	33.0	41.0
15	37.875341611821405	40.0	37.0	41.0	33.0	41.0
16	38.14581114902959	40.0	37.0	41.0	33.0	41.0
17	38.1590494591543	40.0	37.0	41.0	33.0	41.0
18	38.23463115634271	40.0	37.0	41.0	34.0	41.0
19	38.222539115650704	40.0	37.0	41.0	34.0	41.0
20	38.16836341185659	40.0	37.0	41.0	34.0	41.0
21	38.050729799008806	40.0	37.0	41.0	33.0	41.0
22	38.17170100183724	40.0	37.0	41.0	34.0	41.0
23	38.12470470209507	40.0	37.0	41.0	34.0	41.0
24	38.10440239921027	40.0	36.0	41.0	34.0	41.0
25	37.873049446455326	40.0	36.0	41.0	34.0	41.0
26	37.767496669319755	40.0	36.0	41.0	33.0	41.0
27	36.89147566222364	40.0	35.0	41.0	33.0	41.0
28	37.25362172959321	40.0	35.0	41.0	33.0	41.0
29	37.5425256995549	40.0	35.0	41.0	33.0	41.0
30	37.562794387500325	39.0	35.0	41.0	33.0	41.0
31	37.553433747382236	39.0	36.0	41.0	33.0	41.0
32	37.56394700642157	39.0	36.0	41.0	33.0	41.0
33	36.3043526491	39.0	35.0	41.0	31.0	41.0
34	36.7528114228046	39.0	35.0	41.0	31.0	41.0
35	37.04118838521675	39.0	35.0	41.0	31.0	41.0
36	37.20301107686952	39.0	35.0	41.0	32.0	41.0
37	37.32291369812515	39.0	35.0	41.0	33.0	41.0
38	37.25867965747124	39.0	35.0	41.0	33.0	41.0
39	37.29702645981278	39.0	35.0	41.0	33.0	41.0
40	37.22140928018836	39.0	35.0	41.0	33.0	41.0
41	37.09831062519679	39.0	35.0	41.0	32.0	41.0
42	37.17435284705464	39.0	35.0	41.0	33.0	41.0
43	37.08824183035586	39.0	35.0	41.0	33.0	41.0
44	37.112046062924925	39.0	35.0	41.0	33.0	41.0
45	37.00820204520761	39.0	35.0	41.0	33.0	41.0
46	36.96626852883471	39.0	35.0	41.0	33.0	41.0
47	36.90913172238094	39.0	35.0	40.0	32.0	41.0
48	36.858680927368944	39.0	35.0	40.0	32.0	41.0
49	36.89395308325562	39.0	35.0	40.0	33.0	41.0
50	36.81767891831607	39.0	35.0	40.0	32.0	41.0
51	36.133481562766	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	15.0
10	20.0
11	26.0
12	29.0
13	31.0
14	47.0
15	64.0
16	108.0
17	198.0
18	353.0
19	687.0
20	1188.0
21	2015.0
22	3356.0
23	5302.0
24	8320.0
25	12486.0
26	16635.0
27	19956.0
28	24659.0
29	31488.0
30	42962.0
31	60711.0
32	89255.0
33	148255.0
34	197148.0
35	231827.0
36	306340.0
37	467255.0
38	540008.0
39	466347.0
40	286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.059638504941955	4.3937340259006845	53.836827855280966	16.7097996138764
2	21.018637242887735	3.965890547516398	51.812722955754154	23.20274925384172
3	21.924298409527818	4.429739360965063	51.17217161098851	22.47379061851862
4	19.137657285235086	4.680693558369168	49.919492220195785	26.262156936199965
5	15.316983275820661	23.00976215189396	40.804017059955235	20.869237512330148
6	16.62094412412727	29.72442099200674	39.06384634835311	14.590788535512878
7	83.57278997647329	2.147247627438904	9.434331535183077	4.845630860904742
8	85.06245468986296	2.3445673215728355	7.808862466716541	4.7841155218476565
9	79.55912886511109	4.836666877071287	10.102372430371322	5.501831827446299
10	40.28066980381201	28.045840319326988	16.68727760449484	14.986212272366167
11	31.85486114975792	22.933904438703344	25.004248554837734	20.206985856701007
12	27.889157351904714	20.920220170382922	29.91277670230722	21.277845775405144
13	26.69874029882187	20.720547430492708	32.287448069587406	20.29326420109801
14	21.2669022451418	22.82013654388374	34.33863167027778	21.574329540696674
15	19.527964081316778	22.78185286292836	35.214935789863304	22.475247265891557
16	25.16264961916141	23.113707014429398	30.309358287072328	21.41428507933686
17	24.957262339577373	23.493443779574143	29.179746924326423	22.36954695652206
18	25.87218628951203	21.162770632943165	29.97451614096014	22.990526936584672
19	24.880769677531887	24.115133408356897	28.25529874156872	22.748798172542493
20	25.66291461693349	27.59737967812575	27.371337885792123	19.368367819148638
21	24.688044025112603	26.13322496872877	29.163723803224123	20.01500720293451
22	23.20457940054105	21.92239356296321	30.88839429278089	23.984632743714844
23	22.655722140405118	24.121445546972957	30.291729118866535	22.93110319375539
24	22.82144379152612	24.365938206030446	29.440673553745246	23.371944448698187
25	24.03251535735855	24.796881728823802	25.53312360101159	25.63747931280606
26	21.768623890286815	23.958786590328383	27.895432140588134	26.377157378796667
27	20.715243740057915	24.72154691469014	29.94355304680208	24.619656298449865
28	18.308339380909928	25.24392307258474	28.665625101545128	27.782112444960205
29	20.73649585172973	25.303346815414017	28.60108441794425	25.359072914911994
30	23.758964450707612	22.413358427508076	30.248926096061783	23.578751025722525
31	22.932783940724164	23.966144527058344	28.168758947643234	24.932312584574255
32	23.15266299417229	23.550701226310338	27.994521511880453	25.302114267636917
33	22.297648336191227	25.161305021586394	27.20748373130309	25.333562910919287
34	19.90848519504695	22.4465625176245	31.000294690968527	26.644657596360027
35	18.807857380029215	25.13549621813257	29.820746468283744	26.235899933554467
36	20.185397595635436	25.333077361794977	28.592942132628863	25.888582909940723
37	19.966452290503295	25.63206357257335	27.123857232123484	27.277626904799877
38	21.27997472156559	25.673746097398915	27.13300796562013	25.913271215415364
39	21.378092994609286	23.274386424644085	28.495047959181008	26.852472621565624
40	23.058317064325173	21.17711300707669	30.6009492111881	25.163620717410033
41	18.82817574338505	22.498292174330064	28.83272870017379	29.840803382111105
42	19.58925532077803	22.40140644906347	31.13975933944403	26.869578890714468
43	20.4512917660953	21.28849050620737	31.755435629071844	26.504782098625483
44	21.376038748314116	21.938715483526625	29.47891988476799	27.20632588339127
45	21.30522327602982	20.473141476689346	28.326002164055097	29.895633083225736
46	21.154404248031938	21.02237223615167	29.283766486727142	28.53945702908925
47	17.926623069335296	24.134443323531464	31.329646396982724	26.609287210150516
48	17.787980119377856	24.868369499895607	30.248851396196507	27.094798984530033
49	21.033465166145575	21.546167691486566	30.8535094556957	26.56685768667216
50	18.964502997518846	22.569891995199786	29.460618417774683	29.004986589506686
51	18.04192231139311	21.274148132073844	28.064477935714045	32.619451620819
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	540.0
1	1459.0
2	2378.0
3	8696.0
4	15014.0
5	10132.5
6	5251.0
7	4878.5
8	4506.0
9	4564.5
10	4623.0
11	4628.0
12	4633.0
13	4797.5
14	4962.0
15	4940.0
16	4918.0
17	4834.0
18	4750.0
19	5137.0
20	5524.0
21	6264.5
22	7005.0
23	8004.0
24	9003.0
25	11390.5
26	16410.0
27	19042.0
28	21850.0
29	24658.0
30	29823.0
31	34988.0
32	40284.0
33	45580.0
34	52201.5
35	58823.0
36	63713.5
37	68604.0
38	76472.5
39	84341.0
40	92467.5
41	100594.0
42	111699.0
43	122804.0
44	144671.0
45	166538.0
46	200346.0
47	234154.0
48	265870.0
49	297586.0
50	297383.0
51	297180.0
52	270163.0
53	243146.0
54	218486.5
55	193827.0
56	172812.0
57	151797.0
58	138007.5
59	124218.0
60	111292.0
61	98366.0
62	86901.0
63	75436.0
64	64688.0
65	53940.0
66	44793.0
67	35646.0
68	29034.0
69	22422.0
70	18282.5
71	14143.0
72	12017.5
73	9892.0
74	8203.0
75	4946.0
76	3378.0
77	2568.0
78	1758.0
79	1187.5
80	617.0
81	435.0
82	253.0
83	200.5
84	148.0
85	112.5
86	77.0
87	46.0
88	15.0
89	11.5
90	8.0
91	4.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2677381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.14526042783286
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.0971437488846	25.69824776478503
2	11.94339559418072	8.633942882885874
3	4.452774760400374	4.8284011002345775
4	2.3672069048009217	3.4225324024237382
5	1.4159650020736447	2.5590211878324394
6	0.9838056866877755	2.1335947653427554
7	0.7654132043938068	1.9366241725401345
8	0.6379369426626085	1.844671754326037
9	0.521429196181788	1.696247468159965
>10	5.634869387206748	38.30177951212772
>50	0.11907025455983901	2.953058428868073
>100	0.056299208448965674	3.674514484354776
>500	0.002535194334220482	0.6687083058040154
>1k	0.0021549151840874097	1.6486557703148945
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4893	0.182753220404567	No Hit
CTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGCT	4823	0.18013872511980925	No Hit
CCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	4563	0.17042774263356616	No Hit
GCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	3045	0.1137305448869623	No Hit
TCTGTCTCTTATACACATCTGACGCTCCATCCTTCGTATGCCGTCTTCTGC	2922	0.10913650317231653	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.734993263939648E-5	0.0	0.0	0.1935473509373526	0.0
2	3.734993263939648E-5	0.0	0.0	0.6249764228550214	0.0
3	3.734993263939648E-5	0.0	0.0	0.9211613886854355	0.0
4	3.734993263939648E-5	0.0	0.0	1.2824099371736783	0.0
5	3.734993263939648E-5	0.0	0.0	1.9431675954972416	0.0
6	3.734993263939648E-5	0.0	0.0	2.730690925198916	0.0
7	3.734993263939648E-5	0.0	0.0	3.62906885497432	0.0
8	3.734993263939648E-5	0.0	0.0	5.034845619655925	0.0
9	3.734993263939648E-5	0.0	0.0	5.78464551739181	0.0
10	3.734993263939648E-5	0.0	0.0	6.962811792568932	0.0
11	3.734993263939648E-5	0.0	0.0	9.04917902980562	0.0
12	3.734993263939648E-5	0.0	0.0	10.875105186747795	0.0
13	3.734993263939648E-5	0.0	0.0	11.550952217857676	0.0
14	3.734993263939648E-5	0.0	0.0	11.814642742291815	0.0
15	3.734993263939648E-5	0.0	0.0	12.1299508736336	0.0
16	3.734993263939648E-5	0.0	0.0	12.799224316598945	0.0
17	3.734993263939648E-5	0.0	0.0	13.646843687917409	0.0
18	3.734993263939648E-5	0.0	0.0	14.541150475035119	0.0
19	3.734993263939648E-5	0.0	0.0	15.234664024283433	0.0
20	3.734993263939648E-5	0.0	0.0	16.01143057338496	0.0
21	3.734993263939648E-5	0.0	0.0	17.080684444985604	0.0
22	3.734993263939648E-5	0.0	0.0	18.21974534068928	0.0
23	3.734993263939648E-5	0.0	0.0	19.21661504283477	0.0
24	3.734993263939648E-5	0.0	0.0	19.99763948425719	0.0
25	3.734993263939648E-5	0.0	0.0	20.690630134448554	0.0
26	3.734993263939648E-5	0.0	0.0	21.29540024374566	0.0
27	3.734993263939648E-5	0.0	0.0	21.941180579080825	0.0
28	3.734993263939648E-5	0.0	0.0	22.5864006654264	0.0
29	3.734993263939648E-5	0.0	0.0	23.33164387138028	0.0
30	3.734993263939648E-5	0.0	0.0	24.292433538596114	0.0
31	3.734993263939648E-5	0.0	0.0	25.00794619816903	0.0
32	3.734993263939648E-5	0.0	0.0	25.7192383153537	0.0
33	3.734993263939648E-5	0.0	0.0	26.417532655979855	0.0
34	3.734993263939648E-5	0.0	0.0	27.150898583354405	0.0
35	3.734993263939648E-5	0.0	0.0	28.001132449957627	0.0
36	3.734993263939648E-5	0.0	0.0	28.713395665390916	0.0
37	7.469986527879296E-5	0.0	0.0	29.471599297970666	0.0
38	7.469986527879296E-5	0.0	0.0	30.38674734750116	0.0
39	7.469986527879296E-5	0.0	0.0	31.962316905961462	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAG	385	0.0	44.415585	1
CGTTTAT	1245	0.0	42.289158	1
CGTTAGG	860	0.0	42.122097	2
ACATACG	290	0.0	41.12069	17
ACGGGAG	1880	0.0	41.050533	5
CCGTTAG	105	0.0	40.714283	1
TTATGGG	6830	0.0	40.486824	3
TAGGGCG	995	0.0	40.25126	5
TATGGGA	4560	0.0	39.720394	4
TTAGGGA	5910	0.0	39.555836	4
TTACGGG	2815	0.0	39.4849	3
CGTTATT	1040	0.0	39.375004	1
TAGGGAT	2800	0.0	39.375	5
ACGTCAC	40	3.4605182E-7	39.375	16
AGGGCGA	1585	0.0	39.321766	6
CTAGGGA	3225	0.0	39.2093	4
ACGGGTA	230	0.0	39.130436	5
TAGGGAG	3890	0.0	39.100258	5
TTTATGG	4000	0.0	38.981247	2
TTTACGG	1805	0.0	38.891964	2
>>END_MODULE